Strain identifier

BacDive ID: 14669

Type strain: Yes

Species: Staphylococcus saprophyticus

Strain Designation: KV 12, KV 12T

Strain history: CIP <- 1997, J. Freney, Herriot Hosp., Lyon, France <- V. Hajek, Palacky Univ., Olomouc, Czech Republic: strain KV 12T

NCBI tax ID(s): 29385 (species)

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General

@ref: 7650

BacDive-ID: 14669

DSM-Number: 18669

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, Gram-positive, coccus-shaped

description: Staphylococcus saprophyticus KV 12 is a facultative anaerobe, Gram-positive, coccus-shaped bacterium that was isolated from bovine nostril.

NCBI tax id

  • NCBI tax id: 29385
  • Matching level: species

strain history

@refhistory
7650<- CCUG <- Z. Pácová, CCM <- V. Hájek
122487CIP <- 1997, J. Freney, Herriot Hosp., Lyon, France <- V. Hajek, Palacky Univ., Olomouc, Czech Republic: strain KV 12T

doi: 10.13145/bacdive14669.20240916.9.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Staphylococcaceae
  • genus: Staphylococcus
  • species: Staphylococcus saprophyticus
  • full scientific name: Staphylococcus saprophyticus (Fairbrother 1940) Shaw et al. 1951 (Approved Lists 1980)

  • synonyms

    @refsynonym
    20215Staphylococcus saprophyticus subsp. bovis
    20215Staphylococcus saprophyticus

@ref: 7650

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Staphylococcaceae

genus: Staphylococcus

species: Staphylococcus saprophyticus subsp. bovis

full scientific name: Staphylococcus saprophyticus subsp. bovis Hájek et al. 1996

strain designation: KV 12, KV 12T

type strain: yes

Morphology

cell morphology

  • @ref: 122487
  • gram stain: positive
  • cell shape: coccus-shaped
  • motility: no

colony morphology

@reftype of hemolysisincubation period
7650gamma1-2 days
122487

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
7650COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
7650TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
32746MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
122487CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3
122487CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperature
7650positivegrowth37
32746positivegrowth30
122487positivegrowth22-41
122487nogrowth10
122487nogrowth45

Physiology and metabolism

oxygen tolerance

  • @ref: 122487
  • oxygen tolerance: facultative anaerobe

spore formation

  • @ref: 69481
  • spore formation: no
  • confidence: 99

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
122487606565hippurate+hydrolysis
12248717632nitrate+reduction
12248716301nitrite-reduction
6837516199urea+hydrolysis
6837529016arginine-hydrolysis
6837518257ornithine-degradation
683754853esculin-hydrolysis
6837517634D-glucose+fermentation
6837515824D-fructose+fermentation
6837516024D-mannose-fermentation
6837517306maltose+fermentation
6837517716lactose-fermentation
6837527082trehalose+fermentation
6837516899D-mannitol+fermentation
6837516634raffinose-fermentation
6837516988D-ribose-fermentation
6837517057cellobiose-fermentation
6837517632nitrate+reduction
6837517992sucrose+fermentation
6837559640N-acetylglucosamine+fermentation
6837532528turanose+fermentation
6837522599arabinose-fermentation

antibiotic resistance

  • @ref: 68375
  • ChEBI: 28368
  • metabolite: novobiocin
  • is antibiotic: yes
  • is resistant: yes
  • resistance conc.: 1.8 µg

metabolite production

  • @ref: 68375
  • Chebi-ID: 15688
  • metabolite: acetoin
  • production: yes

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-test
6837515688acetoin+
12248715688acetoin-

