Name: Promicromonospora endophytica DSM 23716
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 23200

Type strain:

Species: Promicromonospora endophytica

Culture col. no.: DSM 23716, JCM 19560, NRRL B-24816, EUM 273

Strain history: DSM 23716 <-- C. Franco EUM 273 <-- O. Kaewkla.

NCBI tax ID(s): 722703 (species)

SI-ID 408386

DSM 23716

Section

Promicromonospora endophytica DSM 23716 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from surface sterilized root of Eucalyptus microcarpa.

coccus-shaped
Gram-positive
aerobe
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micrococcales*
	Family	*Promicromonosporaceae*
	Genus	*Promicromonospora*
	Species	**Promicromonospora endophytica**
	Full Scientific Name (LPSN)	Promicromonospora endophytica Kaewkla and Franco 2012

Information on morphological and physiological properties Morphology

[Ref.: #30312]	Gram stain	positive

[Ref.: #30312]	Cell length	0.6 µm

[Ref.: #30312]	Cell width	0.5 µm

[Ref.: #30312]	Cell shape	coccus-shaped

[Ref.: #30312]	Motility	no

[Ref.: #69309]	Colony color	Beige (1001)
[Ref.: #69309]	Cultivation medium used	ISP 6

[Ref.: #69309]	Colony color	Ivory (1014)
[Ref.: #69309]	Cultivation medium used	ISP 7

[Ref.: #69309]	Colony color	Ivory (1014)
[Ref.: #69309]	Cultivation medium used	suter with tyrosine

[Ref.: #69309]	Colony color	Light ivory (1015)
[Ref.: #69309]	Cultivation medium used	ISP 3

[Ref.: #69309]	Colony color	Light ivory (1015)
[Ref.: #69309]	Cultivation medium used	ISP 4

[Ref.: #69309]	Colony color	Light ivory (1015)
[Ref.: #69309]	Cultivation medium used	ISP 5

[Ref.: #69309]	Colony color	Oyster white (1013)
[Ref.: #69309]	Cultivation medium used	suter without tyrosine

[Ref.: #69309]	Colony color	Sand yellow (1002), Oyster white (1013)
[Ref.: #69309]	Cultivation medium used	ISP 2

[Ref.: #19156]	Incubation period	3-7 days

[Ref.: #30312]	Pigment production	no
[Ref.: #69309]	Pigment production	no
[Ref.: #69309]	Pigment name	Melanin
[Ref.: #69309]	Pigment production	no
[Ref.: #69309]	Pigment name	soluble pigment

[Ref.: #69309]	Multicellular complex forming ability	no
[Ref.: #69309]	Multicellular complex name	Aerial mycelium
[Ref.: #69309]	Medium Name (multicellularity)	ISP 2

[Ref.: #69309]	Multicellular complex forming ability	no
[Ref.: #69309]	Multicellular complex name	Aerial mycelium
[Ref.: #69309]	Medium Name (multicellularity)	ISP 3

[Ref.: #69309]	Multicellular complex forming ability	no
[Ref.: #69309]	Multicellular complex name	Aerial mycelium
[Ref.: #69309]	Medium Name (multicellularity)	ISP 4

[Ref.: #69309]	Multicellular complex forming ability	no
[Ref.: #69309]	Multicellular complex name	Aerial mycelium
[Ref.: #69309]	Medium Name (multicellularity)	ISP 5

[Ref.: #69309]	Multicellular complex forming ability	no
[Ref.: #69309]	Multicellular complex name	Aerial mycelium
[Ref.: #69309]	Medium Name (multicellularity)	ISP 6

[Ref.: #69309]	Multicellular complex forming ability	no
[Ref.: #69309]	Multicellular complex name	Aerial mycelium
[Ref.: #69309]	Medium Name (multicellularity)	ISP 7

[Ref.: #69309]	Multicellular complex forming ability	no
[Ref.: #69309]	Multicellular complex name	Aerial mycelium
[Ref.: #69309]	Medium Name (multicellularity)	suter with tyrosine

[Ref.: #69309]	Multicellular complex forming ability	no
[Ref.: #69309]	Multicellular complex name	Aerial mycelium
[Ref.: #69309]	Medium Name (multicellularity)	suter without tyrosine

Multimedia content

[Ref.: #69309]

Caption:

Plates (65, ISP2, ISP3, ISP4, ISP5, ISP7)

[Ref.: #69309]

Intellectual property rights:

Helmholtz-Zentrum für Infektionsforschung GmbH

[Ref.: #69309]

Caption:

Plates (65, ISP2, ISP3, ISP4, ISP5, ISP7)

[Ref.: #69309]

Intellectual property rights:

Helmholtz-Zentrum für Infektionsforschung GmbH

Information on culture and growth conditions Culture and growth conditions

[Ref.: #19156]

Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

[Ref.: #19156]

Culture medium growth

[Ref.: #19156]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #19156]

Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

[Ref.: #19156]

Culture medium growth

[Ref.: #19156]

Culture medium link

Medium recipe at MediaDive

[Ref.: #19156]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #19156]	growth	28 °C
[Ref.: #30312]	growth	15-27 °C
[Ref.: #67770]	growth	28 °C

