Name: Promicromonospora endophytica DSM 23716
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 23200

Type strain:

Species: Promicromonospora endophytica

Culture col. no.: DSM 23716, JCM 19560, NRRL B-24816, EUM 273

Strain history: DSM 23716 <-- C. Franco EUM 273 <-- O. Kaewkla.

NCBI tax ID(s): 722703 (species)

SI-ID 408386

DSM 23716

Special Collections

Section

Promicromonospora endophytica DSM 23716 is an aerobe, Gram-positive, coccus-shaped bacterium that was isolated from surface sterilized root of Eucalyptus microcarpa.

coccus-shaped
Gram-positive
aerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micrococcales*
	Family	*Promicromonosporaceae*
	Genus	*Promicromonospora*
	Species	**Promicromonospora endophytica**
	Full Scientific Name (LPSN)	Promicromonospora endophytica Kaewkla and Franco 2012

Information on morphological and physiological properties Morphology

[Ref.: #30312]	Gram stain	positive

[Ref.: #30312]	Cell length	0.6 µm

[Ref.: #30312]	Cell width	0.5 µm

[Ref.: #30312]	Cell shape	coccus-shaped

[Ref.: #30312]	Motility	no

[Ref.: #69309]	Colony color	Beige (1001)
[Ref.: #69309]	Cultivation medium used	ISP 6

[Ref.: #69309]	Colony color	Ivory (1014)
[Ref.: #69309]	Cultivation medium used	ISP 7

[Ref.: #69309]	Colony color	Ivory (1014)
[Ref.: #69309]	Cultivation medium used	suter with tyrosine

[Ref.: #69309]	Colony color	Light ivory (1015)
[Ref.: #69309]	Cultivation medium used	ISP 3

[Ref.: #69309]	Colony color	Light ivory (1015)
[Ref.: #69309]	Cultivation medium used	ISP 4

[Ref.: #69309]	Colony color	Light ivory (1015)
[Ref.: #69309]	Cultivation medium used	ISP 5

[Ref.: #69309]	Colony color	Oyster white (1013)
[Ref.: #69309]	Cultivation medium used	suter without tyrosine

[Ref.: #69309]	Colony color	Sand yellow (1002), Oyster white (1013)
[Ref.: #69309]	Cultivation medium used	ISP 2

[Ref.: #19156]	Incubation period	3-7 days

[Ref.: #30312]	Pigment production	no
[Ref.: #69309]	Pigment production	no
[Ref.: #69309]	Pigment name	Melanin
[Ref.: #69309]	Pigment production	no
[Ref.: #69309]	Pigment name	soluble pigment

[Ref.: #69309]	Multicellular complex forming ability	no
[Ref.: #69309]	Multicellular complex name	Aerial mycelium
[Ref.: #69309]	Medium Name (multicellularity)	ISP 2

[Ref.: #69309]	Multicellular complex forming ability	no
[Ref.: #69309]	Multicellular complex name	Aerial mycelium
[Ref.: #69309]	Medium Name (multicellularity)	ISP 3

[Ref.: #69309]	Multicellular complex forming ability	no
[Ref.: #69309]	Multicellular complex name	Aerial mycelium
[Ref.: #69309]	Medium Name (multicellularity)	ISP 4

[Ref.: #69309]	Multicellular complex forming ability	no
[Ref.: #69309]	Multicellular complex name	Aerial mycelium
[Ref.: #69309]	Medium Name (multicellularity)	ISP 5

[Ref.: #69309]	Multicellular complex forming ability	no
[Ref.: #69309]	Multicellular complex name	Aerial mycelium
[Ref.: #69309]	Medium Name (multicellularity)	ISP 6

[Ref.: #69309]	Multicellular complex forming ability	no
[Ref.: #69309]	Multicellular complex name	Aerial mycelium
[Ref.: #69309]	Medium Name (multicellularity)	ISP 7

[Ref.: #69309]	Multicellular complex forming ability	no
[Ref.: #69309]	Multicellular complex name	Aerial mycelium
[Ref.: #69309]	Medium Name (multicellularity)	suter with tyrosine

[Ref.: #69309]	Multicellular complex forming ability	no
[Ref.: #69309]	Multicellular complex name	Aerial mycelium
[Ref.: #69309]	Medium Name (multicellularity)	suter without tyrosine

Multimedia content

[Ref.: #69309]

Caption:

Plates (65, ISP2, ISP3, ISP4, ISP5, ISP7)

[Ref.: #69309]

Intellectual property rights:

Helmholtz-Zentrum für Infektionsforschung GmbH

[Ref.: #69309]

Caption:

Plates (65, ISP2, ISP3, ISP4, ISP5, ISP7)

[Ref.: #69309]

Intellectual property rights:

Helmholtz-Zentrum für Infektionsforschung GmbH

Information on culture and growth conditions Culture and growth conditions

[Ref.: #19156]

Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

[Ref.: #19156]

Culture medium growth

[Ref.: #19156]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #19156]

Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

[Ref.: #19156]

Culture medium growth

[Ref.: #19156]

Culture medium link

Medium recipe at MediaDive

[Ref.: #19156]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #19156]	growth	28 °C
[Ref.: #30312]	growth	15-27 °C
[Ref.: #67770]	growth	28 °C

	pH	Kind of pH		pH
[Ref.: #30312]			growth	06-10

Information on physiology and metabolism Physiology and metabolism

[Ref.: #30312]

Oxygen tolerance

aerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #69309]		NaCl		growth	0-5	%

[Ref.: #67770]

Observation

quinones: MK-9(H₈), MK-9(H₆), MK-9(H₄)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #69309]	arabinose ChEBI	+/-	growth
[Ref.: #30312]	arabinose ChEBI	+	carbon source
[Ref.: #69309]	cellulose ChEBI	+	growth
[Ref.: #68379]	D-glucose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-mannitol ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-ribose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	esculin ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #69309]	fructose ChEBI	+	growth
[Ref.: #68379]	gelatin ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #69309]	glucose ChEBI	+	growth
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #69309]	inositol ChEBI	+/-	growth
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	maltose ChEBI	-	fermentation	* from API Coryne
[Ref.: #69309]	mannose ChEBI	+/-	growth
[Ref.: #30312]	myo-inositol ChEBI	+	carbon source
[Ref.: #68379]	nitrate ChEBI	-	reduction	* from API Coryne
[Ref.: #69309]	raffinose ChEBI	+/-	growth
[Ref.: #69309]	rhamnose ChEBI	+	growth
[Ref.: #30312]	ribitol ChEBI	+	carbon source
[Ref.: #69309]	sucrose ChEBI	+	growth
[Ref.: #68379]	sucrose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	urea ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #69309]	xylose ChEBI	+/-	growth
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68379]	alkaline phosphatase	+	3.1.3.1	* from API Coryne, API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68379]	alpha-glucosidase	+	3.2.1.20	* from API Coryne, API zym
[Ref.: #68382]	alpha-mannosidase	+	3.2.1.24	* from API zym
[Ref.: #68379]	beta-galactosidase	+	3.2.1.23	* from API Coryne, API zym
[Ref.: #68379]	beta-glucosidase	+	3.2.1.21	* from API Coryne, API zym
[Ref.: #68379]	beta-glucuronidase	-	3.2.1.31	* from API Coryne, API zym
[Ref.: #30312]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	+	3.4.11.3	* from API zym
[Ref.: #68379]	gelatinase	+		* from API Coryne
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym
[Ref.: #68379]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API Coryne
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #68379]	pyrazinamidase	-	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	+	3.4.19.3	* from API Coryne
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68379]	urease	-	3.5.1.5	* from API Coryne
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #69309]	3802	-	-	+	+	-	+	+	-	+	-	+	not determinedn.d.	-	-	-	-	-	-	-	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #69309]	5986	not determinedn.d.	+	+/-	+/-	-	+	+	+	-	-	+	+	+/-	+	-	+	+	+	+	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #19156]	Sample type/isolated from	surface sterilized root of Eucalyptus microcarpa
[Ref.: #19156]	Host species	Eucalyptus microcarpa
[Ref.: #19156]	Geographic location (country and/or sea, region)	Adelaide, Bedford Park
[Ref.: #19156]	Country	Australia
[Ref.: #19156]	Country ISO 3 Code	AUS
[Ref.: #19156]	Continent	Australia and Oceania

[Ref.: #67770]	Sample type/isolated from	Root of Eucalyptus microcarpa
[Ref.: #67770]	Host species	Eucalyptus microcarpa
[Ref.: #67770]	Geographic location (country and/or sea, region)	campus of Flinders Univ., Adelaide, South Australia
[Ref.: #67770]	Country	Australia
[Ref.: #67770]	Country ISO 3 Code	AUS
[Ref.: #67770]	Continent	Australia and Oceania
* marker position based on {}

Isolation sources categories

#Host	#Plants	#Tree
#Host Body-Site	#Plant	#Root (Rhizome)
#Host Body-Site	#Plant	#Sterilized plant part

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #19156]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Promicromonospora endophytica gene for 16S ribosomal RNA, partial sequence, strain: JCM 19560	AB923928	1465	GenBank ENA	722703 tax ID
[Ref.: #19156]	Promicromonospora endophytica strain EUM 273 16S ribosomal RNA gene, partial sequence	GU434253	1370	GenBank ENA	722703 tax ID

[Ref.: #19156]	GC-content	71.7 mol%	high performance liquid chromatography (HPLC)
[Ref.: #30312]	GC-content	71.7 mol%

Availability in culture collections External links

[Ref.: #19156]

Culture collection no.

DSM 23716, JCM 19560, NRRL B-24816, EUM 273

[Ref.: #87429]

SI-ID 408386

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Promicromonospora endophytica sp. nov., an endophytic actinobacterium isolated from the root of an Australian native Grey Box tree.	Kaewkla O, Franco CMM	Int J Syst Evol Microbiol	10.1099/ijs.0.033258-0	2011	*

References

#19156

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 23716

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#30312

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26653 (see below)

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#69309

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#87429

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID408386.1 )

#26653

IJSEM 1687 2012 ( DOI 10.1099/ijs.0.033258-0 , PubMed 21890721 )

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Promicromonospora endophytica in external resources:
SILVA
BRENDA
PANGAEA
GBIF