Strain identifier

BacDive ID: 5239

Type strain: Yes

Species: Cronobacter dublinensis

Strain Designation: CFS237

Strain history: LMG 23823 <-- A. Lehner DES187 <-- C. Iversen <-- N. Mullane.

NCBI tax ID(s): 1159554 (strain), 413497 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 7666

BacDive-ID: 5239

DSM-Number: 18705

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Cronobacter dublinensis CFS237 is a facultative anaerobe, mesophilic, Gram-negative bacterium that was isolated from milk powder production facility.

NCBI tax id

NCBI tax idMatching level
413497species
1159554strain

strain history

@refhistory
7666<- A. Lehner; CFS237 <- C. Iversen, Nestec S. A. <- N. Mullane, Dublin, Ireland
67770LMG 23823 <-- A. Lehner DES187 <-- C. Iversen <-- N. Mullane.

doi: 10.13145/bacdive5239.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Enterobacterales
  • family: Enterobacteriaceae
  • genus: Cronobacter
  • species: Cronobacter dublinensis
  • full scientific name: Cronobacter dublinensis Iversen et al. 2008

  • synonyms

    • @ref: 20215
    • synonym: Cronobacter dublinensis

@ref: 7666

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Enterobacteriales, not validated

family: Enterobacteriaceae

genus: Cronobacter

species: Cronobacter dublinensis subsp. dublinensis

full scientific name: Cronobacter dublinensis subsp. dublinensis Iversen et al. 2008

strain designation: CFS237

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
32494negative3 µm1 µmrod-shapedyes
69480yes95.929
69480negative99.868

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
7666COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
7666TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
7666positivegrowth37mesophilic
32494positivegrowth06-45
60756positivegrowth30mesophilic
67770positivegrowth30mesophilic

culture pH

  • @ref: 32494
  • ability: positive
  • type: growth
  • pH: 05-10
  • PH range: alkaliphile

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
32494facultative anaerobe
60756aerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.596

halophily

  • @ref: 32494
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: <7 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3249415792malonate+carbon source
3249417268myo-inositol+carbon source
3249417148putrescine+carbon source
324944853esculin+hydrolysis
3249417632nitrate+reduction
6836830849L-arabinose+fermentation
6836827613amygdalin+fermentation
6836828053melibiose+fermentation
6836817992sucrose+fermentation
6836862345L-rhamnose+fermentation
6836830911sorbitol-fermentation
6836817268myo-inositol+fermentation
6836816899D-mannitol+fermentation
6836817634D-glucose+fermentation
683685291gelatin+hydrolysis
6836827897tryptophan+energy source
6836816199urea-hydrolysis
6836816947citrate+assimilation
6836818257ornithine+degradation
6836825094lysine-degradation
6836829016arginine+hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
3249435581indoleyes
6836815688acetoinyes
6836835581indoleyes
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetaboliteindole testvoges-proskauer-test
3249435581indole+
6836815688acetoin+
6836835581indole+

enzymes

@refvalueactivityec
32494catalase+1.11.1.6
68368cytochrome oxidase-1.9.3.1
68368gelatinase+
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase+4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase+3.5.3.6
68368beta-galactosidase+3.2.1.23

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARAOX
7666++-++---++++++-+++++-
7666++-++---++++++-+++++-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
7666milk powder production facilityDublinIrelandIRLEurope
60756Milk powder production facilityDublinIrelandIRLEurope
67770Milk powder manufacturing facility

isolation source categories

Cat1Cat2Cat3
#Engineered#Food production#Dairy product
#Engineered#Industrial#Plant (Factory)
#Host Body Product#Fluids#Milk

taxonmaps

  • @ref: 69479
  • File name: preview.99_317.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_6;96_7;97_241;98_270;99_317&stattab=map
  • Last taxonomy: Cronobacter
  • 16S sequence: EF059892
  • Sequence Identity:
  • Total samples: 26535
  • soil counts: 1166
  • aquatic counts: 2553
  • animal counts: 20787
  • plant counts: 2029

