Strain identifier

BacDive ID: 159803

Type strain: Yes

Species: Pseudoruegeria insulae

Strain Designation: BPTF-M20

Strain history: <- Jung-Hoon YOON, Sungkyunkwan Univ.

NCBI tax ID(s): 2231601 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 66745

BacDive-ID: 159803

keywords: 16S sequence, Bacteria, aerobe, psychrophilic, Gram-negative, rod-shaped, colony-forming

description: Pseudoruegeria insulae BPTF-M20 is an aerobe, psychrophilic, Gram-negative bacterium that forms circular colonies and was isolated from a tidal flat sediment.

NCBI tax id

  • NCBI tax id: 2231601
  • Matching level: species

strain history

  • @ref: 67771
  • history: <- Jung-Hoon YOON, Sungkyunkwan Univ.

doi: 10.13145/bacdive159803.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Rhodobacterales
  • family: Roseobacteraceae
  • genus: Pseudoruegeria
  • species: Pseudoruegeria insulae
  • full scientific name: Pseudoruegeria insulae Park et al. 2018

@ref: 66745

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhodobacterales

family: Rhodobacteraceae

genus: Pseudoruegeria

species: Pseudoruegeria insulae

strain designation: BPTF-M20

type strain: yes

Morphology

cell morphology

  • @ref: 66745
  • gram stain: negative
  • cell length: 0.4-10.0 µm
  • cell width: 0.4-0.8 µm
  • cell shape: rod-shaped

colony morphology

  • @ref: 66745
  • colony size: 0.5-1 mm
  • colony color: greyish yellow
  • colony shape: circular
  • incubation period: 5 days
  • medium used: Marine agar (MA)

Culture and growth conditions

culture medium

  • @ref: 66745
  • name: Marine agar (MA)
  • growth: yes

culture temp

@refgrowthtypetemperaturerange
66745nogrowth4psychrophilic
66745positiveoptimum30mesophilic
66745nogrowth37mesophilic
66745positivegrowth10-35
67771positivegrowth30mesophilic

culture pH

@refabilitytypepH
66745nogrowth5.5
66745positivegrowth6
66745positiveoptimum7-8

Physiology and metabolism

oxygen tolerance

  • @ref: 67771
  • oxygen tolerance: aerobe

spore formation

  • @ref: 66745
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
66745NaClpositivegrowth1.5-5.0 %(w/v)
66745NaClpositiveoptimum2.0-3.0 %(w/v)

observation

@refobservation
66745Mg2+ ions are required for growth.
66745The predominant ubiquinone is Q-10.
66745The major polar lipids are phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, one unidentified aminolipid and one unidentified glycolipid.

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6674512936D-galactose-carbon source
6674515589L-malate-carbon source
6674515824D-fructose-carbon source
6674516024D-mannose-carbon source
6674516150benzoate-carbon source
6674517057cellobiose-carbon source
6674517306maltose-carbon source
6674517634D-glucose-carbon source
6674517814salicin-carbon source
6674517992sucrose-carbon source
6674527082trehalose-carbon source
6674529985L-glutamate-carbon source
6674530849L-arabinose-carbon source
6674532954sodium acetate-carbon source
6674553258sodium citrate-carbon source
6674562965sodium formate-carbon source
6674565327D-xylose-carbon source
6674512936D-galactose-energy source
6674515589L-malate-energy source
6674515824D-fructose-energy source
6674516024D-mannose-energy source
6674516150benzoate-energy source
6674517057cellobiose-energy source
6674517306maltose-energy source
6674517634D-glucose-energy source
6674517814salicin-energy source
6674517992sucrose-energy source
6674527082trehalose-energy source
6674529985L-glutamate-energy source
6674530849L-arabinose-energy source
6674532954sodium acetate-energy source
6674553258sodium citrate-energy source
6674562965sodium formate-energy source
6674565327D-xylose-energy source
667454853esculin-hydrolysis
667455291gelatin-hydrolysis
6674515318xanthine-hydrolysis
6674516199urea-hydrolysis
6674517368hypoxanthine-hydrolysis
6674517895L-tyrosine-hydrolysis
6674528017starch-hydrolysis
6674553426tween 80-hydrolysis
66745casein-hydrolysis

