Name: Staphylococcus saprophyticus KV 12
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 14669

Type strain:

Species: Staphylococcus saprophyticus

Strain Designation: KV 12, KV 12T

Culture col. no.: DSM 18669, CCM 4410, CCUG 36975, CCUG 38042, CIP 105260

Strain history: CIP <- 1997, J. Freney, Herriot Hosp., Lyon, France <- V. Hajek, Palacky Univ., Olomouc, Czech Republic: strain KV 12T

NCBI tax ID(s): 29385 (species)

SI-ID 57291

CCUG 36975, CCM 4410, CCUG 38042, CIP 105260, DSM 18669

Special Collections

Other strains from Staphylococcus saprophyticus

Section

Staphylococcus saprophyticus KV 12 is a facultative anaerobe, Gram-positive, coccus-shaped bacterium that was isolated from bovine nostril.

coccus-shaped
Gram-positive
facultative anaerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Staphylococcaceae*
	Genus	*Staphylococcus*
	Species	**Staphylococcus saprophyticus**
	Full Scientific Name (LPSN)	Staphylococcus saprophyticus (Fairbrother 1940) Shaw et al. 1951 (Approved Lists 1980)

	Synonym	Staphylococcus saprophyticus subsp. bovis
	Synonym	"Staphylococcus* saprophyticus"*

Information on morphological and physiological properties Morphology

[Ref.: #122487]	Gram stain	positive

[Ref.: #122487]	Cell shape	coccus-shaped

[Ref.: #122487]	Motility	no

[Ref.: #7650]	Type of hemolysis	gamma
[Ref.: #7650]	Incubation period	1-2 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #7650]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #7650]

Culture medium growth

[Ref.: #7650]

Culture medium link

Medium recipe at MediaDive

[Ref.: #7650]

Culture medium composition

expand / minimize

[Ref.: #7650]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

[Ref.: #7650]

Culture medium growth

[Ref.: #7650]

Culture medium link

Medium recipe at MediaDive

[Ref.: #7650]

Culture medium composition

expand / minimize

[Ref.: #32746]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #32746]

Culture medium growth

[Ref.: #32746]

Culture medium composition

expand / minimize

[Ref.: #122487]

Culture medium

CIP Medium 3

[Ref.: #122487]

Culture medium growth

[Ref.: #122487]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #122487]

Culture medium

CIP Medium 72

[Ref.: #122487]

Culture medium growth

[Ref.: #122487]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #7650]		growth	37 °C
[Ref.: #32746]		growth	30 °C
[Ref.: #122487]		growth	22-41 °C
[Ref.: #122487]	no	growth	10 °C
[Ref.: #122487]	no	growth	45 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #122487]

Oxygen tolerance

facultative anaerobe

[Ref.: #69481]

Ability of spore formation

no Confidence in %99

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68375]	arabinose ChEBI	-	fermentation	* from API ID32STA
[Ref.: #68375]	arginine ChEBI	-	hydrolysis	* from API ID32STA
[Ref.: #68375]	cellobiose ChEBI	-	fermentation	* from API ID32STA
[Ref.: #68375]	D-fructose ChEBI	+	fermentation	* from API ID32STA
[Ref.: #68375]	D-glucose ChEBI	+	fermentation	* from API ID32STA
[Ref.: #68375]	D-mannitol ChEBI	+	fermentation	* from API ID32STA
[Ref.: #68375]	D-mannose ChEBI	-	fermentation	* from API ID32STA
[Ref.: #68375]	D-ribose ChEBI	-	fermentation	* from API ID32STA
[Ref.: #68375]	esculin ChEBI	-	hydrolysis	* from API ID32STA
[Ref.: #122487]	hippurate ChEBI	+	hydrolysis
[Ref.: #68375]	lactose ChEBI	-	fermentation	* from API ID32STA
[Ref.: #68375]	maltose ChEBI	+	fermentation	* from API ID32STA
[Ref.: #68375]	N-acetylglucosamine ChEBI	+	fermentation	* from API ID32STA
[Ref.: #122487]	nitrate ChEBI	+	reduction
[Ref.: #68375]	nitrate ChEBI	+	reduction	* from API ID32STA
[Ref.: #122487]	nitrite ChEBI	-	reduction
[Ref.: #68375]	ornithine ChEBI	-	degradation	* from API ID32STA
[Ref.: #68375]	raffinose ChEBI	-	fermentation	* from API ID32STA
[Ref.: #68375]	sucrose ChEBI	+	fermentation	* from API ID32STA
[Ref.: #68375]	trehalose ChEBI	+	fermentation	* from API ID32STA
[Ref.: #68375]	turanose ChEBI	+	fermentation	* from API ID32STA
[Ref.: #68375]	urea ChEBI	+	hydrolysis	* from API ID32STA
	Only first 10 entries are displayed. Click here to see all.

	Antibiotica	Metabolite	Antibiotic resistance	Concentration
[Ref.: #68375]		novobiocin ChEBI	yes	1.8	µg	* from API ID32STA

	Metabolite production	Metabolite	Production
[Ref.: #68375]		acetoin ChEBI	yes	* from API ID32STA

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #122487]		acetoin ChEBI	-
[Ref.: #68375]		acetoin ChEBI	+	* from API ID32STA

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #122487]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #122487]	amylase	-
[Ref.: #68375]	arginine dihydrolase	-	3.5.3.6	* from API ID32STA
[Ref.: #122487]	beta-galactosidase	+	3.2.1.23
[Ref.: #68375]	beta-galactosidase	+	3.2.1.23	* from API ID32STA
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68375]	beta-glucosidase	-	3.2.1.21	* from API ID32STA, API zym
[Ref.: #68375]	beta-glucuronidase	-	3.2.1.31	* from API ID32STA, API zym
[Ref.: #122487]	caseinase	-	3.4.21.50
[Ref.: #7650]	catalase	+	1.11.1.6
[Ref.: #122487]	catalase	+	1.11.1.6
[Ref.: #122487]	coagulase	-
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #7650]	cytochrome-c oxidase	-	1.9.3.1
[Ref.: #122487]	DNase	-
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #122487]	gamma-glutamyltransferase	-	2.3.2.2
[Ref.: #122487]	gelatinase	+
[Ref.: #68375]	L-arginine arylamidase	-		* from API ID32STA
[Ref.: #122487]	lecithinase	-
[Ref.: #68382]	leucine arylamidase	-	3.4.11.1	* from API zym
[Ref.: #122487]	lipase	-
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #122487]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #122487]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #68375]	ornithine decarboxylase	-	4.1.1.17	* from API ID32STA
[Ref.: #122487]	oxidase	-
[Ref.: #122487]	phenylalanine ammonia-lyase	-	4.3.1.24
[Ref.: #68375]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API ID32STA
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #122487]	tween esterase	-
[Ref.: #68375]	urease	+	3.5.1.5	* from API ID32STA
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #122487]	1081	not determinedn.d.	+	+	+	+	-	-	-	+	-	+	+	+	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	+	-	-	+	-	+	+	+	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	+	-	-	-	+	+	-	-	-	-	-	-	-

API® ID32STA:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Urease/urea hydrolysisURE	Arginine dihydrolaseADH (Arg)	Ornithine decarboxylaseODC	Esculin hydrolysisESC	Fermentation of D-glucoseGLU	Fermentation of D-fructoseFRU	Fermentation of D-mannoseMNE	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of trehaloseTRE	Fermentation of D-mannitolMAN	Fermentation of raffinoseRAF	Fermentation of D-riboseRIB	Fermentation of cellobioseCEL	Reduction of nitrateNIT	Acetoin production (Voges Proskauer test)VP	beta-Galactosidasebeta GAL	L-arginine arylamidaseArgA	Alkaline phosphatasePAL	Pyrrolidonyl arylamidasePyrA	Novobiocin resistanceNOVO	Fermentation of sucroseSAC	Fermentation of N-acetyl-glucosamineNAG	Fermentation of turanoseTUR	Fermentation of arabinoseARA	beta-Glucuronidasebeta GUR
[Ref.: #7650]	5909	+	-	-	-	+	+	-	+	-	+	+	-	-	-	+	+	+	-	+/-	-	+	+	+	+	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #122487]	8564	-	-	+	+	-	-	-	-	-	-	-	+	-	-	-	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #7650]	Sample type/isolated from	bovine nostril
[Ref.: #7650]	Geographic location (country and/or sea, region)	North Moravia
[Ref.: #7650]	Country	Czech Republic
[Ref.: #7650]	Country ISO 3 Code	CZE
[Ref.: #7650]	Continent	Europe

[Ref.: #122487]	Sample type/isolated from	Animal, Cow, nare
[Ref.: #122487]	Country	Czech Republic
[Ref.: #122487]	Country ISO 3 Code	CZE
[Ref.: #122487]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Host	#Mammals	#Bovinae (Cow, Cattle)
#Host Body-Site	#Oral cavity and airways	#Airways

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AB233327 (>99% sequence identity) for Staphylococcus from Microbeatlas

Aquatic Samples	1554
Soil Samples	930
Animal Samples	23674
Plant Samples	763
Total Samples	26921

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #7650]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #122487]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #7650]	Staphylococcus saprophyticus GTC 843 gene for 16S rRNA, partial sequence	AB233327	1456	GenBank ENA	147453 tax ID
[Ref.: #20218]	Staphylococcus saprophyticus subsp. bovis strain CCM 4410 16S ribosomal RNA gene, partial sequence	AY688088	464	GenBank ENA	147453 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Staphylococcus saprophyticus CCUG 38042	GCA_002902545	scaffold	GenBank ENA	29385 tax ID	*
[Ref.: #66792]	Staphylococcus saprophyticus subsp. bovis strain CCUG 38042	147453.4	wgs	PATRIC	29385 tax ID	*

[Ref.: #7650]

GC-content

31 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Staphylococcus saprophyticus subsp. bovis strain CCUG 38042 PATRIC: 147453.4
[Ref.: #69481]	spore-forming	spore-formingⓘ	no	99	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Staphylococcus saprophyticus CCUG 38042 NCBI: GCA_002902545.1
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	76.448	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	98.441	yes
[Ref.: #69480]	aerobic	aerobicⓘ	yes	69.917	no
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	71.882	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	95.1	no
[Ref.: #69480]	flagellated	motile2+ⓘ	no	80	no

Availability in culture collections External links

[Ref.: #7650]

Culture collection no.

DSM 18669, CCM 4410, CCUG 36975, CCUG 38042, CIP 105260

[Ref.: #83795]

SI-ID 57291

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Staphylococcus saprophyticus subsp. bovis subsp. nov., isolated from bovine nostrils.	Hajek V, Meugnier H, Bes M, Brun Y, Fiedler F, Chmela Z, Lasne Y, Fleurette J, Freney J	Int J Syst Bacteriol	10.1099/00207713-46-3-792	1996	*

References

#7650

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 18669

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#32746 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68375

Automatically annotated from API ID32STA .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#83795

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID57291.1 )

#122487 Collection of Institut Pasteur ; Curators of the CIP; CIP 105260
* These data were automatically processed and therefore are not curated

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Staphylococcus saprophyticus subsp. bovis strain CCUG 38042

Staphylococcus saprophyticus CCUG 38042

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Change proposal