Name: Corynebacterium doosanense DSM 45436
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 3235

Type strain:

Species: Corynebacterium doosanense

Culture col. no.: DSM 45436, CCUG 57284, KCTC 19568, JCM 17317, CAU 212

Strain history: <- WY Kim, Chung-Ang Univ.

NCBI tax ID(s): 558173 (strain), 1121358 (species)

SI-ID 364660

KCTC 19568, CCUG 57284, JCM 17317, DSM 45436

Special Collections

Section

Corynebacterium doosanense DSM 45436 is an aerobe, Gram-positive, rod-shaped bacterium that was isolated from activated sludge taken from a wastewater treatment plant.

Gram-positive
rod-shaped
aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Mycobacteriales*
	Family	*Corynebacteriaceae*
	Genus	*Corynebacterium*
	Species	**Corynebacterium doosanense**
	Full Scientific Name (LPSN)	Corynebacterium doosanense Lee et al. 2009

Information on morphological and physiological properties Morphology

[Ref.: #29279]	Gram stain	positive
[Ref.: #67771]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %93.661

[Ref.: #29279]	Cell length	1.1 µm

[Ref.: #29279]	Cell width	0.9 µm

[Ref.: #29279]	Cell shape	rod-shaped

[Ref.: #29279]	Motility	no
[Ref.: #69480]	Motility	no Confidence in %92.5

[Ref.: #61273]

Incubation period

2 days

[Ref.: #29279]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #16814]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #16814]

Culture medium growth

[Ref.: #16814]

Culture medium link

Medium recipe at MediaDive

[Ref.: #16814]

Culture medium composition

expand / minimize

[Ref.: #16814]

Culture medium

TRYPTICASE SOY BROTH AGAR+BLOOD (DSMZ Medium 535a)

[Ref.: #16814]

Culture medium growth

[Ref.: #16814]

Culture medium link

Medium recipe at MediaDive

[Ref.: #16814]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #16814]	growth	28 °C
[Ref.: #61273]	growth	30 °C
[Ref.: #67770]	growth	28 °C
[Ref.: #67771]	growth	30 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #29279]	Oxygen tolerance	aerobe
[Ref.: #61273]	Oxygen tolerance	aerobe
[Ref.: #67771]	Oxygen tolerance	aerobe

[Ref.: #29279]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %99

	Metabolite utilization	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #29279]		glucose ChEBI	+	carbon source
[Ref.: #29279]		nitrate ChEBI	+	reduction

	Enzyme	Enzyme activity	EC number
[Ref.: #29279]	catalase	+	1.11.1.6
[Ref.: #29279]	cytochrome oxidase	+	1.9.3.1
[Ref.: #29279]	pyrazinamidase	+	3.5.1.B15

	Pathway	Enzyme coverage	Annotated reactions
	Pathway annotation	Computationally determined by
[Ref.: #66794]	coenzyme A metabolism	100	4 of 4
[Ref.: #66794]	lipoate biosynthesis	100	5 of 5
[Ref.: #66794]	phenylmercury acetate degradation	100	2 of 2
[Ref.: #66794]	adipate degradation	100	2 of 2
[Ref.: #66794]	formaldehyde oxidation	100	3 of 3
[Ref.: #66794]	ppGpp biosynthesis	100	4 of 4
[Ref.: #66794]	cyanate degradation	100	3 of 3
[Ref.: #66794]	vitamin K metabolism	100	5 of 5
[Ref.: #66794]	cis-vaccenate biosynthesis	100	2 of 2
[Ref.: #66794]	denitrification	100	2 of 2
[Ref.: #66794]	ceramide biosynthesis	100	1 of 1
[Ref.: #66794]	palmitate biosynthesis	100	22 of 22
[Ref.: #66794]	acetate fermentation	100	4 of 4
[Ref.: #66794]	CDP-diacylglycerol biosynthesis	100	2 of 2
[Ref.: #66794]	UDP-GlcNAc biosynthesis	100	3 of 3
[Ref.: #66794]	suberin monomers biosynthesis	100	2 of 2
[Ref.: #66794]	anapleurotic synthesis of oxalacetate	100	1 of 1
[Ref.: #66794]	folate polyglutamylation	100	1 of 1
[Ref.: #66794]	starch degradation	90	9 of 10
[Ref.: #66794]	threonine metabolism	90	9 of 10
[Ref.: #66794]	aspartate and asparagine metabolism	88.89	8 of 9
[Ref.: #66794]	chorismate metabolism	88.89	8 of 9
[Ref.: #66794]	isoleucine metabolism	87.5	7 of 8
[Ref.: #66794]	photosynthesis	85.71	12 of 14
[Ref.: #66794]	propanol degradation	85.71	6 of 7
[Ref.: #66794]	glycolate and glyoxylate degradation	83.33	5 of 6
[Ref.: #66794]	pentose phosphate pathway	81.82	9 of 11
[Ref.: #66794]	propionate fermentation	80	8 of 10
[Ref.: #66794]	peptidoglycan biosynthesis	80	12 of 15
[Ref.: #66794]	flavin biosynthesis	80	12 of 15
[Ref.: #66794]	citric acid cycle	78.57	11 of 14
[Ref.: #66794]	heme metabolism	78.57	11 of 14
[Ref.: #66794]	molybdenum cofactor biosynthesis	77.78	7 of 9
[Ref.: #66794]	serine metabolism	77.78	7 of 9
[Ref.: #66794]	valine metabolism	77.78	7 of 9
[Ref.: #66794]	phenylalanine metabolism	76.92	10 of 13
[Ref.: #66794]	gluconeogenesis	75	6 of 8
[Ref.: #66794]	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
[Ref.: #66794]	butanoate fermentation	75	3 of 4
[Ref.: #66794]	glycogen biosynthesis	75	3 of 4
[Ref.: #66794]	sulfopterin metabolism	75	3 of 4
[Ref.: #66794]	C4 and CAM-carbon fixation	75	6 of 8
[Ref.: #66794]	ketogluconate metabolism	75	6 of 8
[Ref.: #66794]	metabolism of disaccharids	72.73	8 of 11
[Ref.: #66794]	alanine metabolism	72.41	21 of 29
[Ref.: #66794]	reductive acetyl coenzyme A pathway	71.43	5 of 7
[Ref.: #66794]	glutamate and glutamine metabolism	71.43	20 of 28
[Ref.: #66794]	cardiolipin biosynthesis	71.43	5 of 7
[Ref.: #66794]	glycolysis	70.59	12 of 17
[Ref.: #66794]	purine metabolism	70.21	66 of 94
[Ref.: #66794]	isoprenoid biosynthesis	69.23	18 of 26
[Ref.: #66794]	vitamin B1 metabolism	69.23	9 of 13
[Ref.: #66794]	CO2 fixation in Crenarchaeota	66.67	6 of 9
[Ref.: #66794]	selenocysteine biosynthesis	66.67	4 of 6
[Ref.: #66794]	4-hydroxymandelate degradation	66.67	6 of 9
[Ref.: #66794]	acetoin degradation	66.67	2 of 3
[Ref.: #66794]	L-lactaldehyde degradation	66.67	2 of 3
[Ref.: #66794]	octane oxidation	66.67	2 of 3
[Ref.: #66794]	oxidative phosphorylation	65.93	60 of 91
[Ref.: #66794]	histidine metabolism	65.52	19 of 29
[Ref.: #66794]	lipid metabolism	64.52	20 of 31
[Ref.: #66794]	tetrahydrofolate metabolism	64.29	9 of 14
[Ref.: #66794]	glutathione metabolism	64.29	9 of 14
[Ref.: #66794]	proline metabolism	63.64	7 of 11
[Ref.: #66794]	pyrimidine metabolism	62.22	28 of 45
[Ref.: #66794]	methionine metabolism	61.54	16 of 26
[Ref.: #66794]	leucine metabolism	61.54	8 of 13
[Ref.: #66794]	NAD metabolism	61.11	11 of 18
[Ref.: #66794]	methylglyoxal degradation	60	3 of 5
[Ref.: #66794]	glycine betaine biosynthesis	60	3 of 5
[Ref.: #66794]	glycogen metabolism	60	3 of 5
[Ref.: #66794]	non-pathway related	57.89	22 of 38
[Ref.: #66794]	ubiquinone biosynthesis	57.14	4 of 7
[Ref.: #66794]	d-mannose degradation	55.56	5 of 9
[Ref.: #66794]	nitrate assimilation	55.56	5 of 9
[Ref.: #66794]	cysteine metabolism	55.56	10 of 18
[Ref.: #66794]	tryptophan metabolism	55.26	21 of 38
[Ref.: #66794]	arginine metabolism	54.17	13 of 24
[Ref.: #66794]	urea cycle	53.85	7 of 13
[Ref.: #66794]	dTDPLrhamnose biosynthesis	50	4 of 8
[Ref.: #66794]	CMP-KDO biosynthesis	50	2 of 4
[Ref.: #66794]	cyclohexanol degradation	50	2 of 4
[Ref.: #66794]	lysine metabolism	50	21 of 42
[Ref.: #66794]	mannosylglycerate biosynthesis	50	1 of 2
[Ref.: #66794]	ethanol fermentation	50	1 of 2
[Ref.: #66794]	lactate fermentation	50	2 of 4
[Ref.: #66794]	sulfate reduction	46.15	6 of 13
[Ref.: #66794]	vitamin B6 metabolism	45.45	5 of 11
[Ref.: #66794]	phenol degradation	45	9 of 20
[Ref.: #66794]	degradation of aromatic, nitrogen containing compounds	41.67	5 of 12
[Ref.: #66794]	arachidonate biosynthesis	40	2 of 5
[Ref.: #66794]	gallate degradation	40	2 of 5
[Ref.: #66794]	glycine metabolism	40	4 of 10
[Ref.: #66794]	phenylpropanoid biosynthesis	38.46	5 of 13
[Ref.: #66794]	cholesterol biosynthesis	36.36	4 of 11
[Ref.: #66794]	tyrosine metabolism	35.71	5 of 14
[Ref.: #66794]	methane metabolism	33.33	1 of 3
[Ref.: #66794]	acetyl CoA biosynthesis	33.33	1 of 3
[Ref.: #66794]	IAA biosynthesis	33.33	1 of 3
[Ref.: #66794]	enterobactin biosynthesis	33.33	1 of 3
[Ref.: #66794]	(5R)-carbapenem carboxylate biosynthesis	33.33	1 of 3
[Ref.: #66794]	3-phenylpropionate degradation	33.33	5 of 15
[Ref.: #66794]	lipid A biosynthesis	33.33	3 of 9
[Ref.: #66794]	degradation of sugar alcohols	31.25	5 of 16
[Ref.: #66794]	coenzyme M biosynthesis	30	3 of 10
[Ref.: #66794]	Entner Doudoroff pathway	30	3 of 10
[Ref.: #66794]	benzoyl-CoA degradation	28.57	2 of 7
[Ref.: #66794]	degradation of sugar acids	28	7 of 25
[Ref.: #66794]	degradation of hexoses	27.78	5 of 18
[Ref.: #66794]	chlorophyll metabolism	27.78	5 of 18
[Ref.: #66794]	dolichyl-diphosphooligosaccharide biosynthesis	27.27	3 of 11
[Ref.: #66794]	ascorbate metabolism	27.27	6 of 22
[Ref.: #66794]	toluene degradation	25	1 of 4
[Ref.: #66794]	degradation of pentoses	25	7 of 28
[Ref.: #66794]	biotin biosynthesis	25	1 of 4
[Ref.: #66794]	carnitine metabolism	25	2 of 8
[Ref.: #66794]	vitamin B12 metabolism	23.53	8 of 34
		Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #16814]	Sample type/isolated from	activated sludge taken from a wastewater treatment plant
[Ref.: #16814]	Geographic location (country and/or sea, region)	Yeongdeuk-gun
[Ref.: #16814]	Country	Republic of Korea
[Ref.: #16814]	Country ISO 3 Code	KOR
[Ref.: #16814]	Continent	Asia

