Name: Enterococcus wangshanyuanii MN05
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 158309

Type strain:

Species: Enterococcus wangshanyuanii

Strain Designation: MN05

Culture col. no.: DSM 104047, CGMCC 1.15942

Strain history: <- D. Jin, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, P.R. China; MN05 <- Dong Jin

NCBI tax ID(s): 2005703 (species)

SI-ID 401969

DSM 104047

Special Collections

Section

Enterococcus wangshanyuanii MN05 is a facultative anaerobe, Gram-positive, ovoid-shaped bacterium that builds pairs and short chains or in irregular clusters and was isolated from faeces of yak .

Gram-positive
ovoid-shaped
facultative anaerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Lactobacillales*
	Family	*Enterococcaceae*
	Genus	*Enterococcus*
	Species	**Enterococcus wangshanyuanii**
	Full Scientific Name (LPSN)	Enterococcus wangshanyuanii Jin et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #65222]	Gram stain	positive

[Ref.: #65222]	Cell shape	ovoid-shaped
[Ref.: #65222]	Cell shape	coccus-shaped

[Ref.: #65222]	Motility	no

[Ref.: #65222]	Type of hemolysis	alpha
[Ref.: #65222]	Hemolysis ability	1
[Ref.: #65222]	Colony size	1 mm
[Ref.: #65222]	Colony color	grey
[Ref.: #65222]	Incubation period	1 day
[Ref.: #65222]	Cultivation medium used	Columbia agar

[Ref.: #65222]	Multicellular complex forming ability	yes
[Ref.: #65222]	Multicellular complex name	pairs and short chains or in irregular clusters

Information on culture and growth conditions Culture and growth conditions

[Ref.: #64972]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #64972]

Culture medium growth

[Ref.: #64972]

Culture medium link

Medium recipe at MediaDive

[Ref.: #64972]

Culture medium composition

expand / minimize

[Ref.: #65222]

Culture medium

Columbia agar

[Ref.: #65222]

Culture medium growth

[Ref.: #65222]

Culture medium composition

expand / minimize

	Kind of temperature		Temperature
[Ref.: #64972]		growth	37 °C
[Ref.: #65222]		growth	22 °C
[Ref.: #65222]		growth	35 °C
[Ref.: #65222]		growth	37 °C
[Ref.: #65222]		growth	45 °C
[Ref.: #65222]	no	growth	10 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #65222]

Oxygen tolerance

facultative anaerobe

[Ref.: #65222]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %93

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #65222]		NaCl		growth	6.5	%

[Ref.: #65222]

Observation

Reacts with Lancefield group D antisera

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #65222]	2-oxogluconate	-	builds acid from
[Ref.: #65222]	5-dehydro-D-gluconate ChEBI	-	builds acid from
[Ref.: #65222]	amygdalin ChEBI	+	builds acid from
[Ref.: #65222]	arbutin ChEBI	+	builds acid from
[Ref.: #65222]	arginine ChEBI	-	hydrolysis
[Ref.: #65222]	cellobiose ChEBI	+	builds acid from
[Ref.: #65222]	D-arabinose ChEBI	-	builds acid from
[Ref.: #65222]	D-arabitol ChEBI	-	builds acid from
[Ref.: #65222]	D-fructose ChEBI	+	builds acid from
[Ref.: #65222]	D-fucose ChEBI	-	builds acid from
[Ref.: #65222]	D-galactose ChEBI	+	builds acid from
[Ref.: #65222]	D-glucose ChEBI	+	builds acid from
[Ref.: #65222]	D-mannose ChEBI	+	builds acid from
[Ref.: #65222]	D-tagatose ChEBI	-	builds acid from
[Ref.: #65222]	D-xylose ChEBI	-	builds acid from
[Ref.: #65222]	D-xylose ChEBI	+	builds acid from
[Ref.: #65222]	erythritol ChEBI	-	builds acid from
[Ref.: #65222]	esculin ChEBI	+	builds acid from
[Ref.: #65222]	esculin ChEBI	+	hydrolysis
[Ref.: #65222]	galactitol ChEBI	-	builds acid from
[Ref.: #65222]	gentiobiose ChEBI	+	builds acid from
[Ref.: #65222]	gluconate ChEBI	+	builds acid from
[Ref.: #65222]	glucose ChEBI	-	builds gas from
[Ref.: #65222]	glycogen ChEBI	-	builds acid from
[Ref.: #65222]	hippurate ChEBI	+	hydrolysis
[Ref.: #65222]	inulin ChEBI	-	builds acid from
[Ref.: #65222]	L-arabinose ChEBI	-	builds acid from
[Ref.: #65222]	L-arabitol ChEBI	-	builds acid from
[Ref.: #65222]	L-fucose ChEBI	-	builds acid from
[Ref.: #65222]	L-rhamnose ChEBI	-	builds acid from
[Ref.: #65222]	L-sorbose ChEBI	-	builds acid from
[Ref.: #65222]	L-xylose ChEBI	-	builds acid from
[Ref.: #65222]	lactose ChEBI	+	builds acid from
[Ref.: #65222]	maltose ChEBI	+	builds acid from
[Ref.: #65222]	mannitol ChEBI	-	builds acid from
[Ref.: #65222]	melezitose ChEBI	-	builds acid from
[Ref.: #65222]	melibiose ChEBI	-	builds acid from
[Ref.: #65222]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from
[Ref.: #65222]	methyl alpha-D-mannoside ChEBI	-	builds acid from
[Ref.: #65222]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from
[Ref.: #65222]	myo-inositol ChEBI	-	builds acid from
[Ref.: #65222]	N-acetylglucosamine ChEBI	-	builds acid from
[Ref.: #65222]	raffinose ChEBI	-	builds acid from
[Ref.: #65222]	ribitol ChEBI	-	builds acid from
[Ref.: #65222]	ribose ChEBI	+	builds acid from
[Ref.: #65222]	salicin ChEBI	+	builds acid from
[Ref.: #65222]	sorbitol ChEBI	-	builds acid from
[Ref.: #65222]	starch ChEBI	+	builds acid from
[Ref.: #65222]	sucrose ChEBI	-	builds acid from
[Ref.: #65222]	trehalose ChEBI	+	builds acid from
[Ref.: #65222]	turanose ChEBI	-	builds acid from
[Ref.: #65222]	xylitol ChEBI	-	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #65222]		acetoin ChEBI	yes

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #65222]		acetoin ChEBI	+

	Enzyme	Enzyme activity	EC number
[Ref.: #65222]	alkaline phosphatase	+	3.1.3.1
[Ref.: #65222]	alpha-galactosidase	-	3.2.1.22
[Ref.: #65222]	beta-galactosidase	-	3.2.1.23
[Ref.: #65222]	beta-glucuronidase	-	3.2.1.31
[Ref.: #65222]	catalase	-	1.11.1.6
[Ref.: #65222]	leucine arylamidase	+	3.4.11.1
[Ref.: #65222]	pyrrolidonyl arylamidase	+	3.4.19.3

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #64972]	Sample type/isolated from	faeces of yak (Bos grunniens)
[Ref.: #64972]	Geographic location (country and/or sea, region)	Qinghai province, Yushu tibetan autonomous prefecture (33° 48', 96° 51')
[Ref.: #64972]	Country	China
[Ref.: #64972]	Country ISO 3 Code	CHN
[Ref.: #64972]	Continent	Asia
[Ref.: #64972]	Geographic location	33.8000°/96.8500°

[Ref.: #65222]	Sample type/isolated from	faecal sample
[Ref.: #65222]	Sampling date	2012
[Ref.: #65222]	Geographic location (country and/or sea, region)	from faeces of yaks (Bos grunniens) in the Qinghai-Tibetan Plateau
[Ref.: #65222]	Country	China
[Ref.: #65222]	Country ISO 3 Code	CHN
[Ref.: #65222]	Continent	Asia
[Ref.: #65222]	Enrichment culture	KF streptococcus agar plates
[Ref.: #65222]	Enrichment culture duration	2 days
[Ref.: #65222]	Enrichment culture temperature	37 ̊C
[Ref.: #65222]	Isolation/enrichment procedure	Different colonies emerging on plates were re-streaked separately onto Columbia agar plates containing 5 % defibrinated sheep blood (bioM erieux), incubated in a 5 % CO2 atmosphere at 37°C for 24 h
* marker position based on {}

Isolation sources categories

#Host	#Mammals	#Bovinae (Cow, Cattle)
#Host Body Product	#Gastrointestinal tract	#Feces (Stool)

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #64972]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #64972]	Enterococcus wangshanyuanii strain MN05 16S ribosomal RNA gene, partial sequence	MF326566	1518	GenBank ENA	2005703 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Enterococcus wangshanyuanii MN05	GCA_002197645	complete	GenBank ENA	2005703 tax ID	*
[Ref.: #66792]	Enterococcus wangshanyuanii CGMCC 1.15942	GCA_014644255	contig	GenBank ENA	2005703 tax ID	*
[Ref.: #66792]	Enterococcus wangshanyuanii strain CGMCC 1.15942	2005703.8	wgs	PATRIC	2005703 tax ID	*

[Ref.: #64972]

GC-content

37.5 mol%

sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Enterococcus wangshanyuanii MN05 NCBI: GCA_002197645.1
[Ref.: #69481]	spore-forming	spore-formingⓘ	no	93	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Enterococcus wangshanyuanii MN05 NCBI: GCA_002197645.1
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	82.931	yes
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	81.447	no
[Ref.: #69480]	aerobic	aerobicⓘ	no	95.652	no
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	69.457	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	92.017	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	no	88.5	no

Availability in culture collections External links

[Ref.: #64972]

Culture collection no.

DSM 104047, CGMCC 1.15942

[Ref.: #111123]

SI-ID 401969

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Enterococcus wangshanyuanii sp. nov., isolated from faeces of yaks (Bos grunniens).	Jin D, Yang J, Lu S, Lai XH, Xiong Y, Xu J	Int J Syst Evol Microbiol	10.1099/ijsem.0.002447	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#64972

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 104047

#65222

Dong Jin, Jing Yang, Shan Lu, Xin-He Lai, YanWen Xiong, Jianguo Xu: Enterococcus wangshanyuanii sp. nov., isolated from faeces of yaks (Bos grunniens). IJSEM 67: 5216 - 5221 2017 ( DOI 10.1099/ijsem.0.002447 , PubMed 29072561 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#111123

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID401969.1 )

* These data were automatically processed and therefore are not curated

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