Lactobacillus sp. (DSM 20354, ATCC 27744, IFO 13120)

Name: Lactobacillus sp. (DSM 20354, ATCC 27744, IFO 13120)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 6400

Type strain

Strain Designation S-24

Culture col. no. DSM 20354 ATCC 27744 IFO 13120 NBRC 13120 S-24

NCBI tax ID(s) 1591

Special Collections DSMZ

Links

S-24 is a mesophilic prokaryote that was isolated from spoilt sake.

mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 39152

ATCC 27744,IFO 13120,DSM 20354,LMG 22711,NBRC 13120

@ref 20215

Last LPSN update 2025-12-16 09:48:19

Domain Bacillati

Phylum Bacillota

Class Bacilli

Order Lactobacillales

Family Lactobacillaceae

Genus Lactobacillus

Full scientific name Lactobacillus Beijerinck 1901 (Approved Lists 1980)

Morphology

Cell morphology

@ref	Motility	Confidence	Gram stain
125438		93
125438		94.552	positive

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
8616	LACTOBACILLUS MEDIUM II (DSMZ Medium 93)		Medium recipe at MediaDive	Name: LACTOBACILLUS MEDIUM II (DSMZ Medium 93) Composition: Na-acetate 20.0 g/l Glucose 20.0 g/l Agar 15.0 g/l Casein peptone 10.0 g/l Yeast extract 5.0 g/l Meat extract 2.0 g/l K2HPO4 0.5 g/l KH2PO4 0.5 g/l MgSO4 x 7 H2O 0.2 g/l DL-mevalonic acid 0.03 g/l FeSO4 x 7 H2O 0.01 g/l MnSO4 x H2O 0.0075 g/l Tween 80 Ethanol Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
8616	positive	growth	26	mesophilic

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
125439	obligate aerobe	93.8

Spore formation

@ref	Spore formation	Confidence
125438		91.568

Murein

@ref	Murein short key	Type
8616	A11.31	A4alpha L-Lys-D-Asp

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Food production	#Fermented
#Environmental	#Microbial community	-
#Engineered	#Food production	#Beverage

Isolation

8616

Sample typespoilt sake

Taxonmaps

69479

Global distribution of 16S sequence AB680370 (>99% sequence identity) for Lactobacillus acetotolerans subclade from Microbeatlas

Aquatic Samples	1136
Soil Samples	1475
Animal Samples	6619
Plant Samples	398
Total Samples	9628

Interaction and safety

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM104240v1 assembly for Lactobacillus acetotolerans NBRC 13120	complete	GCA_001042405	1600.4	2651869842	1600	98.59

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	Fructilactobacillus fructivorans NBRC 13120 gene for 16S rRNA, partial sequence	AB680370	1495		33961

GC content

8616

GC-content (mol%)34.6

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Lactobacillus acetotolerans NBRC 13120
NCBI: GCA_001042405

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	53.30	no
125439	motility	BacteriaNetⓘ	yes	53.50	no
125439	gram_stain	BacteriaNetⓘ	positive	77.20	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	93.80	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Lactobacillus acetotolerans NBRC 13120
NCBI: GCA_001042405.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	94.55	no
125438	anaerobic	anaerobicⓘ	no	65.63	no
125438	aerobic	aerobicⓘ	no	96.02	no
125438	spore-forming	spore-formingⓘ	no	91.57	no
125438	thermophilic	thermophileⓘ	no	97.00	yes
125438	flagellated	motile2+ⓘ	no	93.00	no

Literature

References

#8616	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 20354
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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