Strain identifier

BacDive ID: 24747

Type strain: Yes

Species: Christensenella minuta

Strain history: Y. Watanabe A8 (=YIT 12065).

NCBI tax ID(s): 626937 (species)

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General

@ref: 21228

BacDive-ID: 24747

DSM-Number: 22607

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, Gram-negative, rod-shaped

description: Christensenella minuta DSM 22607 is an anaerobe, mesophilic, Gram-negative bacterium that was isolated from faeces from a healthy Japanese male.

NCBI tax id

  • NCBI tax id: 626937
  • Matching level: species

strain history

@refhistory
21228<- Y. Watanabe, Yakult Central Inst. Microbiol. Res., Tokyo, Japan; YIT 12065 <- F. Nagai and M. Morotomi {2008}
67770Y. Watanabe A8 (=YIT 12065).

doi: 10.13145/bacdive24747.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Christensenellaceae
  • genus: Christensenella
  • species: Christensenella minuta
  • full scientific name: Christensenella minuta Morotomi et al. 2012

@ref: 21228

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Christensenellaceae

genus: Christensenella

species: Christensenella minuta

full scientific name: Christensenella minuta Morotomi et al. 2012

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
29932negative0.8-1.9 µm0.4 µmrod-shapedno
69480positive100

pigmentation

  • @ref: 29932
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
21228WILKINS-CHALGREN ANAEROBE BROTH (N2/CO2) (DSMZ Medium 339a)yeshttps://mediadive.dsmz.de/medium/339aName: WILKINS-CHALGREN ANAEROBE BROTH (N2/CO2) (DSMZ Medium 339a) Composition: dehydrated Wilkins-Chalgren medium 33.0 g/l D-Glucose 5.0 g/l L-Cysteine HCl 0.3 g/l Sodium resazurin 0.0005 g/l Distilled water
21228RCM MEDIUM (N2/CO2) (DSMZ Medium 634c)yeshttps://mediadive.dsmz.de/medium/634cName: RCM MEDIUM (N2/CO2) (DSMZ Medium 634c) Composition: dehydrated RCM medium 38.0 g/l Sodium carbonate 1.0 g/l Sodium resazurin 0.0005 g/l Distilled water
21228CHOPPED MEDIUM (N2/CO2) (DSMZ Medium 78b)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium78b.pdf

culture temp

@refgrowthtypetemperaturerange
21228positivegrowth37mesophilic
29932positivegrowth25-43
29932positiveoptimum37mesophilic
67770positivegrowth37mesophilic

culture pH

@refabilitytypepHPH range
29932positivegrowth6.0-9.0alkaliphile
29932positiveoptimum7.5

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
21228anaerobe
29932anaerobe
69480anaerobe99.308

spore formation

@refspore formationconfidence
29932no
69481no94
69480no99.971

halophily

  • @ref: 29932
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: <5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2993222599arabinose+carbon source
2993217234glucose+carbon source
2993237684mannose+carbon source
2993226546rhamnose+carbon source
2993217814salicin+carbon source
2993218222xylose+carbon source

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
21228faeces from a healthy Japanese maleTokyoJapanJPNAsia
67770Human feces

isolation source categories

Cat1Cat2Cat3
#Host#Human#Male
#Host Body Product#Gastrointestinal tract#Feces (Stool)

taxonmaps

  • @ref: 69479
  • File name: preview.99_2088.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_294;96_1147;97_1347;98_1631;99_2088&stattab=map
  • Last taxonomy: Christensenella
  • 16S sequence: AB490809
  • Sequence Identity:
  • Total samples: 17891
  • soil counts: 159
  • aquatic counts: 276
  • animal counts: 17409
  • plant counts: 47

Safety information

risk assessment

  • @ref: 21228
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 21228
  • description: Christensenella minuta gene for 16S rRNA, partial sequence
  • accession: AB490809
  • length: 1497
  • database: ena
  • NCBI tax ID: 626937

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Christensenella minuta DSM 22607GCA_003628755completencbi626937
66792Christensenella minuta strain DSM 22607626937.29wgspatric626937
66792Christensenella minuta strain DSM 22607626937.8wgspatric626937
66792Christensenella minuta strain DSM 22607626937.26completepatric626937
66792Christensenella minuta strain DSM 22607626937.30wgspatric626937
66792Christensenella minuta DSM 226072728369339draftimg626937
66792Christensenella minuta DSM 226072836893620completeimg626937
66792Christensenella minuta DSM 226072832262383draftimg626937
67770Christensenella minuta DSM 22607GCA_001571425scaffoldncbi626937
67770Christensenella minuta DSM 22607GCA_001652705contigncbi626937
67770Christensenella minuta DSM 22607GCA_001678855scaffoldncbi626937

GC content

@refGC-contentmethod
2122851.3high performance liquid chromatography (HPLC)
2993251.3

