Enterococcus faecalis (DSM 20371, 27738)
Enterococcus faecalis 27738 is a microaerophile bacterium that was isolated from pleural fluid.
microaerophile BacteriaName and taxonomic classification
| @ref 20215 |
Last LPSN update
2026-05-29 08:26:08 |
| Domain Bacteria |
| Phylum Bacillota |
| Class Bacilli |
| Order Lactobacillales |
| Family Enterococcaceae |
| Genus Enterococcus |
| Species Enterococcus faecalis |
| Full scientific name Enterococcus faecalis (Andrewes and Horder 1906) Schleifer and Kilpper-Bälz 1984 |
| Synonyms (1) |
Culture and growth conditions
Culture medium
| @ref | Name | Growth | Medium link | Composition | |
|---|---|---|---|---|---|
| 8625 | COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) | Medium recipe at MediaDive  | Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base | ||
| 8625 | TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) | Medium recipe at MediaDive | Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water |
Culture temp
| @ref | Growth | Type | Temperature (°C) | |
|---|---|---|---|---|
| 8625 | positive | growth | 37 |
Physiology and metabolism
Oxygen tolerance
| 8625 | Oxygen tolerancemicroaerophile |
Murein
| @ref | Murein short key | Type | |
|---|---|---|---|
| 8625 | A11.06 | A3alpha L-Lys-L-Ala3 |
Metabolite utilization
| @ref | Chebi-ID | Metabolite | Utilization activity | Kind of utilization tested | |
|---|---|---|---|---|---|
| 68381 | 40585 ChEBI | alpha-cyclodextrin | + | builds acid from | from API rID32STR |
| 68381 | 29016 ChEBI | arginine | + | hydrolysis | from API rID32STR |
| 68381 | 18333 ChEBI | D-arabitol | - | builds acid from | from API rID32STR |
| 68381 | 16899 ChEBI | D-mannitol | + | builds acid from | from API rID32STR |
| 68381 | 16988 ChEBI | D-ribose | + | builds acid from | from API rID32STR |
| 68381 | 16443 ChEBI | D-tagatose | + | builds acid from | from API rID32STR |
| 68381 | 28087 ChEBI | glycogen | - | builds acid from | from API rID32STR |
| 68381 | 606565 ChEBI | hippurate | + | hydrolysis | from API rID32STR |
| 68381 | 30849 ChEBI | L-arabinose | - | builds acid from | from API rID32STR |
| 68381 | 17716 ChEBI | lactose | + | builds acid from | from API rID32STR |
| 68381 | 17306 ChEBI | maltose | + | builds acid from | from API rID32STR |
| 68381 | 6731 ChEBI | melezitose | + | builds acid from | from API rID32STR |
| 68381 | 28053 ChEBI | melibiose | - | builds acid from | from API rID32STR |
| 68381 | 320055 ChEBI | methyl beta-D-glucopyranoside | + | builds acid from | from API rID32STR |
| 68381 | 27941 ChEBI | pullulan | - | builds acid from | from API rID32STR |
| 68381 | 16634 ChEBI | raffinose | - | builds acid from | from API rID32STR |
| 68381 | 30911 ChEBI | sorbitol | + | builds acid from | from API rID32STR |
| 68381 | 17992 ChEBI | sucrose | + | builds acid from | from API rID32STR |
| 68381 | 27082 ChEBI | trehalose | + | builds acid from | from API rID32STR |
| 68381 | 16199 ChEBI | urea | - | hydrolysis | from API rID32STR |
Metabolite production
| @ref | Chebi-ID | Metabolite | Production | |
|---|---|---|---|---|
| 68381 | 15688 ChEBI | acetoin | from API rID32STR |
Metabolite tests
| @ref | Chebi-ID | Metabolite | Voges-proskauer-test | |
|---|---|---|---|---|
| 68381 | 15688 ChEBI | acetoin | + | from API rID32STR |
Enzymes
| @ref | Value | Activity | Ec | |
|---|---|---|---|---|
| 68381 | Alanyl-Phenylalanyl-Proline arylamidase | - | from API rID32STR | |
| 68381 | alpha-galactosidase | - | 3.2.1.22 | from API rID32STR |
| 68381 | arginine dihydrolase | + | 3.5.3.6 | from API rID32STR |
| 68381 | beta-galactosidase | - | 3.2.1.23 | from API rID32STR |
| 68381 | beta-glucosidase | + | 3.2.1.21 | from API rID32STR |
| 68381 | beta-glucuronidase | - | 3.2.1.31 | from API rID32STR |
| 68381 | glycyl tryptophan arylamidase | + | from API rID32STR | |
| 68381 | N-acetyl-beta-glucosaminidase | + | 3.2.1.52 | from API rID32STR |
| 68381 | pyrrolidonyl arylamidase | + | 3.4.19.3 | from API rID32STR |
| 68381 | urease | - | 3.5.1.5 | from API rID32STR |
API rID32STR
| @ref | ADH (Arg) | beta GLU | beta GAR | beta GUR | alpha GAL | PAL | RIB | MAN | SOR | LAC | TRE | RAF | SAC | LARA | DARL | Acid from alpha-cyclodextrinCDEX | Acetoin production (Voges Proskauer test)VP | Alanyl-Phenylalanyl-Proline arylamidaseAPPA | beta GAL | Pyrrolidonyl arylamidasePyrA | N-Acetyl-glucosaminidasebeta NAG | Glycyl-tryptophan arylamidaseGTA | HIP | GLYG | PUL | MAL | MEL | MLZ | Acidification of methyl beta-D-glucopyranosideMbeta DG | TAG | beta MAN | URE | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 8625 | + | + | - | - | - | +/- | + | + | + | + | + | - | + | - | - | + | + | - | - | + | + | + | + | - | - | + | - | + | + | + | +/- | - |
Literature
Literature 
| Topic | Title | Authors | Journal | DOI | Year | |
|---|---|---|---|---|---|---|
| Measurement of Bacterial Headspaces by FT-IR Spectroscopy Reveals Distinct Volatile Organic Compound Signatures. | Zenner C, Hall LJ, Roy S, Hauer J, Sroka R, Maiti KS. | Anal Chem | 10.1021/acs.analchem.4c02899 | 2025 | | |
| Identification of Bacteria Associated with Post-Operative Wounds of Patients with the Use of Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Approach. | Szultka-Mlynska M, Janiszewska D, Pomastowski P, Zloch M, Kupczyk W, Buszewski B. | Molecules | 10.3390/molecules26165007 | 2021 | | |
| Proteome | Identification of Microorganisms by Liquid Chromatography-Mass Spectrometry (LC-MS1) and in Silico Peptide Mass Libraries. | Lasch P, Schneider A, Blumenscheit C, Doellinger J. | Mol Cell Proteomics | 10.1074/mcp.tir120.002061 | 2020 | |
| Structural diversity and biological significance of lipoteichoic acid in Gram-positive bacteria: focusing on beneficial probiotic lactic acid bacteria. | Shiraishi T, Yokota S, Fukiya S, Yokota A. | Biosci Microbiota Food Health | 10.12938/bmfh.2016-006 | 2016 | | |
| Systematic review of membrane components of gram-positive bacteria responsible as pyrogens for inducing human monocyte/macrophage cytokine release. | Rockel C, Hartung T. | Front Pharmacol | 10.3389/fphar.2012.00056 | 2012 | | |
| Enzymology | Evaluation of a novel real-time PCR test based on the ssrA gene for the identification of group B streptococci in vaginal swabs. | Wernecke M, Mullen C, Sharma V, Morrison J, Barry T, Maher M, Smith T. | BMC Infect Dis | 10.1186/1471-2334-9-148 | 2009 | |
| Enzymology | Separation of the poly(glycerophosphate) lipoteichoic acids of Enterococcus faecalis Kiel 27738, Enterococcus hirae ATCC 9790 and Leuconostoc mesenteroides DSM 20343 into molecular species by affinity chromatography on concanavalin A. | Leopold K, Fischer W | Eur J Biochem | 10.1111/j.1432-1033.1991.tb15839.x | 1991 | |
How to cite
If you want to cite this particular strain cite the following doi:
https://doi.org/10.13145/bacdive5288.20260601.11
When using BacDive for research please cite the following paper
BacDive in 2025: the core database for prokaryotic strain dataIsabel Schober, Julia Koblitz, Joaquim Sardà Carbasse, Christian Ebeling, Marvin Leon Schmidt, Adam Podstawka, Rohit Gupta, Vinodh Ilangovan, Javad Chamanara, Jörg Overmann, Lorenz Christian Reimer
Nucleic Acids Research; database issue 2025.
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