Name: Cronobacter turicensis 3032
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 5238

Type strain:

Species: Cronobacter turicensis

Strain Designation: 3032

Culture col. no.: DSM 18703, LMG 23827, CCUG 55852

Strain history: <- A. Lehner; z3032 <- S. Roger, University Zürich

NCBI tax ID(s): 693216 (strain), 413502 (species)

SI-ID 354308

DSM 18703, LMG 23827, CCUG 55852

Section

Cronobacter turicensis 3032 is a facultative anaerobe, mesophilic, Gram-negative bacterium that was isolated from neonate with meningitidis.

Gram-negative
motile
rod-shaped
facultative anaerobe
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Enterobacterales*
	Family	*Enterobacteriaceae*
	Genus	*Cronobacter*
	Species	**Cronobacter turicensis**
	Full Scientific Name (LPSN)	Cronobacter turicensis Iversen et al. 2008

Synonym

"Cronobacter turicensis"

Information on morphological and physiological properties Morphology

[Ref.: #32494]	Gram stain	negative

[Ref.: #32494]	Cell length	3 µm

[Ref.: #32494]	Cell width	1 µm

[Ref.: #32494]	Cell shape	rod-shaped

[Ref.: #32494]	Motility	yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #7664]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #7664]

Culture medium growth

[Ref.: #7664]

Culture medium link

Medium recipe at MediaDive

[Ref.: #7664]

Culture medium composition

expand / minimize

[Ref.: #7664]

Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

[Ref.: #7664]

Culture medium growth

[Ref.: #7664]

Culture medium link

Medium recipe at MediaDive

[Ref.: #7664]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #7664]			growth	37 °C
[Ref.: #32494]			growth	06-45 °C

[Ref.: #7664]

Temperature range

mesophilic

	pH	Kind of pH		pH
[Ref.: #32494]			growth	05-10

Information on physiology and metabolism Physiology and metabolism

[Ref.: #32494]

Oxygen tolerance

facultative anaerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #32494]		NaCl		growth	<7	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68368]	amygdalin ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	arginine ChEBI	+	hydrolysis	* from API 20E
[Ref.: #68368]	citrate ChEBI	+	assimilation	* from API 20E
[Ref.: #68368]	D-glucose ChEBI	+	fermentation	* from API 20E
[Ref.: #32494]	esculin ChEBI	+	hydrolysis
[Ref.: #68368]	L-arabinose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	L-rhamnose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	lysine ChEBI	-	degradation	* from API 20E
[Ref.: #32494]	malonate ChEBI	+	carbon source
[Ref.: #68368]	melibiose ChEBI	+	fermentation	* from API 20E
[Ref.: #32494]	myo-inositol ChEBI	+	carbon source
[Ref.: #68368]	myo-inositol ChEBI	+	fermentation	* from API 20E
[Ref.: #32494]	nitrate ChEBI	+	reduction
[Ref.: #68368]	ornithine ChEBI	+	degradation	* from API 20E
[Ref.: #32494]	putrescine ChEBI	+	carbon source
[Ref.: #68368]	sorbitol ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	tryptophan ChEBI	-	energy source	* from API 20E
[Ref.: #68368]	urea ChEBI	-	hydrolysis	* from API 20E
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #68368]	acetoin ChEBI	yes	* from API 20E
[Ref.: #68368]	hydrogen sulfide ChEBI	no	* from API 20E
[Ref.: #68368]	indole ChEBI	no	* from API 20E

	Physiological tests	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #68368]		acetoin ChEBI	+		* from API 20E
[Ref.: #68368]		indole ChEBI		-	* from API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #68368]	arginine dihydrolase	+	3.5.3.6	* from API 20E
[Ref.: #32494]	catalase	+	1.11.1.6
[Ref.: #68368]	cytochrome oxidase	-	1.9.3.1	* from API 20E
[Ref.: #68368]	lysine decarboxylase	-	4.1.1.18	* from API 20E
[Ref.: #68368]	ornithine decarboxylase	+	4.1.1.17	* from API 20E
[Ref.: #68368]	tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #68368]	urease	-	3.5.1.5	* from API 20E