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno94no
motileno88.779no
flagellatedno97.876yes
gram-positiveyes81.757no
anaerobicyes98.613yes
aerobicno97.735no
halophileno93.425no
spore-formingno75.806no
glucose-utilyes91.6yes
thermophileno90.771yes
glucose-fermentyes54.255no

External links

@ref: 21228

culture collection no.: DSM 22607, JCM 16072, YIT 12065, KCTC 15498

straininfo link

  • @ref: 88575
  • straininfo: 403729

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Metabolism21357455Description of Christensenella minuta gen. nov., sp. nov., isolated from human faeces, which forms a distinct branch in the order Clostridiales, and proposal of Christensenellaceae fam. nov.Morotomi M, Nagai F, Watanabe YInt J Syst Evol Microbiol10.1099/ijs.0.026989-02011Acetic Acid/metabolism, Anaerobiosis, Bacterial Typing Techniques, Base Composition, Butyrates/metabolism, Carbohydrates/analysis, Catalase/metabolism, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/metabolism, Feces/*microbiology, Fermentation, Glucose/metabolism, Gram-Positive Bacteria/*classification/genetics/*isolation & purification/physiology, Humans, Molecular Sequence Data, Oxidoreductases/metabolism, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sucrose/metabolism, Urease/metabolismPhylogeny
Genetics28082495Genome Sequence of Christensenella minuta DSM 22607T.Rosa BA, Hallsworth-Pepin K, Martin J, Wollam A, Mitreva MGenome Announc10.1128/genomeA.01451-162017
32190126Draft Genome Analysis of Christensenella minuta DSM 22607, exhibiting an unusual expansion of transporter homologs of unknown function.Coil DA, Jospin G, Eisen JAJ Genomics10.7150/jgen.431622020
Phylogeny33692769Taxonomic Description and Genome Sequence of Christensenella intestinihominis sp. nov., a Novel Cholesterol-Lowering Bacterium Isolated From Human Gut.Zou Y, Xue W, Lin X, Hu T, Liu SW, Sun CH, Luo G, Lv M, Dai Y, Kristiansen K, Xiao LFront Microbiol10.3389/fmicb.2021.6323612021Genetics
Pathogenicity34075098The Keystone commensal bacterium Christensenella minuta DSM 22607 displays anti-inflammatory properties both in vitro and in vivo.Kropp C, Le Corf K, Relizani K, Tambosco K, Martinez C, Chain F, Rawadi G, Langella P, Claus SP, Martin RSci Rep10.1038/s41598-021-90885-12021Animals, Anti-Inflammatory Agents/*pharmacology, Caco-2 Cells, *Clostridiales, *Colitis/chemically induced/metabolism/therapy, Disease Models, Animal, HT29 Cells, Humans, *Intestinal Mucosa/metabolism/microbiology, Male, MiceMetabolism
Pathogenicity34604105Gilbert's Syndrome and the Gut Microbiota - Insights From the Case-Control BILIHEALTH Study.Zohrer PA, Hana CA, Seyed Khoei N, Molzer C, Hormann-Wallner M, Tosevska A, Doberer D, Marculescu R, Bulmer AC, Herbold CW, Berry D, Wagner KHFront Cell Infect Microbiol10.3389/fcimb.2021.7011092021Case-Control Studies, Clostridiales, Female, *Gastrointestinal Microbiome, *Gilbert Disease/genetics, Humans, RNA, Ribosomal, 16S/geneticsPhylogeny
Cultivation35115054Species-targeted sorting and cultivation of commensal bacteria from the gut microbiome using flow cytometry under anaerobic conditions.Bellais S, Nehlich M, Ania M, Duquenoy A, Mazier W, van den Engh G, Baijer J, Treichel NS, Clavel T, Belotserkovsky I, Thomas VMicrobiome10.1186/s40168-021-01206-72022Anaerobiosis, Animals, Bacteria/metabolism, Faecalibacterium prausnitzii, Flow Cytometry, *Gastrointestinal Microbiome, RabbitsMetabolism
36312936Christensenella regulated by Huang-Qi-Ling-Hua-San is a key factor by which to improve type 2 diabetes.Pan T, Zheng S, Zheng W, Shi C, Ning K, Zhang Q, Xie Y, Xiang H, Xie QFront Microbiol10.3389/fmicb.2022.10224032022

Reference

@idauthorstitledoi/urlcatalogueID_cross_referencepubmedjournal
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
21228Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-22607Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 22607)
29932Barberan A, Caceres Velazquez H, Jones S, Fierer N.Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information10.1128/mSphere.00237-172630328776041
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar SchomburgBRENDA, the ELIXIR core data resource in 2021: new developments and updates10.1093/nar/gkaa102533211880Nucleic Acids Res. 49: D498-D508 2020
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
88575Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID403729.1