Enterococcus wangshanyuanii (DSM 104047, CGMCC 1.15942, MN05)

Name: Enterococcus wangshanyuanii (DSM 104047, CGMCC 1.15942, MN05)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 158309

Type strain

Strain Designation MN05

Culture col. no. DSM 104047 CGMCC 1.15942 MN05

NCBI tax ID(s) 2005703

Special Collections DSMZ Literature strains

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Enterococcus wangshanyuanii MN05 is a facultative anaerobe, mesophilic, Gram-positive prokaryote that builds pairs and short chains or in irregular clusters and was isolated from faeces of yak .

Gram-positive ovoid-shaped facultative anaerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 401969

DSM 104047

@ref 20215

Last LPSN update 2025-12-16 10:07:59

Domain Bacillati

Phylum Bacillota

Class Bacilli

Order Lactobacillales

Family Enterococcaceae

Genus Enterococcus

Species Enterococcus wangshanyuanii

Full scientific name Enterococcus wangshanyuanii Jin et al. 2017

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
65222	positive	ovoid-shaped
65222		coccus-shaped

Colony morphology

@ref	Type of hemolysis	Hemolysis ability	Colony size	Colony color	Incubation period	Medium used
65222	alpha	1	1 mm	grey	1 day	Columbia agar

Multicellular morphology

@ref	Forms multicellular complex	Complex name
65222		pairs and short chains or in irregular clusters

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
64972	COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)		Medium recipe at MediaDive	Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
65222	Columbia agar			containing 5 % defibrinated sheep blood

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
64972	positive	growth	37	mesophilic
65222	positive	growth	22	psychrophilic
65222	positive	growth	35	mesophilic
65222	positive	growth	37	mesophilic
65222	positive	growth	45	thermophilic
65222	negative	growth	10

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
65222	facultative anaerobe
125439	obligate aerobe	93.1

Spore formation

65222

Spore formationno

Halophily

@ref	Salt	Growth	Tested relation	Concentration
65222	NaCl	positive	growth	6.5 %

Observation

65222

ObservationReacts with Lancefield group D antisera

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
65222		2-oxogluconate	-	builds acid from
65222	58143 ChEBI	5-dehydro-D-gluconate	-	builds acid from
65222	27613 ChEBI	amygdalin	+	builds acid from
65222	18305 ChEBI	arbutin	+	builds acid from
65222	29016 ChEBI	arginine	-	hydrolysis
65222	17057 ChEBI	cellobiose	+	builds acid from
65222	17108 ChEBI	D-arabinose	-	builds acid from
65222	18333 ChEBI	D-arabitol	-	builds acid from
65222	15824 ChEBI	D-fructose	+	builds acid from
65222	28847 ChEBI	D-fucose	-	builds acid from
65222	12936 ChEBI	D-galactose	+	builds acid from
65222	17634 ChEBI	D-glucose	+	builds acid from
65222	16024 ChEBI	D-mannose	+	builds acid from
65222	16443 ChEBI	D-tagatose	-	builds acid from
65222	65327 ChEBI	D-xylose	-	builds acid from
65222	65327 ChEBI	D-xylose	+	builds acid from
65222	17113 ChEBI	erythritol	-	builds acid from
65222	4853 ChEBI	esculin	+	builds acid from
65222	4853 ChEBI	esculin	+	hydrolysis
65222	16813 ChEBI	galactitol	-	builds acid from
65222	28066 ChEBI	gentiobiose	+	builds acid from
65222	24265 ChEBI	gluconate	+	builds acid from
65222	17234 ChEBI	glucose	-	builds gas from
65222	28087 ChEBI	glycogen	-	builds acid from
65222	606565 ChEBI	hippurate	+	hydrolysis
65222	15443 ChEBI	inulin	-	builds acid from
65222	30849 ChEBI	L-arabinose	-	builds acid from
65222	18403 ChEBI	L-arabitol	-	builds acid from
65222	18287 ChEBI	L-fucose	-	builds acid from
65222	62345 ChEBI	L-rhamnose	-	builds acid from
65222	17266 ChEBI	L-sorbose	-	builds acid from
65222	65328 ChEBI	L-xylose	-	builds acid from
65222	17716 ChEBI	lactose	+	builds acid from
65222	17306 ChEBI	maltose	+	builds acid from
65222	29864 ChEBI	mannitol	-	builds acid from
65222	6731 ChEBI	melezitose	-	builds acid from
65222	28053 ChEBI	melibiose	-	builds acid from
65222	320061 ChEBI	methyl alpha-D-glucopyranoside	-	builds acid from
65222	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from
65222	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from
65222	17268 ChEBI	myo-inositol	-	builds acid from
65222	506227 ChEBI	N-acetylglucosamine	-	builds acid from
65222	16634 ChEBI	raffinose	-	builds acid from
65222	15963 ChEBI	ribitol	-	builds acid from
65222	33942 ChEBI	ribose	+	builds acid from
65222	17814 ChEBI	salicin	+	builds acid from
65222	30911 ChEBI	sorbitol	-	builds acid from
65222	28017 ChEBI	starch	+	builds acid from
65222	17992 ChEBI	sucrose	-	builds acid from
65222	27082 ChEBI	trehalose	+	builds acid from
65222	32528 ChEBI	turanose	-	builds acid from
65222	17151 ChEBI	xylitol	-	builds acid from

Metabolite production

@ref	Chebi-ID	Metabolite	Production
65222	15688 ChEBI	acetoin

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test
65222	15688 ChEBI	acetoin	+

Enzymes

@ref	Value	Activity	Ec
65222	alkaline phosphatase	+	3.1.3.1
65222	alpha-galactosidase	-	3.2.1.22
65222	beta-galactosidase	-	3.2.1.23
65222	beta-glucuronidase	-	3.2.1.31
65222	catalase	-	1.11.1.6
65222	leucine arylamidase	+	3.4.11.1
65222	pyrrolidonyl arylamidase	+	3.4.19.3

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Mammals	#Bovinae (Cow, Cattle)
#Host Body Product	#Gastrointestinal tract	#Feces (Stool)

Isolation

@ref	Sample type	Sampling date	Geographic location	Country	Country ISO 3 Code	Continent	Enrichment culture	Enrichment culture duration	Enrichment culture temperature	Isolation procedure	Latitude	Longitude
64972	faeces of yak (Bos grunniens)		Qinghai province, Yushu tibetan autonomous prefecture (33° 48', 96° 51')	China	CHN	Asia					33.8	96.85 33.8/96.85
65222	faecal sample	2012	from faeces of yaks (Bos grunniens) in the Qinghai-Tibetan Plateau	China	CHN	Asia	KF streptococcus agar plates	2 days	37	Different colonies emerging on plates were re-streaked separately onto Columbia agar plates containing 5 % defibrinated sheep blood (bioM erieux), incubated in a 5 % CO2 atmosphere at 37°C for 24 h

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
64972	1	Risk group (German classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM219764v1 assembly for Enterococcus wangshanyuanii MN05	complete	GCA_002197645	2005703.3	2765235978	2005703	95.83
66792	ASM1464425v1 assembly for Enterococcus wangshanyuanii CGMCC 1.15942	contig	GCA_014644255	2005703.8	8113202216	2005703	67.77

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
64972	Enterococcus wangshanyuanii strain MN05 16S ribosomal RNA gene, partial sequence	MF326566	1518		2005703

GC content

@ref	GC-content (mol%)	Method
64972	37.5	sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Enterococcus wangshanyuanii CGMCC 1.15942
NCBI: GCA_014644255

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	71.80	no
125439	motility	BacteriaNetⓘ	yes	68.70	no
125439	gram_stain	BacteriaNetⓘ	variable	75.70	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	93.10	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Enterococcus wangshanyuanii MN05
NCBI: GCA_002197645.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	82.93	yes
125438	anaerobic	anaerobicⓘ	no	81.45	no
125438	aerobic	aerobicⓘ	no	95.65	no
125438	spore-forming	spore-formingⓘ	no	69.46	no
125438	thermophilic	thermophileⓘ	no	92.02	yes
125438	flagellated	motile2+ⓘ	no	88.50	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	A systematically biosynthetic investigation of lactic acid bacteria reveals diverse antagonistic bacteriocins that potentially shape the human microbiome.	Zhang D, Zhang J, Kalimuthu S, Liu J, Song ZM, He BB, Cai P, Zhong Z, Feng C, Neelakantan P, Li YX.	Microbiome	10.1186/s40168-023-01540-y	2023
Phylogeny	Enterococcus wangshanyuanii sp. nov., isolated from faeces of yaks (Bos grunniens).	Jin D, Yang J, Lu S, Lai XH, Xiong Y, Xu J	Int J Syst Evol Microbiol	10.1099/ijsem.0.002447	2017

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#64972	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 104047
#65222	Dong Jin, Jing Yang, Shan Lu, Xin-He Lai, YanWen Xiong, Jianguo Xu: Enterococcus wangshanyuanii sp. nov., isolated from faeces of yaks (Bos grunniens). IJSEM 67: 5216 - 5221 2017 ( DOI 10.1099/ijsem.0.002447 , PubMed 29072561 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Enterococcus wangshanyuanii (DSM 104047, CGMCC 1.15942, MN05)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Observation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_002197645

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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