Name: Pseudoruegeria insulae BPTF-M20
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 159803

Type strain:

Species: Pseudoruegeria insulae

Strain Designation: BPTF-M20

Culture col. no.: KACC 19614, KCTC 62422, NBRC 113188

Strain history: <- Jung-Hoon YOON, Sungkyunkwan Univ.

NCBI tax ID(s): 2231601 (species)

Section

Pseudoruegeria insulae BPTF-M20 is an aerobe, psychrophilic, Gram-negative bacterium that forms circular colonies and was isolated from a tidal flat sediment.

colony-forming
Gram-negative
rod-shaped
aerobe
psychrophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Rhodobacterales*
	Family	*Roseobacteraceae*
	Genus	*Pseudoruegeria*
	Species	**Pseudoruegeria insulae**
	Full Scientific Name (LPSN)	Pseudoruegeria insulae Park et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #66745]	Gram stain	negative

[Ref.: #66745]	Cell length	0.4-10.0 µm

[Ref.: #66745]	Cell width	0.4-0.8 µm

[Ref.: #66745]	Cell shape	rod-shaped

[Ref.: #66745]	Colony size	0.5-1 mm
[Ref.: #66745]	Colony color	greyish yellow
[Ref.: #66745]	Colony shape	circular
[Ref.: #66745]	Incubation period	5 days
[Ref.: #66745]	Cultivation medium used	Marine agar (MA)

Information on culture and growth conditions Culture and growth conditions

[Ref.: #66745]

Culture medium

Marine agar (MA)

[Ref.: #66745]

Culture medium growth

	Kind of temperature		Temperature
[Ref.: #66745]	no	growth	4 °C
[Ref.: #66745]		optimum	30 °C
[Ref.: #66745]	no	growth	37 °C
[Ref.: #66745]		growth	10-35 °C
[Ref.: #67771]		growth	30 °C

[Ref.: #66745]	Temperature range	psychrophilic
[Ref.: #66745]	Temperature range	mesophilic
[Ref.: #67771]	Temperature range	mesophilic

	Kind of pH		pH
[Ref.: #66745]		optimum	7-8
[Ref.: #66745]	no	growth	5.5
[Ref.: #66745]		growth	6

Information on physiology and metabolism Physiology and metabolism

[Ref.: #67771]

Oxygen tolerance

aerobe

[Ref.: #66745]

Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #66745]		NaCl		growth	1.5-5.0	%(w/v)
[Ref.: #66745]		NaCl		optimum	2.0-3.0	%(w/v)

[Ref.: #66745]	Observation	Mg2+ ions are required for growth.
[Ref.: #66745]	Observation	The predominant ubiquinone is Q-10.
[Ref.: #66745]	Observation	The major polar lipids are phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, one unidentified aminolipid and one unidentified glycolipid.

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #66745]	benzoate ChEBI	-	carbon source
[Ref.: #66745]	benzoate ChEBI	-	energy source
[Ref.: #66745]	casein	-	hydrolysis
[Ref.: #66745]	cellobiose ChEBI	-	carbon source
[Ref.: #66745]	cellobiose ChEBI	-	energy source
[Ref.: #66745]	D-fructose ChEBI	-	carbon source
[Ref.: #66745]	D-fructose ChEBI	-	energy source
[Ref.: #66745]	D-galactose ChEBI	-	carbon source
[Ref.: #66745]	D-galactose ChEBI	-	energy source
[Ref.: #66745]	D-glucose ChEBI	-	carbon source
[Ref.: #66745]	D-glucose ChEBI	-	energy source
[Ref.: #66745]	D-mannose ChEBI	-	carbon source
[Ref.: #66745]	D-mannose ChEBI	-	energy source
[Ref.: #66745]	D-xylose ChEBI	-	carbon source
[Ref.: #66745]	D-xylose ChEBI	-	energy source
[Ref.: #66745]	esculin ChEBI	-	hydrolysis
[Ref.: #66745]	gelatin ChEBI	-	hydrolysis
[Ref.: #66745]	hypoxanthine ChEBI	-	hydrolysis
[Ref.: #66745]	L-arabinose ChEBI	-	carbon source
[Ref.: #66745]	L-arabinose ChEBI	-	energy source
[Ref.: #66745]	L-glutamate ChEBI	-	carbon source
[Ref.: #66745]	L-glutamate ChEBI	-	energy source
[Ref.: #66745]	L-malate ChEBI	-	carbon source
[Ref.: #66745]	L-malate ChEBI	-	energy source
[Ref.: #66745]	L-tyrosine ChEBI	-	hydrolysis
[Ref.: #66745]	maltose ChEBI	-	carbon source
[Ref.: #66745]	maltose ChEBI	-	energy source
[Ref.: #66745]	salicin ChEBI	-	carbon source
[Ref.: #66745]	salicin ChEBI	-	energy source
[Ref.: #66745]	sodium acetate ChEBI	-	carbon source
[Ref.: #66745]	sodium acetate ChEBI	-	energy source
[Ref.: #66745]	sodium citrate ChEBI	-	carbon source
[Ref.: #66745]	sodium citrate ChEBI	-	energy source
[Ref.: #66745]	sodium formate ChEBI	-	carbon source
[Ref.: #66745]	sodium formate ChEBI	-	energy source
[Ref.: #66745]	starch ChEBI	-	hydrolysis
[Ref.: #66745]	sucrose ChEBI	-	carbon source
[Ref.: #66745]	sucrose ChEBI	-	energy source
[Ref.: #66745]	trehalose ChEBI	-	carbon source
[Ref.: #66745]	trehalose ChEBI	-	energy source
[Ref.: #66745]	tween 80 ChEBI	-	hydrolysis
[Ref.: #66745]	urea ChEBI	-	hydrolysis
[Ref.: #66745]	xanthine ChEBI	-	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #66745]	acid phosphatase	-	3.1.3.2
[Ref.: #66745]	alkaline phosphatase	+	3.1.3.1
[Ref.: #66745]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #66745]	alpha-fucosidase	-	3.2.1.51
[Ref.: #66745]	alpha-galactosidase	-	3.2.1.22
[Ref.: #66745]	alpha-glucosidase	+	3.2.1.20
[Ref.: #66745]	alpha-mannosidase	-	3.2.1.24
[Ref.: #66745]	beta-galactosidase	-	3.2.1.23
[Ref.: #66745]	beta-glucosidase	-	3.2.1.21
[Ref.: #66745]	beta-glucuronidase	-	3.2.1.31
[Ref.: #66745]	catalase	+	1.11.1.6
[Ref.: #66745]	cystine arylamidase	-	3.4.11.3
[Ref.: #66745]	cytochrome oxidase	+	1.9.3.1
[Ref.: #66745]	esterase (C 4)	+
[Ref.: #66745]	esterase Lipase (C 8)	+
[Ref.: #66745]	leucine arylamidase	+	3.4.11.1
[Ref.: #66745]	lipase (C 14)	-
[Ref.: #66745]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #66745]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #66745]	nitrate reductase	+	1.7.99.4
[Ref.: #66745]	trypsin	-	3.4.21.4
[Ref.: #66745]	valine arylamidase	-
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #66745]

Fatty acid	Percentage
C18:1 w7c	90.7
C16:0	4.6
11-methyl C18:1 w7c	1.2
C18:0	1.2
C20:1 w7c	0.7

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #66745]	Sample type/isolated from	a tidal flat sediment
[Ref.: #66745]	Geographic location (country and/or sea, region)	Anmyeon island on the Yellow Sea
[Ref.: #66745]	Country	Republic of Korea
[Ref.: #66745]	Country ISO 3 Code	KOR
[Ref.: #66745]	Continent	Asia
[Ref.: #66745]	Enrichment culture	marine agar 2216 (MA; BD Difco).
[Ref.: #66745]	Enrichment culture duration	1 week
[Ref.: #66745]	Enrichment culture temperature	25 ̊C
[Ref.: #66745]	Isolation/enrichment procedure	The sediment was suspended in 0.85% (w/v) NaCl solution and the serially diluted solution was spread on MA. BPTF-M20T was isolated from the MA plate that was incubated for at least 1 week at 25 C and cultivated routinely on MA at 30 C unless stated otherwise.

[Ref.: #67771]	Sample type/isolated from	From mud flat
[Ref.: #67771]	Geographic location (country and/or sea, region)	Anmyundo, Taean-gun, Chungcheongnam-do
[Ref.: #67771]	Country	Republic of Korea
[Ref.: #67771]	Country ISO 3 Code	KOR
[Ref.: #67771]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Sediment
#Environmental	#Terrestrial	#Tidal flat

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence MH497062 (>99% sequence identity) for Pseudoruegeria from Microbeatlas

Aquatic Samples	2823
Soil Samples	86
Animal Samples	166
Plant Samples	21
Total Samples	3096

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66745]	Pseudoruegeria sp. BPTF-M20 16S ribosomal RNA gene, partial sequence	MH497062	1392	ENA	2231601 tax ID

[Ref.: #66745]

GC-content

63.2 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #66745]

Culture collection no.

KACC 19614, KCTC 62422, NBRC 113188

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Pseudoruegeria insulae sp. nov., isolated from a tidal flat.	Park S, Park JM, Yoon JH	Int J Syst Evol Microbiol	10.1099/ijsem.0.003035	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66745

Sooyeon Park, Ji-Min Park and Jung-Hoon Yoon: Pseudoruegeria insulae sp. nov., isolated from a tidal flat. IJSEM 68: 3587 - 3592 2018 ( DOI 10.1099/ijsem.0.003035 , PubMed 30239328 )

#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Pseudoruegeria insulae in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF