Name: Christensenella minuta DSM 22607
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 24747

Type strain:

Species: Christensenella minuta

Culture col. no.: DSM 22607, JCM 16072, YIT 12065, KCTC 15498

Strain history: Y. Watanabe A8 (=YIT 12065).

NCBI tax ID(s): 626937 (species)

SI-ID 403729

DSM 22607

Section

Christensenella minuta DSM 22607 is an anaerobe, mesophilic, Gram-negative bacterium that was isolated from faeces from a healthy Japanese male.

Gram-negative
rod-shaped
anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Clostridia*
	Order	*Eubacteriales*
	Family	*Christensenellaceae*
	Genus	*Christensenella*
	Species	**Christensenella minuta**
	Full Scientific Name (LPSN)	Christensenella minuta Morotomi et al. 2012

Information on morphological and physiological properties Morphology

[Ref.: #29932]	Gram stain	negative
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #29932]	Cell length	0.8-1.9 µm

[Ref.: #29932]	Cell width	0.4 µm

[Ref.: #29932]	Cell shape	rod-shaped

[Ref.: #29932]	Motility	no

[Ref.: #29932]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #21228]

Culture medium

WILKINS-CHALGREN ANAEROBE BROTH (N2/CO2) (DSMZ Medium 339a)

[Ref.: #21228]

Culture medium growth

[Ref.: #21228]

Culture medium link

Medium recipe at MediaDive

[Ref.: #21228]

Culture medium composition

expand / minimize

[Ref.: #21228]

Culture medium

RCM MEDIUM (N2/CO2) (DSMZ Medium 634c)

[Ref.: #21228]

Culture medium growth

[Ref.: #21228]

Culture medium link

Medium recipe at MediaDive

[Ref.: #21228]

Culture medium composition

expand / minimize

[Ref.: #21228]

Culture medium

CHOPPED MEDIUM (N2/CO2) (DSMZ Medium 78b)

[Ref.: #21228]

Culture medium growth

[Ref.: #21228]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #21228]	growth	37 °C
[Ref.: #29932]	growth	25-43 °C
[Ref.: #29932]	optimum	37 °C
[Ref.: #67770]	growth	37 °C

[Ref.: #21228]	Temperature range	mesophilic
[Ref.: #29932]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #29932]			optimum	7.5
[Ref.: #29932]			growth	6.0-9.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #21228]	Oxygen tolerance	anaerobe
[Ref.: #29932]	Oxygen tolerance	anaerobe
[Ref.: #69480]	Oxygen tolerance	anaerobe Confidence in %99.308

[Ref.: #29932]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %94
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.971

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #29932]		NaCl		growth	<5	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #29932]	arabinose ChEBI	+	carbon source
[Ref.: #29932]	glucose ChEBI	+	carbon source
[Ref.: #29932]	mannose ChEBI	+	carbon source
[Ref.: #29932]	rhamnose ChEBI	+	carbon source
[Ref.: #29932]	salicin ChEBI	+	carbon source
[Ref.: #29932]	xylose ChEBI	+	carbon source

	Pathway	Enzyme coverage	Annotated reactions
	Pathway annotation	Computationally determined by
[Ref.: #66794]	adipate degradation	100	2 of 2
[Ref.: #66794]	gluconeogenesis	100	8 of 8
[Ref.: #66794]	L-lactaldehyde degradation	100	3 of 3
[Ref.: #66794]	ribulose monophosphate pathway	100	2 of 2
[Ref.: #66794]	starch degradation	100	10 of 10
[Ref.: #66794]	palmitate biosynthesis	100	22 of 22
[Ref.: #66794]	coenzyme A metabolism	100	4 of 4
[Ref.: #66794]	CDP-diacylglycerol biosynthesis	100	2 of 2
[Ref.: #66794]	UDP-GlcNAc biosynthesis	100	3 of 3
[Ref.: #66794]	formaldehyde oxidation	100	3 of 3
[Ref.: #66794]	folate polyglutamylation	100	1 of 1
[Ref.: #66794]	suberin monomers biosynthesis	100	2 of 2
[Ref.: #66794]	cis-vaccenate biosynthesis	100	2 of 2
[Ref.: #66794]	anapleurotic synthesis of oxalacetate	100	1 of 1
[Ref.: #66794]	C4 and CAM-carbon fixation	100	8 of 8
[Ref.: #66794]	pentose phosphate pathway	90.91	10 of 11
[Ref.: #66794]	chorismate metabolism	88.89	8 of 9
[Ref.: #66794]	valine metabolism	88.89	8 of 9
[Ref.: #66794]	degradation of sugar alcohols	87.5	14 of 16
[Ref.: #66794]	reductive acetyl coenzyme A pathway	85.71	6 of 7
[Ref.: #66794]	propanol degradation	85.71	6 of 7
[Ref.: #66794]	phenylalanine metabolism	84.62	11 of 13
[Ref.: #66794]	glutamate and glutamine metabolism	82.14	23 of 28
[Ref.: #66794]	Entner Doudoroff pathway	80	8 of 10
[Ref.: #66794]	peptidoglycan biosynthesis	80	12 of 15
[Ref.: #66794]	threonine metabolism	80	8 of 10
[Ref.: #66794]	photosynthesis	78.57	11 of 14
[Ref.: #66794]	CO2 fixation in Crenarchaeota	77.78	7 of 9
[Ref.: #66794]	d-mannose degradation	77.78	7 of 9
[Ref.: #66794]	ketogluconate metabolism	75	6 of 8
[Ref.: #66794]	ppGpp biosynthesis	75	3 of 4
[Ref.: #66794]	isoleucine metabolism	75	6 of 8
[Ref.: #66794]	glycogen biosynthesis	75	3 of 4
[Ref.: #66794]	CMP-KDO biosynthesis	75	3 of 4
[Ref.: #66794]	butanoate fermentation	75	3 of 4
[Ref.: #66794]	purine metabolism	73.4	69 of 94
[Ref.: #66794]	citric acid cycle	71.43	10 of 14
[Ref.: #66794]	degradation of pentoses	71.43	20 of 28
[Ref.: #66794]	glycolysis	70.59	12 of 17
[Ref.: #66794]	alanine metabolism	68.97	20 of 29
[Ref.: #66794]	methane metabolism	66.67	2 of 3
[Ref.: #66794]	serine metabolism	66.67	6 of 9
[Ref.: #66794]	cyanate degradation	66.67	2 of 3
[Ref.: #66794]	degradation of hexoses	66.67	12 of 18
[Ref.: #66794]	aspartate and asparagine metabolism	66.67	6 of 9
[Ref.: #66794]	octane oxidation	66.67	2 of 3
[Ref.: #66794]	acetoin degradation	66.67	2 of 3
[Ref.: #66794]	NAD metabolism	66.67	12 of 18
[Ref.: #66794]	methionine metabolism	65.38	17 of 26
[Ref.: #66794]	pyrimidine metabolism	64.44	29 of 45
[Ref.: #66794]	tetrahydrofolate metabolism	64.29	9 of 14
[Ref.: #66794]	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	62.5	5 of 8
[Ref.: #66794]	dTDPLrhamnose biosynthesis	62.5	5 of 8
[Ref.: #66794]	glycogen metabolism	60	3 of 5
[Ref.: #66794]	4-hydroxyphenylacetate degradation	60	6 of 10
[Ref.: #66794]	cellulose degradation	60	3 of 5
[Ref.: #66794]	hydrogen production	60	3 of 5
[Ref.: #66794]	propionate fermentation	60	6 of 10
[Ref.: #66794]	glycine betaine biosynthesis	60	3 of 5
[Ref.: #66794]	lipoate biosynthesis	60	3 of 5
[Ref.: #66794]	tryptophan metabolism	57.89	22 of 38
[Ref.: #66794]	oxidative phosphorylation	57.14	52 of 91
