Ensifer meliloti 3D0a2 | Type strain | DSM 30135, ATCC 9930, CCUG 27879, LMG 6133

Strain identifier

BacDive ID: 13546

Type strain:

Species: Ensifer meliloti

Strain Designation: 3D0a2

Culture col. no.: DSM 30135, ATCC 9930, CCUG 27879, LMG 6133

Section

Name and taxonomic classification
Morphology and physiology
Culture and growth conditions
Isolation, sampling and environmental information
Application and interaction
Molecular biology
Strain availability

External links

Information on the name and the taxonomic classification.Name and taxonomic classification

[Ref.: #9156]	Domain	Bacteria
[Ref.: #9156]	Phylum	Proteobacteria
[Ref.: #9156]	Class	Alphaproteobacteria
[Ref.: #9156]	Order	Rhizobiales
[Ref.: #9156]	Family	Rhizobiaceae
[Ref.: #9156]	Genus	Ensifer
[Ref.: #9156]	Species	Ensifer meliloti
[Ref.: #9156]	Full Scientific Name	Ensifer meliloti (Dangeard 1926) Young 2003
[Ref.: #9156]	Strain Designation	3D0a2
[Ref.: #9156]	Type strain

		Prokaryotic Nomenclature Up-to-date (PNU)	Taxonomical status	Literature reference
	Last PNU update	14-08-2019 (DD-MM-YYYY)
[Ref.: #20215]	Domain	Bacteria
[Ref.: #20215]	Phylum	Proteobacteria
[Ref.: #20215]	Class	Alphaproteobacteria		Int. J. Syst. Evol. Microbiol. 56:1
[Ref.: #20215]	Family	Rhizobiaceae		Int. J. Syst. Bacteriol. 30:225
[Ref.: #20215]	Genus	Ensifer	gen. nov. (VP)	Int. J. Syst. Bacteriol. 32:339*
[Ref.: #20215]	Species	Ensifer meliloti	comb. nov. (VP)	Int. J. Syst. Evol. Microbiol. 53:2107*
[Ref.: #20215]	Full Scientific Name	Ensifer meliloti (Dangeard 1926) Young 2003

[Ref.: #20215]	Synonym	Rhizobium meliloti
[Ref.: #20215]	Synonym	Sinorhizobium meliloti

Information on morphological and physiological propertiesMorphology and physiology

[Ref.: #9156]

Incubation period

1-2 days

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #9156]		catalase	+	1.11.1.6
[Ref.: #9156]		cytochrome-c oxidase	+	1.9.3.1

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #9156]	6594	+	-	-	-	+	+	-	+	+	+	+	+/-	+	+	-	-	-	+	-	-	+
[Ref.: #9156]	6593	+/-	-	-	-	+	+/-	-	+	+	+	+	+/-	+	+	-	-	-	+	-	-	not determinedn.d.

[Ref.: #48992]

Oxygen tolerance

aerobe

Information on culture and growth conditionsCulture and growth conditions

[Ref.: #9156]

Culture medium

RHIZOBIUM MEDIUM (DSMZ Medium 98), 26°C

[Ref.: #9156]

Culture medium growth

[Ref.: #9156]

Culture medium link

medium recipe provided by DSMZ

[Ref.: #33771]

Culture medium

MEDIUM 296 - for Rhizobium

[Ref.: #33771]

Culture medium growth

[Ref.: #33771]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #9156]	growth	26 ̊C
[Ref.: #33771]	growth	30 ̊C
[Ref.: #48992]	growth	30 ̊C

[Ref.: #9156]	Temperature range	mesophilic
[Ref.: #33771]	Temperature range	mesophilic
[Ref.: #48992]	Temperature range	mesophilic

Information on isolation source, the sampling and environmental conditionsIsolation, sampling and environmental information

[Ref.: #48992]	Sample type/isolated from	Medicago sativa
[Ref.: #48992]	Country	New Zealand
[Ref.: #48992]	Continent	Australia and Oceania

[Ref.: #33771]	Geographic location (country and/or sea, region)	Virginia
[Ref.: #33771]	Country	USA
[Ref.: #33771]	Continent	North America
* marker position based on {}

Information on possible application of the strain and its possible interaction with e.g. potential hostsApplication and interaction

[Ref.: #9156]

Biosafety level

Risk group (German classification)

Information on genomic background e.g. entries in nucleic sequence databassMolecular biology

Sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence database	Sequence accession description	Sequence accession number	Sequence type	Sequence length (bp)	Associated NCBI tax ID
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Sinorhizobium meliloti strain ATCC 9930 23S ribosomal RNA gene, partial sequence	AY244359		2862	382	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Sinorhizobium meliloti strain ATCC 9930 NodC (nodC) gene, partial cds	EF209423		869	382	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Sinorhizobium meliloti nodulation protein A (nodA) gene, complete cds	FJ800094		591	382	*
[Ref.: #20218]	INSDC Sequence	Rhizobium meliloti nitrogenase reductase (nifH) gene, partial transcribed region	M55229		492	382	*
[Ref.: #20218]	INSDC Sequence	Rhizobium meliloti 16S ribosomal RNA, partial	M55241	16S	260	382	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Rhizobium meliloti 23S ribosomal RNA gene, partial sequence	U47355		674	382	*
[Ref.: #9156]	GenBank Direct submission	Sinorhizobium meliloti strain LMTR32 16S ribosomal RNA gene, partial sequence	AY196963	16S	1479	382

Availability in culture collectionsStrain availability

[Ref.: #9156]

Culture collection no.

DSM 30135, ATCC 9930, CCUG 27879, LMG 6133

[Ref.: #9156]

Strain history

<- ATCC <- N.R. Smith, 3D0a2

[Ref.: #20218]

Associated Passport(s) in StrainInfo

53638, 53637

References

#9156

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 30135

#20215

Leibniz Institute DSMZ D.Gleim, M.Kracht, N.Weiss et. al.: Prokaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since .

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#33771 ; Curators of the CIP;
#48992 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 27879
* These References are textmined

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