enzymes

@refvalueactivityec
7650catalase+1.11.1.6
7650cytochrome-c oxidase-1.9.3.1
68375beta-glucuronidase-3.2.1.31
68375pyrrolidonyl arylamidase-3.4.19.3
68375L-arginine arylamidase-
68375beta-galactosidase+3.2.1.23
68375beta-glucosidase-3.2.1.21
68375ornithine decarboxylase-4.1.1.17
68375arginine dihydrolase-3.5.3.6
68375urease+3.5.1.5
122487oxidase-
122487beta-galactosidase+3.2.1.23
122487alcohol dehydrogenase-1.1.1.1
122487gelatinase+
122487amylase-
122487DNase-
122487caseinase-3.4.21.50
122487catalase+1.11.1.6
122487coagulase-
122487tween esterase-
122487gamma-glutamyltransferase-2.3.2.2
122487lecithinase-
122487lipase-
122487lysine decarboxylase-4.1.1.18
122487ornithine decarboxylase-4.1.1.17
122487phenylalanine ammonia-lyase-4.3.1.24
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase-3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase-3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
122487--++-------+---+----

API ID32STA

@refUREADH ArgODCESCGLUFRUMNEMALLACTREMANRAFRIBCELNITVPbeta GALArgAPALPyrANOVOSACNAGTURARAbeta GUR
7650+---++-+-++---+++-+/--++++--

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
122487++++---+-+++-------+--------+--+-+++-+---------------+-----++--------------+----------+---++-------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
7650bovine nostrilNorth MoraviaCzech RepublicCZEEurope
122487Animal, Cow, nareCzech RepublicCZEEurope

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Bovinae (Cow, Cattle)
#Host Body-Site#Oral cavity and airways#Airways

taxonmaps

  • @ref: 69479
  • File name: preview.99_63.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_3;97_3;98_3;99_63&stattab=map
  • Last taxonomy: Staphylococcus
  • 16S sequence: AB233327
  • Sequence Identity:
  • Total samples: 26921
  • soil counts: 930
  • aquatic counts: 1554
  • animal counts: 23674
  • plant counts: 763

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
76501Risk group (German classification)
1224871Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Staphylococcus saprophyticus subsp. bovis strain CCM 4410 16S ribosomal RNA gene, partial sequenceAY688088464nuccore147453
7650Staphylococcus saprophyticus GTC 843 gene for 16S rRNA, partial sequenceAB2333271456nuccore147453

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Staphylococcus saprophyticus CCUG 38042GCA_002902545scaffoldncbi29385
66792Staphylococcus saprophyticus subsp. bovis strain CCUG 38042147453.4wgspatric29385

GC content

  • @ref: 7650
  • GC-content: 31

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69481spore-formingspore-formingAbility to form endo- or exosporesno99no
69480gram-positivegram-positivePositive reaction to Gram-stainingyes76.448no
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no98.441yes
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)yes69.917no
69480spore-formingspore-formingAbility to form endo- or exosporesno71.882no
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cno95.1no
69480flagellatedmotile2+Ability to perform flagellated movementno80no

External links

@ref: 7650

culture collection no.: DSM 18669, CCM 4410, CCUG 36975, CCUG 38042, CIP 105260

straininfo link

  • @ref: 83795
  • straininfo: 57291

literature

  • topic: Phylogeny
  • Pubmed-ID: 8782691
  • title: Staphylococcus saprophyticus subsp. bovis subsp. nov., isolated from bovine nostrils.
  • authors: Hajek V, Meugnier H, Bes M, Brun Y, Fiedler F, Chmela Z, Lasne Y, Fleurette J, Freney J
  • journal: Int J Syst Bacteriol
  • DOI: 10.1099/00207713-46-3-792
  • year: 1996
  • mesh: Animals, Bacteriophage Typing, Base Composition, Cattle, Cell Wall, Fatty Acids/analysis, Microbial Sensitivity Tests, Nasal Cavity, Nucleic Acid Hybridization, Staphylococcus/chemistry/drug effects/*isolation & purification/physiology
  • topic2: Enzymology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
7650Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 18669)https://www.dsmz.de/collection/catalogue/details/culture/DSM-18669
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
32746Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17260
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68375Automatically annotated from API ID32STA
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
83795Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID57291.1StrainInfo: A central database for resolving microbial strain identifiers
122487Curators of the CIPCollection of Institut Pasteur (CIP 105260)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105260