Cite this strain

"Anaerocellum danielii" (DSM 8977, Wai35.B1, KCTC 25802)

BacDive ID 18030

Type strain

Strain Designation Wai35.B1

Culture col. no. DSM 8977 Wai35.B1 KCTC 25802 NBRC 117004

NCBI tax ID(s) 1387557

Special Collections DSMZ

History <- F. A. Rainey, University of Queensland, Department of Microbiology, Brisbane, Australia; Wai35.B1

"Anaerocellum danielii" Wai35.B1 is an anaerobe bacterium that was isolated from hot spring.

anaerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

Culture Collection Numbers

DSM 8977 Wai35.B1 KCTC 25802 NBRC 117004

@ref 20215

Last LPSN update 2026-02-09 11:22:22

Domain Bacteria

Phylum Bacillota

Class Clostridia

Order Eubacteriales

Family [Eubacteriales, not assigned to family]

Genus "Anaerocellum"

Species "Anaerocellum danielii"

Full scientific name Anaerocellum danielii (Lee et al. 2018) Bing 2024

Synonyms (2)

"Anaerocellum danielii"
"Caldicellulosiruptor danielii"

Morphology

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
3449	CALDICELLULOSIRUPTOR MEDIUM (DSMZ Medium 640)		Medium recipe at MediaDive	Name: CALDICELLULOSIRUPTOR MEDIUM (DSMZ Medium 640) Composition: Trypticase peptone 2.0 g/l K2HPO4 1.5 g/l Cellobiose 1.0 g/l Yeast extract 1.0 g/l NH4Cl 0.9 g/l NaCl 0.9 g/l L-Cysteine HCl x H2O 0.75 g/l KH2PO4 0.75 g/l MgCl2 x 6 H2O 0.4 g/l HCl 0.0025 g/l FeCl3 x 6 H2O 0.0025 g/l FeCl2 x 4 H2O 0.0015 g/l Sodium resazurin 0.0005 g/l CoCl2 x 6 H2O 0.00019 g/l MnCl2 x 4 H2O 0.0001 g/l ZnCl2 7e-05 g/l Na2MoO4 x 2 H2O 3.6e-05 g/l NiCl2 x 6 H2O 2.4e-05 g/l H3BO3 6e-06 g/l CuCl2 x 2 H2O 2e-06 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
3449	positive	growth	65

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
3449	anaerobe
125438	anaerobe	96.254
125439	anaerobe	99.5

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Thermal spring
#Condition	#Thermophilic (>45°C)	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
3449	hot spring	Waimangu	New Zealand	NZL	Australia and Oceania

Taxonmaps

69479

Global distribution of 16S sequence L09182 (>99% sequence identity) for Caldicellulosiruptor from Microbeatlas

Aquatic Samples	3
Soil Samples	3
Animal Samples
Plant Samples
Total Samples	6

Interaction and safety

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM95572v1 assembly for Anaerocellum danielii Wai35.B1	contig	GCA_000955725	1387557.3	2558860987	1387557	78.94

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
3449	Gram-positive bacterial sp. (strain Wai35 B1) 16S ribosomal RNA (16S rRNA) gene	L09182	1527		1387557

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Caldicellulosiruptor sp. Wai35.B1
PATRIC: 1387557.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	72.10	no
125439	motility	BacteriaNetⓘ	yes	76.90	no
125439	gram_stain	BacteriaNetⓘ	variable	79.20	no
125439	oxygen_tolerance	BacteriaNetⓘ	anaerobe	99.50	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Caldicellulosiruptor sp. Wai35.B1
PATRIC: 1387557.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	70.11	no
125438	anaerobic	anaerobicⓘ	yes	96.25	yes
125438	spore-forming	spore-formingⓘ	no	54.80	no
125438	aerobic	aerobicⓘ	no	97.35	yes
125438	thermophilic	thermophileⓘ	yes	83.77	yes
125438	flagellated	motile2+ⓘ	yes	72.50	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	Genus-Wide Assessment of Lignocellulose Utilization in the Extremely Thermophilic Genus Caldicellulosiruptor by Genomic, Pangenomic, and Metagenomic Analyses.	Lee LL, Blumer-Schuette SE, Izquierdo JA, Zurawski JV, Loder AJ, Conway JM, Elkins JG, Podar M, Clum A, Jones PC, Piatek MJ, Weighill DA, Jacobson DA, Adams MWW, Kelly RM	Appl Environ Microbiol	10.1128/AEM.02694-17	2018
Genetics	Complete Genome Sequences of Caldicellulosiruptor sp. Strain Rt8.B8, Caldicellulosiruptor sp. Strain Wai35.B1, and "Thermoanaerobacter cellulolyticus".	Lee LL, Izquierdo JA, Blumer-Schuette SE, Zurawski JV, Conway JM, Cottingham RW, Huntemann M, Copeland A, Chen IM, Kyrpides N, Markowitz V, Palaniappan K, Ivanova N, Mikhailova N, Ovchinnikova G, Andersen E, Pati A, Stamatis D, Reddy TB, Shapiro N, Nordberg HP, Cantor MN, Hua SX, Woyke T, Kelly RM	Genome Announc	10.1128/genomeA.00440-15	2015

References

#3449	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 8977
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .

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"Anaerocellum danielii" (DSM 8977, Wai35.B1, KCTC 25802)

Name and taxonomic classification

Culture Collection Numbers

Morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Sequence information

Genome sequences

Genome browser: GCA_000955725

16S sequences

Genome-based predictions

Predictions

Literature

Literature

References

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