Name: Microbispora triticiradicis NEAU-HRDPA2-9
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 159804

Type strain:

Species: Microbispora triticiradicis

Strain Designation: NEAU-HRDPA2-9

Culture col. no.: CGMCC 4.7399, DSM 104649, JCM 33405

Strain history: CGMCC 4.7399 <-- W. Xiang and X. Wang; Northeast Agric. Univ., China; NEAU-HRDPA2-9.

NCBI tax ID(s): 2200763 (species)

SI-ID 401761

DSM 104649

Section

Microbispora triticiradicis NEAU-HRDPA2-9 is an aerobe, spore-forming, mesophilic bacterium that was isolated from the root of wheat .

Gram-positive
oval-shaped
spore-forming
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Streptosporangiales*
	Family	*Streptosporangiaceae*
	Genus	*Microbispora*
	Species	**Microbispora triticiradicis**
	Full Scientific Name (LPSN)	Microbispora triticiradicis Han et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #66750]	Gram stain	positive

[Ref.: #66750]	Cell length	1.14-1.38 µm

[Ref.: #66750]	Cell width	0.96-1.30 µm

[Ref.: #66750]	Cell shape	oval-shaped

[Ref.: #66750]	Motility	no

[Ref.: #66750]	Colony color	pale yellowish pink to dark reddish brown
[Ref.: #66750]	Cultivation medium used	ISP

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Pigment production

Information on culture and growth conditions Culture and growth conditions

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Culture medium

Bennet's agar media

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Culture medium growth

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Culture medium

ISP

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Culture medium growth

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Culture medium

ISP 1

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Culture medium growth

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Culture medium

ISP 2

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Culture medium growth

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Culture medium

ISP 3

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Culture medium growth

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Culture medium

ISP 4

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Culture medium growth

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Culture medium

Czapek's agar

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Culture medium growth

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Culture medium

ISP 5

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Culture medium growth

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Culture medium

ISP 6

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Culture medium growth

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Culture medium

ISP 7

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Culture medium growth

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Culture medium

Nutrient agar (NA)

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Culture medium growth

		Temperature
[Ref.: #66750]	optimum	28 °C
[Ref.: #66750]	growth	20-45 °C
[Ref.: #67770]	growth	28 °C

[Ref.: #66750]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #66750]			optimum	7
[Ref.: #66750]			growth	5-10

Information on physiology and metabolism Physiology and metabolism

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Oxygen tolerance

aerobe

[Ref.: #66750]	Spore description	forms longitudinal pairs spores
[Ref.: #66750]	Type of spore	spore
[Ref.: #66750]	Ability of spore formation	yes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #66750]		NaCl		growth	0-3.5	%(w/v)
[Ref.: #66750]		NaCl		optimum	0-1	%(w/v)

[Ref.: #66750]	Observation	The cell wall contains meso-diaminopimelic acid as the diagnostic diamino acid and the whole-cell hydrolysate contains madurose and glucose.
[Ref.: #66750]	Observation	The major menaquinones are MK-9(H0), MK-9(H2) and MK-9(H4).
[Ref.: #66750]	Observation	The polar lipids profile contains diphosphatidylglycerol, ninhydrin-positive glycophospholipid, phosphatidylethanolamine, two phosphatidylinositol mannosides, phosphatidylmonomethylethanolamine and an unidentified glycolipid.
[Ref.: #67770]	Observation	quinones: MK-9(H₂), MK-9(H₀), MK-9(H₄)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #66750]	cellulose ChEBI	+	degradation
[Ref.: #66750]	creatine ChEBI	-	carbon source
[Ref.: #66750]	D-fructose ChEBI	+	carbon source
[Ref.: #66750]	D-galactose ChEBI	+	carbon source
[Ref.: #66750]	D-glucose ChEBI	+	carbon source
[Ref.: #66750]	D-mannitol ChEBI	+	carbon source
[Ref.: #66750]	D-mannose ChEBI	+	carbon source
[Ref.: #66750]	D-ribose ChEBI	-	carbon source
[Ref.: #66750]	D-sorbitol ChEBI	+	carbon source
[Ref.: #66750]	D-xylose ChEBI	+	carbon source
[Ref.: #66750]	esculin ChEBI	+	hydrolysis
[Ref.: #66750]	glycine ChEBI	-	nitrogen source
[Ref.: #66750]	hydrogen sulfide ChEBI	-	assimilation
[Ref.: #66750]	L-alanine ChEBI	-	nitrogen source
[Ref.: #66750]	L-arabinose ChEBI	-	carbon source
[Ref.: #66750]	L-arginine ChEBI	+	nitrogen source
[Ref.: #66750]	L-asparagine ChEBI	+	nitrogen source
[Ref.: #66750]	L-aspartate ChEBI	+	nitrogen source
[Ref.: #66750]	L-glutamate ChEBI	+	nitrogen source
[Ref.: #66750]	L-glutamine ChEBI	-	nitrogen source
[Ref.: #66750]	L-proline ChEBI	+	nitrogen source
[Ref.: #66750]	L-rhamnose ChEBI	-	carbon source
[Ref.: #66750]	L-serine ChEBI	+	nitrogen source
[Ref.: #66750]	L-threonine ChEBI	-	nitrogen source
[Ref.: #66750]	L-tyrosine ChEBI	-	nitrogen source
[Ref.: #66750]	lactose ChEBI	-	carbon source
[Ref.: #66750]	maltose ChEBI	+	carbon source
[Ref.: #66750]	myo-inositol ChEBI	-	carbon source
[Ref.: #66750]	raffinose ChEBI	-	carbon source
[Ref.: #66750]	starch ChEBI	-	hydrolysis
[Ref.: #66750]	sucrose ChEBI	+	carbon source
[Ref.: #66750]	tween 20 ChEBI	+	degradation
[Ref.: #66750]	tween 80 ChEBI	-	degradation
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #66750]	catalase	+	1.11.1.6
[Ref.: #66750]	nitrate reductase	-	1.7.99.4
[Ref.: #66750]	urease	+	3.5.1.5

Fatty acid profile

Metadata FA analysis

Reference

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Fatty acid	Percentage
C16:0 iso	30.7
C16:0	27.9
10-methyl C17:0	19.2
C16:1 w7c	6.9
C17:0 anteiso	4.8
C18:1 w9c	3.9
C17:1 w7c	3.8
C17:0 iso	1.5
C15:0	1.3

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #66750]	Sample type/isolated from	the root of wheat (Triticum aestivum L.)
[Ref.: #66750]	Host species	Triticum aestivum
[Ref.: #66750]	Geographic location (country and/or sea, region)	Zhumadian, Henan Province
[Ref.: #66750]	Country	China
[Ref.: #66750]	Country ISO 3 Code	CHN
[Ref.: #66750]	Continent	Asia
[Ref.: #66750]	Enrichment culture	Dulcitol-proline agar
[Ref.: #66750]	Enrichment culture composition	supplemented with cycloheximide (50 mg/L) and nalidixic acid (20 mg/L).
[Ref.: #66750]	Enrichment culture duration	28 days
[Ref.: #66750]	Enrichment culture temperature	28 ̊C

[Ref.: #67770]	Sample type/isolated from	Roots of wheat (Triticum aestivum L.) from Zhumadian
[Ref.: #67770]	Host species	Triticum aestivum
[Ref.: #67770]	Geographic location (country and/or sea, region)	Henan Province, Central China
[Ref.: #67770]	Country	China
[Ref.: #67770]	Country ISO 3 Code	CHN
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Root (Rhizome)

What are isolation sources categories?

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Global distribution of 16S sequence MF969266 (>99% sequence identity) for Microbispora from Microbeatlas

Aquatic Samples	10
Soil Samples	327
Animal Samples	14
Plant Samples	113
Total Samples	464

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66750]	Microbispora sp. strain NEAU-HRDPA2-9 16S ribosomal RNA gene, partial sequence	MF969266	1515	ENA	2200763 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Microbispora triticiradicis NEAU-HRDPA2-9	GCA_003260025	contig	GenBank	2200763 tax ID
[Ref.: #66792]	Microbispora triticiradicis NEAU-HRDPA2-9	2856741275	draft	JGI IMG/M	2200763 tax ID	*

[Ref.: #66750]	GC-content	71.7 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #67770]	GC-content	71.7 mol%	genome sequence analysis

Availability in culture collections External links

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Culture collection no.

CGMCC 4.7399, DSM 104649, JCM 33405

[Ref.: #112172]

SI-ID 401761

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Microbispora tritici sp. nov., a novel actinomycete isolated from a root of wheat (Triticum aestivum L.).	Han C, Zhao J, Yu B, Shi H, Zhang C, Guo X, Xiang W, Wang X	Antonie Van Leeuwenhoek	10.1007/s10482-019-01246-y	2019	*
Phylogeny	Microbispora triticiradicis sp. nov., a novel actinomycete isolated from the root of wheat (Triticum aestivum L.).	Han C, Tian Y, Zhao J, Yu Z, Jiang S, Guo X, Xiang W, Wang X	Int J Syst Evol Microbiol	10.1099/ijsem.0.003040	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66750

Chuanyu Han, Yuanyuan Tian, Junwei Zhao, Zhiyin Yu, Shanwen Jiang, Xiaowei Guo, Wensheng Xiang and Xiangjing Wang: Microbispora triticiradicis sp. nov., a novel actinomycete isolated from the root of wheat (Triticum aestivum L.). IJSEM 68: 3600 - 3605 2018 ( DOI 10.1099/ijsem.0.003040 , PubMed 30247118 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#112172

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID401761.1 )

* These data were automatically processed and therefore are not curated

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