[Ref.: #66794]	cysteine metabolism	55.56	10 of 18
[Ref.: #66794]	non-pathway related	55.26	21 of 38
[Ref.: #66794]	urea cycle	53.85	7 of 13
[Ref.: #66794]	leucine metabolism	53.85	7 of 13
[Ref.: #66794]	histidine metabolism	51.72	15 of 29
[Ref.: #66794]	arginine metabolism	50	12 of 24
[Ref.: #66794]	glycolate and glyoxylate degradation	50	3 of 6
[Ref.: #66794]	toluene degradation	50	2 of 4
[Ref.: #66794]	kanosamine biosynthesis II	50	1 of 2
[Ref.: #66794]	sulfopterin metabolism	50	2 of 4
[Ref.: #66794]	ethanol fermentation	50	1 of 2
[Ref.: #66794]	glycine metabolism	50	5 of 10
[Ref.: #66794]	phenylmercury acetate degradation	50	1 of 2
[Ref.: #66794]	acetate fermentation	50	2 of 4
[Ref.: #66794]	selenocysteine biosynthesis	50	3 of 6
[Ref.: #66794]	lysine metabolism	50	21 of 42
[Ref.: #66794]	metabolism of disaccharids	45.45	5 of 11
[Ref.: #66794]	proline metabolism	45.45	5 of 11
[Ref.: #66794]	tyrosine metabolism	42.86	6 of 14
[Ref.: #66794]	cardiolipin biosynthesis	42.86	3 of 7
[Ref.: #66794]	ubiquinone biosynthesis	42.86	3 of 7
[Ref.: #66794]	isoprenoid biosynthesis	42.31	11 of 26
[Ref.: #66794]	degradation of aromatic, nitrogen containing compounds	41.67	5 of 12
[Ref.: #66794]	ascorbate metabolism	40.91	9 of 22
[Ref.: #66794]	methylglyoxal degradation	40	2 of 5
[Ref.: #66794]	metabolism of amino sugars and derivatives	40	2 of 5
[Ref.: #66794]	bacilysin biosynthesis	40	2 of 5
[Ref.: #66794]	factor 420 biosynthesis	40	2 of 5
[Ref.: #66794]	flavin biosynthesis	40	6 of 15
[Ref.: #66794]	arachidonate biosynthesis	40	2 of 5
[Ref.: #66794]	myo-inositol biosynthesis	40	4 of 10
[Ref.: #66794]	lipid metabolism	38.71	12 of 31
[Ref.: #66794]	vitamin B1 metabolism	38.46	5 of 13
[Ref.: #66794]	d-xylose degradation	36.36	4 of 11
[Ref.: #66794]	degradation of sugar acids	36	9 of 25
[Ref.: #66794]	polyamine pathway	34.78	8 of 23
[Ref.: #66794]	lipid A biosynthesis	33.33	3 of 9
[Ref.: #66794]	IAA biosynthesis	33.33	1 of 3
[Ref.: #66794]	sphingosine metabolism	33.33	2 of 6
[Ref.: #66794]	acetyl CoA biosynthesis	33.33	1 of 3
[Ref.: #66794]	molybdenum cofactor biosynthesis	33.33	3 of 9
[Ref.: #66794]	nitrate assimilation	33.33	3 of 9
[Ref.: #66794]	androgen and estrogen metabolism	31.25	5 of 16
[Ref.: #66794]	coenzyme M biosynthesis	30	3 of 10
[Ref.: #66794]	vitamin B12 metabolism	29.41	10 of 34
[Ref.: #66794]	benzoyl-CoA degradation	28.57	2 of 7
[Ref.: #66794]	lactate fermentation	25	1 of 4
[Ref.: #66794]	cyclohexanol degradation	25	1 of 4
[Ref.: #66794]	carnitine metabolism	25	2 of 8
[Ref.: #66794]	biotin biosynthesis	25	1 of 4
[Ref.: #66794]	phenol degradation	25	5 of 20
[Ref.: #66794]	sulfate reduction	23.08	3 of 13
[Ref.: #66794]	phosphatidylethanolamine bioynthesis	23.08	3 of 13
[Ref.: #66794]	arachidonic acid metabolism	22.22	4 of 18
[Ref.: #66794]	4-hydroxymandelate degradation	22.22	2 of 9
[Ref.: #66794]	glutathione metabolism	21.43	3 of 14
		Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #21228]	Sample type/isolated from	faeces from a healthy Japanese male
[Ref.: #21228]	Geographic location (country and/or sea, region)	Tokyo
[Ref.: #21228]	Country	Japan
[Ref.: #21228]	Country ISO 3 Code	JPN
[Ref.: #21228]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Human feces
* marker position based on {}

Isolation sources categories

#Host	#Human	#Male
#Host Body Product	#Gastrointestinal tract	#Feces (Stool)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AB490809 (>99% sequence identity) for Christensenella from Microbeatlas

Aquatic Samples	276
Soil Samples	159
Animal Samples	17409
Plant Samples	47
Total Samples	17891

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #21228]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #21228]	Christensenella minuta gene for 16S rRNA, partial sequence	AB490809	1497	ENA	626937 tax ID

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Christensenella minuta DSM 22607	GCA_001571425	scaffold	GenBank	626937 tax ID
[Ref.: #67770]	Christensenella minuta DSM 22607	GCA_001652705	contig	GenBank	626937 tax ID
[Ref.: #67770]	Christensenella minuta DSM 22607	GCA_001678855	scaffold	GenBank	626937 tax ID
[Ref.: #66792]	Christensenella minuta strain DSM 22607	626937.8	wgs	PATRIC	626937 tax ID	*
[Ref.: #66792]	Christensenella minuta strain DSM 22607	626937.30	wgs	PATRIC	626937 tax ID	*
[Ref.: #66792]	Christensenella minuta DSM 22607	2728369339	draft	JGI IMG/M	626937 tax ID	*

[Ref.: #21228]	GC-content	51.3 mol%	high performance liquid chromatography (HPLC)
[Ref.: #29932]	GC-content	51.3 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Christensenella minuta DSM 22607 NCBI: GCA_003628755.1
[Ref.: #69481]	spore-forming	no	94	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Christensenella minuta DSM 22607 NCBI: GCA_003628755.1
[Ref.: #69480]	motile	no	88.779	no
[Ref.: #69480]	flagellated	no	97.876	yes
[Ref.: #69480]	gram-positive	yes	81.757	no
[Ref.: #69480]	anaerobic	yes	98.613	yes
[Ref.: #69480]	aerobic	no	97.735	no
[Ref.: #69480]	halophile	no	93.425	no
[Ref.: #69480]	spore-forming	no	75.806	no
[Ref.: #69480]	glucose-util	yes	91.6	yes
[Ref.: #69480]	thermophile	no	90.771	yes
[Ref.: #69480]	glucose-ferment	yes	54.255	no

Availability in culture collections External links

[Ref.: #21228]

Culture collection no.

DSM 22607, JCM 16072, YIT 12065, KCTC 15498

[Ref.: #88575]

SI-ID 403729

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Description of Christensenella minuta gen. nov., sp. nov., isolated from human faeces, which forms a distinct branch in the order Clostridiales, and proposal of Christensenellaceae fam. nov.	Morotomi M, Nagai F, Watanabe Y	Int J Syst Evol Microbiol	10.1099/ijs.0.026989-0	2011	*
Cultivation	Species-targeted sorting and cultivation of commensal bacteria from the gut microbiome using flow cytometry under anaerobic conditions.	Bellais S, Nehlich M, Ania M, Duquenoy A, Mazier W, van den Engh G, Baijer J, Treichel NS, Clavel T, Belotserkovsky I, Thomas V	Microbiome	10.1186/s40168-021-01206-7	2022	*
Pathogenicity	Gilbert's Syndrome and the Gut Microbiota - Insights From the Case-Control BILIHEALTH Study.	Zohrer PA, Hana CA, Seyed Khoei N, Molzer C, Hormann-Wallner M, Tosevska A, Doberer D, Marculescu R, Bulmer AC, Herbold CW, Berry D, Wagner KH	Front Cell Infect Microbiol	10.3389/fcimb.2021.701109	2021	*
Pathogenicity	The Keystone commensal bacterium Christensenella minuta DSM 22607 displays anti-inflammatory properties both in vitro and in vivo.	Kropp C, Le Corf K, Relizani K, Tambosco K, Martinez C, Chain F, Rawadi G, Langella P, Claus SP, Martin R	Sci Rep	10.1038/s41598-021-90885-1	2021	*
Phylogeny	Taxonomic Description and Genome Sequence of Christensenella intestinihominis sp. nov., a Novel Cholesterol-Lowering Bacterium Isolated From Human Gut.	Zou Y, Xue W, Lin X, Hu T, Liu SW, Sun CH, Luo G, Lv M, Dai Y, Kristiansen K, Xiao L	Front Microbiol	10.3389/fmicb.2021.632361	2021	*
Genetics	Genome Sequence of Christensenella minuta DSM 22607T.	Rosa BA, Hallsworth-Pepin K, Martin J, Wollam A, Mitreva M	Genome Announc	10.1128/genomeA.01451-16	2017	*
	Draft Genome Analysis of Christensenella minuta DSM 22607, exhibiting an unusual expansion of transporter homologs of unknown function.	Coil DA, Jospin G, Eisen JA	J Genomics	10.7150/jgen.43162	2020	*
	Christensenella regulated by Huang-Qi-Ling-Hua-San is a key factor by which to improve type 2 diabetes.	Pan T, Zheng S, Zheng W, Shi C, Ning K, Zhang Q, Xie Y, Xiang H, Xie Q	Front Microbiol	10.3389/fmicb.2022.1022403	2022	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#21228

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 22607

#29932

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26303 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#66794

Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#88575

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID403729.1 )

#26303

IJSEM 144 2012 ( DOI 10.1099/ijs.0.026989-0 , PubMed 21357455 )

* These data were automatically processed and therefore are not curated

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