Name: Rhodococcus olei Ktm-20
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 158727

Type strain:

Species: Rhodococcus olei

Strain Designation: Ktm-20

Culture col. no.: KEMB 9005-695, KACC 19390, JCM 32206

Strain history: J. Kim; Kyonggi Univ., South Korea; Ktm-20.

NCBI tax ID(s): 2161675 (species)

Section

Rhodococcus olei Ktm-20 is an aerobe, Gram-positive prokaryote that was isolated from an oil-contaminated soil sample of Biratnagar during a study of oil-degrading bacteria.

Gram-positive
aerobe
16S sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Genus	*Rhodococcus*
	Species	**Rhodococcus olei**
	Full Scientific Name (LPSN)	Rhodococcus olei Chaudhary and Kim 2018

Information on morphological and physiological properties Morphology

[Ref.: #65475]	Gram stain	positive

[Ref.: #65475]	Motility	no

[Ref.: #65475]	Colony size	0.1-1.5 mm
[Ref.: #65475]	Colony color	creamy-white
[Ref.: #65475]	Colony shape	circular
[Ref.: #65475]	Incubation period	4 days
[Ref.: #65475]	Cultivation medium used	Trypticase soy agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #65475]

Culture medium

Trypticase soy agar

[Ref.: #65475]

Culture medium growth

[Ref.: #65475]

Culture medium

Reasoner's 2A agar

[Ref.: #65475]

Culture medium growth

[Ref.: #65475]

Culture medium

Nutrient agar (NA)

[Ref.: #65475]

Culture medium growth

[Ref.: #65475]

Culture medium

veal infusion agar

[Ref.: #65475]

Culture medium growth

		Temperature
[Ref.: #65475]	growth	15-37 °C
[Ref.: #65475]	optimum	25-32 °C
[Ref.: #67770]	growth	28 °C

[Ref.: #65475]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #65475]			optimum	6.5-8.5
[Ref.: #65475]			growth	5.5-10.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #65475]

Oxygen tolerance

aerobe

[Ref.: #65475]

Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #65475]		NaCl		growth	0-2	%
[Ref.: #65475]		NaCl		optimum	0	%

[Ref.: #65475]	Observation	rod-coccus growth cycle
[Ref.: #65475]	Observation	MK-8(H2) is the only respiratory quinone
[Ref.: #65475]	Observation	major polar lipids present are phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside
[Ref.: #65475]	Observation	contains meso-diaminopimelic, galactose, glucose, arabinose and ribose in the cell wall
[Ref.: #65475]	Observation	mycolic acids are present
[Ref.: #67770]	Observation	quinones: MK-8(H₂)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #65475]	3-hydroxybenzoate ChEBI	+	assimilation
[Ref.: #65475]	3-hydroxybutyrate ChEBI	-	assimilation
[Ref.: #65475]	4-hydroxybenzoate ChEBI	+	assimilation
[Ref.: #65475]	adipate ChEBI	-	assimilation
[Ref.: #65475]	casein	-	hydrolysis
[Ref.: #65475]	cellulose ChEBI	+	hydrolysis
[Ref.: #65475]	chitin ChEBI	+	hydrolysis
[Ref.: #65475]	D-glucose ChEBI	+	assimilation
[Ref.: #65475]	D-mannitol ChEBI	+	assimilation
[Ref.: #65475]	D-mannose ChEBI	+	assimilation
[Ref.: #65475]	D-ribose ChEBI	-	assimilation
[Ref.: #65475]	D-sorbitol ChEBI	-	assimilation
[Ref.: #65475]	decanoate ChEBI	-	assimilation
[Ref.: #65475]	dna ChEBI	-	hydrolysis
[Ref.: #65475]	esculin ChEBI	+	hydrolysis
[Ref.: #65475]	gelatin ChEBI	-	fermentation
[Ref.: #65475]	gelatin ChEBI	-	hydrolysis
[Ref.: #65475]	glucose ChEBI	-	fermentation
[Ref.: #65475]	glycogen ChEBI	-	assimilation
[Ref.: #65475]	itaconate ChEBI	-	assimilation
[Ref.: #65475]	L-alanine ChEBI	-	assimilation
[Ref.: #65475]	L-arabinose ChEBI	-	assimilation
[Ref.: #65475]	L-arabinose ChEBI	+	assimilation
[Ref.: #65475]	L-arginine ChEBI	-	fermentation
[Ref.: #65475]	L-fucose ChEBI	-	assimilation
[Ref.: #65475]	L-histidine ChEBI	+	assimilation
[Ref.: #65475]	L-proline ChEBI	-	assimilation
[Ref.: #65475]	L-rhamnose ChEBI	-	assimilation
[Ref.: #65475]	L-serine ChEBI	-	assimilation
[Ref.: #65475]	lactate ChEBI	+	assimilation
[Ref.: #65475]	malate ChEBI	+	assimilation
[Ref.: #65475]	maltose ChEBI	+	assimilation
[Ref.: #65475]	melibiose ChEBI	+	assimilation
[Ref.: #65475]	myo-inositol ChEBI	-	assimilation
[Ref.: #65475]	N-acetylglucosamine ChEBI	-	assimilation
[Ref.: #65475]	nitrate ChEBI	-	reduction
[Ref.: #65475]	phenylacetate ChEBI	+	assimilation
[Ref.: #65475]	potassium 2-dehydro-D-gluconate	-	assimilation
[Ref.: #65475]	potassium 5-dehydro-D-gluconate	-	assimilation
[Ref.: #65475]	potassium gluconate ChEBI	-	assimilation
[Ref.: #65475]	propionate ChEBI	+	assimilation
[Ref.: #65475]	salicin ChEBI	+	assimilation
[Ref.: #65475]	sodium acetate ChEBI	+	assimilation
[Ref.: #65475]	sodium citrate ChEBI	-	assimilation
[Ref.: #65475]	sodium malonate ChEBI	-	assimilation
[Ref.: #65475]	starch ChEBI	-	hydrolysis
[Ref.: #65475]	suberic acid ChEBI	-	assimilation
[Ref.: #65475]	sucrose ChEBI	+	assimilation
[Ref.: #65475]	tween 40 ChEBI	+	hydrolysis
[Ref.: #65475]	tween 80 ChEBI	+	hydrolysis
[Ref.: #65475]	tyrosine ChEBI	-	hydrolysis
[Ref.: #65475]	urea ChEBI	-	fermentation
[Ref.: #65475]	valerate ChEBI	+	assimilation
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #65475]		indole ChEBI	no
[Ref.: #65475]		sulfide ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #65475]	acid phosphatase	+	3.1.3.2
[Ref.: #65475]	alkaline phosphatase	+	3.1.3.1
[Ref.: #65475]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #65475]	alpha-fucosidase	-	3.2.1.51
[Ref.: #65475]	alpha-galactosidase	-	3.2.1.22
[Ref.: #65475]	alpha-glucosidase	+	3.2.1.20
[Ref.: #65475]	alpha-mannosidase	-	3.2.1.24
[Ref.: #65475]	beta-galactosidase	-	3.2.1.23
[Ref.: #65475]	beta-galactosidase	+	3.2.1.23
[Ref.: #65475]	beta-glucosidase	+	3.2.1.21
[Ref.: #65475]	beta-glucuronidase	-	3.2.1.31
[Ref.: #65475]	catalase	+	1.11.1.6
[Ref.: #65475]	cystine arylamidase	-	3.4.11.3
[Ref.: #65475]	cytochrome oxidase	-	1.9.3.1
[Ref.: #65475]	esterase (C 4)	-
[Ref.: #65475]	esterase Lipase (C 8)	+
[Ref.: #65475]	leucine arylamidase	+	3.4.11.1
[Ref.: #65475]	lipase (C 14)	+
[Ref.: #65475]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #65475]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #65475]	trypsin	-	3.4.21.4
[Ref.: #65475]	valine arylamidase	+
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #65475]

Fatty acid	Percentage
C16:0	35.1
C16:1ω7c and/or C16:1ω6c	23.2
C18:1ω9c	14.8
C14:0	5.6
C17:1ω8c	4.8
C17:0	4.7
C18:0 10-methyl	3.7
C18:0	2.8
C19:0	1
iso-C17:1ω9c and/or C16:0 10-methyl	1
C17:0 10-methyl	0.8
C15:1ω6c	0.7
C19:1ω11c and/or C19:1ω9c	0.6
C14:1ω5c	0.2

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #65475]	Sample type/isolated from	an oil-contaminated soil sample of Biratnagar during a study of oil-degrading bacteria
[Ref.: #65475]	Geographic location (country and/or sea, region)	Morang
[Ref.: #65475]	Country	Nepal
[Ref.: #65475]	Country ISO 3 Code	NPL
[Ref.: #65475]	Continent	Asia
[Ref.: #65475]	Isolation/enrichment procedure	modified culture technique with six-well polycarbonate Transwell plates as described previously

[Ref.: #67770]	Sample type/isolated from	Oil-contaminated soil of Biratnagar
[Ref.: #67770]	Geographic location (country and/or sea, region)	Morang
[Ref.: #67770]	Country	Nepal
[Ref.: #67770]	Country ISO 3 Code	NPL
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Engineered	#Contamination	#Oil (Fuel)
#Environmental	#Terrestrial	#Soil

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #65475]	16S rRNA gene sequence	MF405107		GenBank

[Ref.: #65475]

GC-content

70.9 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #65475]

Culture collection no.

KEMB 9005-695, KACC 19390, JCM 32206

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Rhodococcus olei sp. nov., with the ability to degrade petroleum oil, isolated from oil-contaminated soil.	Chaudhary DK, Kim J	Int J Syst Evol Microbiol	10.1099/ijsem.0.002750	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65475

Dhiraj Kumar Chaudhary, Jaisoo Kim: Rhodococcus olei sp. nov., with the ability to degrade petroleum oil, isolated from oil-contaminated soil. IJSEM 68: 1749 - 1756 2018 ( DOI 10.1099/ijsem.0.002750 , PubMed 29620494 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;
* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Rhodococcus olei in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF