Name: Alloprevotella rava 81/4 12
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 23199

Type strain:

Species: Alloprevotella rava

Strain Designation: 81/4 12

Culture col. no.: DSM 22548, CCUG 58091, KCTC 15416

Strain history: <- CCUG <- J Downes, Kings College, London, UK

NCBI tax ID(s): 671218 (species)

Section

Alloprevotella rava 81/4 12 is an anaerobe, mesophilic, Gram-negative human pathogen that was isolated from human periodontitis patient, oral cavity, subgingival plaque .

Gram-negative
ovoid-shaped
anaerobe
human pathogen
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacteroidota*
	Class	*Bacteroidia*
	Order	*Bacteroidales*
	Family	*Prevotellaceae*
	Genus	*Alloprevotella*
	Species	**Alloprevotella rava**
	Full Scientific Name (PNU)	Alloprevotella rava Downes et al. 2013

Information on morphological and physiological properties Morphology

[Ref.: #30660]	Gram stain	negative
[Ref.: #30660]	Cell length	3 µm
[Ref.: #30660]	Cell width	0.55 µm
[Ref.: #30660]	Cell shape	ovoid-shaped
[Ref.: #30660]	Motility	no

[Ref.: #67771]	Gram stain	negative

[Ref.: #67771]	Motility	no

[Ref.: #19290]	Incubation period	2-3 days

[Ref.: #61591]	Incubation period	2 days

[Ref.: #30660]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #19290]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #19290]

Culture medium growth

[Ref.: #19290]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #19290]

Culture medium composition

expand / minimize

[Ref.: #19290]

Culture medium

FASTIDIOUS ANAEROBE AGAR (DSMZ Medium 1203)

[Ref.: #19290]

Culture medium growth

[Ref.: #19290]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #19290]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #19290]	growth	37 °C
[Ref.: #30660]	growth	30-42 °C
[Ref.: #30660]	optimum	35 °C
[Ref.: #61591]	growth	35 °C
[Ref.: #67771]	growth	37 °C

[Ref.: #19290]	Temperature range	mesophilic
[Ref.: #30660]	Temperature range	mesophilic
[Ref.: #61591]	Temperature range	mesophilic
[Ref.: #67771]	Temperature range	mesophilic

	pH	Kind of pH		pH	pH range
[Ref.: #30660]			optimum	7
[Ref.: #30660]			growth	06-07

Information on physiology and metabolism Physiology and metabolism

[Ref.: #19290]	Oxygen tolerance	anaerobe
[Ref.: #30660]	Oxygen tolerance	anaerobe
[Ref.: #61591]	Oxygen tolerance	anaerobe
[Ref.: #67771]	Oxygen tolerance	anaerobe

[Ref.: #30660]

Observation

aggregates in chains

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68380]	arginine ChEBI	-	hydrolysis	* from API rID32A
[Ref.: #68380]	D-mannose ChEBI	-	fermentation	* from API rID32A
[Ref.: #30660]	fructose ChEBI	+	carbon source
[Ref.: #30660]	glucose ChEBI	+	carbon source
[Ref.: #68380]	L-glutamate ChEBI	-	degradation	* from API rID32A
[Ref.: #30660]	lactose ChEBI	+	carbon source
[Ref.: #30660]	maltose ChEBI	+	carbon source
[Ref.: #30660]	mannose ChEBI	+	carbon source
[Ref.: #68380]	nitrate ChEBI	-	reduction	* from API rID32A
[Ref.: #68380]	raffinose ChEBI	-	fermentation	* from API rID32A
[Ref.: #68380]	tryptophan ChEBI	-	energy source	* from API rID32A
[Ref.: #68380]	urea ChEBI	-	hydrolysis	* from API rID32A
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite
[Ref.: #68380]		indole ChEBI	* from API rID32A

	Physiological tests	Metabolite	Indole test
[Ref.: #68380]		indole ChEBI	-	* from API rID32A

	Enzyme	Enzyme activity	EC number
[Ref.: #68380]	alanine arylamidase	+	3.4.11.2	* from API rID32A
[Ref.: #30660]	alkaline phosphatase	+	3.1.3.1
[Ref.: #68380]	alkaline phosphatase	+	3.1.3.1	* from API rID32A
[Ref.: #68380]	alpha-arabinosidase	-	3.2.1.55	* from API rID32A
[Ref.: #68380]	alpha-fucosidase	-	3.2.1.51	* from API rID32A
[Ref.: #30660]	alpha-galactosidase	+	3.2.1.22
[Ref.: #68380]	alpha-galactosidase	-	3.2.1.22	* from API rID32A
[Ref.: #68380]	alpha-glucosidase	-	3.2.1.20	* from API rID32A
[Ref.: #68380]	arginine dihydrolase	-	3.5.3.6	* from API rID32A
[Ref.: #68380]	beta-galactosidase	+	3.2.1.23	* from API rID32A
[Ref.: #68380]	beta-Galactosidase 6-phosphate	-		* from API rID32A
[Ref.: #68380]	beta-glucosidase	-	3.2.1.21	* from API rID32A
[Ref.: #68380]	beta-glucuronidase	-	3.2.1.31	* from API rID32A
[Ref.: #30660]	gelatinase	+
[Ref.: #68380]	glutamate decarboxylase	-	4.1.1.15	* from API rID32A
[Ref.: #68380]	glutamyl-glutamate arylamidase	-		* from API rID32A
[Ref.: #68380]	glycin arylamidase	-		* from API rID32A
[Ref.: #68380]	histidine arylamidase	-		* from API rID32A
[Ref.: #68380]	L-arginine arylamidase	-		* from API rID32A
[Ref.: #68380]	leucine arylamidase	-	3.4.11.1	* from API rID32A
[Ref.: #68380]	leucyl glycin arylamidase	-	3.4.11.1	* from API rID32A
[Ref.: #68380]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API rID32A
[Ref.: #68380]	phenylalanine arylamidase	-		* from API rID32A
[Ref.: #68380]	proline-arylamidase	-	3.4.11.5	* from API rID32A
[Ref.: #68380]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API rID32A
[Ref.: #68380]	serine arylamidase	-		* from API rID32A
[Ref.: #68380]	tryptophan deaminase	-	4.1.99.1	* from API rID32A
[Ref.: #68380]	tyrosine arylamidase	-		* from API rID32A
[Ref.: #68380]	urease	-	3.5.1.5	* from API rID32A
	Only first 10 entries are displayed. Click here to see all.

API® rID32A:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Urease/urea hydrolysisURE	Arginine dihydrolaseADH (Arg)	alpha-Galactosidasealpha GAL	beta-Galactosidasebeta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha-Glucosidasealpha GLU	beta-Glucosidasebeta GLU	alpha-Arabinosidasealpha ARA	beta-Glucuronidasebeta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	Fermentation of D-mannoseMNE	Fermentation of raffinoseRAF	Glutamate decarboxylaseGDC	alpha-Fucosidasealpha FUC	Reduction of nitrateNIT	Indole productionIND	Alkaline phosphatasePAL	L-arginine arylamidaseArgA	Proline arylamidaseProA	Leucyl glycin arylamidaseLGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	Pyrrolidonyl arylamidasePyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
[Ref.: #19290]	11114	-	-	-	+	-	-	-	-	-	+	-	-	-	-	-	-	+	-	-	-	-	-	-	-	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #19290]	Sample type/isolated from	human periodontitis patient, oral cavity, subgingival plaque (>8 mm periodontal pocket)
[Ref.: #19290]	Geographic location (country and/or sea, region)	Cardiff
[Ref.: #19290]	Country	United Kingdom
[Ref.: #19290]	Country ISO 3 Code	GBR
[Ref.: #19290]	Continent	Europe

[Ref.: #61591]	Sample type/isolated from	Human oral cavity,8mm periodental pocket
[Ref.: #61591]	Sampling date	1985-11-01
[Ref.: #61591]	Geographic location (country and/or sea, region)	Cardiff
[Ref.: #61591]	Country	United Kingdom
[Ref.: #61591]	Country ISO 3 Code	GBR
[Ref.: #61591]	Continent	Europe

[Ref.: #67771]	Sample type/isolated from	From human periodontitis patient, oral cavity, subgingival plaque(>8 mm periodontal pocket)
[Ref.: #67771]	Country	United Kingdom
[Ref.: #67771]	Country ISO 3 Code	GBR
[Ref.: #67771]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Infection	#Inflammation	-
#Host	#Human	-
#Host Body-Site	#Oral cavity and airways	#Periodontal pocket
#Host Body-Site	#Oral cavity and airways	#Subgingival plaque

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence JQ039189 (>99% sequence identity) for Alloprevotella rava subclade from Microbeatlas

Aquatic Samples	96
Soil Samples	103
Animal Samples	9985
Plant Samples	31
Total Samples	10215

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #19290]	Pathogenicity (human)	yes, in single cases
[Ref.: #19290]	Biosafety level	1	Risk group (German classification) According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #19290]	Alloprevotella rava strain 81/4-12 16S ribosomal RNA gene, partial sequence	JQ039189	1455	ENA	671218 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Alloprevotella rava DSM 22548	GCA_014195585	contig	GenBank	671218 tax ID	*
[Ref.: #66792]	Alloprevotella rava strain DSM 22548	671218.7	wgs	PATRIC	671218 tax ID	*
[Ref.: #66792]	Alloprevotella rava DSM 22548	2827976805	draft	JGI IMG/M	671218 tax ID	*

[Ref.: #19290]	GC-content	47 mol%	high performance liquid chromatography (HPLC)
[Ref.: #30660]	GC-content	47 mol%
[Ref.: #67771]	GC-content	47.0 mol%

Availability in culture collections External links

[Ref.: #19290]

Culture collection no.

DSM 22548, CCUG 58091, KCTC 15416

[Ref.: #20218]

Associated Passport(s) in StrainInfo

885559, 885558

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Description of Alloprevotella rava gen. nov., sp. nov., isolated from the human oral cavity, and reclassification of Prevotella tannerae Moore et al. 1994 as Alloprevotella tannerae gen. nov., comb. nov.	Downes J, Dewhirst FE, Tanner ACR, Wade WG	Int J Syst Evol Microbiol	10.1099/ijs.0.041376-0	2012	*

References

#19290

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 22548

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#30660

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26991 (see below)

#61591 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 58091

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#68380

Automatically annotated from API rID32A .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#26991

IJSEM 1214 2013 ( DOI 10.1099/ijs.0.041376-0 , PubMed 22753527 )

* These data were automatically processed and therefore are not curated

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