[Ref.: #61273]	Sample type/isolated from	Activated sludge
[Ref.: #61273]	Sampling date	2007-03-01
[Ref.: #61273]	Geographic location (country and/or sea, region)	Yeongdeuk-gun
[Ref.: #61273]	Country	Republic of Korea
[Ref.: #61273]	Country ISO 3 Code	KOR
[Ref.: #61273]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Activated sludge from a wastewater treatment plant
[Ref.: #67770]	Geographic location (country and/or sea, region)	Yeongdeuk-gun
[Ref.: #67770]	Country	Republic of Korea
[Ref.: #67770]	Country ISO 3 Code	KOR
[Ref.: #67770]	Continent	Asia

[Ref.: #67771]	Sample type/isolated from	From waste water
[Ref.: #67771]	Geographic location (country and/or sea, region)	Yeongdeok-Gun
[Ref.: #67771]	Country	Republic of Korea
[Ref.: #67771]	Country ISO 3 Code	KOR
[Ref.: #67771]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Engineered	#Waste	#Activated sludge
#Engineered	#Waste	#Water treatment plant

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence EU998655 (>99% sequence identity) for Corynebacterium doosanense subclade from Microbeatlas

Aquatic Samples	142
Soil Samples	98
Animal Samples	1841
Plant Samples	66
Total Samples	2147

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #16814]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #16814]	Corynebacterium sp. CAU 212 16S ribosomal RNA gene, partial sequence	EU998655	1494	GenBank ENA	558173 tax ID

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Corynebacterium doosanense CAU 212 = DSM 45436	GCA_000372245	contig	GenBank ENA	558173 tax ID
[Ref.: #66792]	Corynebacterium doosanense CAU 212 = DSM 45436 CAU 212(T)	GCA_000767055	complete	GenBank ENA	558173 tax ID	*
[Ref.: #66792]	Corynebacterium doosanense CAU 212 = DSM 45436	558173.4	complete	PATRIC	558173 tax ID	*
[Ref.: #66792]	Corynebacterium doosanense CAU 212 = DSM 45436 strain CAU 212(T)	558173.7	plasmid	PATRIC	558173 tax ID	*
[Ref.: #66792]	Corynebacterium doosanense DSM 45436	558173.3	wgs	PATRIC	558173 tax ID	*
[Ref.: #66792]	Corynebacterium doosanense CAU 212, DSM 45436	2639762811	complete	JGI IMG/M	558173 tax ID	*

[Ref.: #16814]	GC-content	53.5 mol%
[Ref.: #67770]	GC-content	53.5 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #67770]	GC-content	66.9 mol%	genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Corynebacterium doosanense CAU 212 = DSM 45436 CAU 212 NCBI: GCA_000767055.1
[Ref.: #69481]	spore-forming	spore-formingⓘ	no	99	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Corynebacterium doosanense CAU 212 = DSM 45436 CAU 212 NCBI: GCA_000767055.1
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	93.661	yes
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	94.949	yes
[Ref.: #69480]	aerobic	aerobicⓘ	yes	71.017	yes
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	80.512	yes
[Ref.: #69480]	thermophilic	thermophileⓘ	no	93.5	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	no	92.5	yes

Availability in culture collections External links

[Ref.: #16814]

Culture collection no.

DSM 45436, CCUG 57284, KCTC 19568, JCM 17317, CAU 212

[Ref.: #72749]

SI-ID 364660

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Corynebacterium doosanense sp. nov., isolated from activated sludge.	Lee HJ, Cho SL, Jung MY, Van Nguyen TH, Jung YC, Park HK, Le VP, Kim W	Int J Syst Evol Microbiol	10.1099/ijs.0.010108-0	2009	*

References

#16814

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 45436

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#29279

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #25695 (see below)

#61273 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 57284

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#66794

Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#72749

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID364660.1 )

#25695

IJSEM 2734 2009 ( DOI 10.1099/ijs.0.010108-0 , PubMed 19625432 )

* These data were automatically processed and therefore are not curated

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Corynebacterium doosanense CAU 212 = DSM 45436 CAU 212

Corynebacterium doosanense CAU 212 = DSM 45436 CAU 212

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