Name: Cronobacter dublinensis subsp. dublinensis CFS237
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 5239

Type strain:

Species: Cronobacter dublinensis subsp. dublinensis

Strain Designation: CFS237

Culture col. no.: CCUG 55851, DSM 18705, LMG 23823, DES 187, JCM 16467

Strain history: LMG 23823 <-- A. Lehner DES187 <-- C. Iversen <-- N. Mullane.

NCBI tax ID(s): 1159554 (strain), 413497 (species)

Section

Cronobacter dublinensis subsp. dublinensis CFS237 is a facultative anaerobe, mesophilic, Gram-negative bacterium that was isolated from milk powder production facility.

Gram-negative
motile
rod-shaped
facultative anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Enterobacterales*
	Family	*Enterobacteriaceae*
	Genus	*Cronobacter*
	Species	**Cronobacter dublinensis subsp. dublinensis**
	Full Scientific Name (PNU)	Cronobacter dublinensis subsp. dublinensis Iversen et al. 2008

Information on morphological and physiological properties Morphology

[Ref.: #32494]	Gram stain	negative
[Ref.: #32494]	Cell length	3 µm
[Ref.: #32494]	Cell width	1 µm
[Ref.: #32494]	Cell shape	rod-shaped
[Ref.: #32494]	Motility	yes

[Ref.: #69480]	Gram stain	negative Confidence in %99.868

[Ref.: #69480]	Motility	yes Confidence in %95.929

Information on culture and growth conditions Culture and growth conditions

[Ref.: #7666]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #7666]

Culture medium growth

[Ref.: #7666]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #7666]

Culture medium composition

expand / minimize

[Ref.: #7666]

Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

[Ref.: #7666]

Culture medium growth

[Ref.: #7666]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #7666]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #7666]	growth	37 °C
[Ref.: #32494]	growth	06-45 °C
[Ref.: #60756]	growth	30 °C
[Ref.: #67770]	growth	30 °C

[Ref.: #7666]	Temperature range	mesophilic
[Ref.: #60756]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #32494]			growth	05-10

Information on physiology and metabolism Physiology and metabolism

[Ref.: #32494]	Oxygen tolerance	facultative anaerobe
[Ref.: #60756]	Oxygen tolerance	aerobe

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.596

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #32494]		NaCl		growth	<7	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68368]	amygdalin ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	arginine ChEBI	+	hydrolysis	* from API 20E
[Ref.: #68368]	citrate ChEBI	+	assimilation	* from API 20E
[Ref.: #68368]	D-glucose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	D-mannitol ChEBI	+	fermentation	* from API 20E
[Ref.: #32494]	esculin ChEBI	+	hydrolysis
[Ref.: #68368]	gelatin ChEBI	+	hydrolysis	* from API 20E
[Ref.: #68368]	L-arabinose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	L-rhamnose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	lysine ChEBI	-	degradation	* from API 20E
[Ref.: #32494]	malonate ChEBI	+	carbon source
[Ref.: #68368]	melibiose ChEBI	+	fermentation	* from API 20E
[Ref.: #32494]	myo-inositol ChEBI	+	carbon source
[Ref.: #68368]	myo-inositol ChEBI	+	fermentation	* from API 20E
[Ref.: #32494]	nitrate ChEBI	+	reduction
[Ref.: #68368]	ornithine ChEBI	+	degradation	* from API 20E
[Ref.: #32494]	putrescine ChEBI	+	carbon source
[Ref.: #68368]	sorbitol ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	sucrose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	tryptophan ChEBI	+	energy source	* from API 20E
[Ref.: #68368]	urea ChEBI	-	hydrolysis	* from API 20E
	Only first 10 entries are displayed. Click here to see all.

	Metabolite
[Ref.: #32494]	indole ChEBI
[Ref.: #68368]	acetoin ChEBI	* from API 20E
[Ref.: #68368]	hydrogen sulfide ChEBI	* from API 20E
[Ref.: #68368]	indole ChEBI	* from API 20E

	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #32494]	indole ChEBI		+
[Ref.: #68368]	acetoin ChEBI	+		* from API 20E
[Ref.: #68368]	indole ChEBI		+	* from API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #68368]	arginine dihydrolase	+	3.5.3.6	* from API 20E
[Ref.: #68368]	beta-galactosidase	+	3.2.1.23	* from API 20E
[Ref.: #32494]	catalase	+	1.11.1.6
[Ref.: #68368]	cytochrome oxidase	-	1.9.3.1	* from API 20E
[Ref.: #68368]	gelatinase	+		* from API 20E
[Ref.: #68368]	lysine decarboxylase	-	4.1.1.18	* from API 20E
[Ref.: #68368]	ornithine decarboxylase	+	4.1.1.17	* from API 20E
[Ref.: #68368]	tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #68368]	urease	-	3.5.1.5	* from API 20E

