Strain identifier
BacDive ID: 159803
Type strain:
Species: Pseudoruegeria insulae
Strain Designation: BPTF-M20
Strain history: <- Jung-Hoon YOON, Sungkyunkwan Univ.
NCBI tax ID(s): 2231601 (species)
General
@ref: 66745
BacDive-ID: 159803
keywords: 16S sequence, Bacteria, aerobe, psychrophilic, Gram-negative, rod-shaped, colony-forming
description: Pseudoruegeria insulae BPTF-M20 is an aerobe, psychrophilic, Gram-negative bacterium that forms circular colonies and was isolated from a tidal flat sediment.
NCBI tax id
- NCBI tax id: 2231601
- Matching level: species
strain history
- @ref: 67771
- history: <- Jung-Hoon YOON, Sungkyunkwan Univ.
doi: 10.13145/bacdive159803.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Alphaproteobacteria
- order: Rhodobacterales
- family: Roseobacteraceae
- genus: Pseudoruegeria
- species: Pseudoruegeria insulae
- full scientific name: Pseudoruegeria insulae Park et al. 2018
@ref: 66745
domain: Bacteria
phylum: Proteobacteria
class: Alphaproteobacteria
order: Rhodobacterales
family: Rhodobacteraceae
genus: Pseudoruegeria
species: Pseudoruegeria insulae
strain designation: BPTF-M20
type strain: yes
Morphology
cell morphology
- @ref: 66745
- gram stain: negative
- cell length: 0.4-10.0 µm
- cell width: 0.4-0.8 µm
- cell shape: rod-shaped
colony morphology
- @ref: 66745
- colony size: 0.5-1 mm
- colony color: greyish yellow
- colony shape: circular
- incubation period: 5 days
- medium used: Marine agar (MA)
Culture and growth conditions
culture medium
- @ref: 66745
- name: Marine agar (MA)
- growth: yes
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
66745 | no | growth | 4 | psychrophilic |
66745 | positive | optimum | 30 | mesophilic |
66745 | no | growth | 37 | mesophilic |
66745 | positive | growth | 10-35 | |
67771 | positive | growth | 30 | mesophilic |
culture pH
@ref | ability | type | pH |
---|---|---|---|
66745 | no | growth | 5.5 |
66745 | positive | growth | 6 |
66745 | positive | optimum | 7-8 |
Physiology and metabolism
oxygen tolerance
- @ref: 67771
- oxygen tolerance: aerobe
spore formation
- @ref: 66745
- spore formation: no
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
66745 | NaCl | positive | growth | 1.5-5.0 %(w/v) |
66745 | NaCl | positive | optimum | 2.0-3.0 %(w/v) |
observation
@ref | observation |
---|---|
66745 | Mg2+ ions are required for growth. |
66745 | The predominant ubiquinone is Q-10. |
66745 | The major polar lipids are phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, one unidentified aminolipid and one unidentified glycolipid. |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
66745 | 12936 | D-galactose | - | carbon source |
66745 | 15589 | L-malate | - | carbon source |
66745 | 15824 | D-fructose | - | carbon source |
66745 | 16024 | D-mannose | - | carbon source |
66745 | 16150 | benzoate | - | carbon source |
66745 | 17057 | cellobiose | - | carbon source |
66745 | 17306 | maltose | - | carbon source |
66745 | 17634 | D-glucose | - | carbon source |
66745 | 17814 | salicin | - | carbon source |
66745 | 17992 | sucrose | - | carbon source |
66745 | 27082 | trehalose | - | carbon source |
66745 | 29985 | L-glutamate | - | carbon source |
66745 | 30849 | L-arabinose | - | carbon source |
66745 | 32954 | sodium acetate | - | carbon source |
66745 | 53258 | sodium citrate | - | carbon source |
66745 | 62965 | sodium formate | - | carbon source |
66745 | 65327 | D-xylose | - | carbon source |
66745 | 12936 | D-galactose | - | energy source |
66745 | 15589 | L-malate | - | energy source |
66745 | 15824 | D-fructose | - | energy source |
66745 | 16024 | D-mannose | - | energy source |
66745 | 16150 | benzoate | - | energy source |
66745 | 17057 | cellobiose | - | energy source |
66745 | 17306 | maltose | - | energy source |
66745 | 17634 | D-glucose | - | energy source |
66745 | 17814 | salicin | - | energy source |
66745 | 17992 | sucrose | - | energy source |
66745 | 27082 | trehalose | - | energy source |
66745 | 29985 | L-glutamate | - | energy source |
66745 | 30849 | L-arabinose | - | energy source |
66745 | 32954 | sodium acetate | - | energy source |
66745 | 53258 | sodium citrate | - | energy source |
66745 | 62965 | sodium formate | - | energy source |
66745 | 65327 | D-xylose | - | energy source |
66745 | 4853 | esculin | - | hydrolysis |
