Strain identifier

BacDive ID: 6584

Type strain: Yes

Species: Limosilactobacillus mucosae

Strain Designation: S32

Strain history: CIP <- 2000, CCUG <- S. Roos, SLU, Swedish Univ. Agr. Sci., Uppsala, Sweden: strain S32

NCBI tax ID(s): 1423771 (strain), 97478 (species)

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General

@ref: 5007

BacDive-ID: 6584

DSM-Number: 13345

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, Gram-positive, rod-shaped

description: Limosilactobacillus mucosae S32 is an anaerobe, mesophilic, Gram-positive bacterium that was isolated from pig small intestine.

NCBI tax id

NCBI tax idMatching level
1423771strain
97478species

strain history

@refhistory
5007<- CCUG <- S. Roos; S32
67770CIP 106485 <-- S. Roos S32.
121164CIP <- 2000, CCUG <- S. Roos, SLU, Swedish Univ. Agr. Sci., Uppsala, Sweden: strain S32

doi: 10.13145/bacdive6584.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Limosilactobacillus
  • species: Limosilactobacillus mucosae
  • full scientific name: Limosilactobacillus mucosae (Roos et al. 2000) Zheng et al. 2020

  • synonyms

    • @ref: 20215
    • synonym: Lactobacillus mucosae

@ref: 5007

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Limosilactobacillus

species: Limosilactobacillus mucosae

full scientific name: Limosilactobacillus mucosae (Roos et al. 2000) Zheng et al. 2020

strain designation: S32

type strain: yes

Morphology

cell morphology

  • @ref: 121164
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5007MRS MEDIUM (DSMZ Medium 11)yeshttps://mediadive.dsmz.de/medium/11Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
39538MEDIUM 40- for Lactobacillus and LeuconostocyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
121164CIP Medium 40yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40

culture temp

@refgrowthtypetemperaturerange
5007positivegrowth37mesophilic
39538positivegrowth37mesophilic
55710positivegrowth37mesophilic
67770positivegrowth37mesophilic
121164positivegrowth30-45
121164nogrowth10psychrophilic
121164nogrowth15psychrophilic
121164nogrowth22psychrophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 55710
  • oxygen tolerance: anaerobe

murein

  • @ref: 5007
  • murein short key: A21.04
  • type: A4ß L-Orn-D-Asp

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
6837116634raffinose+builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose-builds acid from
6837117992sucrose+builds acid from
6837128053melibiose+builds acid from
6837117716lactose+builds acid from
6837117306maltose+builds acid from
6837117057cellobiose-builds acid from
6837117814salicin-builds acid from
683714853esculin+builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
6837159640N-acetylglucosamine-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose-builds acid from
6837115824D-fructose-builds acid from
6837117634D-glucose+builds acid from
6837112936D-galactose+builds acid from
6837174863methyl beta-D-xylopyranoside+builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose+builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from
12116417632nitrate-reduction
12116416301nitrite-reduction
12116417632nitrate+respiration

antibiotic resistance

  • @ref: 121164
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite tests

  • @ref: 121164
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase+3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
121164oxidase-
121164alcohol dehydrogenase+1.1.1.1
121164catalase-1.11.1.6
121164lysine decarboxylase-4.1.1.18
121164ornithine decarboxylase-4.1.1.17

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121164--++-+----++++-++---

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
5007------+--+++-------------+--++++---+--------------
5007-----++--+++-------------+--++++---+-----------+++
5007-----++--+++-------------+--++++---+-----------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentsampling dategeographic locationisolation date
5007pig small intestineSwedenSWEEurope
55710Pig small intestine,newly slaughteredSwedenSWEEurope1997-09-01Uppsala
67770Small intestine of pig
121164Animal, Pig, small intestineSwedenSWEEuropeUppsala1997

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Suidae (Pig,Swine)
#Host Body-Site#Gastrointestinal tract#Small intestine

taxonmaps

  • @ref: 69479
  • File name: preview.99_2427.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_45;96_1311;97_1558;98_1895;99_2427&stattab=map
  • Last taxonomy: Limosilactobacillus mucosae subclade
  • 16S sequence: LC383823
  • Sequence Identity:
  • Total samples: 105505
  • soil counts: 2576
  • aquatic counts: 7206
  • animal counts: 94221
  • plant counts: 1502

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
50071Risk group (German classification)
1211641Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
5007Lactobacillus mucosae 16S ribosomal RNA gene, complete sequenceAF1267381568ena97478
67770Lactobacillus mucosae JCM 12515 gene for 16S ribosomal RNA, partial sequenceLC3838231515ena97478

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Lactobacillus mucosae DSM 133451423771.3wgspatric1423771
66792Limosilactobacillus mucosae DSM 133452657245411draftimg1423771
67770Limosilactobacillus mucosae DSM 13345GCA_001436025contigncbi1423771

GC content

@refGC-contentmethod
500746high performance liquid chromatography (HPLC)
6777046.3-46.7high performance liquid chromatography (HPLC)
6777046.4genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno97.029no
gram-positiveyes96.744no
anaerobicno51.204yes
halophileyes88.702no
spore-formingno96.802no
thermophileno99.314yes
glucose-utilyes89.856no
aerobicno97.571yes
flagellatedno98.397no
glucose-fermentyes87.724no

External links

@ref: 5007

culture collection no.: DSM 13345, CCUG 43179, CIP 106485, JCM 12515, KCTC 21011, LMG 19534, NCIMB 13705

straininfo link

  • @ref: 76058
  • straininfo: 13438

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny16085805Development and assessment of a real-time pcr assay for rapid and sensitive detection of a novel thermotolerant bacterium, Lactobacillus thermotolerans, in chicken feces.Selim AS, Boonkumklao P, Sone T, Assavanig A, Wada M, Yokota AAppl Environ Microbiol10.1128/AEM.71.8.4214-4219.20052005Animals, Chickens/*microbiology, Colony Count, Microbial, DNA Primers, DNA, Bacterial/analysis, Feces/*microbiology, *Hot Temperature, Lactobacillus/classification/genetics/*growth & development/*isolation & purification, Polymerase Chain Reaction/*methods, Species Specificity, Time FactorsStress
Enzymology25519527Effects of the probiotic Enterococcus faecium NCIMB 10415 on selected lactic acid bacteria and enterobacteria in co-culture.Starke IC, Zentek J, Vahjen WBenef Microbes10.3920/BM2014.00522015Animals, Coculture Techniques, Enterococcus/chemistry/genetics/*growth & development, Enterococcus faecium/chemistry/genetics/*growth & development, Gastrointestinal Tract/microbiology, Kinetics, Lactobacillus/chemistry/genetics/*growth & development, Probiotics/*chemistry, Swine

Reference

@idauthorscataloguedoi/urltitle
5007Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 13345)https://www.dsmz.de/collection/catalogue/details/culture/DSM-13345
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
39538Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/18620
55710Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 43179)https://www.ccug.se/strain?id=43179
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
76058Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID13438.1StrainInfo: A central database for resolving microbial strain identifiers
121164Curators of the CIPCollection of Institut Pasteur (CIP 106485)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106485