Thermoproteus tenax (DSM 2078, ATCC 35583, JCM 9277)

Name: Thermoproteus tenax (DSM 2078, ATCC 35583, JCM 9277)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 17031

Type strain

Culture col. no. DSM 2078 ATCC 35583 JCM 9277 Kra 1 NBRC 100435

NCBI tax ID(s) 768679 2271

Special Collections DSMZ JCM

Links

Thermoproteus tenax DSM 2078 is an anaerobe, hyperthermophilic prokaryote that was isolated from mud hole, solfataric field.

anaerobe hyperthermophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 41172

ATCC 35583,DSM 2078,JCM 9277,NBRC 100435

@ref 20215

Last LPSN update 2025-12-16 09:50:59

Domain Thermoproteati

Phylum Thermoproteota

Class Thermoprotei

Order Thermoproteales

Family Thermoproteaceae

Genus Thermoproteus

Species Thermoproteus tenax

Full scientific name Thermoproteus tenax Zillig and Stetter 1982

Morphology

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
713	THERMOPROTEUS MEDIUM (DSMZ Medium 185)		Medium recipe at MediaDive	Name: THERMOPROTEUS MEDIUM (DSMZ Medium 185) Composition: Sulfur 10.0 g/l Starch 5.0 g/l FeSO4 x 7 H2O 0.556 g/l Na2S x 9 H2O 0.5 g/l MgSO4 x 7 H2O 0.492 g/l CaSO4 x 2 H2O 0.344 g/l (NH4)2SO4 0.264 g/l Yeast extract 0.2 g/l KH2PO4 0.014 g/l Resazurin 0.001 g/l NaF 0.00084 g/l Na2B4O7 x 10 H2O 0.00045 g/l MnCl2 x 4 H2O 0.00018 g/l ZnSO4 x 7 H2O 2.2e-05 g/l CuCl2 x 2 H2O 5e-06 g/l Na2MoO4 x 2 H2O 3e-06 g/l CoSO4 x 7 H2O 1e-06 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
713	positive	growth	85	hyperthermophilic
67770	positive	growth	85	hyperthermophilic

Physiology and metabolism

Oxygen tolerance

713

Oxygen toleranceanaerobe

Observation

67770

Observationquinones: MK-6(H₁₂), MK-5(H₁₀), MK-4(H₈), MK-6(H₁₀), MK-5(H₈), MK-4(H₆), MMK-6(H₁₂), MMK-5(H₁₀)

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	formaldehyde oxidation	100	3 of 3
66794	teichoic acid biosynthesis	100	1 of 1
66794	valine metabolism	100	9 of 9
66794	methylglyoxal degradation	100	5 of 5
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	coenzyme A metabolism	100	4 of 4
66794	glycogen metabolism	100	5 of 5
66794	adipate degradation	100	2 of 2
66794	suberin monomers biosynthesis	100	2 of 2
66794	ribulose monophosphate pathway	100	2 of 2
66794	molybdenum cofactor biosynthesis	88.89	8 of 9
66794	aspartate and asparagine metabolism	88.89	8 of 9
66794	chorismate metabolism	88.89	8 of 9
66794	C4 and CAM-carbon fixation	87.5	7 of 8
66794	flavin biosynthesis	86.67	13 of 15
66794	starch degradation	80	8 of 10
66794	glutamate and glutamine metabolism	78.57	22 of 28
66794	citric acid cycle	78.57	11 of 14
66794	photosynthesis	78.57	11 of 14
66794	d-mannose degradation	77.78	7 of 9
66794	CO2 fixation in Crenarchaeota	77.78	7 of 9
66794	palmitate biosynthesis	77.27	17 of 22
66794	vitamin B12 metabolism	76.47	26 of 34
66794	glycolysis	76.47	13 of 17
66794	alanine metabolism	75.86	22 of 29
66794	acetate fermentation	75	3 of 4
66794	isoleucine metabolism	75	6 of 8
66794	glycogen biosynthesis	75	3 of 4
66794	lactate fermentation	75	3 of 4
66794	pentose phosphate pathway	72.73	8 of 11
66794	NAD metabolism	72.22	13 of 18
66794	reductive acetyl coenzyme A pathway	71.43	5 of 7
66794	propanol degradation	71.43	5 of 7
66794	purine metabolism	71.28	67 of 94
66794	Entner Doudoroff pathway	70	7 of 10
66794	threonine metabolism	70	7 of 10
66794	vitamin B1 metabolism	69.23	9 of 13
66794	phenylalanine metabolism	69.23	9 of 13
66794	pyrimidine metabolism	68.89	31 of 45
66794	cyanate degradation	66.67	2 of 3
66794	acetoin degradation	66.67	2 of 3
66794	methane metabolism	66.67	2 of 3
66794	pantothenate biosynthesis	66.67	4 of 6
66794	serine metabolism	66.67	6 of 9
66794	L-lactaldehyde degradation	66.67	2 of 3
66794	heme metabolism	64.29	9 of 14
66794	lysine metabolism	64.29	27 of 42
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	62.5	5 of 8
66794	gluconeogenesis	62.5	5 of 8
66794	leucine metabolism	61.54	8 of 13
66794	methionine metabolism	61.54	16 of 26
66794	cellulose degradation	60	3 of 5
66794	phenylacetate degradation (aerobic)	60	3 of 5
66794	4-hydroxyphenylacetate degradation	60	6 of 10
66794	propionate fermentation	60	6 of 10
66794	polyamine pathway	56.52	13 of 23
66794	proline metabolism	54.55	6 of 11
66794	sulfate reduction	53.85	7 of 13
66794	urea cycle	53.85	7 of 13
66794	tryptophan metabolism	52.63	20 of 38
66794	sulfopterin metabolism	50	2 of 4
66794	glycolate and glyoxylate degradation	50	3 of 6
66794	1,4-dihydroxy-6-naphthoate biosynthesis	50	3 of 6
66794	toluene degradation	50	2 of 4
66794	aminopropanol phosphate biosynthesis	50	1 of 2
66794	dolichol and dolichyl phosphate biosynthesis	50	1 of 2
66794	CMP-KDO biosynthesis	50	2 of 4
66794	dTDPLrhamnose biosynthesis	50	4 of 8
66794	CDP-diacylglycerol biosynthesis	50	1 of 2
66794	ethanol fermentation	50	1 of 2
66794	degradation of aromatic, nitrogen containing compounds	50	6 of 12
66794	ketogluconate metabolism	50	4 of 8
66794	phenylmercury acetate degradation	50	1 of 2
66794	butanoate fermentation	50	2 of 4
66794	tetrahydrofolate metabolism	50	7 of 14
66794	cis-vaccenate biosynthesis	50	1 of 2
66794	selenocysteine biosynthesis	50	3 of 6
66794	cysteine metabolism	50	9 of 18
66794	glycine metabolism	50	5 of 10
66794	isoprenoid biosynthesis	50	13 of 26
66794	histidine metabolism	48.28	14 of 29
66794	oxidative phosphorylation	47.25	43 of 91
66794	arginine metabolism	45.83	11 of 24
66794	metabolism of disaccharids	45.45	5 of 11
66794	d-xylose degradation	45.45	5 of 11
66794	lipid metabolism	45.16	14 of 31
66794	lipid A biosynthesis	44.44	4 of 9
66794	degradation of sugar alcohols	43.75	7 of 16
66794	ubiquinone biosynthesis	42.86	3 of 7
66794	tyrosine metabolism	42.86	6 of 14
66794	glutathione metabolism	42.86	6 of 14
66794	bacilysin biosynthesis	40	2 of 5
66794	glycine betaine biosynthesis	40	2 of 5
66794	coenzyme M biosynthesis	40	4 of 10
66794	hydrogen production	40	2 of 5
66794	ethylmalonyl-CoA pathway	40	2 of 5
66794	myo-inositol biosynthesis	40	4 of 10
66794	creatinine degradation	40	2 of 5
66794	degradation of pentoses	39.29	11 of 28
66794	degradation of hexoses	38.89	7 of 18
66794	dolichyl-diphosphooligosaccharide biosynthesis	36.36	4 of 11
66794	degradation of sugar acids	36	9 of 25
66794	sphingosine metabolism	33.33	2 of 6
66794	IAA biosynthesis	33.33	1 of 3
66794	peptidoglycan biosynthesis	33.33	5 of 15
66794	octane oxidation	33.33	1 of 3
66794	acetyl CoA biosynthesis	33.33	1 of 3
66794	methanogenesis from CO2	33.33	4 of 12
66794	enterobactin biosynthesis	33.33	1 of 3
66794	non-pathway related	31.58	12 of 38
66794	phenol degradation	30	6 of 20
66794	bile acid biosynthesis, neutral pathway	29.41	5 of 17
66794	mevalonate metabolism	28.57	2 of 7
66794	vitamin B6 metabolism	27.27	3 of 11
66794	cyclohexanol degradation	25	1 of 4
66794	carnitine metabolism	25	2 of 8
66794	nitrate assimilation	22.22	2 of 9

