Strain identifier
BacDive ID: 24468
Type strain: 
Species: Ensifer meliloti
Strain Designation: BL225C
Strain history: <- A. Mengoni, Univ. Firenze, Italy; BL225C <- C. Scotti, CRA-FLC, Centro Ricerca Istituto Sperimentale Lattiero Caseario, Lodi, Italy
NCBI tax ID(s): 382 (species)
version 9.1 (current version)
General
@ref: 20536
BacDive-ID: 24468
DSM-Number: 23914
keywords: genome sequence, Bacteria, Gram-negative
description: Ensifer meliloti BL225C is a Gram-negative bacterium that was isolated from root nodules of Medicago sativa.
NCBI tax id
- NCBI tax id: 382
- Matching level: species
strain history
- @ref: 20536
- history: <- A. Mengoni, Univ. Firenze, Italy; BL225C <- C. Scotti, CRA-FLC, Centro Ricerca Istituto Sperimentale Lattiero Caseario, Lodi, Italy
doi: 10.13145/bacdive24468.20240916.9.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Alphaproteobacteria
- order: Hyphomicrobiales
- family: Rhizobiaceae
- genus: Ensifer
- species: Ensifer meliloti
- full scientific name: Ensifer meliloti (Dangeard 1926) Young 2003
synonyms
@ref synonym 20215 Rhizobium meliloti 20215 Sinorhizobium meliloti
@ref: 20536
domain: Bacteria
phylum: Proteobacteria
class: Alphaproteobacteria
order: Rhizobiales
family: Rhizobiaceae
genus: Ensifer
species: Ensifer meliloti
full scientific name: Ensifer meliloti (Dangeard 1926) Young 2003
strain designation: BL225C
type strain: no
Morphology
cell morphology
- @ref: 69480
- gram stain: negative
- confidence: 99
colony morphology
- @ref: 20536
- incubation period: 2-3 days
Culture and growth conditions
culture medium
- @ref: 20536
- name: RHIZOBIUM MEDIUM (DSMZ Medium 98)
- growth: yes
- link: https://mediadive.dsmz.de/medium/98
- composition: Name: RHIZOBIUM MEDIUM (DSMZ Medium 98) Composition: air-dried garden soil 80.0 g/l Agar 15.0 g/l Mannitol 10.0 g/l Yeast extract 1.0 g/l Na2CO3 0.2 g/l Distilled water
culture temp
- @ref: 20536
- growth: positive
- type: growth
- temperature: 28
Isolation, sampling and environmental information
isolation
- @ref: 20536
- sample type: root nodules of Medicago sativa
- host species: Medicago sativa
- geographic location: Lodi
- country: Italy
- origin.country: ITA
- continent: Europe
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Host | #Plants | #Herbaceous plants (Grass,Crops) |
| #Host Body-Site | #Plant | #Root nodule |
Safety information
risk assessment
- @ref: 20536
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Sinorhizobium meliloti DSM 23914 | GCA_900107055 | scaffold | ncbi | 382 |
| 66792 | Sinorhizobium meliloti strain DSM 23914 | 382.1069 | wgs | patric | 382 |
| 66792 | Sinorhizobium meliloti DSM 23914 | 2597490346 | draft | img | 382 |
Genome-based predictions
predictions
| @ref | trait | model | description | prediction | confidence | training_data |
|---|---|---|---|---|---|---|
| 69480 | gram-positive | gram-positive | Positive reaction to Gram-staining | no | 99 | no |
| 69480 | anaerobic | anaerobic | Ability to grow under anoxygenic conditions (including facultative anaerobes) | no | 96.796 | no |
| 69480 | aerobic | aerobic | Ability to grow under oxygenic conditions (including facultative aerobes) | yes | 87.28 | no |
| 69480 | spore-forming | spore-forming | Ability to form endo- or exospores | no | 88.406 | no |
| 69480 | thermophilic | thermophile | Ability to grow at temperatures above or equal to 45°C | no | 99 | yes |
| 69480 | flagellated | motile2+ | Ability to perform flagellated movement | yes | 67.475 | no |
External links
@ref: 20536
culture collection no.: DSM 23914
straininfo link
- @ref: 88337
- straininfo: 397519
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Genetics | 21569405 | Exploring the symbiotic pangenome of the nitrogen-fixing bacterium Sinorhizobium meliloti. | Galardini M, Mengoni A, Brilli M, Pini F, Fioravanti A, Lucas S, Lapidus A, Cheng JF, Goodwin L, Pitluck S, Land M, Hauser L, Woyke T, Mikhailova N, Ivanova N, Daligault H, Bruce D, Detter C, Tapia R, Han C, Teshima H, Mocali S, Bazzicalupo M, Biondi EG | BMC Genomics | 10.1186/1471-2164-12-235 | 2011 | Genes, Bacterial/genetics, Genome, Bacterial/*genetics, Genomics, Molecular Sequence Annotation, Nitrogen Fixation/*genetics, Phenotype, Regulon/genetics, Sinorhizobium meliloti/*genetics/*metabolism, Species Specificity, Symbiosis/*genetics, Transcription Factors/metabolism | Phenotype |
| 27379128 | Mixed Nodule Infection in Sinorhizobium meliloti-Medicago sativa Symbiosis Suggest the Presence of Cheating Behavior. | Checcucci A, Azzarello E, Bazzicalupo M, Galardini M, Lagomarsino A, Mancuso S, Marti L, Marzano MC, Mocali S, Squartini A, Zanardo M, Mengoni A | Front Plant Sci | 10.3389/fpls.2016.00835 | 2016 | |||
| 34313466 | Competitiveness for Nodule Colonization in Sinorhizobium meliloti: Combined In Vitro-Tagged Strain Competition and Genome-Wide Association Analysis. | Bellabarba A, Bacci G, Decorosi F, Aun E, Azzarello E, Remm M, Giovannetti L, Viti C, Mengoni A, Pini F | mSystems | 10.1128/mSystems.00550-21 | 2021 |
Reference
| @id | authors | title | doi/url | catalogue |
|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |
| 20536 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-23914 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23914) | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
| 88337 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID397519.1 |