Safety information

risk assessment

  • @ref: 7666
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 7666
  • description: Cronobacter dublinensis subsp. dublinensis LMG 23823 strain DES187 16S ribosomal RNA gene, partial sequence
  • accession: EF059892
  • length: 1361
  • database: ena
  • NCBI tax ID: 1159554

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Cronobacter dublinensis subsp. dublinensis LMG 23823GCA_001277235completencbi1159554
66792Cronobacter dublinensis subsp. dublinensis LMG 238231159554.4wgspatric1159554
66792Cronobacter dublinensis subsp. dublinensis LMG 238231159554.10plasmidpatric1159554
66792Cronobacter dublinensis subsp. dublinensis LMG 238231159554.7completepatric1159554
66792Cronobacter dublinensis dublinensis LMG 238232663762850completeimg1159554
66792Cronobacter dublinensis dublinensis LMG 238232548876988draftimg1159554
67770Cronobacter dublinensis subsp. dublinensis LMG 23823GCA_000409225contigncbi1159554

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileyes92.191yes
flagellatedno78.919no
gram-positiveno97.823yes
anaerobicno96.346no
halophileno77.575yes
spore-formingno93.701no
thermophileno99.331yes
glucose-utilyes95.608no
aerobicyes75.903no
glucose-fermentyes92.852yes

External links

@ref: 7666

culture collection no.: CCUG 55851, DSM 18705, LMG 23823, DES 187, JCM 16467

straininfo link

  • @ref: 74730
  • straininfo: 354304

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny18523192Cronobacter gen. nov., a new genus to accommodate the biogroups of Enterobacter sakazakii, and proposal of Cronobacter sakazakii gen. nov., comb. nov., Cronobacter malonaticus sp. nov., Cronobacter turicensis sp. nov., Cronobacter muytjensii sp. nov., Cronobacter dublinensis sp. nov., Cronobacter genomospecies 1, and of three subspecies, Cronobacter dublinensis subsp. dublinensis subsp. nov., Cronobacter dublinensis subsp. lausannensis subsp. nov. and Cronobacter dublinensis subsp. lactaridi subsp. nov.Iversen C, Mullane N, McCardell B, Tall BD, Lehner A, Fanning S, Stephan R, Joosten HInt J Syst Evol Microbiol10.1099/ijs.0.65577-02008Bacterial Typing Techniques, Cronobacter sakazakii/*classification/genetics/metabolism/physiology, DNA, Bacterial/analysis, Enterobacteriaceae/*classification/genetics/metabolism/physiology, Genes, rRNA, Malonates/metabolism, Microarray Analysis, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, RNA, Ribosomal, 16S/genetics, Reagent Kits, Diagnostic, Sequence Analysis, DNA, Species SpecificityMetabolism
Phylogeny22661070Cronobacter condimenti sp. nov., isolated from spiced meat, and Cronobacter universalis sp. nov., a species designation for Cronobacter sp. genomospecies 1, recovered from a leg infection, water and food ingredients.Joseph S, Cetinkaya E, Drahovska H, Levican A, Figueras MJ, Forsythe SJInt J Syst Evol Microbiol10.1099/ijs.0.032292-02011Child, Cronobacter/*classification/genetics/*isolation & purification, DNA, Bacterial/genetics, Enterobacteriaceae Infections/*microbiology, *Food Microbiology, Humans, Leg/*microbiology, Male, Meat Products/*microbiology, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Slovakia, *Water MicrobiologyBiotechnology

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
7666Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 18705)https://www.dsmz.de/collection/catalogue/details/culture/DSM-18705
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
32494Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2871428776041
60756Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 55851)https://www.ccug.se/strain?id=55851
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
74730Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID354304.1StrainInfo: A central database for resolving microbial strain identifiers