Name: Halorubrum distributum 4p
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 5946

Type strain:

Species: Halorubrum distributum

Strain Designation: 4p

Culture col. no.: DSM 23453, JCM 10247, CGMCC 1.5397, CGMCC 1.8971, CIP 108318, VKM B-1739

Strain history: CIP <- 2004, JCM <- 1998, VKM <- I. S. Zvyagintseva: strain 4p

NCBI tax ID(s): 267368 (species)

SI-ID 389474

JCM 10247, VKM B-1739, CIP 108318, DSM 23453

Other strains from Halorubrum distributum

H. distributum CIP 105238, ATCC 51197, JCM 9100, ... (type strain)
H. distributum DSM 23454, JCM 13916, CGMCC 1.5343, ...
H. distributum Fa-1, DSM 23497, CGMCC 1.5336, ...

Section

Halorubrum distributum 4p is a mesophilic, Gram-negative, ovoid-shaped archaeon of the family Halorubraceae.

Gram-negative
ovoid-shaped
mesophilic
16S sequence
Archaea
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Archaea"
	Phylum	*Methanobacteriota*
	Class	*Halobacteria*
	Order	*Haloferacales*
	Family	*Halorubraceae*
	Genus	*Halorubrum*
	Species	**Halorubrum distributum**
	Full Scientific Name (LPSN)	Halorubrum distributum (Zvyagintseva and Tarasov 1989) Oren and Ventosa 1996

	Synonym	Halobacterium distributus
	Synonym	Halorubrum terrestre
	Synonym	Halorubrum arcis
	Synonym	Halorubrum litoreum
	Synonym	Halorubrobacterium distributum
	Synonym	Halobacterium distributum

Information on morphological and physiological properties Morphology

[Ref.: #123471]	Gram stain	negative

[Ref.: #123471]	Cell shape	ovoid-shaped

[Ref.: #123471]	Motility	no

Information on culture and growth conditions Culture and growth conditions

[Ref.: #17218]

Culture medium

HALOBACTERIA MEDIUM (DSMZ Medium 372)

[Ref.: #17218]

Culture medium growth

[Ref.: #17218]

Culture medium link

Medium recipe at MediaDive

[Ref.: #17218]

Culture medium composition

expand / minimize

[Ref.: #39994]

Culture medium

MEDIUM 223 - for Halorubrum saccharovorum and H. distributum

[Ref.: #39994]

Culture medium growth

[Ref.: #39994]

Culture medium composition

expand / minimize

[Ref.: #123471]

Culture medium

CIP Medium 223

[Ref.: #123471]

Culture medium growth

[Ref.: #123471]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #123471]

Culture medium

CIP Medium 330

[Ref.: #123471]

Culture medium growth

[Ref.: #123471]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #17218]		growth	37 °C
[Ref.: #39994]		growth	37 °C
[Ref.: #67770]		growth	37 °C
[Ref.: #123471]		growth	25-45 °C
[Ref.: #123471]	no	growth	5 °C
[Ref.: #123471]	no	growth	15 °C

[Ref.: #17218]	Temperature range	mesophilic
[Ref.: #39994]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #123471]	Temperature range	psychrophilic

Information on physiology and metabolism Physiology and metabolism

	Salt	Tested relation		Salt conc.
[Ref.: #123471]	NaCl	no	growth	0	%
[Ref.: #123471]	NaCl	no	growth	2	%
[Ref.: #123471]	NaCl	no	growth	4	%
[Ref.: #123471]	NaCl	no	growth	6	%
[Ref.: #123471]	NaCl	no	growth	8	%
[Ref.: #123471]	NaCl	no	growth	10	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #123471]	hippurate ChEBI	+	hydrolysis
[Ref.: #123471]	nitrate ChEBI	-	reduction
[Ref.: #123471]	nitrite ChEBI	-	reduction

