Cite this strain

"Gluconacetobacter hansenii" (DSM 46602, ATCC 23769, CCM 1808)

BacDive ID 23507

Type strain

Strain Designation 336, BU 336

Culture col. no. DSM 46602 ATCC 23769 CCM 1808 IMET 10313 NCIB 8246 3 more

Special Collections DSMZ

History <- IMET <- CCM <- BUCSAV, 336 <- NCIB <- M. Aschner

Links

"Gluconacetobacter hansenii" 336 is a bacterium that produces polysaccharides and was isolated from vinegar.

polysaccharide production genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 14554

LMG 1524,ATCC 23769,CCM 1808,IMET 10313,NCIB 8246,DSM 46602,NCIMB 8246,IFO14816,NBRC14816,BCRC 10589,CCRC 10589,CCT 1431,NCIM 2529,CDBB 539,VTT E-92101

@ref 20215

Last LPSN update 2026-02-09 11:23:45

Domain Bacteria

Phylum Pseudomonadota

Class Alphaproteobacteria

Order Rhodospirillales

Family Acetobacteraceae

Genus Gluconacetobacter

Species "Gluconacetobacter hansenii"

Full scientific name Gluconacetobacter hansenii Ge et al. 2011

Morphology

Cell morphology

@ref	Gram stain	Confidence
125438	negative	95.771
125439	negative	95.5

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
20227	GLUCONOBACTER OXYDANS MEDIUM (DSMZ Medium 105)		Medium recipe at MediaDive	Name: GLUCONOBACTER OXYDANS MEDIUM (DSMZ Medium 105) Composition: Glucose 100.0 g/l CaCO3 20.0 g/l Agar 15.0 g/l Yeast extract 10.0 g/l Distilled water
20227	YPM MEDIUM (DSMZ Medium 360)		Medium recipe at MediaDive	Name: YPM MEDIUM (DSMZ Medium 360) Composition: Mannitol 25.0 g/l Agar 12.0 g/l Yeast extract 5.0 g/l Peptone 3.0 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
20227	positive	growth	28

Physiology and metabolism

Spore formation

@ref	Spore formation	Confidence
125439		97.1

Compound production

20227

Compoundcellulose

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Food production	#Fermented
#Engineered	#Food production	#Food
#Condition	#Acidic	-

Isolation

@ref	Sample type	Country	Country ISO 3 Code	Continent
20227	vinegar	Israel	ISR	Asia

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
20227	1	Risk group (German classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM16439v1 assembly for Novacetimonas hansenii ATCC 23769	chromosome	GCA_000164395	714995.3	647000257	714995	73.54
66792	ASM164580v1 assembly for Novacetimonas hansenii AY201	scaffold	GCA_001645805	436.3	2744054709	436	52.67

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
124043	Gluconacetobacter hansenii ATCC 23769 gene for 16S rRNA, partial sequence, strain: NBRC 14816.	AB166734	1453	714995
124043	Gluconacetobacter hansenii ATCC 23769 partial 16S rRNA gene, strain LMG 1524	AJ012543	461	714995
124043	Komagataeibacter hansenii strain ATCC 23769 16S ribosomal RNA gene, partial sequence.	KX216691	1374	436

GC content

20227

GC-content (mol%)55.2

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Gluconacetobacter hansenii ATCC 23769
PATRIC: 714995.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	97.10	no
125439	motility	BacteriaNetⓘ	yes	79.10	no
125439	gram_stain	BacteriaNetⓘ	negative	95.50	no
125439	oxygen_tolerance	BacteriaNetⓘ	facultative anaerobe	85.70	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Komagataeibacter hansenii ATCC 23769 ATCC 23769
NCBI: GCA_000164395.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	95.77	no
125438	anaerobic	anaerobicⓘ	no	89.10	no
125438	aerobic	aerobicⓘ	yes	77.55	no
125438	spore-forming	spore-formingⓘ	no	88.06	no
125438	thermophilic	thermophileⓘ	no	98.26	yes
125438	flagellated	motile2+ⓘ	no	60.00	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Study of the pretreatment and hydrolysis of a mixture of coffee husk, cowpea bean husk and cocoa pod for bacterial cellulose production.	Morais KDS, Guilherme EPX, Menezes BDS, Forte MBS, Leal PL, Aguiar-Oliveira E.	Bioprocess Biosyst Eng	10.1007/s00449-025-03158-w	2025
	Enhancing Antioxidant Benefits of Kombucha Through Optimized Glucuronic Acid by Selected Symbiotic Fermentation Culture.	Chou YC, Lin HW, Wang CY, Hsieh CC, Santoso SP, Lin SP, Cheng KC.	Antioxidants (Basel)	10.3390/antiox13111323	2024
	Evaluating a polymicrobial biofilm model for structural components by co-culturing Komagataeibacter hansenii produced bacterial cellulose with Pseudomonas aeruginosa PAO1.	Mahadevaswamy UR, Mugunthan S, Seviour T, Kjelleberg S, Lim S.	Biofilm	10.1016/j.bioflm.2024.100176	2024
	Effect of lyophilization on the bacterial cellulose produced by different Komagataeibacter strains to adsorb epicatechin	Chen SQ, Cao X, Li Z, Zhu J, Li L.	Carbohydrate polymers.		2020
Biotechnology	Sustainability and Influencing Factors in Bacterial Cellulose Production: A Review of the Impact of Microorganisms, Culture Media and Cultivation Methods.	Catarino RPF, Mascareli VAB, Leite da Costa VL, Pavanello ACL, Spinosa WA.	Food Technol Biotechnol	10.17113/ftb.63.03.25.8746	2025
Metabolism	Effect of lyophilization on the bacterial cellulose produced by different Komagataeibacter strains to adsorb epicatechin.	Chen SQ, Cao X, Li Z, Zhu J, Li L.	Carbohydr Polym	10.1016/j.carbpol.2020.116632	2020
	Complete Genome Sequence of Komagataeibacter hansenii Strain SC-3B.	Pfeffer S, Santos R, Ebels M, Bordbar D, Brown RM.	Genome Announc	10.1128/genomea.00169-17	2017
	Complete Genome Sequence of Komagataeibacter hansenii Strain HUM-1.	Pfeffer S, Santos R, Ebels M, Bordbar D, Brown RM.	Genome Announc	10.1128/genomea.00167-17	2017
	Complete Genome Sequence of Komagataeibacter hansenii LMG 23726^T.	Pfeffer S, Santos R, Ebels M, Bordbar D, Brown RM.	Genome Announc	10.1128/genomea.00168-17	2017
Metabolism	Effect of pH Buffer and Carbon Metabolism on the Yield and Mechanical Properties of Bacterial Cellulose Produced by Komagataeibacter hansenii ATCC 53582.	Li Z, Chen SQ, Cao X, Li L, Zhu J, Yu H.	J Microbiol Biotechnol	10.4014/jmb.2010.10054	2021
	Effect of Bacterial Nanocellulose with Chemisorbed Antiseptics on Alveolar Bone Repair in Rats Undergoing Bisphosphonate Therapy.	Rocha MM, Koth VS, Jeffman MW, Salum FG, de Almeida J, Cesca K, Cherubini K.	Pharmaceutics	10.3390/pharmaceutics17010024	2024
	Biosynthesis of Bacterial Nanocellulose from Low-Cost Cellulosic Feedstocks: Effect of Microbial Producer.	Skiba EA, Shavyrkina NA, Skiba MA, Mironova GF, Budaeva VV.	Int J Mol Sci	10.3390/ijms241814401	2023
	Citrate-buffered Yamanaka medium allows to produce high-yield bacterial nanocellulose in static culture using Komagataeibacter strains isolated from apple cider vinegar.	Nunez D, Oyarzun P, Caceres R, Elgueta E, Gamboa M.	Front Bioeng Biotechnol	10.3389/fbioe.2024.1375984	2024