[Ref.: #19156]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #30312]			growth	06-10

Information on physiology and metabolism Physiology and metabolism

[Ref.: #30312]

Oxygen tolerance

aerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #69309]		NaCl		growth	0-5	%

[Ref.: #67770]

Observation

quinones: MK-9(H₈), MK-9(H₆), MK-9(H₄)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #69309]	arabinose ChEBI	+/-	growth
[Ref.: #30312]	arabinose ChEBI	+	carbon source
[Ref.: #69309]	cellulose ChEBI	+	growth
[Ref.: #68379]	D-glucose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-mannitol ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-ribose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	esculin ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #69309]	fructose ChEBI	+	growth
[Ref.: #68379]	gelatin ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #69309]	glucose ChEBI	+	growth
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #69309]	inositol ChEBI	+/-	growth
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	maltose ChEBI	-	fermentation	* from API Coryne
[Ref.: #69309]	mannose ChEBI	+/-	growth
[Ref.: #30312]	myo-inositol ChEBI	+	carbon source
[Ref.: #68379]	nitrate ChEBI	-	reduction	* from API Coryne
[Ref.: #69309]	raffinose ChEBI	+/-	growth
[Ref.: #69309]	rhamnose ChEBI	+	growth
[Ref.: #30312]	ribitol ChEBI	+	carbon source
[Ref.: #69309]	sucrose ChEBI	+	growth
[Ref.: #68379]	sucrose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	urea ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #69309]	xylose ChEBI	+/-	growth
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68379]	alkaline phosphatase	+	3.1.3.1	* from API Coryne, API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68379]	alpha-glucosidase	+	3.2.1.20	* from API Coryne, API zym
[Ref.: #68382]	alpha-mannosidase	+	3.2.1.24	* from API zym
[Ref.: #68379]	beta-galactosidase	+	3.2.1.23	* from API Coryne, API zym
[Ref.: #68379]	beta-glucosidase	+	3.2.1.21	* from API Coryne, API zym
[Ref.: #68379]	beta-glucuronidase	-	3.2.1.31	* from API Coryne, API zym
[Ref.: #30312]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	+	3.4.11.3	* from API zym
[Ref.: #68379]	gelatinase	+		* from API Coryne
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym
[Ref.: #68379]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API Coryne
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #68379]	pyrazinamidase	-	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	+	3.4.19.3	* from API Coryne
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68379]	urease	-	3.5.1.5	* from API Coryne
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #69309]	3802	-	-	+	+	-	+	+	-	+	-	+	not determinedn.d.	-	-	-	-	-	-	-	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #69309]	5986	not determinedn.d.	+	+/-	+/-	-	+	+	+	-	-	+	+	+/-	+	-	+	+	+	+	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #19156]	Sample type/isolated from	surface sterilized root of Eucalyptus microcarpa
[Ref.: #19156]	Host species	Eucalyptus microcarpa
[Ref.: #19156]	Geographic location (country and/or sea, region)	Adelaide, Bedford Park
[Ref.: #19156]	Country	Australia
[Ref.: #19156]	Country ISO 3 Code	AUS
[Ref.: #19156]	Continent	Australia and Oceania

[Ref.: #67770]	Sample type/isolated from	Root of Eucalyptus microcarpa
[Ref.: #67770]	Host species	Eucalyptus microcarpa
[Ref.: #67770]	Geographic location (country and/or sea, region)	campus of Flinders Univ., Adelaide, South Australia
[Ref.: #67770]	Country	Australia
[Ref.: #67770]	Country ISO 3 Code	AUS
[Ref.: #67770]	Continent	Australia and Oceania
* marker position based on {}

Isolation sources categories

#Host	#Plants	#Tree
#Host Body-Site	#Plant	#Root (Rhizome)
#Host Body-Site	#Plant	#Sterilized plant part

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #19156]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Promicromonospora endophytica gene for 16S ribosomal RNA, partial sequence, strain: JCM 19560	AB923928	1465	ENA	722703 tax ID
[Ref.: #19156]	Promicromonospora endophytica strain EUM 273 16S ribosomal RNA gene, partial sequence	GU434253	1370	ENA	722703 tax ID

[Ref.: #19156]	GC-content	71.7 mol%	high performance liquid chromatography (HPLC)
[Ref.: #30312]	GC-content	71.7 mol%

Availability in culture collections External links

[Ref.: #19156]

Culture collection no.

DSM 23716, JCM 19560, NRRL B-24816, EUM 273

[Ref.: #87429]

SI-ID 408386

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Promicromonospora endophytica sp. nov., an endophytic actinobacterium isolated from the root of an Australian native Grey Box tree.	Kaewkla O, Franco CMM	Int J Syst Evol Microbiol	10.1099/ijs.0.033258-0	2011	*

References

#19156

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 23716

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#30312

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26653 (see below)

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#69309

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#87429

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID408386.1 )

#26653

IJSEM 1687 2012 ( DOI 10.1099/ijs.0.033258-0 , PubMed 21890721 )

* These data were automatically processed and therefore are not curated

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