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Mud (Sludge)
#Environmental	#Terrestrial	#Volcanic

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
713	mud hole, solfataric field	Krafta	Iceland	ISL	Europe
67770	Acidic solfataric hot spring	Krafla	Iceland	ISL	Europe

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
713	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM25305v1 assembly for Thermoproteus tenax Kra 1	complete	GCA_000253055	768679.9	2511231093	768679	98.77

Genome browser: GCA_000253055

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
713	T.tenax 16S rRNA	M35966.1	1504		2271

GC content

@ref	GC-content (mol%)
713	55.5
67770	55.9
67770	55.3

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Circularization restores signal recognition particle RNA functionality in Thermoproteus.	Plagens A, Daume M, Wiegel J, Randau L.	Elife	10.7554/elife.11623	2015
Metabolism	Characterization of the CRISPR/Cas subtype I-A system of the hyperthermophilic crenarchaeon Thermoproteus tenax.	Plagens A, Tjaden B, Hagemann A, Randau L, Hensel R.	J Bacteriol	10.1128/jb.00206-12	2012
	The first prokaryotic trehalose synthase complex identified in the hyperthermophilic crenarchaeon Thermoproteus tenax.	Zaparty M, Hagemann A, Brasen C, Hensel R, Lupas AN, Brinkmann H, Siebers B.	PLoS One	10.1371/journal.pone.0061354	2013
Metabolism	DNA microarray analysis of central carbohydrate metabolism: glycolytic/gluconeogenic carbon switch in the hyperthermophilic crenarchaeum Thermoproteus tenax.	Zaparty M, Zaigler A, Stamme C, Soppa J, Hensel R, Siebers B.	J Bacteriol	10.1128/jb.01524-07	2008
	Complete genome sequence of Pyrobaculum oguniense.	Bernick DL, Karplus K, Lui LM, Coker JK, Murphy JN, Chan PP, Cozen AE, Lowe TM.	Stand Genomic Sci	10.4056/sigs.2645906	2012
Enzymology	Glycerate kinase of the hyperthermophilic archaeon Thermoproteus tenax: new insights into the phylogenetic distribution and physiological role of members of the three different glycerate kinase classes.	Kehrer D, Ahmed H, Brinkmann H, Siebers B.	BMC Genomics	10.1186/1471-2164-8-301	2007
Metabolism	In vitro assembly and activity of an archaeal CRISPR-Cas type I-A Cascade interference complex.	Plagens A, Tripp V, Daume M, Sharma K, Klingl A, Hrle A, Conti E, Urlaub H, Randau L.	Nucleic Acids Res	10.1093/nar/gku120	2014
Metabolism	CC1, a novel crenarchaeal DNA binding protein.	Luo X, Schwarz-Linek U, Botting CH, Hensel R, Siebers B, White MF.	J Bacteriol	10.1128/jb.01246-06	2007
Enzymology	PPi-dependent phosphofructokinase from Thermoproteus tenax, an archaeal descendant of an ancient line in phosphofructokinase evolution.	Siebers B, Klenk HP, Hensel R.	J Bacteriol	10.1128/jb.180.8.2137-2143.1998	1998
Enzymology	Pyruvate kinase of the hyperthermophilic crenarchaeote Thermoproteus tenax: physiological role and phylogenetic aspects.	Schramm A, Siebers B, Tjaden B, Brinkmann H, Hensel R.	J Bacteriol	10.1128/jb.182.7.2001-2009.2000	2000
	Organic solutes in hyperthermophilic archaea.	Martins LO, Huber R, Huber H, Stetter KO, Da Costa MS, Santos H.	Appl Environ Microbiol	10.1128/aem.63.3.896-902.1997	1997
Enzymology	Reconstruction of the central carbohydrate metabolism of Thermoproteus tenax by use of genomic and biochemical data.	Siebers B, Tjaden B, Michalke K, Dorr C, Ahmed H, Zaparty M, Gordon P, Sensen CW, Zibat A, Klenk HP, Schuster SC, Hensel R.	J Bacteriol	10.1128/jb.186.7.2179-2194.2004	2004
	The cell envelope of Thermoproteus tenax: three-dimensional structure of the surface layer and its role in shape maintenance.	Wildhaber I, Baumeister W.	EMBO J	10.1002/j.1460-2075.1987.tb02389.x	1987
Enzymology	The semi-phosphorylative Entner-Doudoroff pathway in hyperthermophilic archaea: a re-evaluation.	Ahmed H, Ettema TJ, Tjaden B, Geerling AC, van der Oost J, Siebers B.	Biochem J	10.1042/bj20041711	2005
Pathogenicity	Unique antibiotic sensitivity of archaebacterial polypeptide elongation factors.	Londei P, Sanz JL, Altamura S, Hummel H, Cammarano P, Amils R, Bock A, Wolf H.	J Bacteriol	10.1128/jb.167.1.265-271.1986	1986
	Ultrastructure of the cell envelope of the archaebacteria Thermoproteus tenax and Thermoproteus neutrophilus.	Messner P, Pum D, Sara M, Stetter KO, Sleytr UB.	J Bacteriol	10.1128/jb.166.3.1046-1054.1986	1986
Phylogeny	Discontinuous occurrence of the hsp70 (dnaK) gene among Archaea and sequence features of HSP70 suggest a novel outlook on phylogenies inferred from this protein.	Gribaldo S, Lumia V, Creti R, Conway de Macario E, Sanangelantoni A, Cammarano P.	J Bacteriol	10.1128/jb.181.2.434-443.1999	1999
Metabolism	Presence of acetyl coenzyme A (CoA) carboxylase and propionyl-CoA carboxylase in autotrophic Crenarchaeota and indication for operation of a 3-hydroxypropionate cycle in autotrophic carbon fixation.	Menendez C, Bauer Z, Huber H, Gad'on N, Stetter KO, Fuchs G.	J Bacteriol	10.1128/jb.181.4.1088-1098.1999	1999
Genetics	P finder: genomic and metagenomic annotation of RNase P RNA gene (rnpB).	Ellis JC.	BMC Genomics	10.1186/s12864-020-6615-z	2020
Phylogeny	A virus of hyperthermophilic archaea with a unique architecture among DNA viruses.	Rensen EI, Mochizuki T, Quemin E, Schouten S, Krupovic M, Prangishvili D.	Proc Natl Acad Sci U S A	10.1073/pnas.1518929113	2016
Genetics	Correcting Inconsistencies and Errors in Bacterial Genome Metadata Using an Automated Curation Tool in Excel (AutoCurE).	Schmedes SE, King JL, Budowle B.	Front Bioeng Biotechnol	10.3389/fbioe.2015.00138	2015
Phylogeny	Metagenomic Characterization of Candidatus Smithella cisternae Strain M82_1, a Syntrophic Alkane-Degrading Bacteria, Enriched from the Shengli Oil Field.	Qin QS, Feng DS, Liu PF, He Q, Li X, Liu AM, Zhang H, Hu GQ, Cheng L.	Microbes Environ	10.1264/jsme2.me17022	2017
Metabolism	Comparative genomics of molybdenum utilization in prokaryotes and eukaryotes.	Peng T, Xu Y, Zhang Y.	BMC Genomics	10.1186/s12864-018-5068-0	2018
Metabolism	Novel Transcriptional Regulons for Autotrophic Cycle Genes in Crenarchaeota.	Leyn SA, Rodionova IA, Li X, Rodionov DA.	J Bacteriol	10.1128/jb.00249-15	2015
	Phylogeny and Taxonomy of Archaea: A Comparison of the Whole-Genome-Based CVTree Approach with 16S rRNA Sequence Analysis.	Zuo G, Xu Z, Hao B.	Life (Basel)	10.3390/life5010949	2015
Enzymology	Candidatus Nitrosocaldus cavascurensis, an Ammonia Oxidizing, Extremely Thermophilic Archaeon with a Highly Mobile Genome.	Abby SS, Melcher M, Kerou M, Krupovic M, Stieglmeier M, Rossel C, Pfeifer K, Schleper C.	Front Microbiol	10.3389/fmicb.2018.00028	2018
Enzymology	Quinones from Archaebacteria, I. New types of menaquinones from the thermophilic archaebacterium Thermoproteus tenax.	Thurl S, Buhrow I, Schafer W.	Biol Chem Hoppe Seyler	10.1515/bchm3.1985.366.2.1079	1985
Phylogeny	Distribution of Crenarchaeota representatives in terrestrial hot springs of Russia and Iceland.	Perevalova AA, Kolganova TV, Birkeland NK, Schleper C, Bonch-Osmolovskaya EA, Lebedinsky AV.	Appl Environ Microbiol	10.1128/aem.00972-08	2008
Metabolism	Phylogenomic reconstruction of archaeal fatty acid metabolism.	Dibrova DV, Galperin MY, Mulkidjanian AY.	Environ Microbiol	10.1111/1462-2920.12359	2014
	Vitamin contents of archaebacteria.	Noll KM, Barber TS.	J Bacteriol	10.1128/jb.170.9.4315-4321.1988	1988
	Heavily Armed Ancestors: CRISPR Immunity and Applications in Archaea with a Comparative Analysis of CRISPR Types in Sulfolobales.	Zink IA, Wimmer E, Schleper C.	Biomolecules	10.3390/biom10111523	2020
	Characterization of the 7S RNA and its gene from halobacteria.	Moritz A, Goebel W.	Nucleic Acids Res	10.1093/nar/13.19.6969	1985
Genetics	The complete genome sequence of Thermoproteus tenax: a physiologically versatile member of the Crenarchaeota.	Siebers B, Zaparty M, Raddatz G, Tjaden B, Albers SV, Bell SD, Blombach F, Kletzin A, Kyrpides N, Lanz C, Plagens A, Rampp M, Rosinus A, von Jan M, Makarova KS, Klenk HP, Schuster SC, Hensel R	PLoS One	10.1371/journal.pone.0024222	2011
Enzymology	Identification and characterization of the genes encoding three structural proteins of the Thermoproteus tenax virus TTV1.	Neumann H, Schwass V, Eckerskorn C, Zillig W	Mol Gen Genet	10.1007/BF00330948	1989
Metabolism	Pyrobaculum aerophilum sp. nov., a novel nitrate-reducing hyperthermophilic archaeum.	Volkl P, Huber R, Drobner E, Rachel R, Burggraf S, Trincone A, Stetter KO.	Appl Environ Microbiol	10.1128/aem.59.9.2918-2926.1993	1993
	Pyrobaculum calidifontis sp. nov., a novel hyperthermophilic archaeon that grows in atmospheric air.	Amo T, Paje ML, Inagaki A, Ezaki S, Atomi H, Imanaka T.	Archaea	10.1155/2002/616075	2002
Phylogeny	Thermoproteus thermophilus sp. nov., a hyperthermophilic crenarchaeon isolated from solfataric soil.	June Yim K, Seon Song H, Choi JS, Woon Roh S	Int J Syst Evol Microbiol	10.1099/ijs.0.000293	2015

References

#713	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 2078
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Thermoproteus tenax (DSM 2078, ATCC 35583, JCM 9277)

Name and taxonomic classification

Morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Observation

Pathway annotation

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000253055

16S sequences

GC content

Genome-based predictions

Literature

Literature

References

BacDive

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