Strain identifier

BacDive ID: 141087

Type strain: Yes

Species: Actinopolyspora salinaria

Strain Designation: HS05-03

Strain history: <- NBRC; NBRC 109078 <- K. Duangmal, Kasetsart University, Thailand; HS05-03

NCBI tax ID(s): 1434887 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 9 (current version):
version 8.1:
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 9 (current version)

General

@ref: 66451

BacDive-ID: 141087

DSM-Number: 108651

keywords: 16S sequence, Bacteria, aerobe, halophilic, Gram-positive, filament-shaped

description: Actinopolyspora salinaria HS05-03 is an aerobe, halophilic, Gram-positive bacterium that was isolated from solar saltern soil, Samut Sakhon province, Thailand.

NCBI tax id

  • NCBI tax id: 1434887
  • Matching level: species

strain history

  • @ref: 66451
  • history: <- NBRC; NBRC 109078 <- K. Duangmal, Kasetsart University, Thailand; HS05-03

doi: 10.13145/bacdive141087.20240510.9

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Pseudonocardiales
  • family: Pseudonocardiaceae
  • genus: Actinopolyspora
  • species: Actinopolyspora salinaria
  • full scientific name: Actinopolyspora salinaria Duangmal et al. 2016

@ref: 66451

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinopolysporales

family: Actinopolysporaceae

genus: Actinopolyspora

species: Actinopolyspora salinaria

full scientific name: Actinopolyspora salinaria Duangmal et al. 2016

strain designation: HS05-03

type strain: yes

Morphology

cell morphology

  • @ref: 43990
  • gram stain: positive
  • cell shape: filament-shaped

colony morphology

  • @ref: 43990
  • colony color: white - pale yellow
  • medium used: ISP 4 agar

pigmentation

@refproductionname
43990nosoluble pigments
43990nomelanin pigments

multimedia

  • @ref: 66451
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_108651.jpg
  • caption: Medium 1240 37°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
43990ISP 4 agaryes
66451GYM STREPTOMYCES MEDIUM 10% NACL (DSMZ Medium 1159)yeshttps://mediadive.dsmz.de/medium/1159Name: GYM STREPTOMYCES MEDIUM 10% NACL (DSMZ Medium 1159) Composition: NaCl 100.0 g/l Agar 15.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
66451STARCH-MINERAL SALT-AGAR (STMS) + 10%NACL (DSMZ Medium 1240)yeshttps://mediadive.dsmz.de/medium/1240Name: STARCH-MINERAL SALT-AGAR + 10% NACL (DSMZ Medium 1240) Composition: NaCl 100.0 g/l Agar 15.0 g/l Starch 10.0 g/l (NH4)2SO4 2.0 g/l CaCO3 2.0 g/l K2HPO4 1.0 g/l MgSO4 x 7 H2O 1.0 g/l FeSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l ZnSO4 x 7 H2O 0.001 g/l Distilled water
66451CM + YE MEDIUM (DSMZ Medium 549)yeshttps://mediadive.dsmz.de/medium/549Name: CM + YE MEDIUM (DSMZ Medium 549) Composition: NaCl 200.0 g/l MgSO4 x 7 H2O 20.0 g/l Agar 15.0 g/l Yeast extract 10.0 g/l Casamino acids 7.5 g/l Na3-citrate 3.0 g/l KCl 2.0 g/l FeSO4 x 7 H2O 0.01 g/l HCl 3.646e-05 g/l Distilled water
66451CM-MEDIUM (DSMZ Medium 1504)yeshttps://mediadive.dsmz.de/medium/1504Name: CM MEDIUM (DSMZ Medium 1504) Composition: NaCl 150.0 g/l Agar 20.0 g/l MgSO4 x 7 H2O 10.0 g/l Yeast extract 10.0 g/l Peptone 7.5 g/l Na3-citrate 3.0 g/l KCl 2.0 g/l FeSO4 x 7 H2O Distilled water

culture temp

@refgrowthtypetemperature
43990positivegrowth22-41
43990positiveoptimum37
66451positivegrowth37

culture pH

  • @ref: 43990
  • ability: positive
  • type: growth
  • pH: 5.0-8.0

Physiology and metabolism

oxygen tolerance

  • @ref: 43990
  • oxygen tolerance: aerobe

halophily

  • @ref: 43990
  • halophily level: halophilic
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 10-25 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4399012936D-galactose-carbon source
4399015963ribitol-carbon source
4399016551D-trehalose-carbon source
4399028053melibiose-carbon source
4399027922sorbose-carbon source
4399016708adenine-hydrolysis
4399015676allantoin-hydrolysis
4399062968cellulose-hydrolysis
4399017368hypoxanthine-hydrolysis
4399017895L-tyrosine-hydrolysis
4399015318xanthine-hydrolysis
4399048095(-)-D-fructose+carbon source
43990(-)-D-rhamnose+carbon source
4399065327D-xylose+carbon source
4399030849L-arabinose+carbon source
4399036218beta-lactose+carbon source
4399017057cellobiose+carbon source
4399017634D-glucose+carbon source
4399016988D-ribose+carbon source
4399017924D-sorbitol+carbon source
4399017234glucose+carbon source
4399017754glycerol+carbon source
4399017306maltose+carbon source
4399016634raffinose+carbon source
4399017992sucrose+carbon source
4399017151xylitol+carbon source
4399018305arbutin+hydrolysis
43990casein+hydrolysis
439904853esculin+hydrolysis
4399028017starch+hydrolysis
4399017632nitrate+reduction

metabolite production

  • @ref: 43990
  • Chebi-ID: 16136
  • metabolite: hydrogen sulfide
  • production: yes

enzymes

@refvalueactivityec
43990cytochrome oxidase-1.9.3.1
43990catalase+1.11.1.6
43990acid phosphatase+3.1.3.2
43990alkaline phosphatase+3.1.3.1
43990beta-galactosidase+3.2.1.23
43990beta-glucosidase+3.2.1.21
43990esterase (C 4)+
43990leucine arylamidase+3.4.11.1
43990esterase Lipase (C 8)+
43990N-acetyl-beta-glucosaminidase+3.2.1.52
43990naphthol-AS-BI-phosphohydrolase+
43990alpha-fucosidase-3.2.1.51
43990alpha-galactosidase-3.2.1.22
43990alpha-glucosidase-3.2.1.20
43990beta-glucuronidase-3.2.1.31
43990alpha-mannosidase-3.2.1.24
43990chymotrypsin-3.4.4.5
43990cystine arylamidase-3.4.11.3
43990lipase (C 14)-
43990trypsin-3.4.21.4
43990valine arylamidase-
43990urease+/-3.5.1.5

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    43990C13:0 anteiso0.2
    43990C15:0 anteiso4.8
    43990C17:0 anteiso18.5
    43990C17:1 anteiso ω9c0.2
    43990C14:00.7
    43990C16:01.3
    4399010-methyl C16:04.1
    43990C16:1ω7c / ω6c0.3
    43990C17:01.4
    4399010-methyl C17:03.3
    43990C17:1ω8c0.9
    43990C18:00.6
    4399010-methyl C18:00.9
    43990C18:1ω9c0.4
    43990C14:0 iso3.4
    43990C15:0 iso14.2
    43990C16:0 iso32.8
    43990C16:1 iso H1
    43990C17:0 iso8.4
    43990C17:1 iso ω5c0.3
    43990C18:0 iso0.6
  • type of FA analysis: whole cell analysis
  • incubation medium: ISP 4 medium
  • agar/liquid: liquid
  • incubation temperature: 37
  • incubation time: 15
  • software version: Sherlock 6.1
  • library/peak naming table: RTSBA6
  • system: MIS MIDI
  • method/protocol: Sasser, 1990

Isolation, sampling and environmental information

isolation

  • @ref: 43990
  • sample type: solar saltern soil, Samut Sakhon province, Thailand
  • sampling date: 2009-07
  • geographic location: Samut Sakhon province
  • country: Thailand
  • origin.country: THA
  • continent: Asia
  • latitude: 13.4931
  • longitude: 100.182
  • enrichment culture: Humic acid-vitamin agar
  • enrichment culture composition: with artificial sea water containing 10 % (w/v) NaCl, supplemented with 25 mg/mL nalidixicacid, 100 mg/mL ketoconazole
  • enrichment culture duration: 2 weeks
  • enrichment culture temperature: 30

isolation source categories

  • Cat1: #Condition
  • Cat2: #Saline

Safety information

risk assessment

  • @ref: 66451
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 43990
  • description: Actinopolyspora sp. HS05-03 gene for 16S ribosomal RNA, partial sequence
  • accession: AB894336
  • database: nuccore

GC content

  • @ref: 66451
  • GC-content: 69.9
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 66451

culture collection no.: BCC 51286, NBRC 109078, DSM 108651

straininfo link

  • @ref: 96852
  • straininfo: 401895

literature

  • topic: Phylogeny
  • Pubmed-ID: 26812900
  • title: Actinopolyspora salinaria sp. nov., a halophilic actinomycete isolated from solar saltern soil.
  • authors: Duangmal K, Suksaard P, Pathom-Aree W, Mingma R, Matsumoto A, Takahashi Y
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.000926
  • year: 2016
  • mesh: Actinomycetales/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Thailand, Vitamin K 2/chemistry
  • topic2: Genetics

Reference

@idauthorstitledoi/urljournalpubmedcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
43990Kannika Duangmal, Paweena Suksaard, Wasu Pathom-aree, Ratchanee Mingma, Atsuko Matsumoto, Yoko TakahashiActinopolyspora salinaria sp. nov., a halophilic actinomycete isolated from solar saltern soil10.1099/ijsem.0.000926IJSEM 66: 1660-1665 201626812900
66451Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-108651Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 108651)
96852Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID401895.1