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #7666]	15345	+	+	-	+	+	-	-	-	+	+	+	+	+	+	-	+	+	+	+	+	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.
[Ref.: #7666]	14017	+	+	-	+	+	-	-	-	+	+	+	+	+	+	-	+	+	+	+	+	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #7666]	Sample type/isolated from	milk powder production facility
[Ref.: #7666]	Geographic location (country and/or sea, region)	Dublin
[Ref.: #7666]	Country	Ireland
[Ref.: #7666]	Country ISO 3 Code	IRL
[Ref.: #7666]	Continent	Europe

[Ref.: #60756]	Sample type/isolated from	Milk powder production facility
[Ref.: #60756]	Geographic location (country and/or sea, region)	Dublin
[Ref.: #60756]	Country	Ireland
[Ref.: #60756]	Country ISO 3 Code	IRL
[Ref.: #60756]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Milk powder manufacturing facility
* marker position based on {}

Isolation sources categories

#Engineered	#Food production	#Dairy product
#Engineered	#Industrial	#Plant (Factory)
#Host Body Product	#Fluids	#Milk

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence EF059892 (>99% sequence identity) for Cronobacter from Microbeatlas

Aquatic Samples	2553
Soil Samples	1166
Animal Samples	20787
Plant Samples	2029
Total Samples	26535

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #7666]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #7666]	Cronobacter dublinensis subsp. dublinensis LMG 23823 strain DES187 16S ribosomal RNA gene, partial sequence	EF059892	1361	ENA	1159554 tax ID

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Cronobacter dublinensis subsp. dublinensis LMG 23823, complete genome	CP012266		ENA	1159554 tax ID
[Ref.: #67770]	Cronobacter dublinensis subsp. dublinensis LMG 23823 LMG 23823	GCA_000409225	contig	GenBank	1159554 tax ID
[Ref.: #66792]	Cronobacter dublinensis subsp. dublinensis LMG 23823	1159554.4	wgs	PATRIC	1159554 tax ID	*
[Ref.: #66792]	Cronobacter dublinensis subsp. dublinensis LMG 23823	1159554.10	plasmid	PATRIC	1159554 tax ID	*
[Ref.: #66792]	Cronobacter dublinensis subsp. dublinensis LMG 23823	1159554.7	complete	PATRIC	1159554 tax ID	*
[Ref.: #66792]	Cronobacter dublinensis dublinensis LMG 23823	2663762850	complete	JGI IMG/M	1159554 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Cronobacter dublinensis subsp. dublinensis LMG 23823 LMG 23823 NCBI: GCA_001277235.1
[Ref.: #69480]	gram-positive	no	99.868	no
[Ref.: #69480]	spore-forming	no	99.596	yes
[Ref.: #69480]	anaerobic	no	99.169	no
[Ref.: #69480]	thermophile	no	99.487	yes
[Ref.: #69480]	motile	yes	95.929	yes

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Cronobacter dublinensis subsp. dublinensis LMG 23823 LMG 23823 NCBI: GCA_001277235.1
[Ref.: #69481]	spore-forming	no	100	no

Availability in culture collections External links

[Ref.: #7666]

Culture collection no.

CCUG 55851, DSM 18705, LMG 23823, DES 187, JCM 16467

[Ref.: #20218]

Associated Passport(s) in StrainInfo

823184, 823185

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Cronobacter condimenti sp. nov., isolated from spiced meat, and Cronobacter universalis sp. nov., a species designation for Cronobacter sp. genomospecies 1, recovered from a leg infection, water and food ingredients.	Joseph S, Cetinkaya E, Drahovska H, Levican A, Figueras MJ, Forsythe SJ	Int J Syst Evol Microbiol	10.1099/ijs.0.032292-0	2011	*
Phylogeny	Cronobacter gen. nov., a new genus to accommodate the biogroups of Enterobacter sakazakii, and proposal of Cronobacter sakazakii gen. nov., comb. nov., Cronobacter malonaticus sp. nov., Cronobacter turicensis sp. nov., Cronobacter muytjensii sp. nov., Cronobacter dublinensis sp. nov., Cronobacter genomospecies 1, and of three subspecies, Cronobacter dublinensis subsp. dublinensis subsp. nov., Cronobacter dublinensis subsp. lausannensis subsp. nov. and Cronobacter dublinensis subsp. lactaridi subsp. nov.	Iversen C, Mullane N, McCardell B, Tall BD, Lehner A, Fanning S, Stephan R, Joosten H	Int J Syst Evol Microbiol	10.1099/ijs.0.65577-0	2008	*

References

#7666

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 18705

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#32494

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #28714 (see below)

#60756 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 55851

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68368

Automatically annotated from API 20E .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#28714

IJSEM 1442 2008 ( DOI 10.1099/ijs.0.65577-0 , PubMed 18523192 )

* These data were automatically processed and therefore are not curated

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Cronobacter dublinensis subsp. dublinensis LMG 23823 LMG 23823

Cronobacter dublinensis subsp. dublinensis LMG 23823 LMG 23823

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