66745 | 5291 | gelatin | - | hydrolysis |
66745 | 15318 | xanthine | - | hydrolysis |
66745 | 16199 | urea | - | hydrolysis |
66745 | 17368 | hypoxanthine | - | hydrolysis |
66745 | 17895 | L-tyrosine | - | hydrolysis |
66745 | 28017 | starch | - | hydrolysis |
66745 | 53426 | tween 80 | - | hydrolysis |
66745 | casein | - | hydrolysis |
enzymes
@ref | value | activity | ec |
---|---|---|---|
66745 | acid phosphatase | - | 3.1.3.2 |
66745 | alpha-chymotrypsin | - | 3.4.21.1 |
66745 | alpha-fucosidase | - | 3.2.1.51 |
66745 | alpha-galactosidase | - | 3.2.1.22 |
66745 | alpha-mannosidase | - | 3.2.1.24 |
66745 | beta-galactosidase | - | 3.2.1.23 |
66745 | beta-glucosidase | - | 3.2.1.21 |
66745 | beta-glucuronidase | - | 3.2.1.31 |
66745 | cystine arylamidase | - | 3.4.11.3 |
66745 | lipase (C 14) | - | |
66745 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
66745 | trypsin | - | 3.4.21.4 |
66745 | valine arylamidase | - | |
66745 | alkaline phosphatase | + | 3.1.3.1 |
66745 | alpha-glucosidase | + | 3.2.1.20 |
66745 | catalase | + | 1.11.1.6 |
66745 | cytochrome oxidase | + | 1.9.3.1 |
66745 | esterase (C 4) | + | |
66745 | esterase Lipase (C 8) | + | |
66745 | leucine arylamidase | + | 3.4.11.1 |
66745 | naphthol-AS-BI-phosphohydrolase | + | |
66745 | nitrate reductase | + | 1.7.99.4 |
fatty acid profile
fatty acids
@ref fatty acid percentage 66745 C20:1 w7c 0.7 66745 11-methyl C18:1 w7c 1.2 66745 C18:0 1.2 66745 C16:0 4.6 66745 C18:1 w7c 90.7 - type of FA analysis: whole cell analysis
- incubation medium: Marine Agar
- incubation temperature: 30
- incubation time: 3
- software version: Sherlock 6.2B
- library/peak naming table: TSBA6
- system: MIS MIDI
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent | enrichment culture | enrichment culture duration | enrichment culture temperature | isolation procedure |
---|---|---|---|---|---|---|---|---|---|
66745 | a tidal flat sediment | Anmyeon island on the Yellow Sea | Republic of Korea | KOR | Asia | marine agar 2216 (MA; BD Difco). | 1 week | 25 | The sediment was suspended in 0.85% (w/v) NaCl solution and the serially diluted solution was spread on MA. BPTF-M20T was isolated from the MA plate that was incubated for at least 1 week at 25 C and cultivated routinely on MA at 30 C unless stated otherwise. |
67771 | From mud flat | Anmyundo, Taean-gun, Chungcheongnam-do | Republic of Korea | KOR | Asia |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Environmental | #Aquatic | #Sediment |
#Environmental | #Terrestrial | #Tidal flat |
taxonmaps
- @ref: 69479
- File name: preview.99_119651.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_19;96_1697;97_2031;98_80681;99_119651&stattab=map
- Last taxonomy: Pseudoruegeria
- 16S sequence: MH497062
- Sequence Identity:
- Total samples: 3096
- soil counts: 86
- aquatic counts: 2823
- animal counts: 166
- plant counts: 21
Sequence information
16S sequences
- @ref: 66745
- description: Pseudoruegeria sp. BPTF-M20 16S ribosomal RNA gene, partial sequence
- accession: MH497062
- length: 1392
- database: ena
- NCBI tax ID: 2231601
GC content
- @ref: 66745
- GC-content: 63.2
- method: high performance liquid chromatography (HPLC)
External links
@ref: 66745
culture collection no.: KACC 19614, KCTC 62422, NBRC 113188
literature
- topic: Phylogeny
- Pubmed-ID: 30239328
- title: Pseudoruegeria insulae sp. nov., isolated from a tidal flat.
- authors: Park S, Park JM, Yoon JH
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijsem.0.003035
- year: 2018
- mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Nucleic Acid Hybridization, Phosphatidylglycerols/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Rhodobacteraceae/*classification/genetics/isolation & purification, Seawater/*microbiology, Sequence Analysis, DNA, Ubiquinone/analogs & derivatives/chemistry
- topic2: Transcriptome
Reference
@id | authors | title | doi/url | journal | pubmed |
---|---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | ||
66745 | Sooyeon Park, Ji-Min Park and Jung-Hoon Yoon | Pseudoruegeria insulae sp. nov., isolated from a tidal flat | 10.1099/ijsem.0.003035 | IJSEM 68: 3587-3592 2018 | 30239328 |
67771 | Curators of the KCTC | https://kctc.kribb.re.kr/En/Kctc | |||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ |