Name: Enterococcus faecalis 1528
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 5285

Type strain:

Species: Enterococcus faecalis

Strain Designation: 1528, vanA, RKI

Culture col. no.: DSM 13591, CIP 110054, WDCM 00153, CECT 8225, NCTC 13632

Strain history: CIP <- 2009, DSMZ <- G. Reuter : strain 1528 <- Witte ad I. Klare: strain RKI <- E. Halle, Univ. Klinikum Charite, Berlin, Germany : strain vanA

NCBI tax ID(s): 1351 (species)

SI-ID 143344

DSM 13591, CIP 110054, CECT 8225

Other strains from Enterococcus faecalis

Section

Enterococcus faecalis 1528 is a microaerophile, mesophilic, Gram-positive bacterium that has multiple antibiotic resistances and was isolated from human eye.

antibiotic resistance
coccus-shaped
Gram-positive
mesophilic
microaerophile
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Lactobacillales*
	Family	*Enterococcaceae*
	Genus	*Enterococcus*
	Species	**Enterococcus faecalis**
	Full Scientific Name (LPSN)	Enterococcus faecalis (Andrewes and Horder 1906) Schleifer and Kilpper-Bälz 1984

Synonym

Streptococcus faecalis

Information on morphological and physiological properties Morphology

[Ref.: #119166]	Gram stain	positive

[Ref.: #119166]	Cell shape	coccus-shaped

[Ref.: #119166]	Motility	no

[Ref.: #5099]	Type of hemolysis	gamma
[Ref.: #5099]	Incubation period	1-2 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #5099]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #5099]

Culture medium growth

[Ref.: #5099]

Culture medium link

Medium recipe at MediaDive

[Ref.: #5099]

Culture medium composition

expand / minimize

[Ref.: #5099]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

[Ref.: #5099]

Culture medium growth

[Ref.: #5099]

Culture medium link

Medium recipe at MediaDive

[Ref.: #5099]

Culture medium composition

expand / minimize

[Ref.: #41822]

Culture medium

MEDIUM 29- Brain heart agar

[Ref.: #41822]

Culture medium growth

[Ref.: #41822]

Culture medium composition

expand / minimize

[Ref.: #119166]

Culture medium

CIP Medium 29

[Ref.: #119166]

Culture medium growth

[Ref.: #119166]

Culture medium link

Medium recipe provided by DSMZ

	Temperatures	Kind of temperature		Temperature
[Ref.: #5099]			growth	37 °C
[Ref.: #41822]			growth	30 °C

[Ref.: #5099]	Temperature range	mesophilic
[Ref.: #41822]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #5099]	Oxygen tolerance	microaerophile
[Ref.: #119166]	Oxygen tolerance	facultative anaerobe

Note that the displayed test results represent raw data and therefore may deviate!

Antibiotic susceptibility testing

Test 1

Reference

[Ref.: #5099]
Mueller-Hinton Agar
37

microaerophil
yes
Inhibition zone diameter in mm
24
10-12
28-30
26-28
30
16
16
24
resistant0
30
6
24
resistant0
6
10
resistant0
resistant0
20
20
resistant0
resistant0
24
18
resistant0
resistant0
resistant0
14
16-18
resistant0
24-26
28
30
resistant0
10
10
26

Only first 15 entries are displayed. Click here to see all.

[Ref.: #5099]

Observation

Vancomycin resistant

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68381]	alpha-cyclodextrin ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #68381]	arginine ChEBI	+	hydrolysis	* from API rID32STR
[Ref.: #68381]	D-arabitol ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	D-mannitol ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #68381]	D-ribose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #68381]	D-tagatose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #68381]	glycogen ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	L-arabinose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	lactose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #68381]	maltose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #68381]	melezitose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #68381]	melibiose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	methyl beta-D-glucopyranoside ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #119166]	nitrate ChEBI	-	reduction
[Ref.: #119166]	nitrite ChEBI	-	reduction
[Ref.: #68381]	pullulan ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	sorbitol ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #68381]	sucrose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #68381]	trehalose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #68381]	urea ChEBI	-	hydrolysis	* from API rID32STR
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #119166]		indole ChEBI	no
[Ref.: #68381]		acetoin ChEBI	yes	* from API rID32STR

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #68381]		acetoin ChEBI	+	* from API rID32STR

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68381]	Alanyl-Phenylalanyl-Proline arylamidase	-		* from API rID32STR
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	+	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym, API rID32STR
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68381]	arginine dihydrolase	+	3.5.3.6	* from API rID32STR
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68381]	beta-galactosidase	-	3.2.1.23	* from API rID32STR
[Ref.: #68381]	beta-glucosidase	+	3.2.1.21	* from API rID32STR
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym, API rID32STR
[Ref.: #68381]	beta-mannosidase	+	3.2.1.25	* from API rID32STR
[Ref.: #5099]	catalase	+	1.11.1.6
[Ref.: #119166]	catalase	-	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #5099]	cytochrome-c oxidase	-	1.9.3.1
[Ref.: #68382]	esterase (C 4)	-		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68381]	glycyl tryptophan arylamidase	+		* from API rID32STR
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym, API rID32STR
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #119166]	oxidase	-
[Ref.: #68381]	pyrrolidonyl arylamidase	+	3.4.19.3	* from API rID32STR
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #119166]	urease	-	3.5.1.5
[Ref.: #68381]	urease	-	3.5.1.5	* from API rID32STR
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® rID32STR:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Arginine dihydrolaseADH (Arg)	beta-Glucosidasebeta GLU	beta-Galactosidasebeta GAR	beta-Glucuronidasebeta GUR	alpha-Galactosidasealpha GAL	Alkaline PhosphatasePAL	Acid from D-riboseRIB	Acid from D-mannitolMAN	Acid from sorbitolSOR	Acid from lactoseLAC	Acid from trehaloseTRE	Acid from raffinoseRAF	Acid from sucroseSAC	Acid from L-arabinoseLARA	Acid from D-arabitolDARL	Acid from alpha-cyclodextrinCDEX	Acetoin production (Voges Proskauer test)VP	Alanyl-Phenylalanyl-Proline arylamidaseAPPA	beta-Galactosidasebeta GAL	Pyrrolidonyl arylamidasePyrA	N-Acetyl-glucosaminidasebeta NAG	Glycyl-tryptophan arylamidaseGTA	Hydrolysis of hippuric acidHIP	Acid from glycogenGLYG	Acid from pullulanPUL	Acid from maltoseMAL	Acid from melibioseMEL	Acid from melezitoseMLZ	Acidification of methyl beta-D-glucopyranosideMbeta DG	Acid from D-tagatoseTAG	beta-Mannosidasebeta MAN	Urease/urea hydrolysisURE
[Ref.: #5099]	10284	+	+	+	-	-	+	+	+	+	+	+	-	+	-	-	+	+	-	-	+	+	+	+	-	-	+	-	+	+	+	+	-
[Ref.: #5099]	10283	+	+	-	-	-	-	+	+	+	+	+	-	+	-	-	+	+	-	-	+	+	+	-	-	-	+	-	+	+	+	+	-
[Ref.: #5099]	10282	+	+	-	-	-	+	+	+	+	+	+	-	+	-	-	+	+	-	-	+	+	+	-	-	-	+	-	+	+	+	+	-
[Ref.: #5099]	10912	+	+	-	-	-	+	+	+	+	+	+	+	+	-	-	+	+	-	-	+	+	+	+	-	-	+	-	+	+	+	+	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #119166]	12328	-	-	-	+	-	+	-	-	-	+	+	+	-	+	-	+	-	+	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #5099]	Sample type/isolated from	human eye
[Ref.: #5099]	Geographic location (country and/or sea, region)	Berlin
[Ref.: #5099]	Country	Germany
[Ref.: #5099]	Country ISO 3 Code	DEU
[Ref.: #5099]	Continent	Europe

[Ref.: #119166]	Sample type/isolated from	Human, Eye
* marker position based on {}

Isolation sources categories

#Host	#Human	-
#Host Body-Site	#Organ	#Eye

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #5099]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #119166]	Biosafety level	2	Risk group (French classification)

Availability in culture collections External links

[Ref.: #5099]

Culture collection no.

DSM 13591, CIP 110054, WDCM 00153, CECT 8225, NCTC 13632

[Ref.: #74775]

SI-ID 143344

References

#5099

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 13591

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#41822 ; Curators of the CIP;

#68381

Automatically annotated from API rID32STR .

#68382

Automatically annotated from API zym .

#74775

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID143344.1 )

#119166 Collection of Institut Pasteur ; Curators of the CIP; CIP 110054
* These data were automatically processed and therefore are not curated

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