Thermococcus sibiricus (DSM 12597, MM 739, B-2774)

Name: Thermococcus sibiricus (DSM 12597, MM 739, B-2774)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 16880

Type strain

Strain Designation MM 739, B-2774

Culture col. no. DSM 12597 MM 739 B-2774

NCBI tax ID(s) 172049

Special Collections DSMZ

History DSMZ <- E. A. Bonch-Osmolovskaya, Russian Academy of Sciences, Inst. of Microbiology, Moscow; MM 739

Links

Thermococcus sibiricus MM 739 is an anaerobe archaeon that was isolated from formation water, high temperature Samoltor oil reservoir.

anaerobe genome sequence 16S sequence Archaea

Name and taxonomic classification

SI-ID 49330

VKM:B-2774,DSM 12597

@ref 20215

Last LPSN update 2026-05-29 08:28:53

Domain Archaea

Phylum Methanobacteriota

Class Thermococci

Order Thermococcales

Family Thermococcaceae

Genus Thermococcus

Species Thermococcus sibiricus

Full scientific name Thermococcus sibiricus Miroshnichenko et al. 2024

Morphology

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
4769	MARINE THERMOCOCCUS MEDIUM (DSMZ Medium 760)		Medium recipe at MediaDive	Name: MARINE THERMOCOCCUS MEDIUM (DSMZ Medium 760) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
4769	positive	growth	78

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
4769	anaerobe
125438	anaerobe	90.369
125439	anaerobe	98.143

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Industrial	#Oil reservoir
#Environmental	#Aquatic	-
#Condition	#Thermophilic (>45°C)	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
4769	formation water, high temperature Samoltor oil reservoir	Western Siberia	Russia	RUS	Asia

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
4769	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM2254v1 assembly for Thermococcus sibiricus MM 739	complete	GCA_000022545	604354.9	644736412	604354	99.12

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
4769	Thermococcus sibiricus partial 16S rRNA gene for 16S ribosomal RNA	AJ238992	1305		604354

GC content

4769

GC-content (mol%)38.4

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM2254v1 assembly for Thermococcus sibiricus MM 739
BV-BRC: 604354.9

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	anaerobe	98.14	no
125439	motility	BacteriaNetⓘ	no	70.03	no
125439	gram_stain	BacteriaNetⓘ	negative	44.46	no
125439	spore_formation	BacteriaNetⓘ	no	82.83	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Thermococcus sibiricus MM 739 MM 739
NCBI: GCA_000022545.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	66.76	no
125438	anaerobic	anaerobicⓘ	yes	90.37	yes
125438	aerobic	aerobicⓘ	no	87.70	yes
125438	spore-forming	spore-formingⓘ	no	89.27	no
125438	thermophilic	thermophileⓘ	yes	81.05	yes
125438	flagellated	motile2+ⓘ	no	79.58	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Validation List no. 219. Valid publication of new names and new combinations effectively published outside the IJSEM.	Oren A, Goker M.	Int J Syst Evol Microbiol	10.1099/ijsem.0.006452	2024
Metabolism	Conditional Protein Splicing Switch in Hyperthermophiles through an Intein-Extein Partnership.	Lennon CW, Stanger M, Banavali NK, Belfort M.	mBio	10.1128/mbio.02304-17	2018
Metabolism	Metabolic versatility and indigenous origin of the archaeon Thermococcus sibiricus, isolated from a siberian oil reservoir, as revealed by genome analysis.	Mardanov AV, Ravin NV, Svetlitchnyi VA, Beletsky AV, Miroshnichenko ML, Bonch-Osmolovskaya EA, Skryabin KG.	Appl Environ Microbiol	10.1128/aem.00718-09	2009
Phylogeny	Isolation and characterization of Thermococcus sibiricus sp. nov. from a Western Siberia high-temperature oil reservoir.	Miroshnichenko ML, Hippe H, Stackebrandt E, Kostrikina NA, Chernyh NA, Jeanthon C, Nazina TN, Belyaev SS, Bonch-Osmolovskaya EA	Extremophiles	10.1007/s007920100175	2001

References

#4769	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 12597
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Thermococcus sibiricus (DSM 12597, MM 739, B-2774)

Name and taxonomic classification

Morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000022545

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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