Name: Bifidobacterium pseudolongum PNC-2-9G (Biotype a)
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 1717

Type strain:

Species: Bifidobacterium pseudolongum

Strain Designation: PNC-2-9G (Biotype a), PNC-2-9G

Culture col. no.: DSM 20099, ATCC 25526, CCUG 34981, CCM 7207, JCM 1205, BCRC 14673, CGMCC 1.2185, CIP 105352, KCTC 3224, LMG 11571, NCFB 2244

Strain history: CIP <- 1998, ATCC <- T. Mitsuoka: strain PNC-2-9G

NCBI tax ID(s): 1694 (species)

SI-ID 9647

LMG 11571, ATCC 25526, CCUG 34981, DSM 20099, NCFB 2244, CIP 105352, JCM 1205, CCRC 14673, NCIMB 702244, NCDO2244, KCTC 3224, AS 1.2185, CCM 7207, BCRC 14673, HAMBI 113, CGMCC 1.2185, VTT E-021913

Special Collections

Other strains from Bifidobacterium pseudolongum

Section

Bifidobacterium pseudolongum PNC-2-9G is an anaerobe, Gram-positive, rod-shaped bacterium that was isolated from pig faeces.

Gram-positive
rod-shaped
anaerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Bifidobacteriales*
	Family	*Bifidobacteriaceae*
	Genus	*Bifidobacterium*
	Species	**Bifidobacterium pseudolongum**
	Full Scientific Name (LPSN)	Bifidobacterium pseudolongum Mitsuoka 1969 (Approved Lists 1980)

Information on morphological and physiological properties Morphology

[Ref.: #121991]	Gram stain	positive

[Ref.: #121991]	Cell shape	rod-shaped

[Ref.: #121991]	Motility	no
[Ref.: #69480]	Motility	no Confidence in %94

Information on culture and growth conditions Culture and growth conditions

[Ref.: #8525]

Culture medium

BIFIDOBACTERIUM MEDIUM (DSMZ Medium 58)

[Ref.: #8525]

Culture medium growth

[Ref.: #8525]

Culture medium link

Medium recipe at MediaDive

[Ref.: #8525]

Culture medium composition

expand / minimize

[Ref.: #35803]

Culture medium

MEDIUM 20 - for Anaerobic bacteria

[Ref.: #35803]

Culture medium growth

[Ref.: #35803]

Culture medium composition

expand / minimize

[Ref.: #121991]

Culture medium

CIP Medium 20

[Ref.: #121991]

Culture medium growth

[Ref.: #121991]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #8525]	growth	37 °C
[Ref.: #35803]	growth	37 °C
[Ref.: #52431]	growth	37 °C
[Ref.: #67770]	growth	37 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #8525]	Oxygen tolerance	anaerobe
[Ref.: #52431]	Oxygen tolerance	anaerobe

[Ref.: #8525]	Murein short key	A21.02
[Ref.: #8525]	Murein types	A3ß L-Orn(L-Lys)-L-Ala₂_-₃

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	amygdalin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	arbutin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	cellobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-galactose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-glucose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-ribose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-xylose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #121991]	esculin ChEBI	+	hydrolysis
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gentiobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycerol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycogen ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	lactose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	maltose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	N-acetylglucosamine ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #121991]	nitrate ChEBI	-	reduction
[Ref.: #121991]	nitrate ChEBI	+	respiration
[Ref.: #121991]	nitrite ChEBI	-	reduction
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	raffinose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	salicin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	starch ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	sucrose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	trehalose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	turanose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #121991]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	+	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #121991]	amylase	+
[Ref.: #121991]	beta-galactosidase	-	3.2.1.23
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #121991]	caseinase	-	3.4.21.50
[Ref.: #121991]	catalase	-	1.11.1.6
[Ref.: #68382]	cystine arylamidase	+	3.4.11.3	* from API zym
[Ref.: #121991]	DNase	-
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #121991]	gelatinase	-
[Ref.: #121991]	lecithinase	-
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #121991]	lipase	-
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #121991]	oxidase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #121991]	tween esterase	-
[Ref.: #121991]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #52431]

Fatty acid	Percentage	ECL
C16:0	31.6	16
C14:0	18.3	14
C18:1 ω9c	15.3	17.769
Unidentified	8.8	19.321
C18:2 ω6,9c/C18:0 ANTE	6.3	17.724
C16:1 ω9c	4.3	15.774
C12:0	3.9	12
C18:0	3.1	18
C13:0 3OH/C15:1 i I/H	2.8	14.469
C18:1 ω6c	1.6	17.858
C19:0 CYCLO ω9c	1.5	18.87
C17:0 iso 3OH	1.3	18.161
C17:1 ω5c ISO	1.2	16.461

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #8525]	14526	-	-	-	-	+	+	+	-	-	-	+	+	+	-	-	-	-	-	-	-	-	+	-	-	-	+	-	-	+	+	+	+	-	-	-	+	+	+	-	-	+	-	-	-	-	-	-	-	-	-
[Ref.: #121991]	10052	not determinedn.d.	-	-	-	+	+	+	-	-	-	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	+	+	+	-	-	-	+	+	+	-	-	+	-	-	-	-	-	-	-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #121991]	8640	-	-	+	+	-	+	-	+	-	-	+	+	+	+	-	+	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #8525]	Sample type/isolated from	pig faeces

[Ref.: #52431]	Sample type/isolated from	Pig feces

[Ref.: #67770]	Sample type/isolated from	Swine feces
* marker position based on {}

Isolation sources categories

#Host	#Mammals	#Suidae (Pig,Swine)
#Host Body Product	#Gastrointestinal tract	#Feces (Stool)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence LC071797 (>99% sequence identity) for Bifidobacterium pseudolongum subclade from Microbeatlas

Aquatic Samples	229
Soil Samples	398
Animal Samples	31773
Plant Samples	137
Total Samples	32537

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #8525]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #121991]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Bifidobacterium pseudolongum subsp. pseudolongum gene for 16S ribosomal RNA, partial sequence, strain: JCM 1205	LC071797	1404	GenBank ENA	31954 tax ID
[Ref.: #20218]	Bifidobacterium pseudolongum 16S ribosomal RNA	M58742	1475	GenBank ENA	1694 tax ID	*
[Ref.: #20218]	Bifidobacterium pseudolongum ATCC 25526 16S rRNA-23S rRNA intergenic spacer	U09879	541	GenBank ENA	1694 tax ID	*
[Ref.: #20218]	Bifidobacterium pseudolongum subsp. pseudolongum strain DSM 20099 16S ribosomal RNA gene, partial sequence	GQ487642	151	GenBank ENA	31954 tax ID	*
[Ref.: #20218]	Bifidobacterium pseudolongum subsp. pseudolongum gene for 16S rRNA, partial sequence, strain: JCM 1205	AB116335	472	GenBank ENA	31954 tax ID	*
[Ref.: #20218]	Bifidobacterium pseudolongum subsp. pseudolongum gene for 16S rRNA, partial sequence, strain: JCM 1205	AB507141	671	GenBank ENA	31954 tax ID	*
[Ref.: #20218]	Bifidobacterium pseudolongum subsp. pseudolongum JCM 1205 gene for 16S rRNA, partial sequence	D86195	1527	GenBank ENA	31954 tax ID	*
[Ref.: #20218]	Bifidobacterium pseudolongum subsp. pseudolongum strain KCTC 3224 16S ribosomal RNA gene, partial sequence	GU361829	1490	GenBank ENA	31954 tax ID	*
[Ref.: #20218]	Bifidobacterium pseudolongum subsp. pseudolongum strain LMG11571 16S ribosomal RNA gene and 16S-23S ribosomal RNA intergenic spacer, partial sequence	FJ231745	812	GenBank ENA	31954 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Bifidobacterium pseudolongum subsp. pseudolongum DSM 20099	GCA_000771225	contig	GenBank ENA	1437595 tax ID
[Ref.: #67770]	Bifidobacterium pseudolongum subsp. pseudolongum LMG 11571	GCA_000741325	contig	GenBank ENA	31954 tax ID
[Ref.: #66792]	Bifidobacterium pseudolongum subsp. pseudolongum DSM 20099	1437595.3	wgs	PATRIC	1437595 tax ID	*
[Ref.: #66792]	Bifidobacterium pseudolongum subsp. pseudolongum strain LMG 11571	31954.3	wgs	PATRIC	31954 tax ID	*
[Ref.: #66792]	Bifidobacterium pseudolongum pseudolongum LMG 11571	2597490262	draft	JGI IMG/M	31954 tax ID	*
[Ref.: #66792]	Bifidobacterium pseudolongum pseudolongum DSM 20099	2663763404	draft	JGI IMG/M	1437595 tax ID	*

[Ref.: #8525]	GC-content	59.5 mol%
[Ref.: #67770]	GC-content	59.5 mol%	thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Bifidobacterium pseudolongum subsp. pseudolongum ATCC 25526 NCBI: GCA_022691205.1
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	87.121	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	yes	83.396	no
[Ref.: #69480]	aerobic	aerobicⓘ	no	94.589	no
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	89.777	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	94	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	no	94	no

Availability in culture collections External links

[Ref.: #8525]

Culture collection no.

DSM 20099, ATCC 25526, CCUG 34981, CCM 7207, JCM 1205, BCRC 14673, CGMCC 1.2185, CIP 105352, KCTC 3224, LMG 11571, NCFB 2244

[Ref.: #71364]

SI-ID 9647

Literature:

	Title	Authors	Journal	DOI	Year
Biotechnology	Adhesion of bifidobacteria to granular starch and its implications in probiotic technologies.	Crittenden R, Laitila A, Forssell P, Matto J, Saarela M, Mattila-Sandholm T, Myllarinen P	Appl Environ Microbiol	10.1128/AEM.67.8.3469-3475.2001	2001	*
	Investigation of Antibacterial Activity and Probiotic Properties of Strains Belonging to Lactobacillus and Bifidobacterium Genera for Their Potential Application in Functional Food and Feed Products.	Cizeikiene D, Jagelaviciute J	Probiotics Antimicrob Proteins	10.1007/s12602-021-09777-5	2021	*

References

#8525

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 20099

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#35803 ; Curators of the CIP;
#52431 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 34981

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68371

Automatically annotated from API 50CH acid .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#71364

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID9647.1 )

#121991 Collection of Institut Pasteur ; Curators of the CIP; CIP 105352
* These data were automatically processed and therefore are not curated

Change proposal

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Bifidobacterium pseudolongum subsp. pseudolongum ATCC 25526

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Change proposal