Strain identifier

BacDive ID: 158309

Type strain: Yes

Species: Enterococcus wangshanyuanii

Strain Designation: MN05

Strain history: <- D. Jin, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, P.R. China; MN05 <- Dong Jin

NCBI tax ID(s): 2005703 (species)

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General

@ref: 64972

BacDive-ID: 158309

DSM-Number: 104047

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-positive, ovoid-shaped

description: Enterococcus wangshanyuanii MN05 is a facultative anaerobe, mesophilic, Gram-positive bacterium that builds pairs and short chains or in irregular clusters and was isolated from faeces of yak .

NCBI tax id

  • NCBI tax id: 2005703
  • Matching level: species

strain history

  • @ref: 64972
  • history: <- D. Jin, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, P.R. China; MN05 <- Dong Jin

doi: 10.13145/bacdive158309.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Enterococcaceae
  • genus: Enterococcus
  • species: Enterococcus wangshanyuanii
  • full scientific name: Enterococcus wangshanyuanii Jin et al. 2017

@ref: 64972

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Enterococcaceae

genus: Enterococcus

species: Enterococcus wangshanyuanii

full scientific name: Enterococcus wangshanyuanii Jin et al. 2017

strain designation: MN05

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotilityconfidence
65222positiveovoid-shapedno
65222coccus-shaped
69480no91.526
69480positive100

colony morphology

  • @ref: 65222
  • type of hemolysis: alpha
  • hemolysis ability: 1
  • colony size: 1 mm
  • colony color: grey
  • incubation period: 1 day
  • medium used: Columbia agar

multicellular morphology

  • @ref: 65222
  • forms multicellular complex: yes
  • complex name: pairs and short chains or in irregular clusters

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
64972COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
65222Columbia agaryescontaining 5 % defibrinated sheep blood

culture temp

@refgrowthtypetemperaturerange
64972positivegrowth37mesophilic
65222positivegrowth22psychrophilic
65222positivegrowth35mesophilic
65222positivegrowth37mesophilic
65222positivegrowth45thermophilic
65222nogrowth10psychrophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 65222
  • oxygen tolerance: facultative anaerobe

spore formation

@refspore formationconfidence
65222no
69480no99.83
69481no93

halophily

  • @ref: 65222
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 6.5 %

observation

  • @ref: 65222
  • observation: Reacts with Lancefield group D antisera

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
652222-oxogluconate-builds acid from
652225-dehydro-D-gluconate-builds acid from58143
65222amygdalin+builds acid from27613
65222arbutin+builds acid from18305
65222arginine-hydrolysis29016
65222cellobiose+builds acid from17057
65222ribitol-builds acid from15963
65222D-arabinose-builds acid from17108
65222D-arabitol-builds acid from18333
65222D-fructose+builds acid from15824
65222D-fucose-builds acid from28847
65222D-galactose+builds acid from12936
65222D-glucose+builds acid from17634
65222D-mannose+builds acid from16024
65222D-tagatose-builds acid from16443
65222D-xylose-builds acid from65327
65222D-xylose+builds acid from65327
65222galactitol-builds acid from16813
65222erythritol-builds acid from17113
65222esculin+builds acid from4853
65222esculin+hydrolysis4853
65222gentiobiose+builds acid from28066
65222gluconate+builds acid from24265
65222glucose-builds gas from17234
65222glycogen-builds acid from28087
65222hippurate+hydrolysis606565
65222inulin-builds acid from15443
65222L-arabinose-builds acid from30849
65222L-arabitol-builds acid from18403
65222L-fucose-builds acid from18287
65222L-rhamnose-builds acid from62345
65222L-sorbose-builds acid from17266
65222L-xylose-builds acid from65328
65222lactose+builds acid from17716
65222maltose+builds acid from17306
65222mannitol-builds acid from29864
65222melezitose-builds acid from6731
65222melibiose-builds acid from28053
65222methyl alpha-D-glucopyranoside-builds acid from320061
65222methyl alpha-D-mannoside-builds acid from43943
65222methyl beta-D-xylopyranoside-builds acid from74863
65222myo-inositol-builds acid from17268
65222N-acetylglucosamine-builds acid from506227
65222raffinose-builds acid from16634
65222ribose+builds acid from33942
65222salicin+builds acid from17814
65222sorbitol-builds acid from30911
65222starch+builds acid from28017
65222sucrose-builds acid from17992
65222trehalose+builds acid from27082
65222turanose-builds acid from32528
65222xylitol-builds acid from17151

metabolite production

  • @ref: 65222
  • Chebi-ID: 15688
  • metabolite: acetoin
  • production: yes

metabolite tests

  • @ref: 65222
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: +

enzymes

@refvalueactivityec
65222catalase-1.11.1.6
65222pyrrolidonyl arylamidase+3.4.19.3
65222alkaline phosphatase+3.1.3.1
65222leucine arylamidase+3.4.11.1
65222alpha-galactosidase-3.2.1.22
65222beta-glucuronidase-3.2.1.31
65222beta-galactosidase-3.2.1.23

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudesampling dateenrichment cultureenrichment culture durationenrichment culture temperatureisolation procedure
64972faeces of yak (Bos grunniens)Qinghai province, Yushu tibetan autonomous prefecture (33° 48', 96° 51')ChinaCHNAsia33.896.85
65222faecal samplefrom faeces of yaks (Bos grunniens) in the Qinghai-Tibetan PlateauChinaCHNAsia2012KF streptococcus agar plates2 days37Different colonies emerging on plates were re-streaked separately onto Columbia agar plates containing 5 % defibrinated sheep blood (bioM erieux), incubated in a 5 % CO2 atmosphere at 37°C for 24 h

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Bovinae (Cow, Cattle)
#Host Body Product#Gastrointestinal tract#Feces (Stool)

Safety information

risk assessment

  • @ref: 64972
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 64972
  • description: Enterococcus wangshanyuanii strain MN05 16S ribosomal RNA gene, partial sequence
  • accession: MF326566
  • length: 1518
  • database: ena
  • NCBI tax ID: 2005703

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Enterococcus wangshanyuanii MN05GCA_002197645completencbi2005703
66792Enterococcus wangshanyuanii CGMCC 1.15942GCA_014644255contigncbi2005703
66792Enterococcus wangshanyuanii strain CGMCC 1.159422005703.8wgspatric2005703

GC content

  • @ref: 64972
  • GC-content: 37.5
  • method: sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno93no
flagellatedno98.198yes
gram-positiveyes91.334yes
anaerobicno92.084yes
aerobicno95.172yes
halophileyes76.23no
spore-formingno87.801yes
glucose-utilyes88.311no
thermophileno99.324no
motileno94.72yes
glucose-fermentyes83.673no

External links

@ref: 64972

culture collection no.: DSM 104047, CGMCC 1.15942

straininfo link

  • @ref: 111123
  • straininfo: 401969

literature

  • topic: Phylogeny
  • Pubmed-ID: 29072561
  • title: Enterococcus wangshanyuanii sp. nov., isolated from faeces of yaks (Bos grunniens).
  • authors: Jin D, Yang J, Lu S, Lai XH, Xiong Y, Xu J
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002447
  • year: 2017
  • mesh: Animals, Bacterial Typing Techniques, Base Composition, Cattle/*microbiology, China, DNA, Bacterial/genetics, Enterococcus/*classification/genetics/isolation & purification, Feces/*microbiology, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
64972Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-104047Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 104047)
65222Dong Jin, Jing Yang, Shan Lu, Xin-He Lai, YanWen Xiong, Jianguo XuEnterococcus wangshanyuanii sp. nov., isolated from faeces of yaks (Bos grunniens)10.1099/ijsem.0.002447IJSEM 67: 5216-5221 201729072561
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
111123Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID401969.1