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #7664]	17941	-	+	-	+	+	-	-	-	-	+	+	+	+/-	+	-	+	-	+	+	+	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.
[Ref.: #7664]	16610	+	+	-	+	+	-	-	-	-	+	+/-	+	+	+	-	+	+	+	+	+	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #7664]	Sample type/isolated from	neonate with meningitidis
[Ref.: #7664]	Geographic location (country and/or sea, region)	Zürich
[Ref.: #7664]	Country	Switzerland
[Ref.: #7664]	Country ISO 3 Code	CHE
[Ref.: #7664]	Continent	Europe

[Ref.: #60757]	Sample type/isolated from	Human blood,neonatal,meningitis
[Ref.: #60757]	Sampling date	2005
[Ref.: #60757]	Geographic location (country and/or sea, region)	Zürich
[Ref.: #60757]	Country	Switzerland
[Ref.: #60757]	Country ISO 3 Code	CHE
[Ref.: #60757]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Infection	#Disease	#Meningitis
#Infection	#Patient	-
#Host	#Human	#Child

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence EF059891 (>99% sequence identity) for Cronobacter from Microbeatlas

Aquatic Samples	2553
Soil Samples	1166
Animal Samples	20787
Plant Samples	2029
Total Samples	26535

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #7664]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #7664]	Cronobacter turicensis z3032 16S ribosomal RNA gene, partial sequence	EF059891	1423	ENA	693216 tax ID

Availability in culture collections External links

[Ref.: #7664]

Culture collection no.

DSM 18703, LMG 23827, CCUG 55852

[Ref.: #74729]

SI-ID 354308

Literature:

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Interaction of matrix metalloproteinase-9 and Zpx in Cronobacter turicensis LMG 23827(T) mediated infections in the zebrafish model.	Eshwar AK, Wolfrum N, Stephan R, Fanning S, Lehner A	Cell Microbiol	10.1111/cmi.12888	2018	*
Pathogenicity	Evaluation of zebrafish as a model to study the pathogenesis of the opportunistic pathogen Cronobacter turicensis.	Fehr A, Eshwar AK, Neuhauss SC, Ruetten M, Lehner A, Vaughan L	Emerg Microbes Infect	10.1038/emi.2015.29	2015	*
Phylogeny	Cronobacter condimenti sp. nov., isolated from spiced meat, and Cronobacter universalis sp. nov., a species designation for Cronobacter sp. genomospecies 1, recovered from a leg infection, water and food ingredients.	Joseph S, Cetinkaya E, Drahovska H, Levican A, Figueras MJ, Forsythe SJ	Int J Syst Evol Microbiol	10.1099/ijs.0.032292-0	2011	*
Genetics	Complete genome sequence of Cronobacter turicensis LMG 23827, a food-borne pathogen causing deaths in neonates.	Stephan R, Lehner A, Tischler P, Rattei T	J Bacteriol	10.1128/JB.01162-10	2010	*
Phylogeny	Cronobacter gen. nov., a new genus to accommodate the biogroups of Enterobacter sakazakii, and proposal of Cronobacter sakazakii gen. nov., comb. nov., Cronobacter malonaticus sp. nov., Cronobacter turicensis sp. nov., Cronobacter muytjensii sp. nov., Cronobacter dublinensis sp. nov., Cronobacter genomospecies 1, and of three subspecies, Cronobacter dublinensis subsp. dublinensis subsp. nov., Cronobacter dublinensis subsp. lausannensis subsp. nov. and Cronobacter dublinensis subsp. lactaridi subsp. nov.	Iversen C, Mullane N, McCardell B, Tall BD, Lehner A, Fanning S, Stephan R, Joosten H	Int J Syst Evol Microbiol	10.1099/ijs.0.65577-0	2008	*

References

#7664

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 18703

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#32494

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #28714 (see below)

#60757 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 55852

#68368

Automatically annotated from API 20E .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#74729

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID354308.1 )

#28714

IJSEM 1442 2008 ( DOI 10.1099/ijs.0.65577-0 , PubMed 18523192 )

* These data were automatically processed and therefore are not curated

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