Name: Alkalispirochaeta cellulosivorans JC227
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 141088

Type strain:

Species: Alkalispirochaeta cellulosivorans

Strain Designation: JC227

Culture col. no.: KCTC 15343, NBRC 110105

Strain history: <- Ch Sasikala, JNT Univ, India

NCBI tax ID(s): 1410329 (species)

Section

Alkalispirochaeta cellulosivorans JC227 is an obligate anaerobe, mesophilic, Gram-negative bacterium that was isolated from gut of a wood-eating cockroach, Cryptocercus punctulatus , from the Rann of Kutch, Gujarat, India.

Gram-negative
motile
spiral-shaped
mesophilic
obligate anaerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Spirochaetota*
	Class	*Spirochaetia*
	Order	*Spirochaetales*
	Family	*Spirochaetaceae*
	Genus	*Alkalispirochaeta*
	Species	**Alkalispirochaeta cellulosivorans**
	Full Scientific Name (LPSN)	Alkalispirochaeta cellulosivorans Sravanthi et al. 2016

Information on morphological and physiological properties Morphology

[Ref.: #43991]	Gram stain	negative
[Ref.: #67771]	Gram stain	negative

[Ref.: #43991]	Cell length	10-20 µm

[Ref.: #43991]	Cell width	0.1-0.2 µm

[Ref.: #43991]	Cell shape	spiral-shaped

[Ref.: #43991]	Motility	yes
[Ref.: #67771]	Motility	yes

Information on culture and growth conditions Culture and growth conditions

		Temperature
[Ref.: #43991]	optimum	30-38 °C
[Ref.: #43991]	growth	15-40 °C
[Ref.: #67771]	growth	37 °C

[Ref.: #43991]	Temperature range	mesophilic
[Ref.: #67771]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #43991]			optimum	9.5-10.0
[Ref.: #43991]			growth	9.0-11.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43991]	Oxygen tolerance	obligate anaerobe
[Ref.: #67771]	Oxygen tolerance	anaerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43991]		NaCl		growth	0-10	%(w/v)
[Ref.: #43991]		NaCl		optimum	1	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43991]	acetate ChEBI	-	growth
[Ref.: #43991]	ascorbate ChEBI	+	growth
[Ref.: #43991]	benzoate ChEBI	-	growth
[Ref.: #43991]	casamino acids	+	growth
[Ref.: #43991]	cellobiose ChEBI	+	growth
[Ref.: #43991]	cellulose ChEBI	+	growth
[Ref.: #43991]	D-fructose ChEBI	+	growth
[Ref.: #43991]	D-glucose ChEBI	+	growth
[Ref.: #43991]	D-mannitol ChEBI	+	growth
[Ref.: #43991]	ethanol ChEBI	-	growth
[Ref.: #43991]	fumarate ChEBI	-	growth
[Ref.: #43991]	glycerol ChEBI	-	growth
[Ref.: #43991]	glycolate ChEBI	-	growth
[Ref.: #43991]	lactate ChEBI	+	growth
[Ref.: #43991]	peptone	+	growth
[Ref.: #43991]	succinate ChEBI	-	growth
[Ref.: #43991]	sucrose ChEBI	+	growth
[Ref.: #43991]	xylose ChEBI	+	growth
[Ref.: #43991]	yeast extract (0.01 %, w/v)	+	growth
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance	Concentration
[Ref.: #43991]	ampicillin ChEBI	yes	50	µg/mL
[Ref.: #43991]	chloramphenicol ChEBI	yes	50	µg/mL
[Ref.: #43991]	gentamicin ChEBI	yes	100	µg/mL
[Ref.: #43991]	kanamycin ChEBI				yes	100	µg/mL
[Ref.: #43991]	rifampicin ChEBI				yes	100	µg/mL
[Ref.: #43991]	tetracycline ChEBI	yes	100	µg/mL

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #43991]

