Strain identifier
BacDive ID: 158727
Type strain:
Species: Rhodococcus olei
Strain Designation: Ktm-20
Strain history: J. Kim; Kyonggi Univ., South Korea; Ktm-20.
NCBI tax ID(s): 2161675 (species)
General
@ref: 65475
BacDive-ID: 158727
keywords: 16S sequence, aerobe, Gram-positive
description: Rhodococcus olei Ktm-20 is an aerobe, Gram-positive prokaryote that was isolated from an oil-contaminated soil sample of Biratnagar during a study of oil-degrading bacteria.
NCBI tax id
- NCBI tax id: 2161675
- Matching level: species
strain history
- @ref: 67770
- history: J. Kim; Kyonggi Univ., South Korea; Ktm-20.
doi: 10.13145/bacdive158727.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- genus: Rhodococcus
- species: Rhodococcus olei
- full scientific name: Rhodococcus olei Chaudhary and Kim 2018
@ref: 65475
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Mycobacteriales
family: Nocardiaceae
genus: Rhodococcus
species: Rhodococcus olei
strain designation: Ktm-20
type strain: yes
Morphology
cell morphology
- @ref: 65475
- gram stain: positive
- motility: no
colony morphology
@ref | colony size | colony color | colony shape | incubation period | medium used |
---|---|---|---|---|---|
65475 | |||||
65475 | 0.1-1.5 mm | creamy-white | circular | 4 days | Trypticase soy agar |
Culture and growth conditions
culture medium
@ref | name | growth |
---|---|---|
65475 | Trypticase soy agar | yes |
65475 | Reasoner's 2A agar | yes |
65475 | Nutrient agar (NA) | yes |
65475 | veal infusion agar | yes |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
65475 | positive | growth | 15-37 | |
65475 | positive | optimum | 25-32 | mesophilic |
67770 | positive | growth | 28 | mesophilic |
culture pH
@ref | ability | type | pH | PH range |
---|---|---|---|---|
65475 | positive | growth | 5.5-10.0 | alkaliphile |
65475 | positive | optimum | 6.5-8.5 |
Physiology and metabolism
oxygen tolerance
- @ref: 65475
- oxygen tolerance: aerobe
spore formation
- @ref: 65475
- spore formation: no
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
65475 | NaCl | positive | growth | 0-2 % |
65475 | NaCl | positive | optimum | 0 % |
observation
@ref | observation |
---|---|
65475 | rod-coccus growth cycle |
65475 | MK-8(H2) is the only respiratory quinone |
65475 | major polar lipids present are phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside |
65475 | contains meso-diaminopimelic, galactose, glucose, arabinose and ribose in the cell wall |
65475 | mycolic acids are present |
67770 | quinones: MK-8(H2) |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
65475 | 62968 | cellulose | + | hydrolysis |
65475 | 16193 | 3-hydroxybenzoate | + | assimilation |
65475 | 37054 | 3-hydroxybutyrate | - | assimilation |
65475 | 17879 | 4-hydroxybenzoate | + | assimilation |
65475 | 17128 | adipate | - | assimilation |
65475 | 27689 | decanoate | - | assimilation |
65475 | casein | - | hydrolysis | |
65475 | 17029 | chitin | + | hydrolysis |
65475 | 17634 | D-glucose | + | assimilation |
65475 | 16899 | D-mannitol | + | assimilation |
65475 | 16024 | D-mannose | + | assimilation |
65475 | 16988 | D-ribose | - | assimilation |
65475 | 17924 | D-sorbitol | - | assimilation |
65475 | 16991 | dna | - | hydrolysis |
65475 | 4853 | esculin | + | hydrolysis |
65475 | 5291 | gelatin | - | fermentation |
65475 | 5291 | gelatin | - | hydrolysis |
65475 | 17234 | glucose | - | fermentation |
65475 | 28087 | glycogen | - | assimilation |
65475 | 17240 | itaconate | - | assimilation |
65475 | 16977 | L-alanine | - | assimilation |
65475 | 30849 | L-arabinose | - | assimilation |
65475 | 30849 | L-arabinose | + | assimilation |
65475 | 16467 | L-arginine | - | fermentation |
65475 | 18287 | L-fucose | - | assimilation |
65475 | 15971 | L-histidine | + | assimilation |
65475 | 17203 | L-proline | - | assimilation |
65475 | 62345 | L-rhamnose | - | assimilation |
65475 | 17115 | L-serine | - | assimilation |
65475 | 24996 | lactate | + | assimilation |
65475 | 25115 | malate | + | assimilation |
65475 | 17306 | maltose | + | assimilation |
65475 | 28053 | melibiose | + | assimilation |
65475 | 17268 | myo-inositol | - | assimilation |
65475 | 506227 | N-acetylglucosamine | - | assimilation |
65475 | 17632 | nitrate | - | reduction |
65475 | 18401 | phenylacetate | + | assimilation |
