Strain identifier
BacDive ID: 23330
Type strain: 
Species: Thermoanaerobacter pentosaceus
Strain Designation: DTU01
Strain history: <- A. Tomás, Tech. Univ. of Denmark DTU, Lyngby; DTU01 <- A. Tomás {2009}
NCBI tax ID(s): 694059 (species)
version 8.1 (current version)
General
@ref: 19364
BacDive-ID: 23330
DSM-Number: 25963
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, spore-forming, thermophilic, Gram-negative, rod-shaped, alcohol production
description: Thermoanaerobacter pentosaceus DTU01 is an anaerobe, spore-forming, thermophilic bacterium that produces alcohol and was isolated from continuously stirred tank reactor for hydrogen production fed with household waste.
NCBI tax id
- NCBI tax id: 694059
- Matching level: species
strain history
- @ref: 19364
- history: <- A. Tomás, Tech. Univ. of Denmark DTU, Lyngby; DTU01 <- A. Tomás {2009}
doi: 10.13145/bacdive23330.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Clostridia
- order: Thermoanaerobacterales
- family: Thermoanaerobacteraceae
- genus: Thermoanaerobacter
- species: Thermoanaerobacter pentosaceus
- full scientific name: Thermoanaerobacter pentosaceus Tomás et al. 2013
@ref: 19364
domain: Bacteria
phylum: Firmicutes
class: Clostridia
order: Thermoanaerobacterales
family: Thermoanaerobacteraceae
genus: Thermoanaerobacter
species: Thermoanaerobacter pentosaceus
full scientific name: Thermoanaerobacter pentosaceus Tomás et al. 2013
strain designation: DTU01
type strain: yes
Morphology
cell morphology
- @ref: 30819
- gram stain: negative
- cell length: 1.25 µm
- cell width: 0.5 µm
- cell shape: rod-shaped
- motility: no
Culture and growth conditions
culture medium
- @ref: 19364
- name: THERMOANAEROBACTER (BA) MEDIUM (DSMZ Medium 671)
- growth: yes
- link: https://mediadive.dsmz.de/medium/671
- composition: Name: THERMOANAEROBACTER (BA) MEDIUM (DSMZ Medium 671; with strain-specific modifications) Composition: D-Xylose 3.95648 g/l Cellulose 1.97824 g/l (optional) Na2CO3 0.98912 g/l NH4Cl 0.98912 g/l Yeast extract 0.74184 g/l K2HPO4 x 3 H2O 0.395648 g/l Na2S x 9 H2O 0.24728 g/l MgCl2 x 6 H2O 0.098912 g/l MgSO4 x 7 H2O 0.0296736 g/l Nitrilotriacetic acid 0.0148368 g/l NaCl 0.0098912 g/l MnSO4 x H2O 0.0049456 g/l ZnSO4 x 7 H2O 0.00178042 g/l CoSO4 x 7 H2O 0.00178042 g/l FeSO4 x 7 H2O 0.00098912 g/l CaCl2 x 2 H2O 0.00098912 g/l Sodium resazurin 0.00049456 g/l NiCl2 x 6 H2O 0.000296736 g/l AlK(SO4)2 x 12 H2O 0.000197824 g/l Pyridoxine hydrochloride 9.8912e-05 g/l CuSO4 x 5 H2O 9.8912e-05 g/l Na2MoO4 x 2 H2O 9.8912e-05 g/l H3BO3 9.8912e-05 g/l (DL)-alpha-Lipoic acid 4.9456e-05 g/l Calcium D-(+)-pantothenate 4.9456e-05 g/l Nicotinic acid 4.9456e-05 g/l Thiamine HCl 4.9456e-05 g/l Riboflavin 4.9456e-05 g/l p-Aminobenzoic acid 4.9456e-05 g/l Folic acid 1.97824e-05 g/l Biotin 1.97824e-05 g/l Na2WO4 x 2 H2O 3.95648e-06 g/l Na2SeO3 x 5 H2O 2.96736e-06 g/l Vitamin B12 9.8912e-07 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 19364 | positive | growth | 70 | thermophilic |
| 30819 | positive | growth | 50-80 | hyperthermophilic |
| 30819 | positive | optimum | 70 | thermophilic |
culture pH
| @ref | ability | type | pH | PH range |
|---|---|---|---|---|
| 30819 | positive | growth | 5.36-8.5 | alkaliphile |
| 30819 | positive | optimum | 7 |
Physiology and metabolism
oxygen tolerance
| @ref | oxygen tolerance |
|---|---|
| 19364 | anaerobe |
| 30819 | anaerobe |
spore formation
- @ref: 30819
- spore formation: yes
compound production
- @ref: 19364
- compound: ethanol
halophily
- @ref: 30819
- salt: NaCl
- growth: positive
- tested relation: growth
- concentration: 0-4 %
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 30819 | 22599 | arabinose | + | carbon source |
| 30819 | 17057 | cellobiose | + | carbon source |
| 30819 | 28757 | fructose | + | carbon source |
| 30819 | 28260 | galactose | + | carbon source |
| 30819 | 17234 | glucose | + | carbon source |
| 30819 | 17716 | lactose | + | carbon source |
| 30819 | 29864 | mannitol | + | carbon source |
| 30819 | 37684 | mannose | + | carbon source |
| 30819 | 28053 | melibiose | + | carbon source |
| 30819 | 17992 | sucrose | + | carbon source |
| 30819 | 18222 | xylose | + | carbon source |
Isolation, sampling and environmental information
isolation
- @ref: 19364
- sample type: continuously stirred tank reactor for hydrogen production fed with household waste
- country: Denmark
- origin.country: DNK
- continent: Europe
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Engineered | #Built environment | #Container (Reservoir) |
| #Engineered | #Industrial | #Industrial production |
Safety information
risk assessment
- @ref: 19364
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 19364
- description: Thermoanaerobacter pentosaceus strain DTU01 16S ribosomal RNA gene, partial sequence
- accession: GU176611
- length: 1560
- database: ena
- NCBI tax ID: 694059
Genome sequences
- @ref: 66792
- description: Thermoanaerobacter pentosaceus DSM 25963
- accession: 2926563151
- assembly level: draft
- database: img
- NCBI tax ID: 694059
GC content
| @ref | GC-content | method |
|---|---|---|
| 19364 | 34.2 | sequence analysis |
| 30819 | 34.2 |
External links
@ref: 19364
culture collection no.: DSM 25963, CECT 8142, KCTC 4529, VKM B-2752
straininfo link
- @ref: 87547
- straininfo: 390303
literature
- topic: Phylogeny
- Pubmed-ID: 23178727
- title: Thermoanaerobacter pentosaceus sp. nov., an anaerobic, extremely thermophilic, high ethanol-yielding bacterium isolated from household waste.
- authors: Tomas AF, Karakashev D, Angelidaki I
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.045211-0
- year: 2012
- mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Ethanol/*metabolism, Fermentation, *Garbage, Hot Temperature, Molecular Sequence Data, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Thermoanaerobacter/*classification/genetics/isolation & purification, Xylose/metabolism
- topic2: Metabolism
Reference
| @id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed |
|---|---|---|---|---|---|---|
| 19364 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 25963) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-25963 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 30819 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 27150 | 28776041 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 87547 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID390303.1 | StrainInfo: A central database for resolving microbial strain identifiers |