Alkalispirochaeta cellulosivorans (KCTC 15343, NBRC 110105, JC227)

Name: Alkalispirochaeta cellulosivorans (KCTC 15343, NBRC 110105, JC227)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 141088

Type strain

Strain Designation JC227

Culture col. no. KCTC 15343 NBRC 110105 JC227

NCBI tax ID(s) 1410329

Special Collections KCTC Literature strains

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Alkalispirochaeta cellulosivorans JC227 is an obligate anaerobe, mesophilic, Gram-negative prokaryote that was isolated from gut of a wood-eating cockroach, Cryptocercus punctulatus , from the Rann of Kutch, Gujarat, India.

Gram-negative motile spiral-shaped obligate anaerobe mesophilic 16S sequence

Name and taxonomic classification

SI-ID 287787

KCTC 15343,NBRC 110105

@ref 20215

Last LPSN update 2025-12-16 10:03:46

Domain Pseudomonadati

Phylum Spirochaetota

Class Spirochaetia

Order Spirochaetales

Family Spirochaetaceae

Genus Alkalispirochaeta

Species Alkalispirochaeta cellulosivorans

Full scientific name Alkalispirochaeta cellulosivorans Sravanthi et al. 2016

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape
43991	negative	10-20 µm	0.1-0.2 µm	spiral-shaped
67771
67771	negative

Culture and growth conditions

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
43991	positive	optimum	30-38	mesophilic
43991	positive	growth	15-40
67771	positive	growth	37	mesophilic

Culture pH

@ref	Ability	Type	PH	PH range
43991	positive	optimum	9.5-10.0
43991	positive	growth	9.0-11.0	alkaliphile

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
43991	obligate anaerobe
67771	anaerobe

Halophily

@ref	Salt	Growth	Tested relation	Concentration
43991	NaCl	positive	growth	0-10 %(w/v)
43991	NaCl	positive	optimum	1 %(w/v)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
43991	30089 ChEBI	acetate	-	growth
43991	22651 ChEBI	ascorbate	+	growth
43991	16150 ChEBI	benzoate	-	growth
43991		casamino acids	+	growth
43991	17057 ChEBI	cellobiose	+	growth
43991	62968 ChEBI	cellulose	+	growth
43991	15824 ChEBI	D-fructose	+	growth
43991	17634 ChEBI	D-glucose	+	growth
43991	16899 ChEBI	D-mannitol	+	growth
43991	16236 ChEBI	ethanol	-	growth
43991	29806 ChEBI	fumarate	-	growth
43991	17754 ChEBI	glycerol	-	growth
43991	29805 ChEBI	glycolate	-	growth
43991	24996 ChEBI	lactate	+	growth
43991		peptone	+	growth
43991	30031 ChEBI	succinate	-	growth
43991	17992 ChEBI	sucrose	+	growth
43991	18222 ChEBI	xylose	+	growth
43991		yeast extract (0.01 %, w/v)	+	growth

Antibiotic resistance

@ref	ChEBI	Metabolite	Sensitivity conc.	Resistance conc.
43991	28971	ampicillin	50 µg/mL
43991	17698	chloramphenicol	50 µg/mL
43991	17833	gentamicin	100 µg/mL
43991	6104	kanamycin		100 µg/mL
43991	28077	rifampicin		100 µg/mL
43991	27902	tetracycline	100 µg/mL

Fatty acid profile

Metadata FA analysis

@ref

43991

Fatty acid	Percentage
C18:1ω7c / C18:1ω6c	32.8
C16:0	20.3
C14:0	18
C16:1ω5c	8.9
C15:1ω6c	6.3
C12:0	4.1
C17:0 2OH	1.9
C16:1ω6c / C16:1ω7c	1.3

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Arthropoda	#Insecta
#Host Body-Site	#Gastrointestinal tract	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Latitude	Longitude	Enrichment culture	Enrichment culture composition
43991	gut of a wood-eating cockroach, Cryptocercus punctulatus (Scudder), from the Rann of Kutch, Gujarat, India	Rann of Kutch, Gujarat	India	IND	Asia	23.8478	69.7316 23.8478/69.7316	basic alkaline medium	Na2CO3 (2 g/L), NaHCO3 (4.5 g/L); NaCl (48 g/L); NH4Cl (0.5 g/L); KH2PO4 (0.3 g/L); MgCl2.6H2O (0.2 g/L); resazurin (0.001 g/L); yeast extract (0.5 g/L); sucrose (5 g/L); SL7 (trace elements solution; 1 ml) [SL7 contained HCl (25 %, v/v) (1 ml); ZnCl2 (70 mg/L); MnCl2.4H2O (100 mg/L); H3BO3 (60 mg/L); CoCl2.6H2O (200 mg/L) NiCl2.6H2O (20 mg/L); Na2MoO4.2H2O (40 mg/L); CuCl2.2H2O (20 mg/L)] and 10 ml vitamin solution, Na2S.9H2O (0.5 g/L), pH 11-12.5
67771	From gut of a wood eating cockroach, `Cryptocercus punctulatus`(Scudder)	the Rann of Kutch, Gujarat	India	IND	Asia

Taxonmaps

69479

Global distribution of 16S sequence HG531387 (>99% sequence identity) for Alkalispirochaeta from Microbeatlas

Aquatic Samples	304
Soil Samples	26
Animal Samples	52
Plant Samples
Total Samples	382

Interaction and safety

Sequence information

16S sequences

@ref	Description	Accession	Database
43991	Spirochaeta sp. JC227 partial 16S rRNA gene, strain JC227	HG531387

GC content

@ref	GC-content (mol%)	Method
43991	55.5	high performance liquid chromatography (HPLC)
67771	55.5

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Alkalispirochaeta cellulosivorans gen. nov., sp. nov., a cellulose-hydrolysing, alkaliphilic, halotolerant bacterium isolated from the gut of a wood-eating cockroach (Cryptocercus punctulatus), and reclassification of four species of Spirochaeta as new combinations within Alkalispirochaeta gen. nov.	Sravanthi T, Tushar L, Sasikala C, Ramana CV	Int J Syst Evol Microbiol	10.1099/ijsem.0.000865	2015

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#43991	T. Sravanthi, L. Tushar, Ch. Sasikala, Ch. V. Ramana: Alkalispirochaeta cellulosivorans gen. nov., sp. nov., a cellulose-hydrolysing, alkaliphilic, halotolerant bacterium isolated from the gut of a wood-eating cockroach (Cryptocercus punctulatus), and reclassification of four species of Spirochaeta as new combinations within Alkalispirochaeta gen. nov.. IJSEM 66: 1612 - 1619 2016 ( DOI 10.1099/ijsem.0.000865 , PubMed 26704619 )
#67771	Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Alkalispirochaeta cellulosivorans (KCTC 15343, NBRC 110105, JC227)

Name and taxonomic classification