Name: Ensifer meliloti BL225C
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 24468

Type strain:

Species: Ensifer meliloti

Strain Designation: BL225C

Culture col. no.: DSM 23914

Strain history: <- A. Mengoni, Univ. Firenze, Italy; BL225C <- C. Scotti, CRA-FLC, Centro Ricerca Istituto Sperimentale Lattiero Caseario, Lodi, Italy

NCBI tax ID(s): 382 (species)

SI-ID 397519

DSM 23914

Special Collections

DSMZ

Other strains from Ensifer meliloti

Section

Ensifer meliloti BL225C is a Gram-negative bacterium that was isolated from root nodules of Medicago sativa.

Gram-negative
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Hyphomicrobiales*
	Family	*Rhizobiaceae*
	Genus	*Ensifer*
	Species	**Ensifer meliloti**
	Full Scientific Name (LPSN)	Ensifer meliloti (Dangeard 1926) Young 2003

	Synonym	Rhizobium meliloti
	Synonym	Sinorhizobium meliloti

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	negative Confidence in %99

[Ref.: #20536]

Incubation period

2-3 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #20536]

Culture medium

RHIZOBIUM MEDIUM (DSMZ Medium 98)

[Ref.: #20536]

Culture medium growth

[Ref.: #20536]

Culture medium link

Medium recipe at MediaDive

[Ref.: #20536]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #20536]			growth	28 °C

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #20536]	Sample type/isolated from	root nodules of Medicago sativa
[Ref.: #20536]	Host species	Medicago sativa
[Ref.: #20536]	Geographic location (country and/or sea, region)	Lodi
[Ref.: #20536]	Country	Italy
[Ref.: #20536]	Country ISO 3 Code	ITA
[Ref.: #20536]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Root nodule

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #20536]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Sinorhizobium meliloti DSM 23914	GCA_900107055	scaffold	GenBank ENA	382 tax ID	*
[Ref.: #66792]	Sinorhizobium meliloti strain DSM 23914	382.1069	wgs	PATRIC	382 tax ID	*
[Ref.: #66792]	Sinorhizobium meliloti DSM 23914	2597490346	draft	JGI IMG/M	382 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Sinorhizobium meliloti DSM 23914 NCBI: GCA_900107055.1
[Ref.: #69480]	gram-positive	gram-positiveⓘ	no	99	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	96.796	no
[Ref.: #69480]	aerobic	aerobicⓘ	yes	87.28	no
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	88.406	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	99	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	yes	67.475	no

Availability in culture collections External links

[Ref.: #20536]

Culture collection no.

DSM 23914

[Ref.: #88337]

SI-ID 397519

Literature:

	Title	Authors	Journal	DOI	Year
Genetics	Exploring the symbiotic pangenome of the nitrogen-fixing bacterium Sinorhizobium meliloti.	Galardini M, Mengoni A, Brilli M, Pini F, Fioravanti A, Lucas S, Lapidus A, Cheng JF, Goodwin L, Pitluck S, Land M, Hauser L, Woyke T, Mikhailova N, Ivanova N, Daligault H, Bruce D, Detter C, Tapia R, Han C, Teshima H, Mocali S, Bazzicalupo M, Biondi EG	BMC Genomics	10.1186/1471-2164-12-235	2011	*
	Competitiveness for Nodule Colonization in Sinorhizobium meliloti: Combined In Vitro-Tagged Strain Competition and Genome-Wide Association Analysis.	Bellabarba A, Bacci G, Decorosi F, Aun E, Azzarello E, Remm M, Giovannetti L, Viti C, Mengoni A, Pini F	mSystems	10.1128/mSystems.00550-21	2021	*
	Mixed Nodule Infection in Sinorhizobium meliloti-Medicago sativa Symbiosis Suggest the Presence of Cheating Behavior.	Checcucci A, Azzarello E, Bazzicalupo M, Galardini M, Lagomarsino A, Mancuso S, Marti L, Marzano MC, Mocali S, Squartini A, Zanardo M, Mengoni A	Front Plant Sci	10.3389/fpls.2016.00835	2016	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20536

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 23914

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#88337

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID397519.1 )

* These data were automatically processed and therefore are not curated

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Sinorhizobium meliloti DSM 23914

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