	Metabolite production	Metabolite	Production
[Ref.: #123471]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #123471]		acetoin ChEBI	-
[Ref.: #123471]		glucose ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #123471]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	+	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #123471]	beta-galactosidase	-	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #123471]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #123471]	gamma-glutamyltransferase	-	2.3.2.2
[Ref.: #123471]	gelatinase	-
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #123471]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #123471]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #123471]	oxidase	+
[Ref.: #123471]	phenylalanine ammonia-lyase	-	4.3.1.24
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #123471]	tryptophan deaminase	-
[Ref.: #123471]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #123471]	11083	-	+	+	+	-	+	-	-	-	+	-	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #67770]	Sample type/isolated from	Salt crust
[Ref.: #67770]	Geographic location (country and/or sea, region)	Ashgabat
[Ref.: #67770]	Country	Turkmenistan
[Ref.: #67770]	Country ISO 3 Code	TKM
[Ref.: #67770]	Continent	Asia

[Ref.: #123471]	Sample type/isolated from	Salt crust
[Ref.: #123471]	Geographic location (country and/or sea, region)	Ashkhabad
[Ref.: #123471]	Country	Turkmenistan
[Ref.: #123471]	Country ISO 3 Code	TKM
[Ref.: #123471]	Continent	Asia

[Ref.: #39994]	Geographic location (country and/or sea, region)	Ashkhabad
[Ref.: #39994]	Country	USSR
[Ref.: #39994]	Country ISO 3 Code	TKM
* marker position based on {}

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #17218]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #123471]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Halobacterium distributum 16S ribosomal RNA (16S rRNA)	L26117	182	ENA	29283 tax ID
[Ref.: #20218]	Halorubrum terrestre gene for 16S rRNA, complete sequence, strain: JCM 10247	AB663422	1470	ENA	267368 tax ID	*
[Ref.: #20218]	Halorubrum terrestre gene for 16S rRNA, partial sequence	AB090169	1467	ENA	267368 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Halorubrum terrestre JCM 10247	GCA_000337435	contig	GenBank	1227486 tax ID
[Ref.: #66792]	Halorubrum terrestre JCM 10247	2554235509	draft	JGI IMG/M	1227486 tax ID	*
[Ref.: #66792]	Halorubrum terrestre JCM 10247	1227486.3	wgs	PATRIC	1227486 tax ID	*

[Ref.: #67770]

GC-content

64.4 mol%

thermal denaturation, midpoint method (Tm)

Availability in culture collections External links

[Ref.: #17218]

Culture collection no.

DSM 23453, JCM 10247, CGMCC 1.5397, CGMCC 1.8971, CIP 108318, VKM B-1739

[Ref.: #75435]

SI-ID 389474

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Genome-based analyses reveal a synonymy among Halorubrum distributum Zvyagintseva and Tarasov 1989; Oren and Ventosa 1996, Halorubrum terrestre Ventosa et al. 2004, Halorubrum arcis Xu et al. 2007 and Halorubrum litoreum Cui et al. 2007. Emended description of Halorubrum distributum Zvyagintseva and Tarasov 1989; Oren and Ventosa 1996.	Infante-Dominguez C, de la Haba RR, Corral P, Sanchez-Porro C, Arahal DR, Ventosa A	Int J Syst Evol Microbiol	10.1099/ijsem.0.003956	2020	*
Phylogeny	Taxonomic study of Halorubrum distributum and proposal of Halorubrum terrestre sp. nov.	Ventosa A, Gutierrez MC, Kamekura M, Zvyagintseva IS, Oren A	Int J Syst Evol Microbiol	10.1099/ijs.0.02621-0	2004	*
Phylogeny	[A new description of the type strain of Halobacterium distributum].	Zviagintseva IS, Kudriashova EB, Bulygina ES	Mikrobiologiia		1996	*

References

#17218

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 23453

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#39994 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68382

Automatically annotated from API zym .

#75435

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID389474.1 )

#123471 Collection of Institut Pasteur ; Curators of the CIP; CIP 108318
* These data were automatically processed and therefore are not curated

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