Ensifer meliloti (DSM 6047, ATCC 4399, Alfalfa 100)

Name: Ensifer meliloti (DSM 6047, ATCC 4399, Alfalfa 100)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 13544

Type strain

Strain Designation Alfalfa 100

Culture col. no. DSM 6047 ATCC 4399 Alfalfa 100

NCBI tax ID(s) 382

Special Collections DSMZ

History DSMZ <- ATCC <- E.B. Fred, Alfalfa 100

Links

Ensifer meliloti Alfalfa 100 is a bacterium of the family Rhizobiaceae.

Bacteria

Name and taxonomic classification

SI-ID 34710

ATCC 4399,DSM 6047

@ref 20215

Last LPSN update 2026-05-29 08:28:04

Domain Bacteria

Phylum Pseudomonadota

Class Alphaproteobacteria

Order Hyphomicrobiales

Family Rhizobiaceae

Genus Ensifer

Species Ensifer meliloti

Full scientific name Ensifer meliloti (Dangeard 1926) Young 2003

Synonyms (4)

Sinorhizobium kummerowiae
Rhizobium meliloti
Sinorhizobium meliloti
Ensifer kummerowiae

BacDive ID	Other strains from **Ensifer meliloti** (11)	Type strain
13546	E. meliloti 3D0a2, DSM 30135, ATCC 9930, CCUG 27879, ... (type strain)
13541	E. meliloti DSM 1981, TAL 380
13542	E. meliloti MVII/1, pRme-MVII/1a to pRme-MVII/1g, DSM ...
13543	E. meliloti 41AK631, pRme41a to pRme41c, DSM 5171
13545	E. meliloti 101 (USDA 477), 3DOa5, Fred 101, DSM 6048, ...
13547	E. meliloti 3D0d4, DSM 30136, ATCC 10310
24467	E. meliloti AK83, DSM 23913
24468	E. meliloti BL225C, DSM 23914
24469	E. meliloti AK58, DSM 23808
24470	E. meliloti BO21CC, DSM 23809
162896	E. meliloti JCM 20363, IAM 12035

Morphology

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
2219	RHIZOBIUM MEDIUM (DSMZ Medium 98)		Medium recipe at MediaDive	Name: RHIZOBIUM MEDIUM (DSMZ Medium 98) Composition: air-dried garden soil 80.0 g/l Agar 15.0 g/l Mannitol 10.0 g/l Yeast extract 1.0 g/l Na2CO3 0.2 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
2219	positive	growth	26

Physiology and metabolism

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68369	17128 ChEBI	adipate	-	assimilation	from API 20NE
68369	29016 ChEBI	arginine	-	hydrolysis	from API 20NE
68369	17634 ChEBI	D-glucose	+	assimilation	from API 20NE
68369	17634 ChEBI	D-glucose	-	fermentation	from API 20NE
68369	16899 ChEBI	D-mannitol	+	assimilation	from API 20NE
68369	16024 ChEBI	D-mannose	+	assimilation	from API 20NE
68369	27689 ChEBI	decanoate	-	assimilation	from API 20NE
68369	4853 ChEBI	esculin	+	hydrolysis	from API 20NE
68369	5291 ChEBI	gelatin	-	hydrolysis	from API 20NE
68369	24265 ChEBI	gluconate	-	assimilation	from API 20NE
68369	30849 ChEBI	L-arabinose	+	assimilation	from API 20NE
68369	25115 ChEBI	malate	+	assimilation	from API 20NE
68369	17306 ChEBI	maltose	+	assimilation	from API 20NE
68369	59640 ChEBI	N-acetylglucosamine	+	assimilation	from API 20NE
68369	17632 ChEBI	nitrate	-	reduction	from API 20NE
68369	27897 ChEBI	tryptophan	-	energy source	from API 20NE
68369	16199 ChEBI	urea	+	hydrolysis	from API 20NE

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68369	35581 ChEBI	indole		from API 20NE

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
68369	35581 ChEBI	indole	-	from API 20NE

Enzymes

@ref	Value	Activity	Ec
68369	arginine dihydrolase	-	3.5.3.6	from API 20NE
68369	beta-glucosidase	+	3.2.1.21	from API 20NE
68369	cytochrome oxidase	+	1.9.3.1	from API 20NE
68369	gelatinase	-		from API 20NE
68369	urease	+	3.5.1.5	from API 20NE

API 20NE

@ref	Reduction of nitratesNO3	TRP	GLU_ Ferm	ADH (Arg)	URE	ESC	GEL	PNPG	GLU_ Assim	ARA	MNE	MAN	NAG	MAL	GNT	CAP	ADI	MLT	CIT	PAC	OX
2219	-	-	-	-	+	+	-	+	+	+	+	+	+	+	-	-	-	+	-	-	+

Isolation, sampling and environmental information

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
2219	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome-based predictions

Literature

References

#2219	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 6047
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#68369	Automatically annotated from API 20NE .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Rate our new design

Content

About us