Name: Cronobacter dublinensis subsp. lactaridi E464
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 5240

Type strain:

Species: Cronobacter dublinensis subsp. lactaridi

Strain Designation: E464

Culture col. no.: DSM 18707, LMG 23825, CCUG 58094, JCM 16468

Strain history: LMG 23825 <-- A. Lehner E464 <-- C. Iversen.

NCBI tax ID(s): 1159496 (strain), 413499 (subspecies)

SI-ID 354305

DSM 18707, LMG 23825, JCM 16468, CCUG 58094

Section

Cronobacter dublinensis subsp. lactaridi E464 is an aerobe, mesophilic, motile bacterium that was isolated from milk powder production facility.

motile
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Enterobacterales*
	Family	*Enterobacteriaceae*
	Genus	*Cronobacter*
	Species	**Cronobacter dublinensis subsp. lactaridi**
	Full Scientific Name (LPSN)	Cronobacter dublinensis subsp. lactaridi Iversen et al. 2008

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	negative Confidence in %99.881

[Ref.: #69480]	Motility	yes Confidence in %96.168

Information on culture and growth conditions Culture and growth conditions

[Ref.: #7668]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

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Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

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Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

[Ref.: #7668]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #7668]	growth	37 °C
[Ref.: #61594]	growth	30 °C
[Ref.: #67770]	growth	30 °C

[Ref.: #7668]	Temperature range	mesophilic
[Ref.: #61594]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #61594]

Oxygen tolerance

aerobe

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.615

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68368]	amygdalin ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	arginine ChEBI	+	hydrolysis	* from API 20E, API ID32E
[Ref.: #68374]	cellobiose ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68368]	citrate ChEBI	+	assimilation	* from API 20E
[Ref.: #68374]	D-arabitol ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68374]	D-galacturonic acid ChEBI	+	builds acid from	* from API ID32E
[Ref.: #68374]	D-glucose ChEBI	+	builds acid from	* from API ID32E
[Ref.: #68368]	D-glucose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	D-mannitol ChEBI	+	fermentation	* from API 20E
[Ref.: #68374]	D-mannitol ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68368]	gelatin ChEBI	+	hydrolysis	* from API 20E
[Ref.: #68368]	L-arabinose ChEBI	+	fermentation	* from API 20E
[Ref.: #68374]	L-arabinose ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68374]	L-arabitol ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68368]	L-rhamnose ChEBI	+	fermentation	* from API 20E
[Ref.: #68374]	L-rhamnose ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68368]	lysine ChEBI	-	degradation	* from API 20E, API ID32E
[Ref.: #68374]	malonate ChEBI	-	assimilation	* from API ID32E
[Ref.: #68374]	maltose ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68368]	melibiose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	myo-inositol ChEBI	+	fermentation	* from API 20E
[Ref.: #68374]	myo-inositol ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68368]	ornithine ChEBI	+	degradation	* from API 20E, API ID32E
[Ref.: #68374]	palatinose ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68374]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68374]	ribitol ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68374]	sorbitol ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68368]	sorbitol ChEBI	-	fermentation	* from API 20E
[Ref.: #68374]	sucrose ChEBI	+	builds acid from	* from API ID32E
[Ref.: #68368]	sucrose ChEBI	+	fermentation	* from API 20E
[Ref.: #68374]	trehalose ChEBI	+	builds acid from	* from API ID32E
[Ref.: #68368]	tryptophan ChEBI	+	energy source	* from API 20E, API ID32E
[Ref.: #68368]	urea ChEBI	-	hydrolysis	* from API 20E, API ID32E
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #68368]	acetoin ChEBI	yes	* from API 20E
[Ref.: #68368]	hydrogen sulfide ChEBI	no	* from API 20E
[Ref.: #68374]	indole ChEBI	yes	* from API ID32E, API 20E

	Physiological tests	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #68368]		acetoin ChEBI	+		* from API 20E
[Ref.: #68374]		indole ChEBI		+	* from API ID32E, API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #68374]	alpha-galactosidase	+	3.2.1.22	* from API ID32E
[Ref.: #68374]	alpha-glucosidase	+	3.2.1.20	* from API ID32E
[Ref.: #68374]	alpha-maltosidase	+		* from API ID32E
[Ref.: #68374]	arginine dihydrolase	+	3.5.3.6	* from API ID32E, API 20E
[Ref.: #68374]	beta-galactosidase	+	3.2.1.23	* from API ID32E, API 20E
[Ref.: #68374]	beta-glucosidase	+	3.2.1.21	* from API ID32E
[Ref.: #68374]	beta-glucuronidase	-	3.2.1.31	* from API ID32E
[Ref.: #68368]	cytochrome oxidase	-	1.9.3.1	* from API 20E
[Ref.: #68368]	gelatinase	+		* from API 20E
[Ref.: #68374]	L-aspartate arylamidase	-	3.4.11.21	* from API ID32E
[Ref.: #68374]	lipase	+		* from API ID32E
[Ref.: #68374]	lysine decarboxylase	-	4.1.1.18	* from API ID32E, API 20E
[Ref.: #68374]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API ID32E
[Ref.: #68374]	ornithine decarboxylase	+	4.1.1.17	* from API ID32E, API 20E
[Ref.: #68368]	tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #68374]	urease	-	3.5.1.5	* from API ID32E, API 20E
	Only first 10 entries are displayed. Click here to see all.

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #7668]	18008	+	+	-	+	+	-	-	-	+	+	+	+	+	+	-	+	+	+	+	+	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API® ID32E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Ornithine decarboxylaseODC	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Urease/urea hydrolysisURE	Acid from L-arabitolLARL	Acid from D-galacturonic acidGAT	Acid from 5-Ketogluconate5KG	LipaseLIP	Phenol red (Acidification)RP	beta-Glucosidasebeta GLU	Acid from D-mannitolMAN	Acid from maltoseMAL	Acid from D-adonitolADO	Acid from palatinosePLE	beta-Glucuronidasebeta GUR	MalonateMNT	Indole productionIND	N-Acetyl-beta-Glucosaminidasebeta NAG	beta-Galactosidasebeta GAL	Acid from D-glucoseGLU	Acid from sucroseSAC	Acid from L-arabinoseLARA	Acid from D-arabitolDARL	alpha-Glucosidasealpha GLU	alpha-Galactosidasealpha GAL	Acid from trehaloseTRE	Acid from L-rhamnoseRHA	Acid from inositolINO	Acid from cellobioseCEL	Acid from sorbitolSOR	alpha-MaltosidasealphaMAL	L-aspartic acid arylamidaseAspA
[Ref.: #61594]	1872	+	+	-	-	-	+	-	+	-	+	-	-	-	-	-	-	+	+	+	+	+	-	-	+	+	+	-	-	-	-	+	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #7668]	Sample type/isolated from	milk powder production facility
[Ref.: #7668]	Country	Switzerland
[Ref.: #7668]	Country ISO 3 Code	CHE
[Ref.: #7668]	Continent	Europe
[Ref.: #7668]	Geographic location	46.8132°/8.2242°

[Ref.: #61594]	Sample type/isolated from	Milk powder,production facility
[Ref.: #61594]	Sampling date	2003
[Ref.: #61594]	Country	Switzerland
[Ref.: #61594]	Country ISO 3 Code	CHE
[Ref.: #61594]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Dried milk production facility
* marker position based on {}

Isolation sources categories

#Engineered	#Food production	#Dairy product
#Engineered	#Industrial	#Plant (Factory)
#Host Body Product	#Fluids	#Milk

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence EF059838 (>99% sequence identity) for Cronobacter from Microbeatlas

Aquatic Samples	2553
Soil Samples	1166
Animal Samples	20787
Plant Samples	2029
Total Samples	26535

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #7668]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Cronobacter dublinensis subsp. lactaridi LMG 23825 strain E464 16S ribosomal RNA gene, partial sequence	EF059838	1380	ENA	1159496 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Cronobacter dublinensis subsp. lactaridi LMG 23825	GCA_000409345	contig	GenBank	1159496 tax ID
[Ref.: #66792]	Cronobacter dublinensis subsp. lactaridi LMG 23825	1159496.3	wgs	PATRIC	1159496 tax ID	*
[Ref.: #66792]	Cronobacter dublinensis lactaridi LMG 23825	2548876989	draft	JGI IMG/M	1159496 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Cronobacter dublinensis subsp. lactaridi LMG 23825 PATRIC: 1159496.3
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Cronobacter dublinensis subsp. lactaridi LMG 23825 PATRIC: 1159496.3
[Ref.: #69480]	motile	yes	91.716	no
[Ref.: #69480]	gram-positive	no	97.828	no
[Ref.: #69480]	anaerobic	no	96.772	yes
[Ref.: #69480]	aerobic	yes	79.098	yes
[Ref.: #69480]	halophile	no	78.124	no
[Ref.: #69480]	spore-forming	no	94.033	no
[Ref.: #69480]	flagellated	no	79.286	no
[Ref.: #69480]	thermophile	no	99.242	yes
[Ref.: #69480]	glucose-util	yes	95.777	no
[Ref.: #69480]	glucose-ferment	yes	93.053	no

Availability in culture collections External links

[Ref.: #7668]

Culture collection no.

DSM 18707, LMG 23825, CCUG 58094, JCM 16468

[Ref.: #74731]

SI-ID 354305

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Cronobacter gen. nov., a new genus to accommodate the biogroups of Enterobacter sakazakii, and proposal of Cronobacter sakazakii gen. nov., comb. nov., Cronobacter malonaticus sp. nov., Cronobacter turicensis sp. nov., Cronobacter muytjensii sp. nov., Cronobacter dublinensis sp. nov., Cronobacter genomospecies 1, and of three subspecies, Cronobacter dublinensis subsp. dublinensis subsp. nov., Cronobacter dublinensis subsp. lausannensis subsp. nov. and Cronobacter dublinensis subsp. lactaridi subsp. nov.	Iversen C, Mullane N, McCardell B, Tall BD, Lehner A, Fanning S, Stephan R, Joosten H	Int J Syst Evol Microbiol	10.1099/ijs.0.65577-0	2008	*

References

#7668

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 18707

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#61594 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 58094

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68368

Automatically annotated from API 20E .

#68374

Automatically annotated from API ID32E .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#74731

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID354305.1 )

* These data were automatically processed and therefore are not curated

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Cronobacter dublinensis subsp. lactaridi LMG 23825

Cronobacter dublinensis subsp. lactaridi LMG 23825

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