	Genetic modification for enhancing bacterial cellulose production and its applications.	Singhania RR, Patel AK, Tsai ML, Chen CW, Di Dong C.	Bioengineered	10.1080/21655979.2021.1968989	2021
	Advances in the Production of Sustainable Bacterial Nanocellulose from Banana Leaves.	Dager-Lopez D, Chenche O, Ricaurte-Parraga R, Nunez-Rodriguez P, Bajana JM, Fiallos-Cardenas M.	Polymers (Basel)	10.3390/polym16081157	2024
Metabolism	Molecular aspects of bacterial nanocellulose biosynthesis.	Jacek P, Dourado F, Gama M, Bielecki S.	Microb Biotechnol	10.1111/1751-7915.13386	2019
	A novel antimicrobial-containing nanocellulose scaffold for regenerative endodontics.	Kichler V, Teixeira LS, Prado MM, Colla G, Schuldt DPV, Coelho BS, Porto LM, de Almeida J.	Restor Dent Endod	10.5395/rde.2021.46.e20	2021
Genetics	Complete genome sequence of the cellulose-producing strain Komagataeibacter nataicola RZS01.	Zhang H, Xu X, Chen X, Yuan F, Sun B, Xu Y, Yang J, Sun D.	Sci Rep	10.1038/s41598-017-04589-6	2017
	Research progress of the biosynthetic strains and pathways of bacterial cellulose.	Li G, Wang L, Deng Y, Wei Q.	J Ind Microbiol Biotechnol	10.1093/jimb/kuab071	2022
	The Phytohormone Ethylene Enhances Cellulose Production, Regulates CRP/FNRKx Transcription and Causes Differential Gene Expression within the Bacterial Cellulose Synthesis Operon of Komagataeibacter (Gluconacetobacter) xylinus ATCC 53582.	Augimeri RV, Strap JL.	Front Microbiol	10.3389/fmicb.2015.01459	2015
Metabolism	Bacterial cellulose biosynthesis: diversity of operons, subunits, products, and functions.	Romling U, Galperin MY.	Trends Microbiol	10.1016/j.tim.2015.05.005	2015
Genetics	A Combined Metagenomics and Metatranscriptomics Approach to Unravel Costa Rican Cocoa Box Fermentation Processes Reveals Yet Unreported Microbial Species and Functionalities.	Verce M, Schoonejans J, Hernandez Aguirre C, Molina-Bravo R, De Vuyst L, Weckx S.	Front Microbiol	10.3389/fmicb.2021.641185	2021
Metabolism	GqqA, a novel protein in Komagataeibacter europaeus involved in bacterial quorum quenching and cellulose formation.	Valera MJ, Mas A, Streit WR, Mateo E.	Microb Cell Fact	10.1186/s12934-016-0482-y	2016
Genetics	Engineering control of bacterial cellulose production using a genetic toolkit and a new cellulose-producing strain.	Florea M, Hagemann H, Santosa G, Abbott J, Micklem CN, Spencer-Milnes X, de Arroyo Garcia L, Paschou D, Lazenbatt C, Kong D, Chughtai H, Jensen K, Freemont PS, Kitney R, Reeve B, Ellis T.	Proc Natl Acad Sci U S A	10.1073/pnas.1522985113	2016
	Establishing a Role for Bacterial Cellulose in Environmental Interactions: Lessons Learned from Diverse Biofilm-Producing Proteobacteria.	Augimeri RV, Varley AJ, Strap JL.	Front Microbiol	10.3389/fmicb.2015.01282	2015
	Antibacterial Activity of Biocellulose with Oregano Essential Oil against Cronobacter Strains.	Nagmetova G, Berthold-Pluta A, Garbowska M, Kurmanbayev A, Stasiak-Rozanska L	Polymers (Basel)	10.3390/polym12081647	2020
Biotechnology	Evaluation of Different Methods for Cultivating Gluconacetobacter hansenii for Bacterial Cellulose and Montmorillonite Biocomposite Production: Wound-Dressing Applications.	Hodel KVS, Fonseca LMDS, Santos IMDS, Cerqueira JC, Santos-Junior RED, Nunes SB, Barbosa JDV, Machado BAS	Polymers (Basel)	10.3390/polym12020267	2020
Biotechnology	Bacterial cellulose/hyaluronic acid nanocomposites production through co-culturing Gluconacetobacter hansenii and Lactococcus lactis in a two-vessel circulating system.	Liu K, Catchmark JM	Bioresour Technol	10.1016/j.biortech.2019.121715	2019
Metabolism	Enhanced mechanical properties of bacterial cellulose nanocomposites produced by co-culturing Gluconacetobacter hansenii and Escherichia coli under static conditions.	Liu K, Catchmark JM	Carbohydr Polym	10.1016/j.carbpol.2019.04.071	2019
