Name: Desulfonatronum parangueonense PAR180
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 158308

Type strain:

Species: Desulfonatronum parangueonense

Strain Designation: PAR180

Culture col. no.: DSM 103602, JCM 31598

Strain history: A. Hirschler-Réa; Aix-Marseille Univ., France; PAR180.

NCBI tax ID(s): 2047730 (species)

SI-ID 401976

DSM 103602

Special Collections

Section

Desulfonatronum parangueonense PAR180 is an anaerobe, halotolerant, Gram-negative bacterium that was isolated from sediment of an alkaline crater lake located in a maar of phreatomagmatic origin.

Gram-negative
motile
vibrio-shaped
anaerobe
halotolerant
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Deltaproteobacteria*
	Order	*Desulfovibrionales*
	Family	*Desulfonatronaceae*
	Genus	*Desulfonatronum*
	Species	**Desulfonatronum parangueonense**
	Full Scientific Name (LPSN)	Desulfonatronum parangueonense Pérez Bernal et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #65221]	Gram stain	negative

[Ref.: #65221]	Cell length	1.2-2.3 µm

[Ref.: #65221]	Cell width	0.7-0.9 µm

[Ref.: #65221]	Cell shape	vibrio-shaped

[Ref.: #65221]	Motility	yes

[Ref.: #65221]	Flagellum arrangement	monotrichous, polar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #64971]

Culture medium

DESULFONATRONUM LACUSTRE MEDIUM (DSMZ Medium 813b)

[Ref.: #64971]

Culture medium growth

[Ref.: #64971]

Culture medium link

Medium recipe at MediaDive

[Ref.: #64971]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #64971]	growth	35 °C
[Ref.: #65221]	growth	15-40 °C
[Ref.: #65221]	optimum	35 °C
[Ref.: #67770]	growth	35 °C

	pH	Kind of pH		pH
[Ref.: #65221]			optimum	9
[Ref.: #65221]			growth	8.3-10.4

Information on physiology and metabolism Physiology and metabolism

[Ref.: #65221]

Oxygen tolerance

anaerobe

[Ref.: #65221]

Ability of spore formation

[Ref.: #65221]	Halophily / tolerance level	halotolerant
[Ref.: #65221]	Halophily / tolerance level	halotolerant

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #65221]		NaCl		growth	0-80	g/L
[Ref.: #65221]		NaCl		optimum	5	g/L

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #64971]	Sample type/isolated from	sediment of an alkaline crater lake located in a maar of phreatomagmatic origin
[Ref.: #64971]	Geographic location (country and/or sea, region)	state of Guanajuato, Vallee de Santiago, Rincón de Parangueo crater
[Ref.: #64971]	Country	Mexico
[Ref.: #64971]	Country ISO 3 Code	MEX
[Ref.: #64971]	Continent	North America
[Ref.: #64971]	Geographic location	20.4167°/-101.2000°

[Ref.: #65221]	Sample type/isolated from	sediment sample
[Ref.: #65221]	Sampling date	2013-09
[Ref.: #65221]	Geographic location (country and/or sea, region)	crater soda lake, Rincón de Parangueo
[Ref.: #65221]	Country	Mexico
[Ref.: #65221]	Country ISO 3 Code	MEX
[Ref.: #65221]	Continent	North America
[Ref.: #65221]	Geographic location	20.4166°/-101.2020°
[Ref.: #65221]	Enrichment culture	1 : 1 (v/v) proportion with filtered water of the sampling site and distilled water
[Ref.: #65221]	Enrichment culture composition	(per litre): Na 2 SO 4 , 4 g; Na 2 S 2 O 3 , 0.79 g; NH 4 Cl, 0.25 g; KH 2 PO 4 , 0.2 g; NaCl, 15 g; yeast extract, 0.1 g; HEPES, 10 mM; selenite tungstate solution [18], 1 ml; trace element SL12 solution [19], 1 ml; resazurin solution 0.1 % (w/v), 1 ml. The pH was adjusted to 9.
[Ref.: #65221]	Enrichment culture duration	3-4 weeks
[Ref.: #65221]	Enrichment culture temperature	30 ̊C
[Ref.: #65221]	Isolation/enrichment procedure	After sterilization, the culture medium was immediately flushed with nitrogen gas. Prior to use, the following components were added from anoxic sterile solutions: vitamin V7 solution [20], 1 ml; FeSO4-7H2O, 1 mM, to monitor sulfidogenic activity (removal of sulfide by production of FeS); and Na2S2O4 , 0.2 mM, as reductant. A mixture of four substrates, namely sodium acetate, sodium lactate, sodium pyruvate and glycerol (5 mM each), was added to serum bottles containing 50 ml of culture medium.

[Ref.: #67770]	Sample type/isolated from	Sediment collected at an alkaline crater lake in Guanajuato
[Ref.: #67770]	Country	Mexico
[Ref.: #67770]	Country ISO 3 Code	MEX
[Ref.: #67770]	Continent	North America
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Lake (large)
#Environmental	#Aquatic	#Sediment
#Condition	#Alkaline	-

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #64971]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #64971]	Desulfonatronum parangueonense strain PAR180 16S ribosomal RNA gene, partial sequence	KY041865	1518	GenBank ENA	2047730 tax ID

[Ref.: #64971]

GC-content

56.1 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #64971]

Culture collection no.

DSM 103602, JCM 31598

[Ref.: #111122]

SI-ID 401976

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Desulfonatronum parangueonense sp. nov., a sulfate-reducing bacterium isolated from sediment of an alkaline crater lake.	Perez Bernal MF, Souza Brito EM, Bartoli M, Aube J, Fardeau ML, Cuevas Rodriguez G, Ollivier B, Guyoneaud R, Hirschler-Rea A	Int J Syst Evol Microbiol	10.1099/ijsem.0.002399	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#64971

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 103602

#65221

Maria Fernanda Pérez Bernal, Elcia Margareth Souza Brito, Manon Bartoli, Johanne Aubé, Marie-Laure Fardeau, German Cuevas Rodriguez, Bernard Ollivier, Rémy Guyoneaud, Agnès Hirschler-Réa: Desulfonatronum parangueonense sp. nov., a sulfate-reducing bacterium isolated from sediment of an alkaline crater lake. IJSEM 67: 4999 - 5005 2017 ( DOI 10.1099/ijsem.0.002399 , PubMed 29039304 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#111122

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID401976.1 )

* These data were automatically processed and therefore are not curated

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