Name: Pyrobaculum neutrophilum V24Sta
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 17030

Type strain:

Species: Pyrobaculum neutrophilum

Strain Designation: V24Sta

Culture col. no.: DSM 2338, JCM 9278, NBRC 100436

Strain history: DSM 2338 <-- K. O. Stetter V24Sta.

NCBI tax ID(s): 444157 (strain), 70771 (species)

SI-ID 47586

DSM 2338, JCM 9278, NBRC 100436

Section

Pyrobaculum neutrophilum V24Sta is an anaerobe, hyperthermophilic archaeon that was isolated from hot spring.

anaerobe
hyperthermophilic
16S sequence
Archaea
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Archaea"
	Phylum	*Thermoproteota*
	Class	*Thermoprotei*
	Order	*Thermoproteales*
	Family	*Thermoproteaceae*
	Genus	*Pyrobaculum*
	Species	**Pyrobaculum neutrophilum**
	Full Scientific Name (LPSN)	Pyrobaculum neutrophilum (Stetter and Zillig 1989) Chan et al. 2013

Synonym

Thermoproteus neutrophilus

Information on culture and growth conditions Culture and growth conditions

[Ref.: #740]

Culture medium

PYROBACULUM NEUTROPHILUM MEDIUM (DSMZ Medium 291)

[Ref.: #740]

Culture medium growth

[Ref.: #740]

Culture medium link

Medium recipe at MediaDive

[Ref.: #740]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #740]			growth	85 °C
[Ref.: #67770]			growth	80 °C

[Ref.: #740]	Temperature range	hyperthermophilic
[Ref.: #67770]	Temperature range	hyperthermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #740]

Oxygen tolerance

anaerobe

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #740]	Sample type/isolated from	hot spring
[Ref.: #740]	Geographic location (country and/or sea, region)	Kerlingarfjoll
[Ref.: #740]	Country	Iceland
[Ref.: #740]	Country ISO 3 Code	ISL
[Ref.: #740]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Hot spring in Kerlingarjoll
[Ref.: #67770]	Country	Iceland
[Ref.: #67770]	Country ISO 3 Code	ISL
[Ref.: #67770]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Thermal spring
#Condition	#Thermophilic (>45°C)	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AB009618 (>99% sequence identity) for Pyrobaculum neutrophilum from Microbeatlas

Aquatic Samples	2
Soil Samples
Animal Samples
Plant Samples
Total Samples	2

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #740]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #740]	Thermoproteus neutrophilus gene for 16S rRNA, partial sequence	AB009618	2130	ENA	444157 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Pyrobaculum neutrophilum V24Sta	GCA_000019805	complete	GenBank	444157 tax ID	*
[Ref.: #66792]	Pyrobaculum neutrophilum V24Sta	641522657	complete	JGI IMG/M	444157 tax ID	*
[Ref.: #66792]	Pyrobaculum neutrophilum V24Sta	444157.8	complete	PATRIC	444157 tax ID	*

[Ref.: #740]

GC-content

56.0 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Pyrobaculum neutrophilum V24Sta V24Sta NCBI: GCA_000019805.1
[Ref.: #69480]	motile	no	73.271	no
[Ref.: #69480]	flagellated	no	94.875	no
[Ref.: #69480]	gram-positive	no	97.096	no
[Ref.: #69480]	anaerobic	yes	99.37	yes
[Ref.: #69480]	aerobic	no	96.939	yes
[Ref.: #69480]	halophile	no	86.783	no
[Ref.: #69480]	spore-forming	no	96.606	no
[Ref.: #69480]	thermophile	yes	100	no
[Ref.: #69480]	glucose-util	yes	75.308	no
[Ref.: #69480]	glucose-ferment	no	70.056	no

Availability in culture collections External links

[Ref.: #740]

Culture collection no.

DSM 2338, JCM 9278, NBRC 100436

[Ref.: #86031]

SI-ID 47586

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Reclassification of Thermoproteus neutrophilus Stetter and Zillig 1989 as Pyrobaculum neutrophilum comb. nov. based on phylogenetic analysis.	Chan PP, Cozen AE, Lowe TM	Int J Syst Evol Microbiol	10.1099/ijs.0.043091-0	2012	*
Phylogeny	Occurrence of introns in the 16S rRNA genes of members of the genus Thermoproteus.	Itoh T, Suzuki K, Nakase T	Arch Microbiol	10.1007/s002030050628	1998	*

References

#740	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 2338

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#86031

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID47586.1 )

* These data were automatically processed and therefore are not curated

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Pyrobaculum neutrophilum V24Sta V24Sta

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