Enzymology	Influence of chemical and physical conditions in selection of Gluconacetobacter hansenii ATCC 23769 strains with high capacity to produce bacterial cellulose for application as sustained antimicrobial drug-release supports.	Lazarini SC, Yamada C, Barud HS, Trovatti E, Corbi PP, Lustri WR	J Appl Microbiol	10.1111/jam.13916	2018
Genetics	Complete Genome Sequence of a Gluconacetobacter hansenii ATCC 23769 Isolate, AY201, Producer of Bacterial Cellulose and Important Model Organism for the Study of Cellulose Biosynthesis.	Pfeffer S, Mehta K, Brown RM Jr	Genome Announc	10.1128/genomeA.00808-16	2016
Metabolism	Construction and in vitro testing of a cellulose dura mater graft.	Goldschmidt E, Cacicedo M, Kornfeld S, Valinoti M, Ielpi M, Ajler PM, Yampolsky C, Rasmussen J, Castro GR, Argibay P	Neurol Res	10.1080/01616412.2015.1122263	2016
Enzymology	AcsA-AcsB: The core of the cellulose synthase complex from Gluconacetobacter hansenii ATCC23769.	McManus JB, Deng Y, Nagachar N, Kao TH, Tien M	Enzyme Microb Technol	10.1016/j.enzmictec.2015.08.015	2015
Metabolism	Isolation and characterization of two cellulose morphology mutants of Gluconacetobacter hansenii ATCC23769 producing cellulose with lower crystallinity.	Deng Y, Nagachar N, Fang L, Luan X, Catchmark JM, Tien M, Kao TH	PLoS One	10.1371/journal.pone.0119504	2015
Metabolism	Characterization of cellulose and other exopolysaccharides produced from Gluconacetobacter strains.	Fang L, Catchmark JM	Carbohydr Polym	10.1016/j.carbpol.2014.09.028	2014
Metabolism	Identification and characterization of non-cellulose-producing mutants of Gluconacetobacter hansenii generated by Tn5 transposon mutagenesis.	Deng Y, Nagachar N, Xiao C, Tien M, Kao TH	J Bacteriol	10.1128/JB.00767-13	2013
Phylogeny	Processing of cellulose synthase (AcsAB) from Gluconacetobacter hansenii 23769.	Iyer PR, Liu YA, Deng Y, McManus JB, Kao TH, Tien M	Arch Biochem Biophys	10.1016/j.abb.2012.12.002	2012
Genetics	Genome sequence of a cellulose-producing bacterium, Gluconacetobacter hansenii ATCC 23769.	Iyer PR, Geib SM, Catchmark J, Kao TH, Tien M	J Bacteriol	10.1128/JB.00588-10	2010
	The Roles of the Various Cellulose Biosynthesis Operons in Komagataeibacter hansenii ATCC 23769.	Bimmer M, Mientus M, Klingl A, Ehrenreich A, Liebl W	Appl Environ Microbiol	10.1128/aem.02460-21	2022
Metabolism	Adaptive laboratory evolution of nanocellulose-producing bacterium.	Vasconcellos VM, Farinas CS, Ximenes E, Slininger P, Ladisch M	Biotechnol Bioeng	10.1002/bit.26997	2019
Phenotype	Structural changes of bacterial nanocellulose pellicles induced by genetic modification of Komagataeibacter hansenii ATCC 23769.	Jacek P, Ryngajllo M, Bielecki S	Appl Microbiol Biotechnol	10.1007/s00253-019-09846-4	2019
Cultivation	Scaffolds for Chondrogenic Cells Cultivation Prepared from Bacterial Cellulose with Relaxed Fibers Structure Induced Genetically.	Jacek P, Szustak M, Kubiak K, Gendaszewska-Darmach E, Ludwicka K, Bielecki S	Nanomaterials (Basel)	10.3390/nano8121066	2018
Phylogeny	Gluconacetobacter hansenii subsp. nov., a high-yield bacterial cellulose producing strain induced by high hydrostatic pressure.	Ge HJ, Du SK, Lin DH, Zhang JN, Xiang JL, Li ZX	Appl Biochem Biotechnol	10.1007/s12010-011-9372-2	2011

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20227	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 46602
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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"Gluconacetobacter hansenii" (DSM 46602, ATCC 23769, CCM 1808)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Spore formation

Compound production

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000164395

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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