Strain identifier

BacDive ID: 16211

Type strain: Yes

Species: Streptomyces spiralis

Strain Designation: C9A-IBc, Mc 9H

Strain history: KCC A-0302 <-- S. T. Williams MC 9H <-- J. O. Falcão de Morais C9A-IBc.

NCBI tax ID(s): 66376 (species)

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General

@ref: 11320

BacDive-ID: 16211

DSM-Number: 43836

keywords: genome sequence, 16S sequence, Bacteria, spore-forming

description: Streptomyces spiralis C9A-IBc is a spore-forming bacterium that builds an aerial mycelium and was isolated from soil.

NCBI tax id

  • NCBI tax id: 66376
  • Matching level: species

strain history

@refhistory
11320<- KCC <- S.T. Williams, Mc 9H <- J.O. Falcao de Morias, C9A-IBc (Elytrosporangium spirale)
67770KCC A-0302 <-- S. T. Williams MC 9H <-- J. O. Falcão de Morais C9A-IBc.

doi: 10.13145/bacdive16211.20240916.9.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces spiralis
  • full scientific name: Streptomyces spiralis (Falcão de Morais 1970) Goodfellow et al. 1986

  • synonyms

    • @ref: 20215
    • synonym: Elytrosporangium spirale

@ref: 11320

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces spiralis

full scientific name: Streptomyces spiralis (Falcão de Morais 1970) Goodfellow et al. 1986

strain designation: C9A-IBc, Mc 9H

type strain: yes

Morphology

colony morphology

@refcolony colorincubation periodmedium used
19963Beige10-14 daysISP 2
19963Brown10-14 daysISP 3
19963Brown10-14 daysISP 4
19963Beige10-14 daysISP 5
19963Beige10-14 daysISP 6
19963Beige10-14 daysISP 7

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
19963yesAerial MyceliumWhiteISP 2
19963yesAerial MyceliumGreyISP 3
19963yesAerial MyceliumWhiteISP 4
19963yesAerial MyceliumWhiteISP 5
19963yesAerial MyceliumWhiteISP 6
19963yesAerial MyceliumWhiteISP 7

multimedia

@refmultimedia contentintellectual property rightscaption
11320https://www.dsmz.de/microorganisms/photos/DSM_43836-1.jpg© Leibniz-Institut DSMZ
11320https://www.dsmz.de/microorganisms/photos/DSM_43836.jpg© Leibniz-Institut DSMZMedium 65 28°C

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
11320GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
11320GPHF-MEDIUM (DSMZ Medium 553)yeshttps://mediadive.dsmz.de/medium/553Name: GPHF-MEDIUM (DSMZ Medium 553) Composition: Agar 20.0 g/l Glucose 10.0 g/l Beef extract 5.0 g/l Yeast extract 5.0 g/l Casein peptone 5.0 g/l CaCl2 x 2 H2O 0.74 g/l Distilled water
19963ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19963ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19963ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19963ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19963ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19963ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes

culture temp

@refgrowthtypetemperature
11320positivegrowth28
19963positiveoptimum28
67770positivegrowth28

Physiology and metabolism

oxygen tolerance

  • @ref: 69480
  • oxygen tolerance: aerobe
  • confidence: 91.028

spore formation

@refspore descriptionspore formationconfidence
19963Formation of spore chains (rectiflixibilis), spore surface smooth, fragmentationyes
69480yes91.697
69481yes100

halophily

  • @ref: 19963
  • salt: NaCl
  • growth: positive
  • tested relation: maximum
  • concentration: 5 %

observation

  • @ref: 67770
  • observation: quinones: MK-9(H8), MK-9(H6), MK-9(H4), MK-9(H2)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1996362968cellulose-
1996316634raffinose-
1996326546rhamnose+
1996328757fructose+
1996329864mannitol+/-
1996317268myo-inositol-
1996318222xylose-
1996317992sucrose+
1996322599arabinose+/-
1996317234glucose+
683685291gelatin+hydrolysis
6836827897tryptophan+energy source
6836816199urea+hydrolysis
6836816947citrate+assimilation
6836818257ornithine-degradation
6836825094lysine-degradation
6836829016arginine+hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinno
6836835581indoleyes
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin-
6836835581indole+

enzymes

@refvalueactivityec
68368gelatinase+
68368tryptophan deaminase+4.1.99.1
68368urease+3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase+3.5.3.6
68368beta-galactosidase+3.2.1.23

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
19963++--+-+++-+

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
11320soil
67770SoilRecifeBrazilBRAMiddle and South America

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
113201Risk group (German classification)
199631German classification

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptomyces spiralis gene for 16S rRNA, partial sequenceAB122727565nuccore66376
20218Streptomyces spiralis gene for 16S ribosomal RNA, partial sequence, strain: JCM 3302D43983121nuccore66376
20218Streptomyces spiralis gene for 16S rRNA, partial sequence, strain: NBRC 14215AB1845751463nuccore66376
20218Streptomyces spiralis strain NRRL B-16922 16S ribosomal RNA gene, partial sequenceEF1786831446nuccore66376

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Streptomyces spiralis JCM 3302GCA_014654675scaffoldncbi66376
66792Streptomyces spiralis strain JCM 330266376.3wgspatric66376

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69481spore-formingspore-formingAbility to form endo- or exosporesyes100no
69480gram-positivegram-positivePositive reaction to Gram-stainingyes87.308no
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no96.066no
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)yes91.028no
69480spore-formingspore-formingAbility to form endo- or exosporesyes91.697yes
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cno97.5yes
69480flagellatedmotile2+Ability to perform flagellated movementno88no

External links

@ref: 11320

culture collection no.: DSM 43836, AS 4.1852, ATCC 25664, IFO 14215, JCM 3302, KCC 3302, KCC A-0302, NBRC 14215, CGMCC 4.1764, CGMCC 4.1852, KCTC 9849, MTCC 501, NRRL B-16922, VKM Ac-1311

straininfo link

  • @ref: 85272
  • straininfo: 44726

literature

  • topic: Phylogeny
  • Pubmed-ID: 35446756
  • title: Streptomyces sennicomposti sp. nov., an actinomycete isolated from compost of Senna siamea (Lam.).
  • authors: Duangupama T, Pittayakhajonwut P, Intaraudom C, Suriyachadkun C, Sirirote P, He YW, Thawai C
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.005320
  • year: 2022
  • mesh: *Actinobacteria/genetics, Bacterial Typing Techniques, Base Composition, *Composting, DNA, Bacterial/genetics, Fatty Acids/chemistry, Nucleic Acid Hybridization, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Streptomyces, Thailand
  • topic2: Transcriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
11320Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 43836)https://www.dsmz.de/collection/catalogue/details/culture/DSM-43836
19963Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM43836.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
85272Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID44726.1StrainInfo: A central database for resolving microbial strain identifiers