Name: Brockia lithotrophica Kam1851
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 23331

Type strain:

Species: Brockia lithotrophica

Strain Designation: Kam1851

Culture col. no.: DSM 22653, VKM B-2685

Strain history: <- A. Perevalova, Winogradsky Inst. Microbiol., RAS, Moscow, Russia; Kam1851 <- A. Perevalova {2009}

NCBI tax ID(s): 933949 (species)

SI-ID 401606

DSM 22653

Section

Brockia lithotrophica Kam1851 is an anaerobe, spore-forming, thermophilic bacterium that was isolated from sediment-water mixture from a terrestrial hot spring.

Gram-positive
motile
rod-shaped
spore-forming
anaerobe
thermophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Clostridia*
	Order	*Thermoanaerobacterales*
	Family	*Thermoanaerobacteraceae*
	Genus	*Brockia*
	Species	**Brockia lithotrophica**
	Full Scientific Name (LPSN)	Brockia lithotrophica Perevalova et al. 2013

Information on morphological and physiological properties Morphology

[Ref.: #30656]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %98.533

[Ref.: #30656]	Cell length	2.25 µm

[Ref.: #30656]	Cell width	0.35 µm

[Ref.: #30656]	Cell shape	rod-shaped

[Ref.: #30656]	Motility	yes

[Ref.: #19226]

Incubation period

2-3 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #19226]

Culture medium

BROCKIA LITHOTROPHICA MEDIUM (DSMZ Medium 395a)

[Ref.: #19226]

Culture medium growth

[Ref.: #19226]

Culture medium link

Medium recipe at MediaDive

[Ref.: #19226]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #19226]	growth	65 °C
[Ref.: #30656]	growth	46-78 °C
[Ref.: #30656]	optimum	62.5 °C

[Ref.: #19226]	Temperature range	thermophilic
[Ref.: #30656]	Temperature range	thermophilic
[Ref.: #69480]	Temperature range	thermophilic Confidence in %99.999

	pH	Kind of pH		pH
[Ref.: #30656]			optimum	6.5
[Ref.: #30656]			growth	5.5-8.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #19226]	Oxygen tolerance	anaerobe
[Ref.: #30656]	Oxygen tolerance	anaerobe
[Ref.: #69480]	Oxygen tolerance	anaerobe Confidence in %98.001

[Ref.: #30656]	Ability of spore formation	yes
[Ref.: #69481]	Ability of spore formation	yes Confidence in %99
[Ref.: #69480]	Ability of spore formation	no Confidence in %92.459

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #19226]	Sample type/isolated from	sediment-water mixture from a terrestrial hot spring
[Ref.: #19226]	Geographic location (country and/or sea, region)	Kamchatka, Uzon Caldera, East Thermal Field, Zavarzin Pool (54°49' 81.0'' N, 160°01'46.0'' E)
[Ref.: #19226]	Country	Russia
[Ref.: #19226]	Country ISO 3 Code	RUS
[Ref.: #19226]	Continent	Asia
[Ref.: #19226]	Geographic location	54.8392°/160.0290°
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Sediment
#Environmental	#Aquatic	#Thermal spring
#Condition	#Thermophilic (>45°C)	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence HQ540553 (>99% sequence identity) for Brockia lithotrophica subclade from Microbeatlas

Aquatic Samples	61
Soil Samples	16
Animal Samples	45
Plant Samples
Total Samples	122

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #19226]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #19226]	Brockia lithotrophica strain Kam1851 16S ribosomal RNA gene, partial sequence	HQ540553	1458	ENA	933949 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Brockia lithotrophica DSM 22653	GCA_003633725	scaffold	GenBank	933949 tax ID	*
[Ref.: #66792]	Brockia lithotrophica strain DSM 22653	933949.26	wgs	PATRIC	933949 tax ID	*
[Ref.: #66792]	Brockia lithotrophica DSM 22653	2757320359	draft	JGI IMG/M	933949 tax ID	*

[Ref.: #19226]	GC-content	63 mol%	fluorimetric
[Ref.: #30656]	GC-content	63 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Brockia lithotrophica DSM 22653 NCBI: GCA_003633725.1
[Ref.: #69481]	spore-forming	yes	99	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Brockia lithotrophica DSM 22653 NCBI: GCA_003633725.1
[Ref.: #69480]	flagellated	no	86.818	no
[Ref.: #69480]	gram-positive	yes	83.706	yes
[Ref.: #69480]	anaerobic	yes	93.386	yes
[Ref.: #69480]	aerobic	no	95.505	yes
[Ref.: #69480]	halophile	no	88.769	no
[Ref.: #69480]	spore-forming	no	58.194	no
[Ref.: #69480]	glucose-util	yes	79.289	no
[Ref.: #69480]	thermophile	yes	99.999	no
[Ref.: #69480]	motile	yes	86.347	yes
[Ref.: #69480]	glucose-ferment	no	53.938	no

Availability in culture collections External links

[Ref.: #19226]

Culture collection no.

DSM 22653, VKM B-2685

[Ref.: #87548]

SI-ID 401606

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Brockia lithotrophica gen. nov., sp. nov., an anaerobic thermophilic bacterium from a terrestrial hot spring.	Perevalova AA, Kublanov IV, Baslerov RV, Zhang G, Bonch-Osmolovskaya EA	Int J Syst Evol Microbiol	10.1099/ijs.0.041285-0	2012	*

References

#19226

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 22653

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#30656

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26987 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#87548

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID401606.1 )

#26987

IJSEM 479 2013 ( DOI 10.1099/ijs.0.041285-0 , PubMed 22493174 )

* These data were automatically processed and therefore are not curated

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Brockia lithotrophica DSM 22653

Brockia lithotrophica DSM 22653

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