Archive BacDiveID:10.13145/bacdive17031.20230509.8.1

Strain identifier

BacDive ID: 17031

Type strain:

Species: Thermoproteus tenax

Strain history: DSM 2078 <-- W. Zillig Kra1.

NCBI tax ID(s): 768679 (strain), 2271 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.

General

@ref: 713

BacDive-ID: 17031

DSM-Number: 2078

keywords: genome sequence, 16S sequence, Archaea, anaerobe, hyperthermophilic

description: Thermoproteus tenax DSM 2078 is an anaerobe, hyperthermophilic archaeon that was isolated from mud hole, solfataric field.

NCBI tax id

NCBI tax id	Matching level
2271	species
768679	strain

strain history

@ref	history
713	<- W. Zillig, Kra 1
67770	DSM 2078 <-- W. Zillig Kra1.

doi: 10.13145/bacdive17031.20230509.8.1

Name and taxonomic classification

LPSN

@ref: 20215
description: domain/archaea
keyword: phylum/thermoproteota
domain: Archaea
phylum: Thermoproteota
class: Thermoprotei
order: Thermoproteales
family: Thermoproteaceae
genus: Thermoproteus
species: Thermoproteus tenax
full scientific name: Thermoproteus tenax Zillig and Stetter 1982

@ref: 713

domain: Archaea

phylum: Crenarchaeota

class: Thermoprotei

order: Thermoproteales

family: Thermoproteaceae

genus: Thermoproteus

species: Thermoproteus tenax

full scientific name: Thermoproteus tenax Zillig and Stetter 1982

type strain: yes

Culture and growth conditions

culture medium

@ref: 713
name: THERMOPROTEUS MEDIUM (DSMZ Medium 185)
growth: yes
link: https://mediadive.dsmz.de/medium/185
composition: Name: THERMOPROTEUS MEDIUM (DSMZ Medium 185) Composition: Sulfur 10.0 g/l Starch 5.0 g/l FeSO4 x 7 H2O 0.556 g/l Na2S x 9 H2O 0.5 g/l MgSO4 x 7 H2O 0.492 g/l CaSO4 x 2 H2O 0.344 g/l (NH4)2SO4 0.264 g/l Yeast extract 0.2 g/l KH2PO4 0.014 g/l Resazurin 0.001 g/l NaF 0.00084 g/l Na2B4O7 x 10 H2O 0.00045 g/l MnCl2 x 4 H2O 0.00018 g/l ZnSO4 x 7 H2O 2.2e-05 g/l CuCl2 x 2 H2O 5e-06 g/l Na2MoO4 x 2 H2O 3e-06 g/l CoSO4 x 7 H2O 1e-06 g/l Distilled water

culture temp

@ref	growth	type	temperature	range
713	positive	growth	85	hyperthermophilic
67770	positive	growth	85	hyperthermophilic

Physiology and metabolism

oxygen tolerance

@ref: 713
oxygen tolerance: anaerobe

observation

@ref: 67770
observation: quinones: MK-6(H₁₂), MK-5(H₁₀), MK-4(H₈), MK-6(H₁₀), MK-5(H₈), MK-4(H₆), MMK-6(H₁₂), MMK-5(H₁₀)

Isolation, sampling and environmental information

isolation

@ref	sample type	geographic location	country	origin.country	continent
713	mud hole, solfataric field	Krafta	Iceland	ISL	Europe
67770	Acidic solfataric hot spring	Krafla	Iceland	ISL	Europe

isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Mud (Sludge)
#Environmental	#Terrestrial	#Volcanic

Safety information

risk assessment

@ref: 713
biosafety level: 1
biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@ref: 713
description: T.tenax 16S rRNA
accession: M35966.1
length: 1504
database: ena
NCBI tax ID: 2271

Genome sequences

@ref: 67770
description: Thermoproteus tenax Kra 1
accession: GCA_000253055
assembly level: complete
database: ncbi
NCBI tax ID: 768679

GC content

@ref	GC-content	method
713	55.5
67770	55.9	thermal denaturation, midpoint method (Tm)
67770	55.3	high performance liquid chromatography (HPLC)

External links

@ref: 713

culture collection no.: DSM 2078, ATCC 35583, JCM 9277, Kra 1, NBRC 100435

straininfo link

@ref: 86032
straininfo: 41172

literature

topic	Pubmed-ID	title	authors	journal	DOI	year	mesh	topic2
Enzymology	2505050	Identification and characterization of the genes encoding three structural proteins of the Thermoproteus tenax virus TTV1.	Neumann H, Schwass V, Eckerskorn C, Zillig W	Mol Gen Genet	10.1007/BF00330948	1989	Amino Acid Sequence, Archaea/genetics, Bacteria/genetics, Bacteriophages/genetics, Base Sequence, Binding Sites, Blotting, Northern, Codon, DNA, Viral/isolation & purification, Electrophoresis, Polyacrylamide Gel, Genes, Genes, Viral, Molecular Sequence Data, Promoter Regions, Genetic, RNA, Messenger/genetics, RNA, Ribosomal, 16S/metabolism, RNA, Viral/genetics, Restriction Mapping, Viral Proteins/genetics, Viral Structural Proteins	Phylogeny
Genetics	22003381	The complete genome sequence of Thermoproteus tenax: a physiologically versatile member of the Crenarchaeota.	Siebers B, Zaparty M, Raddatz G, Tjaden B, Albers SV, Bell SD, Blombach F, Kletzin A, Kyrpides N, Lanz C, Plagens A, Rampp M, Rosinus A, von Jan M, Makarova KS, Klenk HP, Schuster SC, Hensel R	PLoS One	10.1371/journal.pone.0024222	2011	Amino Acids/biosynthesis, Chemoautotrophic Growth/genetics, DNA Replication/genetics, Energy Metabolism/genetics, Evolution, Molecular, Genome, Archaeal/genetics, Genomics, Phylogeny, Protein Biosynthesis/genetics, Protein Transport/genetics, Proton-Motive Force/genetics, Thermoproteus/genetics/metabolism/*physiology, Transcription, Genetic/genetics	Metabolism
Phylogeny	25933622	Thermoproteus thermophilus sp. nov., a hyperthermophilic crenarchaeon isolated from solfataric soil.	June Yim K, Seon Song H, Choi JS, Woon Roh S	Int J Syst Evol Microbiol	10.1099/ijs.0.000293	2015	Base Composition, DNA, Archaeal/genetics, Molecular Sequence Data, Nucleic Acid Hybridization, Philippines, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Soil Microbiology, Thermoproteus/*classification/genetics/isolation & purification	Genetics

Reference

@id	authors	catalogue	doi/url	title	journal	pubmed
713	Curators of the DSMZ	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 2078)	https://www.dsmz.de/collection/catalogue/details/culture/DSM-2078
20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.		10.1099/ijsem.0.004332	List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg		10.1093/nar/gkaa1025	BRENDA, the ELIXIR core data resource in 2021: new developments and updates	Nucleic Acids Res. 49: D498-D508 2020	33211880
67770	Curators of the JCM		https://jcm.brc.riken.jp/en/
86032	Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.		10.60712/SI-ID41172.1	StrainInfo: A central database for resolving microbial strain identifiers