Strain identifier
BacDive ID: 23200
Type strain:
Species: Promicromonospora endophytica
Strain history: DSM 23716 <-- C. Franco EUM 273 <-- O. Kaewkla.
NCBI tax ID(s): 722703 (species)
General
@ref: 19156
BacDive-ID: 23200
DSM-Number: 23716
keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive, coccus-shaped
description: Promicromonospora endophytica DSM 23716 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from surface sterilized root of Eucalyptus microcarpa.
NCBI tax id
- NCBI tax id: 722703
- Matching level: species
strain history
@ref | history |
---|---|
19156 | <- C. Franco, Flinders University Australia; EUM 273 <- O. Kaewkla |
67770 | DSM 23716 <-- C. Franco EUM 273 <-- O. Kaewkla. |
doi: 10.13145/bacdive23200.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Micrococcales
- family: Promicromonosporaceae
- genus: Promicromonospora
- species: Promicromonospora endophytica
- full scientific name: Promicromonospora endophytica Kaewkla and Franco 2012
@ref: 19156
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Promicromonosporaceae
genus: Promicromonospora
species: Promicromonospora endophytica
full scientific name: Promicromonospora endophytica Kaewkla and Franco 2012
type strain: yes
Morphology
cell morphology
- @ref: 30312
- gram stain: positive
- cell length: 0.6 µm
- cell width: 0.5 µm
- cell shape: coccus-shaped
- motility: no
colony morphology
@ref | colony color | medium used | incubation period |
---|---|---|---|
69309 | Beige (1001) | ISP 6 | |
19156 | 3-7 days | ||
69309 | Ivory (1014) | ISP 7 | |
69309 | Ivory (1014) | suter with tyrosine | |
69309 | Light ivory (1015) | ISP 3 | |
69309 | Light ivory (1015) | ISP 4 | |
69309 | Light ivory (1015) | ISP 5 | |
69309 | Oyster white (1013) | suter without tyrosine | |
69309 | Sand yellow (1002), Oyster white (1013) | ISP 2 |
multicellular morphology
@ref | forms multicellular complex | complex name | medium name |
---|---|---|---|
69309 | no | Aerial mycelium | ISP 2 |
69309 | no | Aerial mycelium | ISP 3 |
69309 | no | Aerial mycelium | ISP 4 |
69309 | no | Aerial mycelium | ISP 5 |
69309 | no | Aerial mycelium | ISP 6 |
69309 | no | Aerial mycelium | ISP 7 |
69309 | no | Aerial mycelium | suter with tyrosine |
69309 | no | Aerial mycelium | suter without tyrosine |
pigmentation
@ref | production | name |
---|---|---|
30312 | no | |
69309 | no | Melanin |
69309 | no | soluble pigment |
multimedia
@ref | multimedia content | caption | intellectual property rights |
---|---|---|---|
69309 | DSM_23716_image3.jpeg | Plates (65, ISP2, ISP3, ISP4, ISP5, ISP7) | Helmholtz-Zentrum für Infektionsforschung GmbH |
69309 | DSM_23716_image4.jpeg | Plates (65, ISP2, ISP3, ISP4, ISP5, ISP7) | Helmholtz-Zentrum für Infektionsforschung GmbH |
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
19156 | TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) | yes | https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium535.pdf | |
19156 | GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) | yes | https://mediadive.dsmz.de/medium/65 | Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
19156 | positive | growth | 28 | mesophilic |
30312 | positive | growth | 15-27 | |
67770 | positive | growth | 28 | mesophilic |
culture pH
- @ref: 30312
- ability: positive
- type: growth
- pH: 06-10
- PH range: alkaliphile
Physiology and metabolism
oxygen tolerance
- @ref: 30312
- oxygen tolerance: aerobe
halophily
- @ref: 69309
- salt: NaCl
- growth: positive
- tested relation: growth
- concentration: 0-5 %
observation
- @ref: 67770
- observation: quinones: MK-9(H8), MK-9(H6), MK-9(H4)
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
69309 | 22599 | arabinose | +/- | growth |
30312 | 15963 | ribitol | + | carbon source |
30312 | 22599 | arabinose | + | carbon source |
30312 | 17268 | myo-inositol | + | carbon source |
69309 | 62968 | cellulose | + | growth |
69309 | 28757 | fructose | + | growth |
69309 | 17234 | glucose | + | growth |
69309 | 17268 | inositol | +/- | growth |
69309 | 37684 | mannose | +/- | growth |
69309 | 16634 | raffinose | +/- | growth |
69309 | 26546 | rhamnose | + | growth |
69309 | 17992 | sucrose | + | growth |
69309 | 18222 | xylose | +/- | growth |
68379 | 17632 | nitrate | - | reduction |
68379 | 4853 | esculin | + | hydrolysis |
68379 | 16199 | urea | - | hydrolysis |
68379 | 5291 | gelatin | + | hydrolysis |
68379 | 17634 | D-glucose | - | fermentation |
68379 | 16988 | D-ribose | - | fermentation |
68379 | 65327 | D-xylose | - | fermentation |
68379 | 16899 | D-mannitol | - | fermentation |
68379 | 17306 | maltose | - | fermentation |
68379 | 17716 | lactose | - | fermentation |
68379 | 17992 | sucrose | - | fermentation |
68379 | 28087 | glycogen | - | fermentation |
enzymes
@ref | value | activity | ec |
---|---|---|---|
30312 | catalase | + | 1.11.1.6 |
68379 | gelatinase | + | |
68379 | urease | - | 3.5.1.5 |
68379 | beta-glucosidase | + | 3.2.1.21 |
68379 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68379 | alpha-glucosidase | + | 3.2.1.20 |
68379 | beta-galactosidase | + | 3.2.1.23 |
68379 | beta-glucuronidase | - | 3.2.1.31 |
68379 | alkaline phosphatase | + | 3.1.3.1 |
68379 | pyrrolidonyl arylamidase | + | 3.4.19.3 |
68379 | pyrazinamidase | - | 3.5.1.B15 |
68382 | alkaline phosphatase | + | 3.1.3.1 |
68382 | lipase (C 14) | - | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | valine arylamidase | + | |
68382 | cystine arylamidase | + | 3.4.11.3 |
68382 | trypsin | - | 3.4.21.4 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | beta-galactosidase | + | 3.2.1.23 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | alpha-glucosidase | + | 3.2.1.20 |
68382 | beta-glucosidase | + | 3.2.1.21 |
68382 | N-acetyl-beta-glucosaminidase | + | 3.2.1.52 |
68382 | alpha-mannosidase | + | 3.2.1.24 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
API coryne
@ref | NIT | PYZ | PYRA | PAL | beta GUR | beta GAL | alpha GLU | beta NAG | ESC | URE | GEL | GLU | RIB | XYL | MAN | MAL | LAC | SAC | GLYG |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
69309 | - | - | + | + | - | + | + | - | + | - | + | - | - | - | - | - | - | - | - |
API zym
@ref | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
69309 | + | +/- | +/- | - | + | + | + | - | - | + | + | +/- | + | - | + | + | + | + | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | host species | geographic location | country | origin.country | continent |
---|---|---|---|---|---|---|
19156 | surface sterilized root of Eucalyptus microcarpa | Eucalyptus microcarpa | Adelaide, Bedford Park | Australia | AUS | Australia and Oceania |
67770 | Root of Eucalyptus microcarpa | Eucalyptus microcarpa | campus of Flinders Univ., Adelaide, South Australia | Australia | AUS | Australia and Oceania |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Host | #Plants | #Tree |
#Host Body-Site | #Plant | #Root (Rhizome) |
#Host Body-Site | #Plant | #Sterilized plant part |
Safety information
risk assessment
- @ref: 19156
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
19156 | Promicromonospora endophytica strain EUM 273 16S ribosomal RNA gene, partial sequence | GU434253 | 1370 | ena | 722703 |
67770 | Promicromonospora endophytica gene for 16S ribosomal RNA, partial sequence, strain: JCM 19560 | AB923928 | 1465 | ena | 722703 |
GC content
@ref | GC-content | method |
---|---|---|
19156 | 71.7 | high performance liquid chromatography (HPLC) |
30312 | 71.7 |
External links
@ref: 19156
culture collection no.: DSM 23716, JCM 19560, NRRL B-24816, EUM 273
straininfo link
- @ref: 87429
- straininfo: 408386
literature
- topic: Phylogeny
- Pubmed-ID: 21890721
- title: Promicromonospora endophytica sp. nov., an endophytic actinobacterium isolated from the root of an Australian native Grey Box tree.
- authors: Kaewkla O, Franco CMM
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.033258-0
- year: 2011
- mesh: Actinomycetales/*classification/genetics/*isolation & purification/physiology, Aerobiosis, Bacterial Typing Techniques, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Eucalyptus/*microbiology, Fatty Acids/analysis, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, Plant Roots/microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analysis
- topic2: Genetics
Reference
@id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed |
---|---|---|---|---|---|---|
19156 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23716) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-23716 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
30312 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 26653 | 28776041 | |
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
68379 | Automatically annotated from API Coryne | |||||
68382 | Automatically annotated from API zym | |||||
69309 | Wink, J. | https://cdn.dsmz.de/wink/DSM%2023716.pdf | Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig | |||
87429 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID408386.1 | StrainInfo: A central database for resolving microbial strain identifiers |