Actinopolyspora salinaria HS05-03 | Type strain | BCC 51286, NBRC 109078, DSM 108651

Caption:

Medium 1240 37°C

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Information on culture and growth conditions Culture and growth conditions

Culture medium

ISP 4 agar

Culture medium growth

Culture medium

GYM STREPTOMYCES MEDIUM 10% NACL (DSMZ Medium 1159)

Culture medium growth

Culture medium link

Culture medium composition

Culture medium

STARCH-MINERAL SALT-AGAR (STMS) + 10%NACL (DSMZ Medium 1240)

Culture medium growth

Culture medium link

Culture medium composition

Culture medium

CM + YE MEDIUM (DSMZ Medium 549)

Culture medium growth

Culture medium link

Culture medium composition

Culture medium

CM-MEDIUM (DSMZ Medium 1504)

Culture medium growth

Culture medium link

Culture medium composition

		Temperature
[Ref.: #43990]	growth	22-41 °C
[Ref.: #43990]	optimum	37 °C
[Ref.: #66451]	growth	37 °C

	pH	Kind of pH		pH
[Ref.: #43990]			growth	5.0-8.0

Information on physiology and metabolism Physiology and metabolism

Oxygen tolerance

aerobe

Halophily / tolerance level

halophilic

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43990]		NaCl		growth	10-25	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43990]	(-)-D-fructose ChEBI	+	carbon source
[Ref.: #43990]	(-)-D-rhamnose	+	carbon source
[Ref.: #43990]	adenine ChEBI	-	hydrolysis
[Ref.: #43990]	allantoin ChEBI	-	hydrolysis
[Ref.: #43990]	arbutin ChEBI	+	hydrolysis
[Ref.: #43990]	beta-lactose ChEBI	+	carbon source
[Ref.: #43990]	casein	+	hydrolysis
[Ref.: #43990]	cellobiose ChEBI	+	carbon source
[Ref.: #43990]	cellulose ChEBI	-	hydrolysis
[Ref.: #43990]	D-galactose ChEBI	-	carbon source
[Ref.: #43990]	D-glucose ChEBI	+	carbon source
[Ref.: #43990]	D-ribose ChEBI	+	carbon source
[Ref.: #43990]	D-sorbitol ChEBI	+	carbon source
[Ref.: #43990]	D-trehalose ChEBI	-	carbon source
[Ref.: #43990]	D-xylose ChEBI	+	carbon source
[Ref.: #43990]	esculin ChEBI	+	hydrolysis
[Ref.: #43990]	glucose ChEBI	+	carbon source
[Ref.: #43990]	glycerol ChEBI	+	carbon source
[Ref.: #43990]	hypoxanthine ChEBI	-	hydrolysis
[Ref.: #43990]	L-arabinose ChEBI	+	carbon source
[Ref.: #43990]	L-tyrosine ChEBI	-	hydrolysis
[Ref.: #43990]	maltose ChEBI	+	carbon source
[Ref.: #43990]	melibiose ChEBI	-	carbon source
[Ref.: #43990]	nitrate ChEBI	+	reduction
[Ref.: #43990]	raffinose ChEBI	+	carbon source
[Ref.: #43990]	ribitol ChEBI	-	carbon source
[Ref.: #43990]	sorbose ChEBI	-	carbon source
[Ref.: #43990]	starch ChEBI	+	hydrolysis
[Ref.: #43990]	sucrose ChEBI	+	carbon source
[Ref.: #43990]	xanthine ChEBI	-	hydrolysis
[Ref.: #43990]	xylitol ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #43990]		hydrogen sulfide ChEBI	yes

	Enzyme	Enzyme activity	EC number
[Ref.: #43990]	acid phosphatase	+	3.1.3.2
[Ref.: #43990]	alkaline phosphatase	+	3.1.3.1
[Ref.: #43990]	alpha-fucosidase	-	3.2.1.51
[Ref.: #43990]	alpha-galactosidase	-	3.2.1.22
[Ref.: #43990]	alpha-glucosidase	-	3.2.1.20
[Ref.: #43990]	alpha-mannosidase	-	3.2.1.24
[Ref.: #43990]	beta-galactosidase	+	3.2.1.23
[Ref.: #43990]	beta-glucosidase	+	3.2.1.21
[Ref.: #43990]	beta-glucuronidase	-	3.2.1.31
[Ref.: #43990]	catalase	+	1.11.1.6
[Ref.: #43990]	chymotrypsin	-	3.4.4.5
[Ref.: #43990]	cystine arylamidase	-	3.4.11.3
[Ref.: #43990]	cytochrome oxidase	-	1.9.3.1
[Ref.: #43990]	esterase (C 4)	+
[Ref.: #43990]	esterase Lipase (C 8)	+
[Ref.: #43990]	leucine arylamidase	+	3.4.11.1
[Ref.: #43990]	lipase (C 14)	-
[Ref.: #43990]	N-acetyl-beta-glucosaminidase	+	3.2.1.52
[Ref.: #43990]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #43990]	trypsin	-	3.4.21.4
[Ref.: #43990]	urease	+/-	3.5.1.5
[Ref.: #43990]	valine arylamidase	-
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

What are isolation sources categories?

Fatty acid	Percentage
C16:0 iso	32.8
C17:0 anteiso	18.5
C15:0 iso	14.2
C17:0 iso	8.4
C15:0 anteiso	4.8
10-methyl C16:0	4.1
C14:0 iso	3.4
10-methyl C17:0	3.3
C17:0	1.4
C16:0	1.3
C16:1 iso H	1
10-methyl C18:0	0.9
C17:1ω8c	0.9
C14:0	0.7
C18:0	0.6
C18:0 iso	0.6
C18:1ω9c	0.4
C16:1ω7c / ω6c	0.3
C17:1 iso ω5c	0.3
C17:1 anteiso ω9c	0.2
C13:0 anteiso	0.2

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #43990]	Sample type/isolated from	solar saltern soil, Samut Sakhon province, Thailand
[Ref.: #43990]	Sampling date	2009-07
[Ref.: #43990]	Geographic location (country and/or sea, region)	Samut Sakhon province
[Ref.: #43990]	Country	Thailand
[Ref.: #43990]	Country ISO 3 Code	THA
[Ref.: #43990]	Continent	Asia
[Ref.: #43990]	Geographic location	13.4931°/100.1820°
[Ref.: #43990]	Enrichment culture	Humic acid-vitamin agar
[Ref.: #43990]	Enrichment culture composition	with artificial sea water containing 10 % (w/v) NaCl, supplemented with 25 mg/mL nalidixicacid, 100 mg/mL ketoconazole
[Ref.: #43990]	Enrichment culture duration	2 weeks
[Ref.: #43990]	Enrichment culture temperature	30 ̊C
* marker position based on {}

Isolation sources categories

#Condition

#Saline

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #43990]	Actinopolyspora sp. HS05-03 gene for 16S ribosomal RNA, partial sequence	AB894336		GenBank ENA

GC-content

69.9 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links