Strain identifier

BacDive ID: 3235

Type strain: Yes

Species: Corynebacterium doosanense

Strain history: <- WY Kim, Chung-Ang Univ.

NCBI tax ID(s): 558173 (strain), 1121358 (species)

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General

@ref: 16814

BacDive-ID: 3235

DSM-Number: 45436

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive, rod-shaped

description: Corynebacterium doosanense DSM 45436 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from activated sludge taken from a wastewater treatment plant.

NCBI tax id

NCBI tax idMatching level
1121358species
558173strain

strain history

@refhistory
16814<- CCUG; CCUG 57284
67770CCUG 57284 <-- W. Kim CAU 212.
67771<- WY Kim, Chung-Ang Univ.

doi: 10.13145/bacdive3235.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Corynebacteriaceae
  • genus: Corynebacterium
  • species: Corynebacterium doosanense
  • full scientific name: Corynebacterium doosanense Lee et al. 2009

@ref: 16814

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Corynebacteriaceae

genus: Corynebacterium

species: Corynebacterium doosanense

full scientific name: Corynebacterium doosanense Lee et al. 2009 emend. Nouioui et al. 2018

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
29279positive1.1 µm0.9 µmrod-shapedno
67771positive
69480no93.144
69480positive100

colony morphology

  • @ref: 61273
  • incubation period: 2 days

pigmentation

  • @ref: 29279
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
16814COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
16814TRYPTICASE SOY BROTH AGAR+BLOOD (DSMZ Medium 535a)yeshttps://mediadive.dsmz.de/medium/535aName: TRYPTICASE SOY BROTH AGAR+BLOOD (DSMZ Medium 535a) Composition: None 50.0 g/l Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
16814positivegrowth28mesophilic
61273positivegrowth30mesophilic
67770positivegrowth28mesophilic
67771positivegrowth30mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
29279aerobe
61273aerobe
67771aerobe

spore formation

@refspore formationconfidence
29279no
69481no99
69480no99.975

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2927917234glucose+carbon source
2927917632nitrate+reduction

enzymes

@refvalueactivityec
29279catalase+1.11.1.6
29279cytochrome oxidase+1.9.3.1
29279pyrazinamidase+3.5.1.B15

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling date
16814activated sludge taken from a wastewater treatment plantYeongdeuk-gunRepublic of KoreaKORAsia
61273Activated sludgeYeongdeuk-gunRepublic of KoreaKORAsia2007-03-01
67770Activated sludge from a wastewater treatment plantYeongdeuk-gunRepublic of KoreaKORAsia
67771From waste waterYeongdeok-GunRepublic of KoreaKORAsia

isolation source categories

Cat1Cat2Cat3
#Engineered#Waste#Activated sludge
#Engineered#Waste#Water treatment plant

taxonmaps

  • @ref: 69479
  • File name: preview.99_2543.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_10;96_1368;97_1625;98_1976;99_2543&stattab=map
  • Last taxonomy: Corynebacterium doosanense subclade
  • 16S sequence: EU998655
  • Sequence Identity:
  • Total samples: 2147
  • soil counts: 98
  • aquatic counts: 142
  • animal counts: 1841
  • plant counts: 66

Safety information

risk assessment

  • @ref: 16814
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 16814
  • description: Corynebacterium sp. CAU 212 16S ribosomal RNA gene, partial sequence
  • accession: EU998655
  • length: 1494
  • database: ena
  • NCBI tax ID: 558173

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Corynebacterium doosanense CAU 212 = DSM 45436 CAU 212(T)GCA_000767055completencbi558173
66792Corynebacterium doosanense CAU 212 = DSM 45436558173.4completepatric558173
66792Corynebacterium doosanense CAU 212 = DSM 45436 strain CAU 212(T)558173.7plasmidpatric558173
66792Corynebacterium doosanense DSM 45436558173.3wgspatric558173
66792Corynebacterium doosanense CAU 212, DSM 454362639762811completeimg558173
66792Corynebacterium doosanense CAU 212, DSM 454362515154029draftimg558173
67770Corynebacterium doosanense CAU 212 = DSM 45436GCA_000372245contigncbi558173

GC content

@refGC-contentmethod
1681453.5
6777053.5thermal denaturation, midpoint method (Tm)
6777066.9genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno99no
motileno94.094yes
flagellatedno97.844yes
gram-positiveyes94.428yes
anaerobicno99.32no
aerobicyes69.714yes
halophileyes69.524no
spore-formingno95.329no
thermophileno96.983yes
glucose-utilyes90.167yes
glucose-fermentno65.543no

External links

@ref: 16814

culture collection no.: DSM 45436, CCUG 57284, KCTC 19568, JCM 17317, CAU 212

straininfo link

  • @ref: 72749
  • straininfo: 364660

literature

  • topic: Phylogeny
  • Pubmed-ID: 19625432
  • title: Corynebacterium doosanense sp. nov., isolated from activated sludge.
  • authors: Lee HJ, Cho SL, Jung MY, Van Nguyen TH, Jung YC, Park HK, Le VP, Kim W
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.010108-0
  • year: 2009
  • mesh: Corynebacterium/classification/genetics/*isolation & purification, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Sewage/*microbiology
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmedjournal
16814Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 45436)https://www.dsmz.de/collection/catalogue/details/culture/DSM-45436
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
29279Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2569528776041
61273Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 57284)https://www.ccug.se/strain?id=57284
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updates33211880Nucleic Acids Res. 49: D498-D508 2020
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
72749Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID364660.1StrainInfo: A central database for resolving microbial strain identifiers