Strain identifier

BacDive ID: 16880

Type strain: Yes

Species: Thermococcus sibiricus

Strain Designation: MM 739, B-2774

Strain history: <- E. A. Bonch-Osmolovskaya, Russian Academy of Sciences, Inst. of Microbiology, Moscow; MM 739

NCBI tax ID(s): 172049 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 4769

BacDive-ID: 16880

DSM-Number: 12597

keywords: genome sequence, 16S sequence, Archaea, anaerobe, thermophilic

description: Thermococcus sibiricus MM 739 is an anaerobe, thermophilic archaeon that was isolated from formation water, high temperature Samoltor oil reservoir.

NCBI tax id

  • NCBI tax id: 172049
  • Matching level: species

strain history

  • @ref: 4769
  • history: <- E. A. Bonch-Osmolovskaya, Russian Academy of Sciences, Inst. of Microbiology, Moscow; MM 739

doi: 10.13145/bacdive16880.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/archaea
  • keyword: phylum/methanobacteriota
  • domain: Archaea
  • phylum: Methanobacteriota
  • class: Thermococci
  • order: Thermococcales
  • family: Thermococcaceae
  • genus: Thermococcus
  • species: Thermococcus sibiricus
  • full scientific name: Thermococcus sibiricus Miroshnichenko et al. 2001

@ref: 4769

domain: Archaea

phylum: Euryarchaeota

class: Thermococci

order: Thermococcales

family: Thermococcaceae

genus: Thermococcus

species: Thermococcus sibiricus

full scientific name: Thermococcus sibiricus Miroshnichenko et al. 2001

strain designation: MM 739, B-2774

type strain: yes

Culture and growth conditions

culture medium

  • @ref: 4769
  • name: MARINE THERMOCOCCUS MEDIUM (DSMZ Medium 760)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/760
  • composition: Name: MARINE THERMOCOCCUS MEDIUM (DSMZ Medium 760) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water

culture temp

  • @ref: 4769
  • growth: positive
  • type: growth
  • temperature: 78
  • range: thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 4769
  • oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

  • @ref: 4769
  • sample type: formation water, high temperature Samoltor oil reservoir
  • geographic location: Western Siberia
  • country: Russia
  • origin.country: RUS
  • continent: Asia

isolation source categories

Cat1Cat2Cat3
#Engineered#Industrial#Oil reservoir
#Environmental#Aquatic
#Condition#Thermophilic (>45°C)

Safety information

risk assessment

  • @ref: 4769
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 4769
  • description: Thermococcus sibiricus partial 16S rRNA gene for 16S ribosomal RNA
  • accession: AJ238992
  • length: 1305
  • database: ena
  • NCBI tax ID: 604354

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Thermococcus sibiricus MM 739GCA_000022545completencbi604354
66792Thermococcus sibiricus MM 739644736412completeimg604354
66792Thermococcus sibiricus MM 739604354.9completepatric604354

GC content

  • @ref: 4769
  • GC-content: 38.4

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno77.026no
flagellatedno97.072no
gram-positiveno96.065no
anaerobicyes98.28yes
aerobicno97.365yes
halophileno67.905no
spore-formingno97.491no
glucose-utilyes80.814no
thermophileyes99.999yes
glucose-fermentno61.514no

External links

@ref: 4769

culture collection no.: DSM 12597

straininfo link

  • @ref: 85888
  • straininfo: 49330

literature

  • topic: Phylogeny
  • Pubmed-ID: 11354459
  • title: Isolation and characterization of Thermococcus sibiricus sp. nov. from a Western Siberia high-temperature oil reservoir.
  • authors: Miroshnichenko ML, Hippe H, Stackebrandt E, Kostrikina NA, Chernyh NA, Jeanthon C, Nazina TN, Belyaev SS, Bonch-Osmolovskaya EA
  • journal: Extremophiles
  • DOI: 10.1007/s007920100175
  • year: 2001
  • mesh: DNA, Archaeal/genetics, DNA, Ribosomal/genetics, *Hot Temperature, Nucleic Acid Hybridization, *Petroleum, Phylogeny, RNA, Ribosomal, 16S/genetics, Siberia, Thermococcus/classification/genetics/*isolation & purification
  • topic2: Stress

Reference

@idauthorscataloguedoi/urltitle
4769Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 12597)https://www.dsmz.de/collection/catalogue/details/culture/DSM-12597
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
85888Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID49330.1StrainInfo: A central database for resolving microbial strain identifiers