enzymes

@refvalueactivityec
66745acid phosphatase-3.1.3.2
66745alpha-chymotrypsin-3.4.21.1
66745alpha-fucosidase-3.2.1.51
66745alpha-galactosidase-3.2.1.22
66745alpha-mannosidase-3.2.1.24
66745beta-galactosidase-3.2.1.23
66745beta-glucosidase-3.2.1.21
66745beta-glucuronidase-3.2.1.31
66745cystine arylamidase-3.4.11.3
66745lipase (C 14)-
66745N-acetyl-beta-glucosaminidase-3.2.1.52
66745trypsin-3.4.21.4
66745valine arylamidase-
66745alkaline phosphatase+3.1.3.1
66745alpha-glucosidase+3.2.1.20
66745catalase+1.11.1.6
66745cytochrome oxidase+1.9.3.1
66745esterase (C 4)+
66745esterase Lipase (C 8)+
66745leucine arylamidase+3.4.11.1
66745naphthol-AS-BI-phosphohydrolase+
66745nitrate reductase+1.7.99.4

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    66745C20:1 w7c0.7
    6674511-methyl C18:1 w7c1.2
    66745C18:01.2
    66745C16:04.6
    66745C18:1 w7c90.7
  • type of FA analysis: whole cell analysis
  • incubation medium: Marine Agar
  • incubation temperature: 30
  • incubation time: 3
  • software version: Sherlock 6.2B
  • library/peak naming table: TSBA6
  • system: MIS MIDI

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentenrichment cultureenrichment culture durationenrichment culture temperatureisolation procedure
66745a tidal flat sedimentAnmyeon island on the Yellow SeaRepublic of KoreaKORAsiamarine agar 2216 (MA; BD Difco).1 week25The sediment was suspended in 0.85% (w/v) NaCl solution and the serially diluted solution was spread on MA. BPTF-M20T was isolated from the MA plate that was incubated for at least 1 week at 25 C and cultivated routinely on MA at 30 C unless stated otherwise.
67771From mud flatAnmyundo, Taean-gun, Chungcheongnam-doRepublic of KoreaKORAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Sediment
#Environmental#Terrestrial#Tidal flat

taxonmaps

  • @ref: 69479
  • File name: preview.99_119651.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_19;96_1697;97_2031;98_80681;99_119651&stattab=map
  • Last taxonomy: Pseudoruegeria
  • 16S sequence: MH497062
  • Sequence Identity:
  • Total samples: 3096
  • soil counts: 86
  • aquatic counts: 2823
  • animal counts: 166
  • plant counts: 21

Sequence information

16S sequences

  • @ref: 66745
  • description: Pseudoruegeria sp. BPTF-M20 16S ribosomal RNA gene, partial sequence
  • accession: MH497062
  • length: 1392
  • database: ena
  • NCBI tax ID: 2231601

GC content

  • @ref: 66745
  • GC-content: 63.2
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 66745

culture collection no.: KACC 19614, KCTC 62422, NBRC 113188

literature

  • topic: Phylogeny
  • Pubmed-ID: 30239328
  • title: Pseudoruegeria insulae sp. nov., isolated from a tidal flat.
  • authors: Park S, Park JM, Yoon JH
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.003035
  • year: 2018
  • mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Nucleic Acid Hybridization, Phosphatidylglycerols/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Rhodobacteraceae/*classification/genetics/isolation & purification, Seawater/*microbiology, Sequence Analysis, DNA, Ubiquinone/analogs & derivatives/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
66745Sooyeon Park, Ji-Min Park and Jung-Hoon YoonPseudoruegeria insulae sp. nov., isolated from a tidal flat10.1099/ijsem.0.003035IJSEM 68: 3587-3592 201830239328
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/