Strain identifier
BacDive ID: 18030
Type strain: 
Species: Caldicellulosiruptor sp.
Strain Designation: Wai35.B1
Strain history: <- F. A. Rainey, University of Queensland, Department of Microbiology, Brisbane, Australia; Wai35.B1
NCBI tax ID(s): 1981791 (species)
version 9.1 (current version)
General
@ref: 3449
BacDive-ID: 18030
DSM-Number: 8977
keywords: genome sequence, 16S sequence, Bacteria, anaerobe
description: Caldicellulosiruptor sp. Wai35.B1 is an anaerobe bacterium that was isolated from hot spring.
NCBI tax id
- NCBI tax id: 1981791
- Matching level: species
strain history
- @ref: 3449
- history: <- F. A. Rainey, University of Queensland, Department of Microbiology, Brisbane, Australia; Wai35.B1
doi: 10.13145/bacdive18030.20240916.9.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Clostridia
- order: Eubacteriales
- family: Syntrophomonadaceae
- genus: Caldicellulosiruptor
- species: Caldicellulosiruptor sp.
- full scientific name: Caldicellulosiruptor Rainey et al. 1995
@ref: 3449
domain: Bacteria
phylum: Firmicutes
class: Clostridia
order: Clostridiales
family: Clostridia, not assigned to family
genus: Caldicellulosiruptor
species: Caldicellulosiruptor sp.
full scientific name: Caldicellulosiruptor sp.
strain designation: Wai35.B1
type strain: no
Culture and growth conditions
culture medium
- @ref: 3449
- name: CALDICELLULOSIRUPTOR MEDIUM (DSMZ Medium 640)
- growth: yes
- link: https://mediadive.dsmz.de/medium/640
- composition: Name: CALDICELLULOSIRUPTOR MEDIUM (DSMZ Medium 640) Composition: Trypticase peptone 2.0 g/l K2HPO4 1.5 g/l Cellobiose 1.0 g/l Yeast extract 1.0 g/l NH4Cl 0.9 g/l NaCl 0.9 g/l L-Cysteine HCl x H2O 0.75 g/l KH2PO4 0.75 g/l MgCl2 x 6 H2O 0.4 g/l HCl 0.0025 g/l FeCl3 x 6 H2O 0.0025 g/l FeCl2 x 4 H2O 0.0015 g/l Sodium resazurin 0.0005 g/l CoCl2 x 6 H2O 0.00019 g/l MnCl2 x 4 H2O 0.0001 g/l ZnCl2 7e-05 g/l Na2MoO4 x 2 H2O 3.6e-05 g/l NiCl2 x 6 H2O 2.4e-05 g/l H3BO3 6e-06 g/l CuCl2 x 2 H2O 2e-06 g/l Distilled water
culture temp
- @ref: 3449
- growth: positive
- type: growth
- temperature: 65
Physiology and metabolism
oxygen tolerance
| @ref | oxygen tolerance | confidence |
|---|---|---|
| 3449 | anaerobe | |
| 69480 | anaerobe | 96.254 |
Isolation, sampling and environmental information
isolation
- @ref: 3449
- sample type: hot spring
- geographic location: Waimangu
- country: New Zealand
- origin.country: NZL
- continent: Australia and Oceania
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Aquatic | #Thermal spring |
| #Condition | #Thermophilic (>45°C) |
taxonmaps
- @ref: 69479
- File name: preview.99_3874.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_153;96_509;97_2389;98_2951;99_3874&stattab=map
- Last taxonomy: Caldicellulosiruptor
- 16S sequence: L09182
- Sequence Identity:
- Total samples: 6
- soil counts: 3
- aquatic counts: 3
Sequence information
16S sequences
- @ref: 3449
- description: Gram-positive bacterial sp. (strain Wai35 B1) 16S ribosomal RNA (16S rRNA) gene
- accession: L09182
- length: 1527
- database: nuccore
- NCBI tax ID: 1387557
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Caldicellulosiruptor sp. Wai35.B1 | 1387557.3 | complete | patric | 1387557 |
| 66792 | Caldicellulosiruptor danielii Wai35.B1, DSM 8977 | 2558860987 | draft | img | 1387557 |
Genome-based predictions
predictions
| @ref | trait | model | description | prediction | confidence | training_data |
|---|---|---|---|---|---|---|
| 69480 | gram-positive | gram-positive | Positive reaction to Gram-staining | yes | 70.109 | no |
| 69480 | anaerobic | anaerobic | Ability to grow under anoxygenic conditions (including facultative anaerobes) | yes | 96.254 | yes |
| 69480 | spore-forming | spore-forming | Ability to form endo- or exospores | no | 54.799 | no |
| 69480 | aerobic | aerobic | Ability to grow under oxygenic conditions (including facultative aerobes) | no | 97.352 | yes |
| 69480 | thermophilic | thermophile | Ability to grow at temperatures above or equal to 45°C | yes | 83.769 | yes |
| 69480 | flagellated | motile2+ | Ability to perform flagellated movement | yes | 72.5 | no |
External links
@ref: 3449
culture collection no.: DSM 8977
straininfo link
- @ref: 87019
- straininfo: 48553
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Genetics | 25977428 | Complete Genome Sequences of Caldicellulosiruptor sp. Strain Rt8.B8, Caldicellulosiruptor sp. Strain Wai35.B1, and "Thermoanaerobacter cellulolyticus". | Lee LL, Izquierdo JA, Blumer-Schuette SE, Zurawski JV, Conway JM, Cottingham RW, Huntemann M, Copeland A, Chen IM, Kyrpides N, Markowitz V, Palaniappan K, Ivanova N, Mikhailova N, Ovchinnikova G, Andersen E, Pati A, Stamatis D, Reddy TB, Shapiro N, Nordberg HP, Cantor MN, Hua SX, Woyke T, Kelly RM | Genome Announc | 10.1128/genomeA.00440-15 | 2015 | ||
| Genetics | 29475869 | Genus-Wide Assessment of Lignocellulose Utilization in the Extremely Thermophilic Genus Caldicellulosiruptor by Genomic, Pangenomic, and Metagenomic Analyses. | Lee LL, Blumer-Schuette SE, Izquierdo JA, Zurawski JV, Loder AJ, Conway JM, Elkins JG, Podar M, Clum A, Jones PC, Piatek MJ, Weighill DA, Jacobson DA, Adams MWW, Kelly RM | Appl Environ Microbiol | 10.1128/AEM.02694-17 | 2018 | Cellulose/metabolism, Firmicutes/classification/*genetics/*metabolism, *Genome, Bacterial, Genomics, Lignin/*metabolism, *Metagenome, Metagenomics | Metabolism |
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 3449 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 8977) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-8977 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
| 87019 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID48553.1 | StrainInfo: A central database for resolving microbial strain identifiers |