Thermoanaerobacter pentosaceus (DSM 25963, CECT 8142, KCTC 4529)

Name: Thermoanaerobacter pentosaceus (DSM 25963, CECT 8142, KCTC 4529)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 23330

Type strain

Strain Designation DTU01

Culture col. no. DSM 25963 CECT 8142 KCTC 4529 VKM B-2752 DTU01

NCBI tax ID(s) 694059

Special Collections DSMZ KCTC Literature strains

History <- A. Tomás, Tech. Univ. of Denmark DTU, Lyngby; DTU01 <- A. Tomás {2009}

Links

Thermoanaerobacter pentosaceus DTU01 is an anaerobe, spore-forming, thermophilic prokaryote that produces alcohol and was isolated from continuously stirred tank reactor for hydrogen production fed with household waste.

alcohol production spore-forming Gram-negative rod-shaped anaerobe thermophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 390303

DSM 25963,KCTC 4529,VKM B-2752,CECT 8142

@ref 20215

Last LPSN update 2026-02-09 11:23:43

Domain Bacteria

Phylum Bacillota

Class Clostridia

Order Thermoanaerobacterales

Family Thermoanaerobacteraceae

Genus Thermoanaerobacter

Species Thermoanaerobacter pentosaceus

Full scientific name Thermoanaerobacter pentosaceus Tomás et al. 2013

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility
30819	negative	1.25 µm	0.5 µm	rod-shaped

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
19364	THERMOANAEROBACTER (BA) MEDIUM (DSMZ Medium 671)		Medium recipe at MediaDive	Name: THERMOANAEROBACTER (BA) MEDIUM (DSMZ Medium 671; with strain-specific modifications) Composition: D-Xylose 3.95648 g/l Cellulose 1.97824 g/l (optional) Na2CO3 0.98912 g/l NH4Cl 0.98912 g/l Yeast extract 0.74184 g/l K2HPO4 x 3 H2O 0.395648 g/l Na2S x 9 H2O 0.24728 g/l MgCl2 x 6 H2O 0.098912 g/l MgSO4 x 7 H2O 0.0296736 g/l Nitrilotriacetic acid 0.0148368 g/l NaCl 0.0098912 g/l MnSO4 x H2O 0.0049456 g/l ZnSO4 x 7 H2O 0.00178042 g/l CoSO4 x 7 H2O 0.00178042 g/l FeSO4 x 7 H2O 0.00098912 g/l CaCl2 x 2 H2O 0.00098912 g/l Sodium resazurin 0.00049456 g/l NiCl2 x 6 H2O 0.000296736 g/l AlK(SO4)2 x 12 H2O 0.000197824 g/l Pyridoxine hydrochloride 9.8912e-05 g/l CuSO4 x 5 H2O 9.8912e-05 g/l Na2MoO4 x 2 H2O 9.8912e-05 g/l H3BO3 9.8912e-05 g/l (DL)-alpha-Lipoic acid 4.9456e-05 g/l Calcium D-(+)-pantothenate 4.9456e-05 g/l Nicotinic acid 4.9456e-05 g/l Thiamine HCl 4.9456e-05 g/l Riboflavin 4.9456e-05 g/l p-Aminobenzoic acid 4.9456e-05 g/l Folic acid 1.97824e-05 g/l Biotin 1.97824e-05 g/l Na2WO4 x 2 H2O 3.95648e-06 g/l Na2SeO3 x 5 H2O 2.96736e-06 g/l Vitamin B12 9.8912e-07 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
19364	positive	growth	70	thermophilic
30819	positive	growth	50-80	hyperthermophilic
30819	positive	optimum	70	thermophilic

Culture pH

@ref	Ability	Type	PH	PH range
30819	positive	optimum	7
30819	positive	growth	5.36-8.5	alkaliphile

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
19364	anaerobe
30819	anaerobe
125439	anaerobe	99.5

Spore formation

30819

Spore formationyes

Compound production

19364

Compoundethanol

Halophily

@ref	Salt	Growth	Tested relation	Concentration
30819	NaCl	positive	growth	0-4 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
30819	22599 ChEBI	arabinose	+	carbon source
30819	17057 ChEBI	cellobiose	+	carbon source
30819	28757 ChEBI	fructose	+	carbon source
30819	28260 ChEBI	galactose	+	carbon source
30819	17234 ChEBI	glucose	+	carbon source
30819	17716 ChEBI	lactose	+	carbon source
30819	29864 ChEBI	mannitol	+	carbon source
30819	37684 ChEBI	mannose	+	carbon source
30819	28053 ChEBI	melibiose	+	carbon source
30819	17992 ChEBI	sucrose	+	carbon source
30819	18222 ChEBI	xylose	+	carbon source

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Built environment	#Container (Reservoir)
#Engineered	#Industrial	#Industrial production

Isolation

@ref	Sample type	Country	Country ISO 3 Code	Continent
19364	continuously stirred tank reactor for hydrogen production fed with household waste	Denmark	DNK	Europe

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
19364	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
124043	ASM3080398v1 assembly for Thermoanaerobacter pentosaceus DSM 25963	contig	GCA_030803985	694059.4	2926563151	694059	60.03

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
19364	Thermoanaerobacter pentosaceus strain DTU01 16S ribosomal RNA gene, partial sequence	GU176611	1560		694059

GC content

@ref	GC-content (mol%)	Method
19364	34.2	sequence analysis
30819	34.2

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Thermoanaerobacter pentosaceus DSM 25963
IMG: 2926563151

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	70.40	no
125439	motility	BacteriaNetⓘ	yes	72.10	no
125439	gram_stain	BacteriaNetⓘ	variable	77.90	no
125439	oxygen_tolerance	BacteriaNetⓘ	anaerobe	99.50	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Fermentation of Mannitol Extracts From Brown Macro Algae by Thermophilic Clostridia.	Chades T, Scully SM, Ingvadottir EM, Orlygsson J.	Front Microbiol	10.3389/fmicb.2018.01931	2018
Metabolism	Ethanol production from glucose and xylose by immobilized Thermoanaerobacter pentosaceus at 70 °C in an up-flow anaerobic sludge blanket (UASB) reactor.	Sittijunda S, Tomas AF, Reungsang A, O-thong S, Angelidaki I.	Bioresour Technol	10.1016/j.biortech.2013.06.056	2013
Metabolism	Extreme thermophilic ethanol production from rapeseed straw: using the newly isolated Thermoanaerobacter pentosaceus and combining it with Saccharomyces cerevisiae in a two-step process.	Tomas AF, Karagoz P, Karakashev D, Angelidaki I.	Biotechnol Bioeng	10.1002/bit.24813	2013
Enzymology	Expression of a heat-stable NADPH-dependent alcohol dehydrogenase in Caldicellulosiruptor bescii results in furan aldehyde detoxification.	Chung D, Verbeke TJ, Cross KL, Westpheling J, Elkins JG.	Biotechnol Biofuels	10.1186/s13068-015-0287-y	2015
	A new search for thermotolerant yeasts, its characterization and optimization using response surface methodology for ethanol production.	Arora R, Behera S, Sharma NK, Kumar S.	Front Microbiol	10.3389/fmicb.2015.00889	2015
Phylogeny	Thermoanaerobacter pentosaceus sp. nov., an anaerobic, extremely thermophilic, high ethanol-yielding bacterium isolated from household waste.	Tomas AF, Karakashev D, Angelidaki I	Int J Syst Evol Microbiol	10.1099/ijs.0.045211-0	2012

References

#19364	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 25963
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#27150	IJSEM 2396 2013 ( DOI 10.1099/ijs.0.045211-0 , PubMed 23178727 )
#30819	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27150
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Thermoanaerobacter pentosaceus (DSM 25963, CECT 8142, KCTC 4529)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Compound production

Halophily

Metabolite utilization

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_030803985

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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