Fatty acid	Percentage
C18:1ω7c / C18:1ω6c	32.8
C16:0	20.3
C14:0	18
C16:1ω5c	8.9
C15:1ω6c	6.3
C12:0	4.1
C17:0 2OH	1.9
C16:1ω6c / C16:1ω7c	1.3

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #43991]	Sample type/isolated from	gut of a wood-eating cockroach, Cryptocercus punctulatus (Scudder), from the Rann of Kutch, Gujarat, India
[Ref.: #43991]	Geographic location (country and/or sea, region)	Rann of Kutch, Gujarat
[Ref.: #43991]	Country	India
[Ref.: #43991]	Country ISO 3 Code	IND
[Ref.: #43991]	Continent	Asia
[Ref.: #43991]	Geographic location	23.8478°/69.7316°
[Ref.: #43991]	Enrichment culture	basic alkaline medium
[Ref.: #43991]	Enrichment culture composition	Na2CO3 (2 g/L), NaHCO3 (4.5 g/L); NaCl (48 g/L); NH4Cl (0.5 g/L); KH2PO4 (0.3 g/L); MgCl2.6H2O (0.2 g/L); resazurin (0.001 g/L); yeast extract (0.5 g/L); sucrose (5 g/L); SL7 (trace elements solution; 1 ml) [SL7 contained HCl (25 %, v/v) (1 ml); ZnCl2 (70 mg/L); MnCl2.4H2O (100 mg/L); H3BO3 (60 mg/L); CoCl2.6H2O (200 mg/L) NiCl2.6H2O (20 mg/L); Na2MoO4.2H2O (40 mg/L); CuCl2.2H2O (20 mg/L)] and 10 ml vitamin solution, Na2S.9H2O (0.5 g/L), pH 11-12.5

[Ref.: #67771]	Sample type/isolated from	From gut of a wood eating cockroach, `Cryptocercus punctulatus`(Scudder)
[Ref.: #67771]	Geographic location (country and/or sea, region)	the Rann of Kutch, Gujarat
[Ref.: #67771]	Country	India
[Ref.: #67771]	Country ISO 3 Code	IND
[Ref.: #67771]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Host	#Arthropoda	#Insecta
#Host Body-Site	#Gastrointestinal tract	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence HG531387 (>99% sequence identity) for Alkalispirochaeta from Microbeatlas

Aquatic Samples	304
Soil Samples	26
Animal Samples	52
Plant Samples
Total Samples	382

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #43991]	16S rRNA gene sequence	HG531387		GenBank

[Ref.: #43991]	GC-content	55.5 mol%	high performance liquid chromatography (HPLC)
[Ref.: #67771]	GC-content	55.5 mol%

Availability in culture collections External links

[Ref.: #43991]

Culture collection no.

KCTC 15343, NBRC 110105

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Alkalispirochaeta cellulosivorans gen. nov., sp. nov., a cellulose-hydrolysing, alkaliphilic, halotolerant bacterium isolated from the gut of a wood-eating cockroach (Cryptocercus punctulatus), and reclassification of four species of Spirochaeta as new combinations within Alkalispirochaeta gen. nov.	Sravanthi T, Tushar L, Sasikala C, Ramana CV	Int J Syst Evol Microbiol	10.1099/ijsem.0.000865	2015	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#43991

T. Sravanthi, L. Tushar, Ch. Sasikala, Ch. V. Ramana: Alkalispirochaeta cellulosivorans gen. nov., sp. nov., a cellulose-hydrolysing, alkaliphilic, halotolerant bacterium isolated from the gut of a wood-eating cockroach (Cryptocercus punctulatus), and reclassification of four species of Spirochaeta as new combinations within Alkalispirochaeta gen. nov.. IJSEM 66: 1612 - 1619 2016 ( DOI 10.1099/ijsem.0.000865 , PubMed 26704619 )

#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

* These data were automatically processed and therefore are not curated

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