Halorubrum ezzemoulense 5'1, 5.1 | Type strain | DSM 17463, CECT 7099 | BacDiveID:5947

Strain identifier

BacDive ID: 5947

Type strain:

Species: Halorubrum ezzemoulense

Strain Designation: 5'1, 5.1

Culture col. no.: DSM 17463, CECT 7099

Strain history: <- M. Monteoliva-Sánchez, Depart. Microbiol., Facultad de Farmacia, Campus Univ. de Cartuja s/n, Spain; 5'1 <- K. Kharroub

NCBI tax ID(s): 1121945 (strain), 337243 (species)

SI-ID 297206

CECT 7099, DSM 17463

Other strains from Halorubrum ezzemoulense

H. ezzemoulense Halo-G*, HaloG, DSM 19316, ATCC ...
H. ezzemoulense aus-1, DSM 11365, JCM 9573

Section

Halorubrum ezzemoulense 5'1 is an aerobe, mesophilic, Gram-negative archaeon that was isolated from water samples from Ezzemoul sabkha.

Gram-negative
motile
ovoid-shaped
aerobe
mesophilic
16S sequence
Archaea
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Archaea"
	Phylum	*Methanobacteriota*
	Class	*Halobacteria*
	Order	*Haloferacales*
	Family	*Halorubraceae*
	Genus	*Halorubrum*
	Species	**Halorubrum ezzemoulense**
	Full Scientific Name (LPSN)	Halorubrum ezzemoulense Kharroub et al. 2006

Synonym

Halorubrum chaoviator

Information on morphological and physiological properties Morphology

[Ref.: #31786]	Gram stain	negative

[Ref.: #31786]	Cell length	1.5-3 µm

[Ref.: #31786]	Cell width	0.6 µm

[Ref.: #31786]	Cell shape	ovoid-shaped

[Ref.: #31786]	Motility	yes

Pigment production

yes

Multimedia content

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Information on culture and growth conditions Culture and growth conditions

Culture medium

HALOBACTERIA MEDIUM (DSMZ Medium 372)

Culture medium growth

Culture medium link

Medium recipe at MediaDive

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #7002]			growth	37 °C
[Ref.: #31786]			growth	22-50 °C
[Ref.: #31786]			optimum	37-40 °C

Temperature range

mesophilic

	pH	Kind of pH		pH
[Ref.: #31786]			optimum	7-7.5
[Ref.: #31786]			growth	6.5-9

Information on physiology and metabolism Physiology and metabolism

Oxygen tolerance

aerobe

Ability of spore formation

no

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #31786]		NaCl		growth	15-25	%
[Ref.: #31786]		NaCl		optimum	20	%

Observation

aggregates in chains

	Metabolite utilization	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #31786]		acetate ChEBI	+	carbon source
[Ref.: #31786]		arabinose ChEBI	+	carbon source
[Ref.: #31786]		citrate ChEBI	+	carbon source
[Ref.: #31786]		glucose ChEBI	+	carbon source
[Ref.: #31786]		glycerol ChEBI	+	carbon source
[Ref.: #31786]		malate ChEBI	+	carbon source
[Ref.: #31786]		maltose ChEBI	+	carbon source
[Ref.: #31786]		mannitol ChEBI	+	carbon source
[Ref.: #31786]		nitrate ChEBI	+	reduction
[Ref.: #31786]		sucrose ChEBI	+	carbon source

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #31786]		catalase	+	1.11.1.6
[Ref.: #31786]		cytochrome oxidase	+	1.9.3.1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #7002]	Sample type/isolated from	water samples from Ezzemoul sabkha
[Ref.: #7002]	Geographic location (country and/or sea, region)	Ezzemoul sabkha, in north-east Algeria
[Ref.: #7002]	Country	Algeria
[Ref.: #7002]	Country ISO 3 Code	DZA
[Ref.: #7002]	Continent	Africa
* marker position based on {}

Isolation sources categories

#Environmental

#Aquatic

-

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

Biosafety level

1

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #7002]	Halorubrum ezzemoulense strain 5.1 16S ribosomal RNA gene, partial sequence	DQ118426	1405	ENA	337243 tax ID
[Ref.: #20218]	Halorubrum ezzemoulense gene for 16S ribosomal RNA, partial sequence	AB576126	1470	ENA	337243 tax ID	*
[Ref.: #20218]	Halorubrum ezzemoulense gene for 16S rRNA, complete sequence, strain: CECT 7099	AB663412	1470	ENA	337243 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Halorubrum ezzemoulense DSM 17463	GCA_000421805	contig	GenBank	1121945 tax ID	*
[Ref.: #66792]	Halorubrum ezzemoulense DSM 17463	GCA_002114285	contig	GenBank	1121945 tax ID	*
[Ref.: #66792]	Halorubrum ezzemoulense DSM 17463	2524614679	draft	JGI IMG/M	1121945 tax ID	*
[Ref.: #66792]	Halorubrum ezzemoulense DSM 17463 strain DSM 17463	1121945.5	wgs	PATRIC	1121945 tax ID	*
[Ref.: #66792]	Halorubrum ezzemoulense DSM 17463	1121945.3	wgs	PATRIC	1121945 tax ID	*

GC-content

61.9 mol%

Availability in culture collections External links

Culture collection no.

DSM 17463, CECT 7099

SI-ID 297206

*

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Halorubrum chaoviator Mancinelli et al. 2009 is a later, heterotypic synonym of Halorubrum ezzemoulense Kharroub et al. 2006. Emended description of Halorubrum ezzemoulense Kharroub et al. 2006.	Corral P, de la Haba RR, Infante-Dominguez C, Sanchez-Porro C, Amoozegar MA, Papke RT, Ventosa A	Int J Syst Evol Microbiol	10.1099/ijsem.0.003005	2018	*
Phylogeny	Halorubrum ezzemoulense sp. nov., a halophilic archaeon isolated from Ezzemoul sabkha, Algeria.	Kharroub K, Quesada T, Ferrer R, Fuentes S, Aguilera M, Boulahrouf A, Ramos-Cormenzana A, Monteoliva-Sanchez M	Int J Syst Evol Microbiol	10.1099/ijs.0.64272-0	2006	*

References

#7002

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 17463

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#31786

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #28058 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#66793

Mukherjee et al.: GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life. 35: 676 - 683 2017 ( DOI 10.1038/nbt.3886 , PubMed 28604660 )

#75436

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID297206.1 )

#28058

IJSEM 1583 2006 ( DOI 10.1099/ijs.0.64272-0 , PubMed 16825633 )

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.