Strain identifier
BacDive ID: 13544
Type strain: 
Species: Ensifer meliloti
Strain Designation: Alfalfa 100
Strain history: <- ATCC <- E.B. Fred, Alfalfa 100
NCBI tax ID(s): 382 (species)
version 9 (current version)
General
@ref: 2219
BacDive-ID: 13544
DSM-Number: 6047
keywords: Bacteria
description: Ensifer meliloti Alfalfa 100 is a bacterium of the family Rhizobiaceae.
NCBI tax id
- NCBI tax id: 382
- Matching level: species
strain history
- @ref: 2219
- history: <- ATCC <- E.B. Fred, Alfalfa 100
doi: 10.13145/bacdive13544.20240510.9
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Alphaproteobacteria
- order: Hyphomicrobiales
- family: Rhizobiaceae
- genus: Ensifer
- species: Ensifer meliloti
- full scientific name: Ensifer meliloti (Dangeard 1926) Young 2003
synonyms
@ref synonym 20215 Rhizobium meliloti 20215 Sinorhizobium meliloti
@ref: 2219
domain: Bacteria
phylum: Proteobacteria
class: Alphaproteobacteria
order: Rhizobiales
family: Rhizobiaceae
genus: Ensifer
species: Ensifer meliloti
full scientific name: Ensifer meliloti (Dangeard 1926) Young 2003
strain designation: Alfalfa 100
type strain: no
Culture and growth conditions
culture medium
- @ref: 2219
- name: RHIZOBIUM MEDIUM (DSMZ Medium 98)
- growth: yes
- link: https://mediadive.dsmz.de/medium/98
- composition: Name: RHIZOBIUM MEDIUM (DSMZ Medium 98) Composition: air-dried garden soil 80.0 g/l Agar 15.0 g/l Mannitol 10.0 g/l Yeast extract 1.0 g/l Na2CO3 0.2 g/l Distilled water
culture temp
- @ref: 2219
- growth: positive
- type: growth
- temperature: 26
Physiology and metabolism
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 68369 | 25115 | malate | + | assimilation |
| 68369 | 17128 | adipate | - | assimilation |
| 68369 | 27689 | decanoate | - | assimilation |
| 68369 | 24265 | gluconate | - | assimilation |
| 68369 | 17306 | maltose | + | assimilation |
| 68369 | 59640 | N-acetylglucosamine | + | assimilation |
| 68369 | 16899 | D-mannitol | + | assimilation |
| 68369 | 16024 | D-mannose | + | assimilation |
| 68369 | 30849 | L-arabinose | + | assimilation |
| 68369 | 17634 | D-glucose | + | assimilation |
| 68369 | 5291 | gelatin | - | hydrolysis |
| 68369 | 4853 | esculin | + | hydrolysis |
| 68369 | 16199 | urea | + | hydrolysis |
| 68369 | 29016 | arginine | - | hydrolysis |
| 68369 | 17634 | D-glucose | - | fermentation |
| 68369 | 27897 | tryptophan | - | energy source |
| 68369 | 17632 | nitrate | - | reduction |
metabolite production
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 68369 | cytochrome oxidase | + | 1.9.3.1 |
| 68369 | gelatinase | - | |
| 68369 | beta-glucosidase | + | 3.2.1.21 |
| 68369 | urease | + | 3.5.1.5 |
| 68369 | arginine dihydrolase | - | 3.5.3.6 |
API 20NE
| @ref | NO3 | TRP | GLU_ Ferm | ADH Arg | URE | ESC | GEL | PNPG | GLU_ Assim | ARA | MNE | MAN | NAG | MAL | GNT | CAP | ADI | MLT | CIT | PAC | OX |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 2219 | - | - | - | - | + | + | - | + | + | + | + | + | + | + | - | - | - | + | - | - | + |
Safety information
risk assessment
- @ref: 2219
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
External links
@ref: 2219
culture collection no.: DSM 6047, ATCC 4399
straininfo link
- @ref: 82733
- straininfo: 34710
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 2219 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 6047) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-6047 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 68369 | Automatically annotated from API 20NE | |||
| 82733 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID34710.1 | StrainInfo: A central database for resolving microbial strain identifiers |