65475 | potassium 2-dehydro-D-gluconate | - | assimilation | |
65475 | potassium 5-dehydro-D-gluconate | - | assimilation | |
65475 | 32032 | potassium gluconate | - | assimilation |
65475 | 17272 | propionate | + | assimilation |
65475 | 17814 | salicin | + | assimilation |
65475 | 32954 | sodium acetate | + | assimilation |
65475 | 53258 | sodium citrate | - | assimilation |
65475 | 62983 | sodium malonate | - | assimilation |
65475 | 28017 | starch | - | hydrolysis |
65475 | 9300 | suberic acid | - | assimilation |
65475 | 17992 | sucrose | + | assimilation |
65475 | 53423 | tween 40 | + | hydrolysis |
65475 | 53426 | tween 80 | + | hydrolysis |
65475 | 18186 | tyrosine | - | hydrolysis |
65475 | 16199 | urea | - | fermentation |
65475 | 31011 | valerate | + | assimilation |
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
65475 | 35581 | indole | no |
65475 | 15138 | sulfide | no |
enzymes
@ref | value | activity | ec |
---|---|---|---|
65475 | catalase | + | 1.11.1.6 |
65475 | cytochrome oxidase | - | 1.9.3.1 |
65475 | leucine arylamidase | + | 3.4.11.1 |
65475 | valine arylamidase | + | |
65475 | acid phosphatase | + | 3.1.3.2 |
65475 | alpha-glucosidase | + | 3.2.1.20 |
65475 | beta-glucosidase | + | 3.2.1.21 |
65475 | alkaline phosphatase | + | 3.1.3.1 |
65475 | esterase Lipase (C 8) | + | |
65475 | lipase (C 14) | + | |
65475 | naphthol-AS-BI-phosphohydrolase | + | |
65475 | esterase (C 4) | - | |
65475 | cystine arylamidase | - | 3.4.11.3 |
65475 | trypsin | - | 3.4.21.4 |
65475 | alpha-chymotrypsin | - | 3.4.21.1 |
65475 | alpha-galactosidase | - | 3.2.1.22 |
65475 | beta-galactosidase | - | 3.2.1.23 |
65475 | beta-glucuronidase | - | 3.2.1.31 |
65475 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
65475 | alpha-mannosidase | - | 3.2.1.24 |
65475 | alpha-fucosidase | - | 3.2.1.51 |
65475 | beta-galactosidase | + | 3.2.1.23 |
fatty acid profile
fatty acids
@ref fatty acid percentage 65475 C14:0 5.6 65475 C16:0 35.1 65475 C17:0 4.7 65475 C18:0 2.8 65475 C19:0 1 65475 C14:1ω5c 0.2 65475 C15:1ω6c 0.7 65475 C17:1ω8c 4.8 65475 C18:1ω9c 14.8 65475 C17:0 10-methyl 0.8 65475 C18:0 10-methyl 3.7 65475 C16:1ω7c and/or C16:1ω6c 23.2 65475 C19:1ω11c and/or C19:1ω9c 0.6 65475 iso-C17:1ω9c and/or C16:0 10-methyl 1 - type of FA analysis: whole cell analysis
- software version: Sherlock 6.0B
- library/peak naming table: TSBA6
- system: MIS MIDI
- instrument: HP 6890 Series GC System; Hewlett Packard
- method/protocol: Sasser, 1990
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent | isolation procedure |
---|---|---|---|---|---|---|
65475 | an oil-contaminated soil sample of Biratnagar during a study of oil-degrading bacteria | Morang | Nepal | NPL | Asia | modified culture technique with six-well polycarbonate Transwell plates as described previously |
67770 | Oil-contaminated soil of Biratnagar | Morang | Nepal | NPL | Asia |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Engineered | #Contamination | #Oil (Fuel) |
#Environmental | #Terrestrial | #Soil |
Sequence information
16S sequences
- @ref: 65475
- description: 16S rRNA gene sequence
- accession: MF405107
- database: nuccore
GC content
- @ref: 65475
- GC-content: 70.9
- method: high performance liquid chromatography (HPLC)
External links
@ref: 65475
culture collection no.: KEMB 9005-695, KACC 19390, JCM 32206
literature
- topic: Phylogeny
- Pubmed-ID: 29620494
- title: Rhodococcus olei sp. nov., with the ability to degrade petroleum oil, isolated from oil-contaminated soil.
- authors: Chaudhary DK, Kim J
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijsem.0.002750
- year: 2018
- mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Nepal, Nucleic Acid Hybridization, Peptidoglycan/chemistry, *Petroleum Pollution, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Rhodococcus/*classification/genetics/isolation & purification, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistry
- topic2: Transcriptome
Reference
@id | authors | title | doi/url | journal | pubmed |
---|---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | ||
65475 | Dhiraj Kumar Chaudhary, Jaisoo Kim | Rhodococcus olei sp. nov., with the ability to degrade petroleum oil, isolated from oil-contaminated soil | 10.1099/ijsem.0.002750 | IJSEM 68: 1749-1756 2018 | 29620494 |
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ |