Streptomyces melanosporofaciens (DSM 40318, ATCC 25473, CBS 883.69)

Name: Streptomyces melanosporofaciens (DSM 40318, ATCC 25473, CBS 883.69)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 15399

Type strain

Strain Designation 1573

Culture col. no. DSM 40318 ATCC 25473 CBS 883.69 IFO 13061 ISP 5318 9 more

NCBI tax ID(s) 67327

Special Collections DSMZ JCM

History DSMZ <- E.B. Shirling, ISP <- B. Camerino, Farmitalia, Fi 1573 JCM <-- KCC S-0495 <-- T. Furumai <-- SAJ <-- ISP 5318 <-- Farmitalia FI 1573.

Links

Streptomyces melanosporofaciens 1573 is a bacterium that produces antibiotic compounds and was isolated from soil.

antibiotic compound production genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 389365

ATCC 25473,IFO 13061,DSM 40318,JCM 4495,CCRC 12064,NCIMB 12978,NRRL B-12234,VKM Ac-1864,CBS 883.69,NBRC 13061,BCRC 12064,BCC 7127,CGMCC 4.1742

@ref 20215

Last LPSN update 2026-05-29 08:28:32

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Kitasatosporales

Family Streptomycetaceae

Genus Streptomyces

Species Streptomyces melanosporofaciens

Full scientific name Streptomyces melanosporofaciens Arcamone et al. 1959 (Approved Lists 1980)

Morphology

Cell morphology

@ref	Motility	Confidence	Gram stain
125439		93.917
125439		97.757	positive

Multimedia

@ref:	9456
multimedia content:	DSM_40318.jpg
multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_40318.jpg
caption:	Medium 65 28°C
intellectual property rights:	© Leibniz-Institut DSMZ
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
9456	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)	Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
9456	STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252)	Medium recipe at MediaDive	Name: STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) Composition: Agar 20.0 g/l Starch 10.0 g/l (NH4)2SO4 2.0 g/l CaCO3 2.0 g/l K2HPO4 1.0 g/l MgSO4 x 7 H2O 1.0 g/l NaCl 1.0 g/l FeSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l ZnSO4 x 7 H2O 0.001 g/l Distilled water
9456	ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)	Medium recipe at MediaDive	Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
9456	positive	growth	28
67770	positive	growth	28

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
125438	aerobe	92.715
125439	obligate aerobe	96.474

Spore formation

@ref	Spore formation	Confidence
125438		92.761

Compound production

@ref	Compound
9456	elaiophylin(=azalomycin B)
9456	melanosporin
67770	Melanosporin

Metabolite production

@ref	Chebi-ID	Metabolite	Production
67770	77879 ChEBI	elaiophylin

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil

Isolation

@ref	Sample type	Country	Country ISO 3 Code	Continent
9456	soil	Italy	ITA	Europe

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
9456	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	IMG-taxon 2634166371 annotated assembly for Streptomyces melanosporofaciens DSM 40318	contig	GCA_900105695	67327.3	2634166371	67327	78.14

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Streptomyces melanosporofaciens strain CGMCC 4.1742 16S ribosomal RNA gene, partial sequence	HQ244452	1401	67327
20218	Streptomyces melanosporofaciens gene for 16S ribosomal RNA, partial sequence, strain: JCM 4495	D44187	121	67327
20218	Streptomyces melanosporofaciens gene for 16S rRNA, partial sequence, strain: NBRC 13061	AB184283	1475	67327
20218	Streptomyces melanosporofaciens 16S rRNA gene, strain NRRL B-12234T	AJ271887	1483	67327
20218	Streptomyces melanosporofaciens partial 16S rRNA gene, type strain NRRL B-12234T	AJ391837	1483	67327

GC content

@ref	GC-content (mol%)	Method
67770	71	thermal denaturation, midpoint method (Tm)
67770	71	genome sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: IMG-taxon 2634166371 annotated assembly for Streptomyces melanosporofaciens DSM 40318
BV-BRC: 67327.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	96.47	no
125439	gram_stain	BacteriaNetⓘ	positive	97.76	no
125439	spore_formation	BacteriaNetⓘ	yes	85.46	no
125439	motility	BacteriaNetⓘ	no	93.92	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Streptomyces melanosporofaciens strain DSM 40318
PATRIC: 67327.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	86.20	no
125438	anaerobic	anaerobicⓘ	no	97.33	no
125438	spore-forming	spore-formingⓘ	yes	92.76	no
125438	aerobic	aerobicⓘ	yes	92.72	no
125438	thermophilic	thermophileⓘ	no	97.00	no
125438	flagellated	motile2+ⓘ	no	88.50	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Endophytic Streptomyces sp. NEAU-ZSY13 from the leaf of Perilla frutescens, as a promising broad-spectrum biocontrol agent against soil-borne diseases.	Wang Z, Gao C, Yang J, Du R, Zeng F, Bing H, Xia B, Shen Y, Liu C.	Front Microbiol	10.3389/fmicb.2023.1243610	2023
Pathogenicity	Nigericin and Geldanamycin Are Phytotoxic Specialized Metabolites Produced by the Plant Pathogen Streptomyces sp. 11-1-2.	Diaz-Cruz GA, Liu J, Tahlan K, Bignell DRD.	Microbiol Spectr	10.1128/spectrum.02314-21	2022
Metabolism	Aminoacyl sulfonamide assembly in SB-203208 biosynthesis.	Hu Z, Awakawa T, Ma Z, Abe I.	Nat Commun	10.1038/s41467-018-08093-x	2019
Genetics	Biocatalytic diversification of abietic acid in Streptomyces.	McCadden CA, Alsup TA, Ghiviriga I, Rudolf JD.	J Ind Microbiol Biotechnol	10.1093/jimb/kuaf003	2024
Genetics	Genome mining reveals a highly oxidized bacterial perhydrophenanthrene meroterpenoid smelanic acid.	Yang Z, Pan X, Deng L, Wang Z, Li FR, Lin X, Xu HM, Dong LB.	Org Biomol Chem	10.1039/d5ob01195g	2025
	Efficacy of Streptomyces melanosporofaciens strain X216 at controlling clubroot disease on oilseed rape.	Ding L, Zhou H, Liang HD, Tan L, Zhao H, Chen XJ, Ren ZH.	Front Microbiol	10.3389/fmicb.2023.1249813	2023
	Identification of potent anti-Candida metabolites produced by the soft coral associated Streptomyces sp. HC14 using chemoinformatics.	Abdella B, Abdella M, ElSharif HA, ElAhwany AMD, El-Sersy NA, Ghozlan HA, Sabry SA.	Sci Rep	10.1038/s41598-023-39568-7	2023
	Bacillus atrophaeus DX-9 biocontrol against potato common scab involves significant changes in the soil microbiome and metabolome.	Cao J, Ma Y, Fu J, Wang Z, Zhao Y, Zhong N, Zhao P.	aBIOTECH	10.1007/s42994-025-00199-3	2025
	Understanding the role of active site residues in CotB2 catalysis using a cluster model.	Raz K, Driller R, Bruck T, Loll B, Major DT.	Beilstein J Org Chem	10.3762/bjoc.16.7	2020
Enzymology	The first structure of a bacterial diterpene cyclase: CotB2.	Janke R, Gorner C, Hirte M, Bruck T, Loll B.	Acta Crystallogr D Biol Crystallogr	10.1107/s1399004714005513	2014
	Microbial dynamics, chemical profile, and bioactive potential of diverse Egyptian marine environments from archaeological wood to soda lake.	Hegazy GE, Moawad MN, Othman SS, Soliman NA, Abeer E A, Oraby H, Abdel-Fattah YR.	Sci Rep	10.1038/s41598-024-70411-9	2024
	Genome shuffling enhances biocontrol abilities of Streptomyces strains against two potato pathogens.	Clermont N, Lerat S, Beaulieu C.	J Appl Microbiol	10.1111/j.1365-2672.2011.05078.x	2011
Pathogenicity	Antiviral activity of methylelaiophylin, an alpha-glucosidase inhibitor.	Lee D, Woo JK, Kim D, Kim M, Cho SK, Kim JH, Park SP, Lee HY, Riu KZ, Lee DS.	J Microbiol Biotechnol	10.4014/jmb.1011.11002	2011
	Enzyme-Catalysed Formation of Hydrocarbon Scaffolds from Geranylgeranyl Diphosphate Analogs with Shifted Double Bonds.	Li H, Goldfuss B, Dickschat JS.	Chemistry	10.1002/chem.202500712	2025
Biotechnology	Current understanding and biotechnological application of the bacterial diterpene synthase CotB2.	Driller R, Garbe D, Mehlmer N, Fuchs M, Raz K, Major DT, Bruck T, Loll B.	Beilstein J Org Chem	10.3762/bjoc.15.228	2019
Phylogeny	The Streptomyces violaceusniger clade: a home for Streptomycetes with rugose ornamented spores.	Goodfellow M, Kumar Y, Labeda DP, Sembiring L.	Antonie Van Leeuwenhoek	10.1007/s10482-007-9146-6	2007
Enzymology	Microscopic examination of chitosan-polyphosphate beads with entrapped spores of the biocontrol agent, Streptomyces melanosporofaciens EF-76.	Jobin G, Grondin G, Couture G, Beaulieu C.	Microsc Microanal	10.1017/s1431927605050142	2005
	Effects of Bacillus cereus strain Jdm1 on Meloidogyne incognita and the bacterial community in tomato rhizosphere soil.	Xiao L, Wan JW, Yao JH, Feng H, Wei LH.	3 Biotech	10.1007/s13205-018-1348-2	2018
Genetics	Genome Characteristics Reveal the Biocontrol Potential of Actinobacteria Isolated From Sugarcane Rhizosphere.	Wang Z, Solanki MK, Yu ZX, Anas M, Dong DF, Xing YX, Malviya MK, Pang F, Li YR.	Front Microbiol	10.3389/fmicb.2021.797889	2021
	Antagonistic properties of two recombinant strains of Streptomyces melanosporofaciens obtained by intraspecific protoplast fusion.	Agbessi S, Beausejour J, Dery C, Beaulieu C.	Appl Microbiol Biotechnol	10.1007/s00253-003-1256-0	2003
Metabolism	Biotechnological preparation of the elaiophylin.	Cikosova M, Blazsek M, Kubis M, Gajdosikova J, Borosova G.	Folia Microbiol (Praha)	10.1007/bf02931557	2004
	Streptomycete spores entrapped in chitosan beads as a novel biocontrol tool against common scab of potato.	Jobin G, Couture G, Goyer C, Brzezinski R, Beaulieu C.	Appl Microbiol Biotechnol	10.1007/s00253-004-1843-8	2005
	Major Biological Control Strategies for Plant Pathogens.	Pandit MA, Kumar J, Gulati S, Bhandari N, Mehta P, Katyal R, Rawat CD, Mishra V, Kaur J.	Pathogens	10.3390/pathogens11020273	2022
	Dibutyl phthalate, an alpha-glucosidase inhibitor from Streptomyces melanosporofaciens.	Lee DS.	J Biosci Bioeng	10.1016/s1389-1723(00)88832-5	2000
Genetics	Isolation and characterization of phthalates from Brevibacterium mcbrellneri that cause cytotoxicity and cell cycle arrest.	Rajamanikyam M, Vadlapudi V, Parvathaneni SP, Koude D, Sripadi P, Misra S, Amanchy R, Upadhyayula SM.	EXCLI J	10.17179/excli2017-145	2017
	Stereoselective chemo-enzymatic oxidation routes for (1R,3E,7E,11S,12S)-3,7,18-dolabellatriene.	Gorner C, Hirte M, Huber S, Schrepfer P, Bruck T.	Front Microbiol	10.3389/fmicb.2015.01115	2015
Pathogenicity	Cyclooctatin, a new inhibitor of lysophospholipase, produced by Streptomyces melanosporofaciens MI614-43F2. Taxonomy, production, isolation, physico-chemical properties and biological activities.	Aoyagi T, Aoyama T, Kojima F, Hattori S, Honma Y, Hamada M, Takeuchi T.	J Antibiot (Tokyo)	10.7164/antibiotics.45.1587	1992
Phylogeny	Identification of mycobacteria by matrix-assisted laser desorption ionization-time-of-flight mass spectrometry.	Pignone M, Greth KM, Cooper J, Emerson D, Tang J.	J Clin Microbiol	10.1128/jcm.01959-05	2006
Metabolism	Identification, characterization and molecular adaptation of class I redox systems for the production of hydroxylated diterpenoids.	Gorner C, Schrepfer P, Redai V, Wallrapp F, Loll B, Eisenreich W, Haslbeck M, Bruck T.	Microb Cell Fact	10.1186/s12934-016-0487-6	2016
Enzymology	Isolation and characterization of a rhizobacterial antagonist of root-knot nematodes.	Wei L, Shao Y, Wan J, Feng H, Zhu H, Huang H, Zhou Y.	PLoS One	10.1371/journal.pone.0085988	2014
	Recent highlights in biosynthesis research using stable isotopes.	Rinkel J, Dickschat JS.	Beilstein J Org Chem	10.3762/bjoc.11.271	2015
	Effect of biocontrol agent Pseudomonas fluorescens 2P24 on soil fungal community in cucumber rhizosphere using T-RFLP and DGGE.	Gao G, Yin D, Chen S, Xia F, Yang J, Li Q, Wang W.	PLoS One	10.1371/journal.pone.0031806	2012
	Microbial inoculants and their impact on soil microbial communities: a review.	Trabelsi D, Mhamdi R.	Biomed Res Int	10.1155/2013/863240	2013
	Identification of amino acid networks governing catalysis in the closed complex of class I terpene synthases.	Schrepfer P, Buettner A, Goerner C, Hertel M, van Rijn J, Wallrapp F, Eisenreich W, Sieber V, Kourist R, Bruck T.	Proc Natl Acad Sci U S A	10.1073/pnas.1519680113	2016
Genetics	Genome-Based Taxonomic Classification of the Phylum Actinobacteria.	Nouioui I, Carro L, Garcia-Lopez M, Meier-Kolthoff JP, Woyke T, Kyrpides NC, Pukall R, Klenk HP, Goodfellow M, Goker M.	Front Microbiol	10.3389/fmicb.2018.02007	2018
Metabolism	[Anionic carbohydrate-containing cell wall polymers of Streptomyces melanosporofaciens and related species].	Tul'skaia EM, Shashkov AS, Bueva OV, Evtushenko LI	Mikrobiologiia		2007
Genetics	Genome-based classification of the Streptomyces violaceusniger clade and description of Streptomyces sabulosicollis sp. nov. from an Indonesian sand dune.	Kusuma AB, Nouioui I, Goodfellow M.	Antonie Van Leeuwenhoek	10.1007/s10482-021-01564-0	2021

References

#9456	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 40318
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Streptomyces melanosporofaciens (DSM 40318, ATCC 25473, CBS 883.69)

Name and taxonomic classification

Morphology

Cell morphology

Multimedia

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Compound production

Metabolite production

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_900105695

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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