Streptomyces spiralis (DSM 43836, AS 4.1852, ATCC 25664)

Cell morphology

Colony morphology

Multicellular morphology

Multimedia

• @ref:	11320
  multimedia content:	DSM_43836-1.jpg
  multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_43836-1.jpg
  intellectual property rights:	© Leibniz-Institut DSMZ
  manual_annotation:	1

• @ref:	11320
  multimedia content:	DSM_43836.jpg
  multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_43836.jpg
  caption:	Medium 65 28°C
  intellectual property rights:	© Leibniz-Institut DSMZ
  manual_annotation:	1

Culture medium

@ref	Name	Growth	Medium link	Composition
11320	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)		Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
11320	GPHF-MEDIUM (DSMZ Medium 553)		Medium recipe at MediaDive	Name: GPHF-MEDIUM (DSMZ Medium 553) Composition: Agar 20.0 g/l Glucose 10.0 g/l Beef extract 5.0 g/l Yeast extract 5.0 g/l Casein peptone 5.0 g/l CaCl2 x 2 H2O 0.74 g/l Distilled water
19963	ISP 2			Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19963	ISP 3			Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19963	ISP 4			Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19963	ISP 5			Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19963	ISP 6			Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19963	ISP 7			Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes

Culture temp

Oxygen tolerance

Spore formation

Halophily

Observation

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
19963	22599 ChEBI	arabinose	+/-
68368	29016 ChEBI	arginine	+	hydrolysis	from API 20E
19963	62968 ChEBI	cellulose	-
68368	16947 ChEBI	citrate	+	assimilation	from API 20E
19963	28757 ChEBI	fructose	+
68368	5291 ChEBI	gelatin	+	hydrolysis	from API 20E
19963	17234 ChEBI	glucose	+
68368	25094 ChEBI	lysine	-	degradation	from API 20E
19963	29864 ChEBI	mannitol	+/-
19963	17268 ChEBI	myo-inositol	-
68368	18257 ChEBI	ornithine	-	degradation	from API 20E
19963	16634 ChEBI	raffinose	-
19963	26546 ChEBI	rhamnose	+
19963	17992 ChEBI	sucrose	+
68368	27897 ChEBI	tryptophan	+	energy source	from API 20E
68368	16199 ChEBI	urea	+	hydrolysis	from API 20E
19963	18222 ChEBI	xylose	-

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68368	15688 ChEBI	acetoin		from API 20E
68368	16136 ChEBI	hydrogen sulfide		from API 20E
68368	35581 ChEBI	indole		from API 20E

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Indole test
68368	15688 ChEBI	acetoin	-		from API 20E
68368	35581 ChEBI	indole		+	from API 20E

Enzymes

@ref	Value	Activity	Ec
68368	arginine dihydrolase	+	3.5.3.6	from API 20E
68368	beta-galactosidase	+	3.2.1.23	from API 20E
68368	gelatinase	+		from API 20E
68368	lysine decarboxylase	-	4.1.1.18	from API 20E
68368	ornithine decarboxylase	-	4.1.1.17	from API 20E
68368	tryptophan deaminase	+	4.1.99.1	from API 20E
68368	urease	+	3.5.1.5	from API 20E

API 20E

Isolation source categories

Isolation

Risk assessment

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
124043	ASM4242862v1 assembly for Streptomyces spiralis JCM 3302	scaffold	GCA_042428625			66376	39.65
66792	ASM1465467v1 assembly for Streptomyces spiralis JCM 3302	scaffold	GCA_014654675	66376.3	8109890078	66376	22.59

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	Streptomyces spiralis gene for 16S rRNA, partial sequence	AB122727	565		66376
20218	Streptomyces spiralis gene for 16S ribosomal RNA, partial sequence, strain: JCM 3302	D43983	121		66376
20218	Streptomyces spiralis gene for 16S rRNA, partial sequence, strain: NBRC 14215	AB184575	1463		66376
20218	Streptomyces spiralis strain NRRL B-16922 16S ribosomal RNA gene, partial sequence	EF178683	1446		66376
124043	Streptomyces spiralis strain JCM 3302 16S ribosomal RNA gene, partial sequence.	MT760440	1378		66376

Predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
	Streptomyces sp. JCK-6131 Protects Plants Against Bacterial and Fungal Diseases via Two Mechanisms.	Le KD, Kim J, Nguyen HT, Yu NH, Park AR, Lee CW, Kim JC.	Front Plant Sci	10.3389/fpls.2021.726266	2021
	Actinomycete strain type determines the monodispersity and antibacterial properties of biogenically synthesized silver nanoparticles.	Mabrouk M, Elkhooly TA, Amer SK.	J Genet Eng Biotechnol	10.1186/s43141-021-00153-y	2021
	Performance of three endophytic actinomycetes in relation to plant growth promotion and biological control of Pythium aphanidermatum, a pathogen of cucumber under commercial field production conditions in the United Arab Emirates	El-Tarabily KA, St. J. Hardy GE, Sivasithamparam K.	Eur J Plant Pathol	10.1007/s10658-010-9689-7	2010
Metabolism	Biosynthesis and Characterization of Extracellular Silver Nanoparticles from Streptomyces aizuneusis: Antimicrobial, Anti Larval, and Anticancer Activities.	Abd-Elhady HM, Ashor MA, Hazem A, Saleh FM, Selim S, El Nahhas N, Abdel-Hafez SH, Sayed S, Hassan EA.	Molecules	10.3390/molecules27010212	2021
	Plant growth promotion and biological control of Pythium aphanidermatum, a pathogen of cucumber, by endophytic actinomycetes.	El-Tarabily KA, Nassar AH, Hardy GE, Sivasithamparam K.	J Appl Microbiol	10.1111/j.1365-2672.2008.03926.x	2009
	Anti-inflammatory effect and antihepatoma mechanism of carrimycin.	Li XY, Luo YT, Wang YH, Yang ZX, Shang YZ, Guan QX.	World J Gastroenterol	10.3748/wjg.v29.i14.2134	2023
	Plant Growth Promoting and Biocontrol Activity of Streptomyces spp. as Endophytes.	Vurukonda SSKP, Giovanardi D, Stefani E.	Int J Mol Sci	10.3390/ijms19040952	2018
	Biocontrol Potential of Endophytic Actinobacteria against Fusarium solani, the Causal Agent of Sudden Decline Syndrome on Date Palm in the UAE.	Alblooshi AA, Purayil GP, Saeed EE, Ramadan GA, Tariq S, Altaee AS, El-Tarabily KA, AbuQamar SF.	J Fungi (Basel)	10.3390/jof8010008	2021
Phylogeny	New antitumor antibiotic, FR-900462. I. Taxonomy of the producing strain.	Iwami M, Nakayama O, Terano H, Kohsaka M.	J Antibiot (Tokyo)	10.7164/antibiotics.42.680	1989
	Biological Control of Mango Dieback Disease Caused by Lasiodiplodia theobromae Using Streptomycete and Non-streptomycete Actinobacteria in the United Arab Emirates.	Kamil FH, Saeed EE, El-Tarabily KA, AbuQamar SF.	Front Microbiol	10.3389/fmicb.2018.00829	2018
Phylogeny	Streptomyces mahasarakhamensis sp. nov., an Endophytic Actinobacterium Isolated from Jasmine Rice and its Potential as plant Growth Promoter.	Sukpanoa S, Kaewkla O, Suriyachadkun C, Papayrata C, Klankeo P, Franco CMM.	Curr Microbiol	10.1007/s00284-024-03747-0	2024
Phylogeny	Streptomyces sennicomposti sp. nov., an actinomycete isolated from compost of Senna siamea (Lam.).	Duangupama T, Pittayakhajonwut P, Intaraudom C, Suriyachadkun C, Sirirote P, He YW, Thawai C	Int J Syst Evol Microbiol	10.1099/ijsem.0.005320	2022